FORMULASINANOPARTIKELKITOSAN-PGV~ DENGAN METODEIONIKGELASI

Pentagamavllnon-O (PGV-O) merupakan salah satu senyawa sintetik turunan kurkumin. Aktivitas antiinflamasi dan antikanker PGV-O terbukti lebih besar dibanding kurkumin. Namun, PGV-O tidak larut dalam air sehingga mempengaruhi bioavaibilitasnya. Oleh karena itu, PGV-0 dimodifikasi menjadi nanopartikel yang menjadi salah satu teknologi peningkatan bioavaibilitas. Nanopartikel kitosan-PGV-O dapat dibuat dengan metode ionik gelasi menggunakan natrium tripilifosfat (TPP). Penelitian ini bbertujuan untuk mengasilkan nanopartikel kitosan-PGV-O, mengetahui profil pelepasan obat secara in vitro dan mengetahui pengaruh konsentrasi kitosan terhadap distribusi ukuran partikel dan entrapment efficiency nanopartikel kitosan-PGV-O. Kitosan dilarutkan dalam larutan dapar asetat pada pH 4,0 dengan berbagai variasi konsentrasi (0,02%0,06%0,10%) sedangkan PGV-O dilarutkan dalam aseton. Konsentrasi natrium triploifosfat yang digunakan yaitu 6 % dalam akuades. Karakterisasi nanopartikel kitosan-PGV-O dilakukan dengan pengukuran entrapment efficiency, pelepasan obat, distribusi ukuran partikel dan karakterisasi spektra inframerah. Dari penelitian ini, formulasi nanopartikel menghasilkan kompleks nanopartikel kitosan-PGV-O dengan ukuran 151,2 nm hingga 289,0 nm. Entrapment efficiency yang diperoleh yaitu: 90.24 %:J: 1,79 (kitosan 0,02%)90,41 %:J:0,98 (kitosan 0,06%)dan 85,09%:J: 1,19 (kitosan 0,10%). Profif pelepasan nanopartikel kitosan-PGV-O secara in vitro menunjukkan pelepasan obat secara cepat diikuti pelepasan secara perlahan. Profil ini menyerupai profil pelepasan obat terkontrol (sustained release). Dapat disimpulkan bahwa formulasi ini dapat menghasilkan nanopartikel, memiliki entrapment efficiency tinggi dan dapat digunakan sebagai pertimbangan bentuk sediaan obat terkontrol (sustained release)

Endothelial Nitric Oxide Synthase (eNOS) and the Cardiovascular System: In Physiology and in Disease States

Endothelial nitric oxide synthase (eNOS) plays a critical role in regulating and maintaining a healthy cardiovascular system. The importance of eNOS can be emphasized from the genetic polymorphisms of the eNOS gene, uncoupling of eNOS dimerization, and its numerous signaling regulations. The activity of eNOS on the cardiac myocytes, vasculature, and the central nervous system are discussed. The effects of eNOS on the sympathetic autonomic nervous system (SANS) and the parasympathetic autonomic nervous system (PANS), both of which profoundly influence the cardiovascular system, will be elaborated. The relationship between the eNOS protein with cardiovascular autonomic reflexes such as the baroreflex and the Exercise Pressor Reflex will be discussed. For example, the effects of endogenous nitric oxide (NO) are shown to be mediated by the eNOS protein and that eNOS-derived endothelial NO is most effective in regulating blood pressure oscillations via modulating the baroreflex mechanisms. The protective action of eNOS on the CVS is emphasized here because dysfunction of the eNOS enzyme is intricately correlated with the pathogenesis of several cardiovascular diseases such as hypertension, arteriosclerosis, myocardial infarction, and stroke. Overall, our current understanding of the eNOS protein with a focus on its role in the modulation, regulation, and control of the cardiovascular system in a normal physiological state and in cardiovascular diseases are discussed

