СОЗДАНИЕ БАЗЫ ДАННЫХ СЕЛЬСКОХОЗЯЙСТВЕННОЙ ТЕХНИКИ

Developed database for optimal selection of agricultural machinery. An analytical review of today’s database management systems (DBMS) has shown that an easy-to-use, easy-to-implement DBMS for various software solutions is SBMS ite. Entities have been created in the relational database: land plots, agricultural machines, aggregates. Tables reflecting the traction and coupling properties of modern foreign and domestic tractors and units have been created using DBMSite. The essence of land plots is described by a set of geometric and attributive properties reflecting spatial (area, perimeter) and also technological properties of land plots. Such properties include: the length of the site gon, the soil energy intensity score, soil resistivity, soil rockiness, relief angle, relief inclination coefficient, internal distance, road group coefficient, relief inclination coefficient along the route. Practical implementation was carried out on the example of the economy of the Mirny Kochenevsky district of the Novosibirsk region using the geographic information system ArcGIS 10.6. The developed database allows the selection of agricultural machinery and can be used to compare the selection of different versions of machine-tractor units taking into account the technological properties of land plots.Methods: database design methods.Results: A database of agricultural machinery has been developed with the help of SOUNDite, containing tables of agricultural machinery (tractors and aggregates) and land plots. Information on land plots is structured in the spatial database of geodata ArcGIS 10 and includes information on the area, type of use, as well as technological properties associated with the traction and coupling properties of tractors.Application of the results: the developed database of agricultural machinery will allow further calculation of time costs in soil cultivation on a specific land plot.Разработана база данных сельскохозяйственной техники. Выполненный аналитический обзор современных систем управления базами данных (СУБД) показал, что доступной в использовании, легко внедряемой в различные программные решения является СУБД SQLite. В реляционной базе данных созданы сущности: земельные участки, сельскохозяйственные машины, агрегаты. С использованием СУБД SQLite были созданы таблицы, отражающие тяглово-сцепные свойства современных зарубежных и отечественных тракторов и агрегатов. Сущность земельные участки описана набором геометрических и атрибутивных свойств, отражающих пространственные (площадь, периметр) и также технологические свойства земельных участков. К таким свойствам отнесены: длина гона участка, балл энергоемкости почв, удельное сопротивление почв, каменистость почв, угол наклона рельефа, коэффициент наклона рельефа, внутрихозяйственная удаленность, коэффициент группы дорог, коэффициент наклона рельефа по маршруту следования. Практическая реализация выполнена на примере хозяйства Мирный Коченевского района Новосибирской области с использованием геоинформационной системы ArcGIS 10.6. Разработанная база данных позволяет осуществлять выбор сельскохозяйственной техники и может быть использована для сравнительного анализа подбора разных вариантов машинно-тракторных агрегатов с учетом технологических свойств земельных участков.Цель: разработка базы данных для подбора сельскохозяйственной техники с учетом технологических свойств земельных участков.Методы работы: методы проектирования баз данных.Результаты: разработана база данных сельскохозяйственной техники с помощью SQLite, содержащая таблицы сельскохозяйственной техники (тракторов и агрегатов) и земельные участки. Информация о земельных участках структурирована в пространственной базе геоданных ArcGIS 10 и включает сведения о площади, типе использования, а также технологических свойствах, связанные с тяглово-сцепными свойствами тракторов.Область применения результатов: разработанная база данных сельскохозяйственной техники позволит в дальнейшем осуществлять расчет временных затрат при обработке почвы на конкретном земельном участке

Counter-Pandemic Vector of Remote Learning for University Students: Risks and Benefits of Educational Process Large-Scale Digitalization

The relevance of this study is due to the challenges of the global pandemic, which has led to the large-scale development of education digitalization. In this regard, this article is devoted to revealing the features of remote learning in the context of a global pandemic, identifying the risks and benefits of digital learning. The article reveals the discursive content of the counter-pandemic vector of distance learning for University students; defines the classification of risks and advantages of large-scale digitalization of students’ remote learning. Based on the results of the study, the authors justified the consolidated model of students distance learning, the counter-pandemic vector of which is a large-scale digitalization of the University educational process. The effectiveness of the model is proved by the results of its use in the process of large-scale digitalization of students’ remote learning in the context of a global pandemic. The materials of the article have practical application and can be useful in the development and implementation of digital educational complexes. They are recommended to teachers and students of the University, methodologists, curators, Tutors

Counter-Pandemic Vector of Remote Learning for University Students: Risks and Benefits of Educational Process Large-Scale Digitalization

