Quantification of Squalene and Lactic Acid in Hair Bulbs with Damaged Sheaths: Are They Metabolic Wastes in Alopecia?

Alopecia is a pathological and multifactorial condition characterised by an altered hair growth cycle and ascribed to different pathogenic causes. Cell energetic imbalances in hair follicles occurring in this disorder could lead to the production of some “metabolic wastes”, including squalene and lactic acid, which could be involved in the clinically observed sheath damage. The aim of this work was the extraction and analytical quantification of squalene and lactic acid from hair bulbs of subjects with clinical alopecia in comparison with controls, using HPLC-DAD and HPLC-MS techniques. The analytical quantification was performed after a preliminary observation through a polarised optical microscope to assess sheath damage and morphological alterations in the cases group. A significantly larger amount of squalene was quantified only in subjects affected by alopecia (n = 31) and with evident damage to hair sheaths. For lactic acid, no statistically significant differences were found between cases (n = 21) and controls (n = 21) under the experimental conditions used. Therefore, the obtained results suggest that squalene can represent a metabolic and a pathogenic marker for some alopecia conditions

Calving time identified by the automatic detection of tail movements and rumination time, and observation of cow behavioural changes

The use of electronic devices to improve animal health, welfare and farm efficiency in precision livestock farming is a developing area of great scientific and commercial interest. In particular, the use of on-site dairy farm instruments to detect calving is a tool in reproduction livestock farming. The primary aim of this study was to validate the ability of the Moocall device (MD) to detect calving cows. In addition, behavioural changes in parturient dairy cows were evaluated using video-based observations. The MD was applied approximately 9 days before cow delivery. Observational sessions were conducted three times a day for each cow from the day before MD application (CTR day) to calving time. The sensitivity and specificity at 3h and 24h before calving were measured to test the effectiveness of the MD. In addition, behavioural changes were investigated before and after the MD application as well as before and during calving time. The 3h sensitivity (Se) and the 3h specificity (Sp) obtained were 95.2% and 71.4%, respectively. No false negatives were observed in the 24h before delivery (24h Se =100%) while the 3h Se was 95.2%. The MD was well tolerated by the dairy cows since no change in behaviours was observed in this study among the cows with or without the MD, except for an increase in eating behaviour in the animals with the MD. As regards the behavioural pattern during calving time (8h before calving) in comparison with the previous phases, a significant increase in tail contraction frequency and raised tail position, and a decrease in eating behaviour and rumination time were observed. In fact, the first principal component (PC) was primarily explained by these variables, and calving cows best contributed to this PC. According to the results of the present study, the use of the MD can be a useful tool in detecting the moment of calving

Evaluation of antimicrobial and non-steroidal anti-inflammatory treatments for BRD on health and welfare in fattening bulls: a cross-sectional study

AbstractOur study aimed to evaluate the effect of different treatments for BRD on health and welfare in fattening bulls. A total of 264 bulls were enrolled. Welfare was assessed on day 2 (T0) and day 15 (T1) after arrival. A decrease in the welfare level was observed from T0 to T1. All bulls were inspected clinically at T0 and T1 revealing an increase of skin lesions and lameness in T1. In both periods, a high incidence of respiratory disease was observed. A prevalence of 79.55% and 95.45% of Mycoplasma bovis using RT-PCR and culture at T0 and T1 respectively was observed. Blood samples were collected for haematology at T0 and T1. At T0, 36 animals were individually treated for BRD with an antimicrobial (IT), 54 received a metaphylactic treatment with tulathromycin (M), 150 received a metaphylactic treatment with tulathromycin plus a second antimicrobial (M + IT) whereas 24 were considered healthy and therefore not treated (NT). Additionally, 128 were treated with a non-steroid anti-inflammatory (NSAID). Neutrophils of M + IT were significantly higher than groups NT and M and the lymphocytes of M + IT were significantly lower than that of IT. White blood cells, neutrophils and N/L ratio of animals treated with an NSAID was significantly higher than that not treated. Lung inspection of 172 bulls at the abattoir indicated that 92.43% presented at least one lung lesion. A statistically significant effect of the NSAID treatment on the lung lesions was observed. Our findings indicate that BRD was a major welfare and health concern and evidence the difficulties of antimicrobial treatment of M. bovis

PLATO: the ESA mission for exo-planets discovery

PLATO (PLAnetary Transits and Oscillation of stars) is the ESA Medium size dedicated to exo-planets discovery, adopted in the framework of the Cosmic Vision program. The PLATO launch is planned in 2026 and the mission will last at least 4 years in the Lagrangian point L2. The primary scientific goal of PLATO is to discover and characterize a large amount of exo-planets hosted by bright nearby stars, constraining with unprecedented precision their radii by mean of transits technique and the age of the stars through by asteroseismology. By coupling the radius information with the mass knowledge, provided by a dedicated ground-based spectroscopy radial velocity measurements campaign, it would be possible to determine the planet density. Ultimately, PLATO will deliver the largest samples ever of well characterized exo-planets, discriminating among their ‘zoology’. The large amount of required b right stars can be achieved by a relatively small aperture telescope (about 1 meter class) with a wide Field of View (about 1000 square degrees). The PLATO strategy is to split the collecting area into 24 identical 120 mm aperture diameter fully refractive cameras with partially overlapped Field of View delivering an overall instantaneous sky covered area of about 2232square degrees. The opto-mechanical sub-system of each camera, namely Telescope Optical Unit, is basically composed by a 6 lenses fully refractive optical system, presenting one aspheric surface on the front lens, and by a mechanical structure made in AlBeMet

Metis: the Solar Orbiter visible light and ultraviolet coronal imager

Aims. Metis is the first solar coronagraph designed for a space mission and is capable of performing simultaneous imaging of the off-limb solar corona in both visible and UV light. The observations obtained with Metis aboard the Solar Orbiter ESA-NASA observatory will enable us to diagnose, with unprecedented temporal coverage and spatial resolution, the structures and dynamics of the full corona in a square field of view (FoV) of ±2.9° in width, with an inner circular FoV at 1.6°, thus spanning the solar atmosphere from 1.7 R⊙ to about 9 R⊙, owing to the eccentricity of the spacecraft orbit. Due to the uniqueness of the Solar Orbiter mission profile, Metis will be able to observe the solar corona from a close (0.28 AU, at the closest perihelion) vantage point, achieving increasing out-of-ecliptic views with the increase of the orbit inclination over time. Moreover, observations near perihelion, during the phase of lower rotational velocity of the solar surface relative to the spacecraft, allow longer-term studies of the off-limb coronal features, thus finally disentangling their intrinsic evolution from effects due to solar rotation. Methods. Thanks to a novel occultation design and a combination of a UV interference coating of the mirrors and a spectral bandpass filter, Metis images the solar corona simultaneously in the visible light band, between 580 and 640 nm, and in the UV H