Sequence Variation in Malayan Tapir (Tapirus indicus) Inferred Using Partial Sequences of the Cytochrome b Segment of the Mitochondrial DNA

Comparison of 321 bp long mtDNA cytochrome b sequences of wild and captive Malayan tapir (Tapirus indicus)revealed low variation among the individuals investigated. Phylogenetic analyses using distance (neighbor-joining) analysis supported the monophyletic status of the Malayan tapir. Two haplotypes were identified out of 13 Malayan tapir analyzed

Comparative study of Ce0.80Sm0.20 Ba0.80Y0.20O3-δ (YB-SDC) electrolyte by various chemical synthesis routes

Solid Oxide Fuel Cells is received a significant attention in recent years due to higher efficiency and fuel flexibility. The one of the main challenge for SOFC is to lower the operating temperature of SOFCs. Therefore, different strategies are used in order to enhance the ionic conduction of electrolyte, which can lower the overall SOFC operating temperature. The present work is focused on this strategy to enhance the electrolytic conductivity. Therefore, the ceria based composite electrolytes Ce0.80Sm0.20B0.80Y0.20O3-δ (YBSDC) are synthesized using three different approaches i.e. co-precipitation (YBSDC-1), sol-gel (YBSDC-2) and ball milling (YBSDC-3). Their crystal structures and surface morphologies are characterized through X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) techniques, respectively. The four-probe technique is employed to measure their dc conductivities in the temperature range (300–700) °C under air atmosphere. The open circuit voltage (OCV) and current are recorded with natural gas as fuel {flow rate kept at 100 ml min−1 at 1 atm pressure} over the temperature range (300–600) °C.The electrolyte (YBSDC-1) prepared by co-precipitation technique is shown better results as compare to other two electrolytes (YBSDC-2 and YBSDC-3). The electrolyte (YBSDC-1) having maximum dc conductivity (0.096 S/cm), peak power density 224 mW cm−2 and OCV 0.94 V at 600 °C. These results show that YBSDC-1electrolyte is potential candidate for low temperature SOFCs. Keywords: Hydrogen, Energy, Ball milling, Composite, Conducto

Comparative study of Ce0.80Sm0.20Ba0.80Y0.20O3-delta (YB-SDC) electrolyte by various chemical synthesis routes

Solid Oxide Fuel Cells is received a significant attention in recent years due to higher efficiency and fuel flexibility. The one of the main challenge for SOFC is to lower the operating temperature of SOFCs. Therefore, different strategies are used in order to enhance the ionic conduction of electrolyte, which can lower the overall SOFC operating temperature. The present work is focused on this strategy to enhance the electrolytic conductivity. Therefore, the ceria based composite electrolytes Ce0.80Sm0.20B0.80Y0.20O3-delta (YBSDC) are synthesized using three different approaches i.e. co-precipitation (YBSDC-1), sol-gel (YBSDC-2) and ball milling (YBSDC-3). Their crystal structures and surface morphologies are characterized through X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) techniques, respectively. The four-probe technique is employed to measure their dc conductivities in the temperature range (300-700) degrees C under air atmosphere. The open circuit voltage (OCV) and current are recorded with natural gas as fuel {flow rate kept at 100 ml min(-1) at 1 atm pressure} over the temperature range (300-600) degrees C. The electrolyte (YBSDC-1) prepared by co-precipitation technique is shown better results as compare to other two electrolytes (YBSDC-2 and YBSDC-3). The electrolyte (YBSDC-1) having maximum dc conductivity (0.096 S/cm), peak power density 224 mW cm(-2) and OCV 0.94 V at 600 degrees C. These results show that YBSDC-1electrolyte is potential candidate for low temperature SOFCs. (C) 2018 Published by Elsevier B.V

HIV proviral genetic diversity, compartmentalization and inferred dynamics in lung and blood during long-term suppressive antiretroviral therapy.

