The impact of plant growth promoting bacteria (PGPB) on the development of phytopathogenic fungi

The main purpose of this study was to evaluate impact of plant growth promoting bacteria (PGPB) on the development of phytopathogenic fungi and correlate it with a potential effects on the growth of plants under unfavorable conditions, in order to improve the efficiency of a phytoremediation process. The conducted research focused on the antifungal properties of PGPB. In this study, 51 isolates of bacteria were obtained after diversified disinfection time from plants growing on soil after sewage sludge amendment. The results revealed that some isolated bacteria, mainly endophytic ones, inhibited the development of Fusarium oxysporum, F. culmorum and Alternaria alternata.Wyniki eksperymentu wskazują, że bakterie izolowane po czasie 2.5 i 10 minut sterylizacji, czyli głównie bakterie endofityczne, hamowały rozwój badanych grzybów - potencjalnych patogenów roślin. Badania wykazały także, że z rzodkiewnika pospolitego (Arabidopsis thaliana), znanego halofita, pozyskano najwyższą liczbę mikroorganizmów, które także wykazywały najlepsze właściwości inhibitujące wzrost grzybni wszystkich badanych patogenów

Effects of biochar addition on vermicomposting of food industry sewage sludge

Sewage sludge (SS) is a byproduct of wastewater treatment which is commonly used as fertilizer in the world. However, due to the possible contamination with heavy metals, xenobiotics and fecal pathogens, its application on fields is not so common in Poland. A safer alternative for agricultural use is the SS produced by food industry in small "inhouse" wastewater treatment plants, as substances that are used in its production are usually less harmful. As pretreatment of industrial wastewater is required before dumping the wastewater into common stream, the SS is an abundant byproduct that needs to be managed in an environmentally friendly and cost effective manner. Because the water content in SS is usually high, the dosage and logistics are problematic and thus we propose converting the sewage sludge into solid granular fertilizer in the vermicomposting process. Not only are the weight and volume of product decreased as a result, but also the nutrients such as N, P, and K become concentrated and made more accessible for plants. The SS is also further stabilized and less prone to produce odors and becoming putrid. The aim of this study was the in-depth analysis of the SS process vermicomposting with biochar. The SS was acquired from a local soft drink factory wastewater treatment plant. The batches of SS were inoculated with 20% mature vermicompost and E. fetida worms. Instead of typical bulking agents (like woodchips or straw) powdered biochar was used in the concentrations of 5, 10 and 15% as it exhibits beneficial influence on the process and increase the value of the final product. © 2019, Journal of Ecological Engineering.Polish Ministry of Science and Higher EducationMinistry of Science and Higher Education, Poland [BS/PB -401-304/11

Management of poultry manure in Poland : current state and future perspectives

This review aimed to analyse the current state of management practices for poultry manure in Poland and present future perspectives in terms of technologies allowing closing the loops for circular economy, and thus recovery of nutrients and energy. The scope of the review focused primarily on: (1) the analysis of poultry production and generation of poultry manure with special references to quantities, properties (e.g. fertilizing properties), seasonality, etc.; (2) the overview of current practices and methods for managing poultry manure including advantages and limitations; (3) the analysis of potential and realistic threats and risk related to managing poultry manure, and also (4) the analysis of promising technologies for converting poultry manure into added value products and energy. The review addressed the following technologies: composting of poultry manure to obtain fertilizers and soil improvers, anaerobic digestion of poultry manure for energy recovery, and also pyrolysis of poultry manure into different types of biochar that can be applied in agriculture, horticulture and industry. Poultry manure is rich in macro- and micronutrients but also can contain various contaminants such as antibiotics or pesticides, and thus posing a realistic threat to soil and living organisms when applied to soil directly or after biological treatment. The main challenge in poultry manure processing is to assure sufficient closing of carbon, nitrogen and phosphorous loops and safe application to soil

Potential and mechanisms for stable C storage in the post-mining soils under long-term study in mitigation of climate change

Carbon storage in soil increases along with remediation of post-mining soils. Despite many studies on the issue of carbon sequestration in soils, there is a knowledge gap in the potential and mechanisms of C sequestration in post-mining areas. This research, including nuclear magnetic resonance analysis, determines the soil organic carbon formation progress in a long-term study of limestone (S1), and lignite (S2) post-mining soil under different remediation stages. The main remediation target is reforesting; however, S2 was previously amended with sewage sludge. The study showed that for S1, the O-alkyl groups were the dominant fraction in sequestered soil. However, for S2, increased fractions of acetyl-C and aromatic C groups within remediation progress were observed. The remediation of S1 resulted in improved hydrophobicity and humification; however, the decrease in aromatic groups' formation and C/N ratio was noted. For S2, we noticed an increase for all indicators for sequestered C stability, which has been assigned to the used sewage sludge in remediation techniques. While both post-mining soils showed huge potential for C sequestration, S2 showed much higher properties of sequestered C indicating its higher stabilization which can suggest that soils non-amended with sewage sludge (S1) require more time for stable storage of C.Web of Science1422art. no. 761

