25 research outputs found
Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscle
International audienceBACKGROUND: MicroRNAs (miRNAs) are small RNA molecules that post-transcriptionally regulate gene expression and have been shown to play an important role during development. miR-1, miR-133a, miR-133b and miR-206 are expressed in muscle tissue and induced during muscle cell differentiation, a process that directs myoblasts to differentiate into mature myotubes, which are organized into myofibers. Although miR-1, miR-133a, miR-133b and miR-206 are well-studied in muscle, there is no information about their expression and function during human development. The purpose of this study was to determine the profile of these miRNAs in muscle cells isolated from different stages of human development. RESULTS: We examined the levels of miR-1, miR-133a, miR-133b and miR-206 during the development of human foetus. All four miRNA levels were found increased during late stages of human foetal muscle development. Increases in the expression levels of these miRNAs were proportional to the capacity of myoblasts to form myotubes. Changes in miRNA levels during human foetal development were accompanied by endogenous alterations in their known targets and also in their inducer, MyoD. Ectopic MyoD expression caused an induction of muscle cell differentiation in vitro, accompanied by an increase in the levels of miR-1, miR-133a, miR-133b and miR-206. CONCLUSIONS: This study provides data about the profile of four miRNAs in human muscle cells isolated during different stages of foetal development. These results may shed light on the differentiation of muscle cells and regulation of muscle formation through miRNAs, during the development of human foetus
Investigation of transcriptional and post-transcriptional regulation of myogenesis
Skeletal myogenesis IS a complicated and tightly regulated process, at both transcriptional and post-transcriptional levels. Muscle cells isolated from different stages of the human foetal development displayed increased capacity to differentiate in vitro at late stages of the development. Twist is an early developmental transcription factor shown to inhibit muscle differentiation in mice. Endogenous human TWIST (H-TWIST) protein levels were found to be inversely proportional to the state of foetal muscle development and the capacity of isolated myoblasts to differentiate in vitro. Investigation of H-TWIST gene transcriptional regulation revealed that endogenous MyoD binds to the H- TWIST promoter and inhibits its expression. Overexpression of MyoD increased the low capacity of human foetal myoblasts to differentiation in vitro and decreased the levels of H- TWIST protein. These results propose a mechanism by which MyoD downregulates the expression of H-TWIST gene, thus promoting myogenesis. MicroRNAs are non-coding RNA molecules that post-transcriptionally regulate many cellular processes. MiR-l, miR- 133a, miR-133b and miR-206, also known as myomiRs, are expressed in muscle tissue and induced during muscle cell differentiation. MyomiRs were found to be increased during late stages of human foetal muscle development. Increases in their expression levels were proportional to the capacity of myoblasts to differentiate in vitro. Changes in myomiR levels during human foetal development were accompanied by endogenous alterations in their known targets and also in their inducer, MyoD. Overexpression of the latter resulted in an induction of muscle cell differentiation in vitro, accompanied by an increase in the levels of miR-l, miR-133a, miR-133b and miR-206. Myotonic dystrophy type 1 is a muscular dystrophy characterized by impaired muscle cell differentiation. Muscle cells isolated from DMl patients from distinct developmental stages showed a reduction in the expression levels of myomiRs during both differentiated and undifferentiated stages, verifying their important role during myogenesis.EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Muscle‐derived exosomes encapsulate myomiRs and are involved in local skeletal muscle tissue communication
Exosomes are extracellular vesicles that are released from most cell types encap-sulating specific molecular cargo. Exosomes serve as mediators of cell-to-cell and tissue-to-tissue communications under normal and pathological conditions. It has been shown that exosomes carrying muscle-specific miRNAs, myomiRs, are se-creted from skeletal muscle cells in vitro and are elevated in the blood of muscle disease patients. The aim of this study was to investigate the secretion of exosomes encapsulating the four myomiRs from skeletal muscle tissues and to assess their role in inter-tissue communication between neighboring skeletal muscles in vivo. We demonstrate, for the first time, that isolated, intact skeletal muscle tissues secrete exosomes encapsulating the four myomiRs, miR-1, miR-133a, miR-133b, and miR-206. Notably, we show that the sorting of the four myomiRs within exosomes var-ies between skeletal muscles of different muscle fiber-type composition. miR-133a and miR-133b downregulation in TA muscles caused a reduction of their levels in neighboring skeletal muscles and in serum exosomes. In conclusion, our results re-veal that skeletal muscle-derived exosomes encapsulate the four myomiRs, some of which enter the blood, while a portion is used for the local communication between proximal muscle tissues. These findings provide important evidence regarding novel pathways implicated in skeletal muscle function
Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