The lung is an understudied site of HIV persistence. We isolated 898 subgenomic proviral sequences (nef) by single-genome approaches from blood and lung from nine individuals on long-term suppressive antiretroviral therapy (ART), and characterized genetic diversity and compartmentalization using formal tests. Consistent with clonal expansion as a driver of HIV persistence, identical sequences comprised between 8% to 86% of within-host datasets, though their location (blood vs. lung) followed no consistent pattern. The majority (77%) of participants harboured at least one sequence shared across blood and lung, supporting the migration of clonally-expanded cells between sites. The extent of blood proviral diversity on ART was also a strong indicator of diversity in lung (Spearman's ρ = 0.98, p<0.0001). For three participants, insufficient lung sequences were recovered to reliably investigate genetic compartmentalization. Of the remainder, only two participants showed statistically significant support for compartmentalization when analysis was restricted to distinct proviruses per site, and the extent of compartmentalization was modest in both cases. When all within-host sequences (including duplicates) were considered, the number of compartmentalized datasets increased to four. Thus, while a subset of individuals harbour somewhat distinctive proviral populations in blood and lung, this can simply be due to unequal distributions of clonally-expanded sequences. For two participants, on-ART proviruses were also phylogenetically analyzed in context of plasma HIV RNA populations sampled up to 18 years prior, including pre-ART and during previous treatment interruptions. In both participants, on-ART proviruses represented the most ancestral sequences sampled within-host, confirming that HIV sequences can persist in the body for decades. This analysis also revealed evidence of re-seeding of the reservoir during treatment interruptions. Results highlight the genetic complexity of proviruses persisting in lung and blood during ART, and the uniqueness of each individual's proviral composition. Personalized HIV remission and cure strategies may be needed to overcome these challenges

HIV-1 diversity considerations in the application of the Intact Proviral DNA Assay (IPDA).

The intact proviral DNA assay quantifies the genomically intact HIV reservoir, but assay failure due to HIV-1 polymorphism has been observed. Here, the authors report a 28% failure rate in a cohort of people with HIV-1, and note within-host HIV-1 diversity as a further challenge to IPDA accuracy

The replication-competent HIV reservoir is a genetically restricted, younger subset of the overall pool of HIV proviruses persisting during therapy, which is highly genetically stable over time

Within-host HIV populations continually diversify during untreated infection, and members of these diverse forms persist within infected cell reservoirs, even during antiretroviral therapy (ART). Characterizing the diverse viral sequences that persist during ART is critical to HIV cure efforts, but our knowledge of on-ART proviral evolutionary dynamics remains incomplete, as does our understanding of the differences between the overall pool of persisting proviral DNA (which is largely genetically defective) and the subset of intact HIV sequences capable of reactivating. Here, we reconstructed within-host HIV evolutionary histories in blood from seven participants of the Women’s Interagency HIV Study (WIHS) who experienced HIV seroconversion. We measured diversity, lineage origins and ages of proviral sequences ( env-gp120 ) sampled up to four times, up to 12 years on ART. We used the same techniques to study HIV sequences emerging from the reservoir in two participants. Proviral clonality generally increased over time on ART, with clones frequently persisting across multiple time points. The integration dates of proviruses persisting on ART generally spanned the duration of untreated infection (though were often skewed towards years immediately pre-ART), while in contrast, reservoir-origin viremia emerging in plasma was exclusively "younger" (i.e., dated to the years immediately pre-ART). The genetic and age distributions of distinct proviral sequences remained highly stable during ART in all but one participant in whom, after 12 years, there was evidence that "younger" proviruses had been preferentially eliminated. Analysis of within-host recombinant proviral sequences also suggested that HIV reservoirs can be superinfected with virus reactivated from an older era, yielding infectious viral progeny with mosaic genomes of sequences with different ages. Overall, results underscore the remarkable genetic stability of distinct proviral sequences that persist on ART, yet suggest that replication-competent HIV reservoir represents a genetically-restricted and overall "younger" subset of the overall persisting proviral pool in blood