55 research outputs found
Effects of soil warming and nitrogen foliar applications on bud burst of black spruce
Key message: In mature black spruce, bud burst process is anticipated by soil warming, while delayed by foliar applications of nitrogen; however, the effects depend on growth conditions at the site.
Abstract: The observation of phenological events can be used as biological indicator of environmental changes, especially from the perspective of climate change. In boreal forests, the onset of the bud burst is a key factor in the length of the growing season. With current climate change, the major factors limiting the growth of boreal trees (i.e., temperature and nitrogen availability) are changing and studies on mature trees are limited. The aim of this study was to investigate the effects of soil warming and increased nitrogen (N) deposition on bud burst of mature black spruce [Picea mariana (Mill.) BSP]. From 2008 onwards, an experimental manipulation of these environmental growth conditions was conducted in two stands (BER and SIM) at different altitudes in the boreal forest of Quebec, Canada. An increase in soil temperature (H treatment) and a canopy application of artificial rain enriched with nitrogen (N treatment) were performed. Observations of bud phenology were made during May–July 2012 and 2013. In BER, H treatment caused an anticipation (estimated as 1–3 days); while N treatment, a delay (estimated as 1–2 days but only in 2012) in bud burst. No treatments effect was significant in SIM. It has been demonstrated that soil temperature and N availability can play an important role in affecting bud burst in black spruce but the effects of these environmental factors on growth are closely linked with site conditions
Finishing the euchromatic sequence of the human genome
The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead
‘Oh you pretty thing!’: How David Bowie ‘unlocked everybody’s inner queen’ in spite of the music press
The 1967 Sexual Offence Act decriminalised homosexual acts between men allowing gay men to discuss their sexuality in public. Few prominent popular musicians came-out until 1972 when David Bowie claimed that he was bisexual in an interview with Melody Maker. Music papers and Bowie had substantial cultural power: Bowie was a rising star and music papers recruited journalists who discussed and perpetuated social change. The subsequent conversation, however, reinforced negative stereotypes in constructing the queer subject and tried to safeguard commercial concerns due to the assumption that the market for popular music avoided queer music. This undermined arguments that associate permissive legislation with a permissive media and society, but, to some, representation alone empowered people and destabilised preconceptions about queer identity.Published versio
Human motoneurone excitability is depressed by activation of 5HT1A receptors with buspirone
Key points: In the adult turtle spinal cord, action potential generation in motoneurones is inhibited by spillover of serotonin to extrasynaptic serotonin 1A (5-HT) receptors at the axon initial segment. We explored whether ingestion of the 5-HT receptor partial agonist, buspirone, decreases motoneurone excitability in humans. Following ingestion of buspirone, two tests of motoneurone excitability showed decreases. F-wave areas and persistence in an intrinsic muscle of the hand were reduced, as was the area of cervicomedullary motor evoked potentials in biceps brachii. Our findings suggest that activation of 5-HT receptors depresses human motoneurone excitability. Such a depression could contribute to decreased motoneurone output during fatiguing exercise if there is high serotonergic drive to the motoneurones. Abstract: Intense serotonergic drive in the turtle spinal cord results in serotonin spillover to the axon initial segment of the motoneurones where it activates serotonin 1A (5-HT) receptors and inhibits generation of action potentials. We examined whether activation of 5-HT receptors decreases motoneurone excitability in humans by determining the effects of a 5-HT receptor partial agonist, buspirone, on F waves and cervicomedullary motor evoked potentials (CMEPs). In a placebo-controlled double-blind study, 10 participants were tested on two occasions where either placebo or 20\ua0mg of buspirone was administered orally. The ulnar nerve was stimulated supramaximally to evoke F waves in abductor digiti minimi (ADM). CMEPs and the maximal M wave were elicited in biceps brachii by cervicomedullary stimulation and brachial plexus stimulation, respectively. Following buspirone intake, F-wave area and persistence, as well as CMEP area, were significantly decreased. The mean post-pill difference in normalized F-wave areas and persistence between buspirone and placebo days was –27% (–42, –12; 95% confidence interval) and –9% (–16, –2), respectively. The mean post-pill difference in normalized CMEP area between buspirone and placebo days showed greater variation and was –31% (–60, –2). In conclusion, buspirone reduces motoneurone excitability in humans probably via activation of 5-HT receptors at the axon initial segment. This has implications for motor output during high drive to the motoneurones when serotonin may spill over to these inhibitory receptors and consequently inhibit motoneurone output. Such a mechanism could potentially contribute to fatigue with exercise
Machine learning of large‐scale spatial distributions of wild turkeys with high‐dimensional environmental data