Carbon sequestration in remediated post-mining soils: A new indicator for the vertical soil organic carbon variability evaluation in remediated post-mining soils

The present study experimentally investigated two different open-cast post-mining areas with different remediation methods for the vertical distribution of sequestered soil organic carbon (SOC). The study has been performed for two soil layers (0–15 cm, and 15–30 cm) for the four areas with different remediation advancement (up to 20 years) at both studied post-mining soils: the limestone post-mining soil remediated with embankment and lignite post-mining soil remediated with sewage sludge. The study revealed that SOC is more stable within soil depths for lignite post-mining soil remediated with sewage sludge in comparison to the limestone post-mining soil remediated with embankment. The lignite post-mining soil remediated with sewage sludge showed a better hydrophobicity, humidity, aromaticity, and C/N ratio according to the 13C NMR. Therefore, in that soil, an increased microbial community has been observed. The study observed a positive correlation between GRSP content with a fungi community within soil depths. For lignite post-mining soil remediated with sewage sludge, the activity of ureases and dehydrogenases was generally lower compared to the post-mining soil remediation with embankment. The investigation found good parameters of Ce and NCER which for both studied areas were negative which indicate for the privilege of the higher capturing of CO2 over its release from the soil into the atmosphere. The study finds no relevant changes in SOC, POXC, TC, and LOI content within soil depth and remediation age. Due to the lack of a possible well-describing indicator of the vertical distribution of SOC stability in post-mining remediation soil, we proposed two different indicators for differentially managed post-mining soil remediations. The model of calculation of vertical SOC variability index can be universally used for different post-mining soils under remediation, however, both proposed calculated indexes are unique for studied soils. The proposed model of an index may be helpful for remediation management, C sequestration prediction, and lowering the carbon footprint of mining activity.Web of Science1616art. no. 587

Bionor sewage sludge technology - Biomass to fertiliser and a soil addition

Wastewater treatment plants play a key role in preserving the resources of the environment, as well as human health. The applied technological system of a wastewater treatment plant determines the efficiency of wastewater and sewage sludge treatment, but also energy efficiency and operating costs. The aim of the presented work is to analyse the technological unconventional system of a small wastewater treatment plant with some modifications generating ecological profits, mainly the fertiliser, biomass or peat substitution ("biosolids factory" concept). The modification of the technological system consists in the application of sewage microfiltration, conditioning of sewage and sludge with cellulose material and application of short-term thermo-composting (Bionor technology). The use of a belt filter for suspension separation reduces the load on activated sludge bioreactors and aerobic stabilisation. The basic modification is the use of thermo-composting of separated sewage sludge with the addition of a structure-forming agent (straw pellet). Importantly, no precipitating or flocculating chemical substances or agents are used in this technology. The technology used enables the processing of sewage sludge into biomass with high fertilising and energy potential. In the following work, the Life Cycle Assessment (LCA) of a wastewater (and sewage sludge) treatment plants was made, comparing different possible concepts of sewage sludge management. The LCA was performed on five applied sewage sludge treatment scenarios: incineration of biomass (S1); biomass as a fertiliser product - "fertiliser substitution" (S2); biomass as a soil addition - "peat substitution" (S3); biomass as both fertiliser and a soil addition - "fertiliser and peat substitution" (S4); and biomass as a filling without any substitution benefits - "no specific use" (S5). The LCA analyses of the Bionor sludge technology applied in the wastewater and sewage sludge treatment plant confirm the environmental benefits and minimise the environmental impact with conventional solutions. The fertiliser and peat substitution scenario cause the least impact on climate change. This scenario was found as beneficial for avoiding net CO2 emissions (-5.8 kg CO2 eq/1 m(3) wastewater) and eutrophication potential (-1.7 to -2.0 kg N2O eq/1 m 3 wastewater, -2.78 to -3.0 PO4 eq/1 m(3) wastewater). The eco-toxicity study also showed low impact potentials for a land application scenario. Incineration of biomass yields higher environmental impacts than any application scenario (13.8 kg CO2 eq/1 m(3) wastewater). The LCA analysis confirmed that the peat substitution scenario was found as the most valuable.Web of Science319art. no. 12865

Anterior abdominal fixation — a new option in the surgical treatment of pelvic organ prolapse