Skeletal muscle growth and maintenance depend on two tightly regulated processes, myogenesis and muscle regeneration. Both processes involve a series of crucial regulatory molecules including muscle-specific microRNAs, known as myomiRs. We recently showed that four myomiRs, miR-1, miR-133a, miR-133b, and miR-206, are encapsulated within muscle-derived exosomes and participate in local skeletal muscle communication. Although these four myomiRs have been extensively studied for their function in muscles, no information exists regarding their endogenous and exosomal levels across age. Here we aimed to identify any age-related changes in the endogenous and muscle-derived exosomal myomiR levels during acute skeletal muscle growth. The four endogenous and muscle-derived myomiRs were investigated in five skeletal muscles (extensor digitorum longus, soleus, tibialis anterior, gastrocnemius, and quadriceps) of 2-week-1-year-old wild-type male mice. The expression of miR-1, miR-133a, and miR-133b was found to increase rapidly until adolescence in all skeletal muscles, whereas during adulthood it remained relatively stable. By contrast, endogenous miR-206 levels were observed to decrease with age in all muscles, except for soleus. Differential expression of the four myomiRs is also inversely reflected on the production of two protein targets; serum response factor and connexin 43. Muscle-derived exosomal miR-1, miR-133a, and miR-133b levels were found to increase until the early adolescence, before reaching a plateau phase. Soleus was found to be the only skeletal muscle to release exosomes enriched in miR-206. In this study, we showed for the first time an in-depth longitudinal analysis of the endogenous and exosomal levels of the four myomiRs during skeletal muscle development. We showed that the endogenous expression and extracellular secretion of the four myomiRs are associated to the function and size of skeletal muscles as the mice age. Overall, our findings provide new insights for the myomiRs' significant role in the first year of life in mice
Elevated Muscle-Specific miRNAs in Serum of Myotonic Dystrophy Patients Relate to Muscle Disease Progress
The discovery of reliable and sensitive blood biomarkers is useful for
the diagnosis, monitoring and potential future therapy of diseases.
Recently, microRNAs (miRNAs) have been identified in blood circulation
and might have the potential to be used as biomarkers for several
diseases and clinical conditions. Myotonic Dystrophy type 1 (DM1) is the
most common form of adult-onset muscular dystrophy primarily
characterized by muscle myotonia, weakness and atrophy. Previous studies
have shown an association between miRNAs and DM1 in muscle tissue and,
recently, in plasma. The aim of this study was to detect and assess
muscle-specific miRNAs as potential biomarkers of DM1 muscle wasting, an
important parameter in the disease’s natural history. Disease stable or
progressive DM1 patients with muscle weakness and wasting were recruited
and enrolled in the study. RNA isolated from participants’ serum was
used to assess miRNA levels. Results suggest that the levels of
muscle-specific miRNAs are correlated with the progression of muscle
wasting and weakness observed in the DM1 patients. Specifically, miR-1,
miR-133a, miR133b and miR-206 serum levels were found elevated in DM1
patients with progressive muscle wasting compared to disease stable DM1
patients. Based on these results, we propose that muscle-specific miRNAs
might be useful molecular biomarkers for monitoring the progress of
muscle atrophy in DM1 patients
Elevated Muscle-Specific miRNAs in Serum of Myotonic Dystrophy Patients Relate to Muscle Disease Progress
<div><p>The discovery of reliable and sensitive blood biomarkers is useful for the diagnosis, monitoring and potential future therapy of diseases. Recently, microRNAs (miRNAs) have been identified in blood circulation and might have the potential to be used as biomarkers for several diseases and clinical conditions. Myotonic Dystrophy type 1 (DM1) is the most common form of adult-onset muscular dystrophy primarily characterized by muscle myotonia, weakness and atrophy. Previous studies have shown an association between miRNAs and DM1 in muscle tissue and, recently, in plasma. The aim of this study was to detect and assess muscle-specific miRNAs as potential biomarkers of DM1 muscle wasting, an important parameter in the disease’s natural history. Disease stable or progressive DM1 patients with muscle weakness and wasting were recruited and enrolled in the study. RNA isolated from participants’ serum was used to assess miRNA levels. Results suggest that the levels of muscle-specific miRNAs are correlated with the progression of muscle wasting and weakness observed in the DM1 patients. Specifically, miR-1, miR-133a, miR133b and miR-206 serum levels were found elevated in DM1 patients with progressive muscle wasting compared to disease stable DM1 patients. Based on these results, we propose that muscle-specific miRNAs might be useful molecular biomarkers for monitoring the progress of muscle atrophy in DM1 patients.</p></div
Statistical analysis of muscle-specific miRNA levels in the serum of moderate and not-moderate DM1 patients with non-progressive and progressive muscle wasting.
<p>Statistical analysis of muscle-specific miRNA levels in the serum of moderate and not-moderate DM1 patients with non-progressive and progressive muscle wasting.</p
Statistical analysis of muscle-specific miRNA levels in the serum of DM1 patients with non-progressive and progressive muscle wasting.
<p>Statistical analysis of muscle-specific miRNA levels in the serum of DM1 patients with non-progressive and progressive muscle wasting.</p