Surgical optimization and characterization of a minimally invasive aortic banding procedure to induce cardiac hypertrophy in mice

Left ventricular pressure overload in response to aortic banding is an invaluable model for studying progression of cardiac hypertrophy and transition to heart failure. Traditional aortic banding has recently been superceded by minimally invasive transverse aortic banding (MTAB), which does not require ventilation so is less technically challenging. Although the MTAB approach is superior, few laboratories have documented success, and minimal information on the model is available. The aim of this study was to optimize conditions for MTAB and to characterize the development and progression of cardiac hypertrophy. Isofluorane proved the most suitable anaesthetic for MTAB surgery in mice, and 1 week after surgery the MTAB animals showed significant increases in systolic blood pressure (MTAB 110 ± 6 mmHg versus sham 78 ± 3 mmHg, n = 7, P <0.0001) and heart weight to body weight ratio (MTAB 6.2 ± 0.2 versus sham 5.1 ± 0.1, n = 12, P <0.001), together with systolic (e.g. fractional shortening, MTAB 31.7 ± 1% versus sham 36.6 ± 1.4%, P = 0.01) and diastolic dysfunction (e.g. left ventricular end-diastolic pressure, MTAB 12.7 ± 1.0 mmHg versus sham 6.7 ± 0.8 mmHg, P <0.001). Leucocyte infiltration to the heart was evident after 1 week in MTAB hearts, signifying an inflammatory response. More pronounced remodelling was observed 4 weeks postsurgery (heart weight to body weight ratio, MTAB 9.1 ± 0.6 versus sham 4.6 ± 0.04, n = 10, P <0.0001) and fractional shortening was further decreased (MTAB 24.3 ± 2.5% versus sham 43.6 ± 1.7%, n = 10, P = 0.003), together with a significant increase in cardiac fibrosis and further cardiac inflammation. Our findings demonstrate that MTAB is a relevant experimental model for studying development and progression of cardiac hypertrophy, which will be highly valuable for future studies examining potential novel therapeutic interventions in this setting

Euclid: Nonparametric point spread function field recovery through interpolation on a graph Laplacian

Context. Future weak lensing surveys, such as the Euclid mission, will attempt to measure the shapes of billions of galaxies in order to derive cosmological information. These surveys will attain very low levels of statistical error, and systematic errors must be extremely well controlled. In particular, the point spread function (PSF) must be estimated using stars in the field, and recovered with high accuracy. Aims. The aims of this paper are twofold. Firstly, we took steps toward a nonparametric method to address the issue of recovering the PSF field, namely that of finding the correct PSF at the position of any galaxy in the field, applicable to Euclid. Our approach relies solely on the data, as opposed to parametric methods that make use of our knowledge of the instrument. Secondly, we studied the impact of imperfect PSF models on the shape measurement of galaxies themselves, and whether common assumptions about this impact hold true in an Euclid scenario. Methods. We extended the recently proposed resolved components analysis approach, which performs super-resolution on a field of under-sampled observations of a spatially varying, image-valued function. We added a spatial interpolation component to the method, making it a true 2-dimensional PSF model. We compared our approach to PSFEx, then quantified the impact of PSF recovery errors on galaxy shape measurements through image simulations. Results. Our approach yields an improvement over PSFEx in terms of the PSF model and on observed galaxy shape errors, though it is at present far from reaching the required Euclid accuracy. We also find that the usual formalism used for the propagation of PSF model errors to weak lensing quantities no longer holds in the case of an Euclid-like PSF. In particular, different shape measurement approaches can react differently to the same PSF modeling errors.This work was supported by the Centre National d'Etudes Spatiales and the European Community through the grant DEDALE (contract no. 665044) within the H2020 Framework Program. JB acknowledges support by Funda??o para a Ci?ncia e a Tecnologia through national funds (UID/FIS/04434/2013) and Investigador FCT contract IF/01654/2014/CP1215/CT0003, and by FEDER through COMPETE2020 (POCI-01-0145-FEDER-007672). The authors acknowledge the Euclid Consortium, the European Space Agency and the support of a number of agencies and institutes that have supported the development of Euclid. A detailed complete list is available on the Euclid web site (http://www. euclid-ec.org). In particular the Academy of Finland, the Agenzia Spaziale Italiana, the Belgian Science Policy, the Canadian Euclid Consortium, the Centre National d'Etudes Spatiales, the Deutsches Zentrum f?r Luft- and Raumfahrt, the Danish Space Research Institute, the Funda??o para a Ci?nca e a Tecnologia, the Ministerio de Economia y Competitividad, the National Aeronautics and Space Administration, the Netherlandse Onderzoekschool Voor Astronomie, the Norvegian Space Center, the Romanian Space Agency, the State Secretariat for Education, Research and Innovation (SERI) at the Swiss Space Offce (SSO), and the United Kingdom Space Agency. This work was supported by the Centre National d’Etudes Spatiales and the European Community through the grant DEDALE (contract no. 665044) within the H2020 Framework Program. JB acknowledges support by Fundação para a Ciên-cia e a Tecnologia through national funds (UID/FIS/04434/2013) and Investi-gador FCT contract IF/01654/2014/CP1215/CT0003, and by FEDER through COMPETE2020 (POCI-01-0145-FEDER-007672). The authors acknowledge the Euclid Consortium, the European Space Agency and the support of a number of agencies and institutes that have supported the development of Euclid. A detailed complete list is available on the Euclid web site (http://www. euclid-ec.org). In particular the Academy of Finland, the Agenzia Spaziale Italiana, the Belgian Science Policy, the Canadian Euclid Consortium, the Centre National d’Etudes Spatiales, the Deutsches Zentrum für Luft-and Raumfahrt, the Danish Space Research Institute, the Fundação para a Ciênca e a Tecnolo-gia, the Ministerio de Economia y Competitividad, the National Aeronautics and Space Administration, the Netherlandse Onderzoekschool Voor Astronomie, the Norvegian Space Center, the Romanian Space Agency, the State Secretariat for Education, Research and Innovation (SERI) at the Swiss Space Office (SSO), and the United Kingdom Space Agency