Objectives: To present anterior abdominal fixation — a new surgical technique for the treatment of pelvic organ prolaps (POP) and to evaluate the results of the treatment of patients with stage III and IV POP operated using this technique.Material and methods: Anterior abdominal fixation for treating stage III and IV POP was carried out in 42 women, who were qualified according to the Pelvic Organ Prolapse Quantification System (POP-Q) scale at baseline and after 12 months. The Pelvic Floor Disability Index-20 (PFDI-20), along with its symptom scales, were evaluated.Results: The mean age 42 operated women was 64.5 years, and the average BMI was 27.3 (83% women were overweight). At baseline, 29 (69%) women had POP stage IV, and 13 (31%) women had POP stage III. Overall, 14 (33%) underwent laparoscopy, 28 (67%) underwent laparotomy. At 12 months, 14 (33.3%) women had POP stage I; 21 (50%) women had POP stage II. Seven patients (16.6%) experienced a recurrence of disease with advancement at the degree of III/IV; 4 (9.5%) women required adjuvant surgery in the form of anterior and posterior vaginal wall surgery. No early complications after surgery were observed. The comparison of the results before and after surgery showed statistically significant improvement in terms of the P-QoL score as well as PFDI-20 along with its 3 symptom scales.Conclusions: Anterior abdominal fixation of the uterus to the anterior abdominal wall is effective, safe, and technically easy to perform in the treatment of POP of advanced stage

The Role of Organic and Inorganic Amendments in Carbon Sequestration and Immobilization of Heavy Metals in Degraded Soils

To investigate the effect of organic and inorganic amendments on heavy metal immobilization in soil and organic carbon sequestration, a growth chamber study over a period of 18 months was conducted. Phytoremediation of two degraded soils, smelter-polluted soil and post-mining soil, was carried out using Scots Pine (Pinus Sylvestris L.) and Giant Miscanthus (Miscanthus x Giganteus). The increase in organic carbon content for lake chalk amended soils was noted. In addition, the largest root biomass production after fertilization of heavy metal contaminated soil with lake chalk was observed. This soil additive increases soil pH and reduces heavy metal mobility, which finally minimizes the harmful effect of metals on plant life and growth. Root biomass production of Giant Miscanthus was significantly higher than Scots Pine root biomass for both soils. This may indicate better phytosequestration properties of Giant Miscanthus, especially in the case of land degradation due to mining and destruction of soil profiles

The Chemophytostabilisation Process of Heavy Metal Polluted Soil.

Industrial areas are characterised by soil degradation processes that are related primarily to the deposition of heavy metals. Areas contaminated with metals are a serious source of risk due to secondary pollutant emissions and metal leaching and migration in the soil profile and into the groundwater. Consequently, the optimal solution for these areas is to apply methods of remediation that create conditions for the restoration of plant cover and ensure the protection of groundwater against pollution. Remediation activities that are applied to large-scale areas contaminated with heavy metals should mainly focus on decreasing the degree of metal mobility in the soil profile and metal bioavailability to levels that are not phytotoxic. Chemophytostabilisation is a process in which soil amendments and plants are used to immobilise metals. The main objective of this research was to investigate the effects of different doses of organic amendments (after aerobic sewage sludge digestion in the food industry) and inorganic amendments (lime, superphosphate, and potassium phosphate) on changes in the metals fractions in soils contaminated with Cd, Pb and Zn during phytostabilisation. In this study, the contaminated soil was amended with sewage sludge and inorganic amendments and seeded with grass (tall fescue) to increase the degree of immobilisation of the studied metals. The contaminated soil was collected from the area surrounding a zinc smelter in the Silesia region of Poland (pH 5.5, Cd 12 mg kg-1, Pb 1100 mg kg-1, Zn 700 mg kg-1). A plant growth experiment was conducted in a growth chamber for 5 months. Before and after plant growth, soil subsamples were subjected to chemical and physical analyses. To determine the fractions of the elements, a sequential extraction method was used according to Zeien and Brümmer. Research confirmed that the most important impacts on the Zn, Cd and Pb fractions included the combined application of sewage sludge from the food industry and the addition of lime and potassium phosphate. Certain doses of inorganic additives decreased the easily exchangeable fraction from 50% to 1%. The addition of sewage sludge caused a decrease in fraction I for Cd and Pb. In combination with the use of inorganic additives, a mobile fraction was not detected and an easily mobilisable fraction was reduced by half. For certain combinations of metals, the concentrations were detected up to a few percent. The application of sewage sludge resulted in a slight decrease in a mobile (water soluble and easily exchangeable metals) fraction of Zn, but when inorganic additives were applied, this fraction was not detected. The highest degree of immobilisation of the tested heavy metals relative to the control was achieved when using both sewage sludge and inorganic additives at an experimentally determined dose. The sequential extraction results confirmed this result. In addition, the results proved that the use of the phytostabilisation process on contaminated soils should be supported