Abstract Species distribution modeling often involves high‐dimensional environmental data. Large amounts of data and multicollinearity among covariates impose challenges to statistical models in variable selection for reliable inferences of the effects of environmental factors on the spatial distribution of species. Few studies have evaluated and compared the performance of multiple machine learning (ML) models in handling multicollinearity. Here, we assessed the effectiveness of removal of correlated covariates and regularization to cope with multicollinearity in ML models for habitat suitability. Three machine learning algorithms maximum entropy (MaxEnt), random forests (RFs), and support vector machines (SVMs) were applied to the original data (OD) of 27 landscape variables, reduced data (RD) with 14 highly correlated covariates being removed, and 15 principal components (PC) of the OD accounting for 90% of the original variability. The performance of the three ML models was measured with the area under the curve and continuous Boyce index. We collected 663 nonduplicated presence locations of Eastern wild turkeys (Meleagris gallopavo silvestris) across the state of Mississippi, United States. Of the total locations, 453 locations separated by a distance of ≥2 km were used to train the three ML algorithms on the OD, RD, and PC data, respectively. The remaining 210 locations were used to validate the trained ML models to measure ML performance. Three ML models had excellent performance on the RD and PC data. MaxEnt and SVMs had good performance on the OD data, indicating the adequacy of regularization of the default setting for multicollinearity. Weak learning of RFs through bagging appeared to alleviate multicollinearity and resulted in excellent performance on the OD data. Regularization of ML algorithms may help exploratory studies of the effects of environmental factors on the spatial distribution and habitat suitability of wildlife
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Magnetic Field-Induced Switching of the Radical-Pair Intersystem Crossing Mechanism in a Donor−Bridge−Acceptor Molecule for Artificial Photosynthesis
A covalent, fixed-distance donor−bridge−acceptor (D−B−A) molecule was synthesized that upon photoexcitation undergoes ultrafast charge separation to yield a radical ion pair (RP) in which the spin−spin exchange interaction (2J) between the two radicals is sufficiently large to result in preferential RP intersystem crossing to the highest-energy RP eigenstate (T+1) at the 350 mT magnetic field characteristic of X-band (9.5 GHz) EPR spectroscopy. This behavior is unprecedented in covalent D−B−A molecules, and is evidenced by the time-resolved EPR (TREPR) spectrum at X-band of 3*D−B−A derived from RP recombination, which shows all six canonical EPR transitions polarized in emission (e,e,e,e,e,e). In contrast, when the RP is photogenerated in a 3400 mT magnetic field, the TREPR triplet spectrum at W-band (94 GHz) of 3*D−B−A displays the (a,e,e,a,a,e) polarization pattern characteristic of a weakly coupled RP precursor, similar to that observed in photosynthetic reaction center proteins, and indicates a switch to selective population of the lower-energy T0 eigenstate
Photoinitiated Electron Transfer in Zinc Porphyrin–Perylenediimide Cruciforms and Their Self-Assembled Oligomers
Two X-shaped, cruciform electron donor<sub>2</sub>–acceptor–acceptor′<sub>2</sub> (D<sub>2</sub>-A-A′<sub>2</sub>) molecules, <b>1</b> and <b>2</b>, in which D = zinc 5-phenyl-10,15,20-tripentylporphyrin
(ZnTPnP) or zinc 5,10,15,20-tetraphenylporphyrin (ZnTPP), respectively,
A = pyromellitimide (PI), and A′ = perylene-3,4:9,10-bis(dicarboximide)
(PDI), were prepared to study self-assembly motifs that promote photoinitiated
charge separation followed by electron and hole transport through
π-stacked donors and acceptors. PDI secondary electron acceptors
were chosen because of their propensity to form self-ordered, π-stacked
assemblies in solution, while the ZnTPnP and ZnTPP donors were selected
to test the effect of peripheral substituent steric interactions on
the π-stacking characteristics of the cruciforms. Small- and
wide-angle X-ray scattering measurements in toluene solution reveal
that <b>1</b> assembles into a π-stacked structure having
an average of 5 ± 1 molecules, when [<b>1</b>] ≅
10<sup>–5</sup> M, while <b>2</b> remains monomeric.
Photoexcitation of the π-stacked structure of <b>1</b> results in formation of ZnTPnP<sup>•+</sup>-PI-PDI<sup>•–</sup> in τ<sub>CS1</sub> = 0.3 ps, which is nearly 100-fold faster
than the formation of ZnTPnP<sup>•+</sup>-PI<sup>•–</sup> in a model system lacking the PDI acceptor. The data are consistent
with a self-assembled structure for <b>1</b> in which the majority
of the intermolecular interactions have the ZnTPnP donor of one monomer
cofacially π-stacked with the PDI acceptor of a neighboring
monomer in a crisscrossed fashion. In contrast, <b>2</b> remains
monomeric in toluene, so that photoexcitation of ZnTPP results in
the charge separation reaction sequence: <sup>1*</sup>ZnTPP-PI-PDI
→ ZnTPP<sup>•+</sup>-PI<sup>•–</sup>-PDI
→ ZnTPP<sup>•+</sup>-PI-PDI<sup>•–</sup>, where τ<sub>CS1</sub> = 33 ps and τ<sub>CS2</sub> =
239 ps. The perpendicular orientation of ZnTPnP and ZnTPP relative
to PDI in <b>1</b> and <b>2</b> is designed to decrease
the porphyrin–PDI distance without greatly decreasing the overall
number of bonds linking them. This serves to decrease the Coulomb
energy penalty required to produce D<sup>•+</sup>-PI-PDI<sup>•–</sup> relative to the corresponding linear D-PI-PDI
array, while retaining the weak electronic coupling necessary to achieve
long-lived charge separation, as evidenced by τ<sub>CR</sub> = 24 ns for ZnTPP<sup>•+</sup>-PI-PDI<sup>•–</sup>
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