Purification and Characterization of Recombinant Darbepoetin Alfa from Leishmania tarentolae

Darbepoetin alfa is a biopharmaceutical glycoprotein that stimulates erythropoiesis and is used to treat anemia, which associated with renal failure and cancer chemotherapy. We herein describe the structural characterization of recombinant darbepoetin alfa produced by Leishmania tarentolae T7-TR host. The DNA expression cassette was integrated into the L. tarentolae genome through homologous recombination. Transformed clones were selected by antibiotic resistance, diagnostic PCRs, and protein expression analysis. The structure of recombinant darbepoetin alfa was analyzed by isoelectric focusing, ultraviolet–visible spectrum, and circular dichroism (CD) spectroscopy. Expression analysis showed the presence of a protein band at 40 kDa, and its expression level was 51.2 mg/ml of culture medium. Darbepoetin alfa have 5 isoforms with varying degree of sialylation. The UV absorption and CD spectra were analogous to original drug (Aranesp), which confirmed that the produced protein was darbepoetin alfa. Potency test results revealed that the purified protein was biologically active. In brief, the structural and biological characteristics of expressed darbepoetin alfa were very similar to Aranesp which has been normally expressed in CHO. Our data also suggest that produced protein has potential to be developed for clinical use. © 2016, Springer Science+Business Media New York

Detection of microcalcifications in mammograms using higher order statistics

Cataloged from PDF version of article.A new method for detecting microcalcifications in mammograms is described. In this method, the mammogram image is first processed by a subband decomposition filterbank. The bandpass subimage is divided into overlapping square regions in which skewness and kurtosis as measures of the asymmetry and impulsiveness of the distribution are estimated. The detection method utilizes these two parameters. A region with high positive skewness and kurtosis is marked as a region of interest. Simulation results show that this method is successful in detecting regions with microcalcifications

Full counting statistics of information content

We review connections between the cumulant generating function of full counting statistics of particle number and the R\'enyi entanglement entropy. We calculate these quantities based on the fermionic and bosonic path-integral defined on multiple Keldysh contours. We relate the R\'enyi entropy with the information generating function, from which the probability distribution function of self-information is obtained in the nonequilibrium steady state. By exploiting the distribution, we analyze the information content carried by a single bosonic particle through a narrow-band quantum communication channel. The ratio of the self-information content to the number of bosons fluctuates. For a small boson occupation number, the average and the fluctuation of the ratio are enhanced.Comment: 16 pages, 5 figure

Microlensing toward crowded fields: Theory and applications to M31

We present a comprehensive treatment of the pixel-lensing theory and apply it to lensing experiments and their results toward M31. Using distribution functions for the distances, velocities, masses, and luminosities of stars, we derive lensing event rates as a function of the event observables. In contrast to the microlensing regime, in the pixel-lensing regime (crowded or unresolved sources) the observables are the maximum excess flux of the source above a background and the full width at half-maximum (FWHM) time of the event. To calculate lensing event distribution functions depending on these observables for the specific case of M31, we use data from the literature to construct a model of M31, reproducing consistently photometry, kinematics and stellar population. We predict the halo- and self-lensing event rates for bulge and disk stars in M31 and treat events with and without finite source signatures separately. We use the M31 photon noise profile and obtain the event rates as a function of position, field of view, and S/N threshold at maximum magnification. We calculate the expected rates for WeCAPP and for a potential Advanced Camera for Surveys (ACS) lensing campaign. The detection of two events with a peak signal-to-noise ratio larger than 10 and a timescale larger than 1 day in the WeCAPP 2000/2001 data is in good agreement with our theoretical calculations. We investigate the luminosity function of lensed stars for noise characteristics of WeCAPP and ACS. For the pixel-lensing regime, we derive the probability distribution for the lens masses in M31 as a function of the FWHM timescale, flux excess and color, including the errors of these observables.Comment: 45 pages, 27 figures LaTeX; corrected typos; published in the Astrophysical Journal Supplemen

Expected Future Precipitation in Central Iraq Using LARS-WG Stochastic Weather Generator

The Middle East (ME) is characterized by its water shortage problem. This region with its arid climate is expected to be the most vulnerable in the world to the potential impacts of climate change. Iraq (located in ME) is seriously experiencing water shortage problem. To overcome this problem rain water harvesting can be used. In this study the applicability of the long-term weather generator model in downscaling daily precipitation Central Iraq is used to project future changes of precipitation based on scenario of seven General Circulation Models (GCMs) outputs for the periods of 2011-2030, 2046-2065, and 2080-2099. The results indicated that December-February and September-November periods, based on the ensemble mean of seven GCMs, showed an increasing trend in the periods considered; however, a decreasing trend can be found in March, April, and May in the future

CLIMATE CHANGE AND FUTURE PRECIPITATION IN AN ARID ENVIRONMENT OF THE MIDDLE EAST: CASE STUDY OF IRAQ

In this paper impact of climate change on precipitation in the arid environment of Iraq is examined. LARS-WG weather generator was applied to 5 representative regions to model current and future precipitation under climate change. Seven Global Climate Models (GCMs) have been employed to account for any uncertainty on future projection for three selected periods, 2011-2030, 2046-2065 and 2080-2099. Performance of LARS-WG in each site was first evaluated using the Kolmogorov-Smirnov statistical test for fitting wet/dry days in each site, as well as comparison of the mean and standard deviation between the observed and simulated precipitation. The developed LARS-WG models were found to perform well and skilful in simulating precipitation in the arid regions of Iraq as evidenced by the tests carried and the comparison made. The precipitation models were then used to obtain future projections for precipitation using the IPCC scenario SRES A2. Future precipitation results show that most of the Iraq regions are projected to suffer a reduction in annual mean precipitation, especially by the end of the 21st century, while on a seasonal basis most of the regions are anticipated to be wetter in autumn and winter. Journa

AgapeZ1: a Large Amplification Microlensing Event or an Odd Variable Star Towards the Inner Bulge of M31

AgapeZ1 is the brightest and the shortest duration microlensing candidate event found in the Agape data. It occured only 42" from the center of M31. Our photometry shows that the half intensity duration of the event6 is 4.8 days and at maximum brightness we measure a stellar magnitude of R=18.0 with B-R=0.80 mag color. A search on HST archives produced a single resolved star within the projected event position error box. Its magnitude is R=22.Comment: 4 pages with 5 figure

AGAPE, an experiment to detect MACHO's in the direction of the Andromeda galaxy

The status of the Agape experiment to detect Machos in the direction of the andromeda galaxy is presented.Comment: 4 pages, 1 figure in a separate compressed, tarred, uuencoded uufile. In case ofproblem contact [email protected]