Transiently Transfected Purine Biosynthetic Enzymes Form Stress Bodies

It has been hypothesized that components of enzymatic pathways might organize into intracellular assemblies to improve their catalytic efficiency or lead to coordinate regulation. Accordingly, de novo purine biosynthesis enzymes may form a purinosome in the absence of purines, and a punctate intracellular body has been identified as the purinosome. We investigated the mechanism by which human de novo purine biosynthetic enzymes might be organized into purinosomes, especially under differing cellular conditions. Irregardless of the activity of bodies formed by endogenous enzymes, we demonstrate that intracellular bodies formed by transiently transfected, fluorescently tagged human purine biosynthesis proteins are best explained as protein aggregation.This work was supported by grants from the United States National Institutes of Health, National Science Foundation, and Welch (F1515) and Packard Foundations to EMM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Cellular and Molecular Biolog

Collisions of boosted black holes: perturbation theory prediction of gravitational radiation

We consider general relativistic Cauchy data representing two nonspinning, equal-mass black holes boosted toward each other. When the black holes are close enough to each other and their momentum is sufficiently high, an encompassing apparent horizon is present so the system can be viewed as a single, perturbed black hole. We employ gauge-invariant perturbation theory, and integrate the Zerilli equation to analyze these time-asymmetric data sets and compute gravitational wave forms and emitted energies. When coupled with a simple Newtonian analysis of the infall trajectory, we find striking agreement between the perturbation calculation of emitted energies and the results of fully general relativistic numerical simulations of time-symmetric initial data.Comment: 5 pages (RevTex 3.0 with 3 uuencoded figures), CRSR-107

The Star Formation History of LGS 3

We have determined the distance and star formation history of the Local Group dwarf galaxy LGS 3 from deep Hubble Space Telescope WFPC2 observations. LGS 3 is intriguing because ground-based observations showed that, while its stellar population is dominated by old, metal-poor stars, there is a handful of young, blue stars. Also, the presence of HI gas makes this a possible ``transition object'' between dwarf spheroidal and dwarf irregular galaxies. The HST data are deep enough to detect the horizontal branch and young main sequence for the first time. A new distance of D=620+/-20 kpc has been measured from the positions of the TRGB, the red clump, and the horizontal branch. The mean metallicity of the stars older than 8 Gyr is Fe/H = -1.5 +/- 0.3. The most recent generation of stars has Fe/H ~ -1. For the first few Gyr the global star formation rate was several times higher than the historical average and has been fairly constant since then. However, we do see significant changes in stellar populations and star formation history with radial position in the galaxy. Most of the young stars are found in the central 63 pc (21''), where the star formation rate has been relatively constant, while the outer parts have had a declining star formation rate.Comment: To appear in The Astrophysical Journal, 26 pages, 14 figures, uses AASTe

Genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of Medicago and chickpea.

Lentil (Lens culinaris Medik.) is a global food crop with increasing importance for food security in south Asia and other regions. Lens ervoides, a wild relative of cultivated lentil, is an important source of agronomic trait variation. Lens is a member of the galegoid clade of the Papilionoideae family, which includes other important dietary legumes such as chickpea (Cicer arietinum) and pea (Pisum sativum), and the sequenced model legume Medicago truncatula. Understanding the genetic structure of Lens spp. in relation to more fully sequenced legumes would allow leveraging of genomic resources. A set of 1107 TOG-based amplicons were identified in L. ervoides and a subset thereof used to design SNP markers for mapping. A map of L. ervoides consisting of 377 SNP markers spread across seven linkage groups was developed using a GoldenGate genotyping array and single SNP marker assays. Comparison with maps of M. truncatula and L. culinaris documented considerable shared synteny and led to the identification of a few major translocations and a major inversion that distinguish Lens from M. truncatula, as well as a translocation that distinguishes L. culinaris from L. ervoides. The identification of chromosome-level differences among Lens spp. will aid in the understanding of introgression of genes from L. ervoides into cultivated L. culinaris, furthering genetic research and breeding applications in lentil

Guidance of sentinel lymph node biopsy decisions in patients with T1-T2 melanoma using gene expression profiling.

AIM: Can gene expression profiling be used to identify patients with T1-T2 melanoma at low risk for sentinel lymph node (SLN) positivity? PATIENTS & METHODS: Bioinformatics modeling determined a population in which a 31-gene expression profile test predicted \u3c5% SLN positivity. Multicenter, prospectively-tested (n = 1421) and retrospective (n = 690) cohorts were used for validation and outcomes, respectively. RESULTS: Patients 55-64 years and ≥65 years with a class 1A (low-risk) profile had SLN positivity rates of 4.9% and 1.6%. Class 2B (high-risk) patients had SLN positivity rates of 30.8% and 11.9%. Melanoma-specific survival was 99.3% for patients ≥55 years with class 1A, T1-T2 tumors and 55.0% for class 2B, SLN-positive, T1-T2 tumors. CONCLUSION: The 31-gene expression profile test identifies patients who could potentially avoid SLN biopsy

Granulocyte macrophage colony-stimulating factor receptor α expression and its targeting in antigen-induced arthritis and inflammation

A Representative FACS plots showing Ly6G and Ly6C staining of CD45+ myeloid populations in the AIA knee joint. F4/80intSSchi eosinophils (Eos), F4/80+CD11c+MHCII+ Mo-DCs (R1), F4/80+CD11c-MHCII+ macrophages (Macs) (R2), F4/80+CD11c-MHCII- macrophages (R3), F4/80-CD11c+ MHCII+ cDCs, F4/80-CD11b+Ly6G+ neutrophils, which are also Ly6C+, and F4/80-CD11b+Ly6G-SScloLy6C+/- monocytes. B Representative FACS plots of CD45+ myeloid populations in the AIA knee joint showing Ly6G+ neutrophils are CD64- and F4/80+ macrophages/Mo-DCs are CD64+. (PDF 235 kb

Is salivary cortisol moderating the relationship between salivary testosterone and hand-grip strength in healthy men?

This study examined the moderating effect of cortisol (C) on the relationship between testosterone (T) and hand-grip strength (HGS) in healthy young men. Sixty-five males were monitored for salivary T, C and HGS before and 15 min after a short bout (5 × 6-s trials) of sprint cycling exercise. Sprint exercise promoted (p < .05) positive changes in T (6.1 ± 24.9%) and HGS (3.4 ± 7.5%), but a negative C response (−14.4 ± 33.1%). The T and C measures did not independently predict HGS, but a significant T × C interaction was found in relation to these outcomes. Further testing revealed that pre-test T and HGS were negatively associated (p < .05), but only in men with high C levels. The exercise changes in T and HGS were also negatively related in men with low C levels (p < .05), but no relationship was seen in men with high C levels. In summary, complex relationships between T and HGS emerged when considering C as a moderating variable. The pre-test combination of high C and low T levels favoured absolute HGS, whereas low pre-test C levels and a smaller T change were linked to larger HGS changes. These associations suggest that, in the current format, T is not necessarily anabolic to muscle strength in healthy young men. Such complexities could also explain some of the inconsistent T relationships with physical performance in lesser trained male populations