Antibacterial activity of oregano and sage plant extracts against decarboxylase-positive enterococci isolated from rabbit meat

The effect of plant extracts (sage, oregano) against decarboxylase-positive enterococci from rabbit back limb meat  was reported in this study. Oregano plant extract inhibited the growth of all 34 tested enterococci (the inhibitory zones: 12 to 45 mm). The growth of the majority of strains  (n=23) was inhibited by oregano plant extract (the high size inhibitory zones (higher than 25 mm). The growth of 11 strains  was inhibited by oregano extract reaching medium size inhibitory zones (10 to 25mm). The most sensitive strain to oregano extract was E. faecium M7bA (45 mm). Sage extract was less active against tested enterococci (n=16)  reaching lower inhibitory zones (up to 10 mm)

Enterococci Isolated from Japanese Quails Exposed to Microgravity Conditions and Stability of their Properties

Enterococci isolated from the crop and caecum of Japanese quails exposed to 7 day conditions of microgravity were re-vitalized after their dry-freezing long storage. Originally, the strains were isolated from Japanese quails after their landing from flight onboard the orbital station Mir during the experiment in August 1990. Because taxonomy as well as the studies concerning the bacteriocins, especially those produced by enterococci, have been continually developed for years, the aim of this study was to confirm species identification, stability of the properties of enterococci as well as to test new properties after their long storage. Genotyping allotted the strains to the species E. faecium. Lactic acid production was detected in similar amounts in the strains before and after their long-storage in dry-frozen form. The strains were vancomycinsensitive and kanamycin-resistant before as well as after their long-time storage. Variability in sensitivity to different antibiotics was found among the strains tested even before and after longtime storage. Each of the strains possessed at least one structural enterocin gene. The structural genes for enterocin A, P, B, L50B were detected in E. faecium EP7. E. faecium EP2, EEP4 have the genes for ent A, B, L50B. The gene for ent P was detected only in the strain EP7. The most often detected was ent A gene followed by ent genes B, L50B. All strains inhibited growth of at least 4 out of 15 indicators. The stability of the enterococcal properties determined before as well as after their dry-freezing was not influenced during their long-term storage; moreover, new properties were determined

Decarboxylase-positive Enterococcus faecium strains isolated from rabbit meat and their sensitivity to enterocins

Background: The objective of the study was to determine sensitivity of Enterococcus faecium strains from rabbit meat to enterocins. Results: Twenty-five decarboxylase-positive strains (from rabbit meat) allotted to the species E. faecium by genotypization and by MALDI TOF MS spectrometry identification (evaluation score value range 2.104–2.359; in the range for highly probable species identification-score value 2.300–3.000 and secure probable species identification/probable species identification-2.000–2.299) were studied. Seventeen strains were gelatinase positive. Although they did not produce histamine (HIS), spermidine, and spermine, they produce at least one among seven tested biogenic amines (BAs) in small amounts (2–10 mg/L) or up to very high amounts (>1000 mg/L). Putrescine was produced by two strains. These decarboxylase-positive strains were sensitive to enterocins (Ents). All strains were sensitive to Ent 2019 and Ent 55 (inhibitory activity from 200 to 819 200 AU/mL). Twenty-two strains were inhibited by Ent A(P) and Ent 4231; 20 strains were sensitive to Ent M. Conclusion: Our results have spread the basic knowledge related to inhibitory spectrum of enterocins showing sensitivity of decarboxylase-positive strains to enterocins. Protective possibilities of enterocins in meat processing were also indicated. © 2016 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.Slovak Scientific Agency VEGA [2/0004/14]; Slovak Research and Development Agency [SK-HU-0006-08

Microbial status and quality of rabbit meat after rabbits feed supplementation with phyto-additives

Nowdays, there is an increasing  interest in public health issue due to consumption of healthy and nutritive food, e.i. rich in proteins, low in cholesterol and lipid contents. The aim of this study was to examine if oregano, sage and Eleutherococcus senticosus extracts, commercial Xtract as well can influence microbial status of rabbit meat after their supplementation of rabbit feed. Reduction of enterococci, coagulase-negative staphylococci and Staphylococcus aureus after feed supplementation by the sage and oregano was more detectable at the end of the experiment (at day 42) than after their application (at day 21). In animals with Xtract addition, antibacterial effect has been noted already at day 21. Our in vivo results confirmed in vitro antibacterial effect of the tested extracts and showed that maintaining of rabbit meat quality by plant extracts is very promising

Factors affected decarboxylation activity of Enterococcus faecium isolated from rabbit

  Biogenic amines (BA) are basic nitrogenous compounds formed mainly by decarboxylation of amino acids. There are generated in course of microbial, vegetable and animal metabolisms. The aim of the study was to monitor factors affected production of biogenic amines by Enterococcus faecium, which is found in rabbit meat. Biogenic amines were analyzed by means of UPLC (ultrahigh performance liquid chromatography) equipped with a UV/VIS DAD detector. Decarboxylation activity of E. faecium was mainly influenced by the cultivation temperature and the amount of NaCl in this study. E. faecium produced most of the monitored biogenic amines levels: tyramine ˂2500 mg.l-1; putrescine ˂30 mg.l-1; spermidine ˂10 mg.l-1 and cadaverine ˂5 mg.l-1

Bacteriocin-producing strain lactiplantibacillus plantarum LP17L/1 isolated from traditional stored Ewe’s milk cheese and its beneficial potential