The relevance of this study is due to the challenges of the global pandemic, which has led to the large-scale development of education digitalization. In this regard, this article is devoted to revealing the features of remote learning in the context of a global pandemic, identifying the risks and benefits of digital learning. The article reveals the discursive content of the counter-pandemic vector of distance learning for University students; defines the classification of risks and advantages of large-scale digitalization of students’ remote learning. Based on the results of the study, the authors justified the consolidated model of students distance learning, the counter-pandemic vector of which is a large-scale digitalization of the University educational process. The effectiveness of the model is proved by the results of its use in the process of large-scale digitalization of students’ remote learning in the context of a global pandemic. The materials of the article have practical application and can be useful in the development and implementation of digital educational complexes. They are recommended to teachers and students of the University, methodologists, curators, Tutors.La relevancia de este estudio se debe a los desafíos de la pandemia global, que ha llevado al desarrollo a gran escala de la digitalización de la educación. En este sentido, este artículo está dedicado a revelar las características del aprendizaje remoto en el contexto de una pandemia global, identificando los riesgos y beneficios del aprendizaje digital. El artículo revela el contenido discursivo del vector antipandémico del aprendizaje a distancia para estudiantes universitarios; define la clasificación de riesgos y ventajas de la digitalización a gran escala del aprendizaje remoto de los estudiantes. A partir de los resultados del estudio, los autores justificaron el modelo consolidado de educación a distancia de los estudiantes, cuyo vector contrapandémico es la digitalización a gran escala del proceso educativo universitario. La eficacia del modelo queda demostrada por los resultados de su uso en el proceso de digitalización a gran escala del aprendizaje remoto de los estudiantes en el contexto de una pandemia mundial. Los materiales del artículo tienen aplicación práctica y pueden ser útiles en el desarrollo e implementación de complejos educativos digitales. Se recomiendan a profesores y estudiantes de la Universidad, metodólogos, curadores, Tutores

Synthesis and Characterization of Cytidine Derivatives that Inhibit the Kinase IspE of the Non-Mevalonate Pathway for Isoprenoid Biosynthesis

The enzymes of the non-mevalonate pathway for isoprenoid biosynthesis are attractive targets for the development of novel drugs against malaria and tuberculosis. This pathway is used exclusively by the corresponding pathogens, but not by humans. A series of water-soluble, cytidine-based inhibitors that were originally designed for the fourth enzyme in the pathway, IspD, were shown to inhibit the subsequent enzyme, the kinase IspE (from Escherichia coli). The binding mode of the inhibitors was verified by co-crystal structure analysis, using Aquifex aeolicus IspE. The crystal structures represent the first reported example of a co-crystal structure of IspE with a synthetic ligand and confirmed that ligand binding affinity originates mainly from the interactions of the nucleobase moiety in the cytidine binding pocket of the enzyme. In contrast, the appended benzimidazole moieties of the ligands adopt various orientations in the active site and establish only poor intermolecular contacts with the protein. Defined binding sites for sulfate ions and glycerol molecules, components in the crystallization buffer, near the well-conserved ATP-binding Gly-rich loop of IspE were observed. The crystal structures of A. aeolicus IspE nicely complement the one from E. coli IspE for use in structure-based design, namely by providing invaluable structural information for the design of inhibitors targeting IspE from Mycobacterium tuberculosis and Plasmodium falciparum. Similar to the enzymes from these pathogens, A. aeolicus IspE directs the OH group of a tyrosine residue into a pocket in the active site. In the E. coli enzyme, on the other hand, this pocket is lined by phenylalanine and has a more pronounced hydrophobic character.

Lineage Conversion in Pediatric B-Cell Precursor Acute Leukemia under Blinatumomab Therapy

We report incidence and deep molecular characteristics of lineage switch in 182 pediatric patients affected by B-cell precursor acute lymphoblastic leukemia (BCP-ALL), who were treated with blinatumomab. We documented six cases of lineage switch that occurred after or during blinatumomab exposure. Therefore, lineage conversion was found in 17.4% of all resistance cases (4/27) and 3.2% of relapses (2/63). Half of patients switched completely from BCP-ALL to CD19-negative acute myeloid leukemia, others retained CD19-positive B-blasts and acquired an additional CD19-negative blast population: myeloid or unclassifiable. Five patients had KMT2A gene rearrangements; one had TCF3::ZNF384 translocation. The presented cases showed consistency of gene rearrangements and fusion transcripts across initially diagnosed leukemia and lineage switch. In two of six patients, the clonal architecture assessed by IG/TR gene rearrangements was stable, while in others, loss of clones or gain of new clones was noted. KMT2A-r patients demonstrated very few additional mutations, while in the TCF3::ZNF384 case, lineage switch was accompanied by a large set of additional mutations. The immunophenotype of an existing leukemia sometimes changes via different mechanisms and with different additional molecular changes. Careful investigation of all BM compartments together with all molecular –minimal residual disease studies can lead to reliable identification of lineage switch

Lineage Conversion in Pediatric B-Cell Precursor Acute Leukemia under Blinatumomab Therapy

We report incidence and deep molecular characteristics of lineage switch in 182 pediatric patients affected by B-cell precursor acute lymphoblastic leukemia (BCP-ALL), who were treated with blinatumomab. We documented six cases of lineage switch that occurred after or during blinatumomab exposure. Therefore, lineage conversion was found in 17.4% of all resistance cases (4/27) and 3.2% of relapses (2/63). Half of patients switched completely from BCP-ALL to CD19-negative acute myeloid leukemia, others retained CD19-positive B-blasts and acquired an additional CD19-negative blast population: myeloid or unclassifiable. Five patients had KMT2A gene rearrangements; one had TCF3::ZNF384 translocation. The presented cases showed consistency of gene rearrangements and fusion transcripts across initially diagnosed leukemia and lineage switch. In two of six patients, the clonal architecture assessed by IG/TR gene rearrangements was stable, while in others, loss of clones or gain of new clones was noted. KMT2A-r patients demonstrated very few additional mutations, while in the TCF3::ZNF384 case, lineage switch was accompanied by a large set of additional mutations. The immunophenotype of an existing leukemia sometimes changes via different mechanisms and with different additional molecular changes. Careful investigation of all BM compartments together with all molecular –minimal residual disease studies can lead to reliable identification of lineage switch