Polymorphism and epitope sharing between the alleles of merozoite surface protein-1 of Plasmodium falciparum among Indian isolates

<p>Abstract</p> <p>Background</p> <p>The C-terminal region of merozoite surface protein-1 (MSP-1) is one of the leading candidates for vaccination against the erythrocytic stages of malaria. However, a major concern in the development of MSP-1 based malaria vaccine is the polymorphism observed in different geographical <it>Plasmodium falciparum </it>isolates. To explore whether the sequence heterogeneity of PfMSP-1 leads to variation in naturally acquired anti-MSP-1₁₉antibodies, the present study was undertaken to study PfMSP-1₁₉sequence polymorphism in malaria-endemic villages in eastern India and also carried out a competition enzyme-linked immunosorbent assay using three PfMSP-1₁₉variant forms.</p> <p>Methods</p> <p>The sequence variations in the C-terminal region of PfMSP-1₁₉were determined in a malaria endemic region. Three PfMSP-1₁₉variants were produced in <it>Escherichia coli </it>(PfMSP1₁₉QKNG-L, PfMSP1₁₉EKNG-L and PfMSP1₁₉ETSR-F) and an immunodepletion assay was carried out using the corresponding patients' sera.</p> <p>Results</p> <p>Results revealed predominance of PfMAD20 allele among Indian field isolates. Seven PfMSP-1₁₉variant forms were isolated in a singe geographical location. Three of PfMSP-1₁₉variant forms when expressed in <it>E. coli </it>showed presence of cross-reaction as well as variant specific antibodies in malaria infected patient sera.</p> <p>Conclusion</p> <p>The present study demonstrates the existence of allele specific antibodies in <it>P. falciparum</it>-infected patient sera, however their role in protection requires further investigation. These results thereby, suggest the importance of a multi-allelic PfMSP-1₁₉based vaccine for an effective malaria control.</p

SYNTHESIS AND EVALUATION OF DIAMINO SUBSTITUTED 1,3,4 THIADIAZOLE AS POSSIBLE BOMBYX MORI GROWTH ENHANCER

Sericulture or silk farming is the rearing of silkworms for the production of raw silk. The mulberry silkworm, Bombyx mori, is a domesticated and monophagous insect which feeds only on the leaves of mulberry for its nutrition. Thiadiazoles are heterocyclic compounds containing oxygen and nitrogen. 1, 3, 4, thiadiazoles are extensively studied and are known to play diverse biological activities. Diamines or polyamines are found in various biological fluids and are necessary for optimal growth, replication and metabolism of every cell in the body. This report describes the synthesis of diamino substituted thiadiazole and its effect on the growth parameters of Bombyx mori silk worm. Results show that the title compound at high concentration resulted in increased worm weight, silk gland weight and cocoon weight and at low concentrations proved to be beneficial for improving the pupal weight. We conclude that the synthesized compound can be fed to B. mori silk worms for improving their economic parameters

Sequence polymorphisms in the receptor-binding domain of Plasmodium falciparum EBA-175: implications for malaria vaccine development

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Immunodepletion assay showing the presence of PfMSP1allele specific antibodies in sera from infected patients

<p><b>Copyright information:</b></p><p>Taken from "Polymorphism and epitope sharing between the alleles of merozoite surface protein-1 of among Indian isolates"</p><p>http://www.malariajournal.com/content/6/1/95</p><p>Malaria Journal 2007;6():95-95.</p><p>Published online 20 Jul 2007</p><p>PMCID:PMC1950510.</p><p></p> Error bars indicate S.D