Research Areas: Food Science & TechnologyStored ewe’s milk lump cheese is a local product that can be a source of autochthonous beneficial microbiota, especially lactic acid bacteria. The aim of this study was to show the antimicrobial potential of Lactiplantibacillus plantarum LP17L/1 isolated from stored ewe’s milk lump cheese. Lpb. plantarum LP17L/1 is a non-hemolytic, non-biofilm-forming strain, susceptible to antibiotics. It contains genes for 10 bacteriocins—plantaricins and exerted active bacteriocin with in vitro antistaphylococcal and anti-listerial effect. It does not produce damaging enzymes, but it produces β-galactosidase. It also sufficiently survives in Balb/c mice without side effects which indicate its safety. Moreover, a reduction in coliforms in mice jejunum was noted. LP17L/1 is supposed to be a promising additive for Slovak local dairy products.info:eu-repo/semantics/publishedVersio

Beneficial effects of Enterococcus faecium EF9a administration in rabbit diet

[EN] Forty-eight rabbits aged five weeks (Hycole breed, both sexes) were divided into experimental (EG) and control (CG) groups, 24 animals in each, and fed a commercial diet with access to water ad libitum. Rabbits in EG had Enterococcus faecium EF9a probiotic strain added to their drinking water (1.0×109 colony forming units/mL 500 μL/d/animal) for 28 d (between 35 and 63 d). The experiment lasted for 42 d. The animals remained in good health condition throughout the experiment, and no morbidity and mortality was noted. There was a higher live weight at 63 d of age (+34 g; P<0.0001), final live weight at 77 d of age (+158 g; P=0.0483), and average daily weight gain between 63 and 77 d of age in the EG group rabbits than in CG group rabbits (+8 g/d; P<0.0001). No significant changes in caecal lactic acid and total volatile fatty acid concentrations, jejunal morphological parameters and phagocytic activity were noted during the treatment. The tested serum parameters were within the range of the reference values. EF9a strain sufficiently established itself in the rabbit’s gastrointestinal tract. At 63 d of age, a significant decrease in coliforms (P<0.05), coagulase-positive staphylococci (P<0.01), pseudomonads (P<0.01) and coagulasenegative staphylococci (CoNS, P<0.001) was noted in the faeces of the EG group rabbits compared to the CG rabbits. Antimicrobial effects of EF9a strain in the caecum against coliforms (P<0.001), CoNS (P=0.0002) and pseudomonads (P=0.0603) and in the appendix (coliforms, P<0.05) were detected.Slovak – Hungarian project APVV:SK-HU-0006-08 and the national VEGA project 2/0006/17 This work was financially supported by the bilateral Slovak – Hungarian project APVV:SK-HU-0006-08 and the national VEGA project 2/0006/17. Part of the preliminary results was presented in the Proceedings from the Conference in Kaposvár, Hungary, 30.05.2012, pp. 89-92. We are grateful to Mrs. M. Bodnárová and Mr. P. Jerga for their skilful technical assistance. We are also grateful to Dr. V. Párkányi and Dr. R. Jurčík, from the National Agricultural and Food Centre in Nitra for blood sampling and Mr. J. Pecho for slaughtering. All care and experimental procedures involving animals followed the guidelines stated in the Guide for the Care and Use of Laboratory Animals and the trials were accepted by the Ethic Commission of the Institute of Animal Physiology in Košice and by the Slovak Veterinary and Food Administration. We would like to thank to Mr. A. Billingham for English language correction.Pogány Simonová, M.; Lauková, A.; Chrastinová, Ľ.; Plachá, I.; Szabóová, R.; Kandričáková, A.; Žitňan, R.... (2020). Beneficial effects of Enterococcus faecium EF9a administration in rabbit diet. World Rabbit Science. 28(4):169-179. https://doi.org/10.4995/wrs.2020.11189OJS16917928

Enterococci from pannon white rabbits: detection, identification, biofilm and screening for virulence factors

[EN] Properties of enterococci isolated from the Hungarian breed Pannon White were studied to spread knowledge regarding the properties of microbiota in rabbits from the basic research standpoint and to select a beneficial candidate for application in husbandry. Faeces from 113 Pannon White rabbits (mostly maternal line and some paternal line) were collected. They were sampled from rabbits varying in age and sex (82 kits, 6 does, 6 bucks, 19 adult rabbits), which were aged 2 wk (14 suckling rabbits), 6 and 8 wk (68 weaning and post-weaning rabbits), adult rabbits (31, one year). Faecal mixtures were sampled into sterile packs with faeces from 5-6 animals in each. The total count of enterococci from these Pannon White rabbits reached, on av. 5.28±0.29 colony forming units/g (log10). Among the 19 enterococci, 14 E. faecalis and 5 E. faecium were detected using 3 identification methods. The enterococci were mostly resistant to antibiotics. They were non-haemolytic, Dnase and urease negative. They did not form biofilm. They were free of the hylEfm gene and IS16 genes; the most frequently detected genes were gelE, efaAfm, efaAfs. Based on these results, E. faecium EF9a was selected for further analysis.The results achieved were financially supported by the bilateral Slovak-Hungarian project APVV:SK-HU-0006-08 and partially by the projects Probiotech ITMS 26220220204 and Vega 2/0006/2017.Lauková, A.; Strompfová, V.; Szabóová, R.; Bónai, A.; Matics, Z.; Kovács, M.; Pogány Simonová, M. (2019). Enterococci from pannon white rabbits: detection, identification, biofilm and screening for virulence factors. World Rabbit Science. 27(1):31-39. https://doi.org/10.4995/wrs.2019.10875SWORD3139271Bagóne Vántus V., Dalle Zotte A., Cullere M., Bónai A., Dal Bosco A., Szendro Zs., Tornyos G., Pósa R., Bóta B., Kovács M., Zsolnai A. 2018. 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