Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect

The Family Health Support Core (NASF) And Health Practices: Are There Many Challenges To Be Overcome?

Objective: Describe and characterize NASF health practices. Method: It is a review of current literature carried out by consulting the database Lilacs and Virtual Library of SciELO in the period from December 2016 to January 2017. Conclusion: It is concluded that, although the NASF is recognized as a support to the Family Health Strategy (FHS), still does not act in an articulated way, being fundamental that changes take place in the organization of the services and in the conduct of the health professionals who contemplate it. Descriptors: Primary Health Care. Family Health; Public Health Policies. Nursing

Access Of Women In Jail To Cytological Exam: A Quantitative Analysis

Goal: to know the access of women prisoners to cytological examination. Method: exploratory-descriptive quantitative study, carried out at the Maria Júlia Maranhão Female Rehabilitation Center, in the city of João Pessoa/PB. The study population was of all closed regimens, and the minimum sample was 168 (one hundred and sixty-eight). Data collection was formalized through approval by the Research Ethics Committee of the Faculdade de Enfermagem Nova Esperança (FACENE), CAAE: 18363713.8.0000.5179, besides the official referral of the Coordination of the Course to said prison. The present study respected the ethical aspects recommended by Resolution CNS 466/12, in art. III, of the ethical aspects, which deals with the involvement with human beings in research, as well as Resolution COFEN 311/2007, which deals with the code of ethics of nursing professionals.   Results and Discussion: data from the survey show that 33% of study participants were between the ages of 18 and 25; 58% reported being single; 48% have Incomplete Elementary Education; 39% with the profession of domestic; 48% have a family income of one minimum wage; 58% have one to two years imprisonment; 74% inflicted the drug trafficking article; 67% mentioned working in general prison services.   Conclusion: it is concluded that the participants of the research report having knowledge about the exam, but their answers do not correspond to the true meaning of the exam, showing the lack of knowledge, information, assistance and abandonment in which they live.   Descriptors: Women. Papanicolaou Test. Prisoners. Nursing

Occurrence Of Osteomuscular Symptoms In Teachers Of A Higher Education Institution

The objective of this study was to investigate the occurrence of musculoskeletal symptoms in teachers of a Higher Education Institution (HEI) located in João Pessoa – PB. This is a descriptive research with a quantitative approach, held at the Faculties of Nursing and Medicine (FACENE/FAMENE), in the city of João Pessoa-PB. The instrument for data collection consisted of a form with objective questions and divided into two parts, the first with data characterizing the sample and the second with the Nordic Musculoskeletal Symptoms Questionnaire. The data were analyzed quantitatively in percentage numbers and distributed in graphs. The results indicated that 30 teachers accepted to participate in the study with female predominance (67%). The regions most affected in the last 12 months were: dorsal (60%), lumbar (53%), neck, shoulders, wrists and hands (47%). Of the teachers interviewed, 20% reported having had difficulties in routine activities due to discomfort in the cervical region and 27% reported having sought medical or physiotherapeutic care due to neck pain, while 20% due to pain in the shoulders, dorsal, wrist/hands and hip/thighs. As for the occurrence of symptoms in the last week, the regions with the highest frequency of pain were shoulders (40%), neck and dorsal (27%). The significant percentage in the regions cited certifies that they are the regions most burdened in the professors and, therefore, deserve special attention in possible projects of occupational preventive action. This research evidences the necessity of new approaches and deepening on the subject, including the formulation of intervention strategies in the process of sickness of teachers. Descriptors: Work-Related Musculoskeletal Disorders. University Teachers. Worker's Health

Difficulties Found By Persons Living With Diabetes Mellitus: A Quantitative Analysis

Goal: to know the main difficulties faced by DM patients, characterizing the socioeconomic situation of the interviewees, identifying the type of care received by DM patients. Method: It was a research of the descriptive exploratory type with quantitative approach that was carried out in the Basic Health Care of the city of Camutanga/PE. The population was formed by one hundred (100) diabetic patients enrolled at the FHS of said municipality, the sample will be formed by one hundred (100) of diabetics from UBS Mini Posto. The instrument for data collection was a form. The data collection took place in September and October 2016 after approval by the Research Ethics Committee of the Faculdade de Enfermagem Nova Esperança (FACENE). CAAE: 59111616.1.0000.517 Results and Discussion: the data obtained in the research show that 65% of the study participants are between the age group over 65 years; 67% have primary education; 53% are retired; 62% reported receiving a minimum wage. About the data related to the issue 31% report that they have diabetes between 05 to 10 years; 79% prevail with type 2; 100% make use of medication; 43% use glibenclamide and metformin. Conclusion: in view of the foregoing, it is concluded that this municipality presents several positive points in caring for the patients, but there are still gaps to provide adequate care to the carriers.              Descriptors: Diabetes Mellitus. Primary Health Care. Nursing

Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

<div><p>Abstract Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.</p></div

Características de pelame e respostas fisiológicas de ovelhas nativas da região Semiárida do Brasil

This study aimed to evaluate the effects of heat stress and characterize the coat characteristics of red and white varieties of Morada Nova sheep raised in the semiarid region of Brazil during the rainy and dry seasons. The following variables were investigated: coat thickness (CT), inclination angle of the coat (?I), hair coat density (HDE), hair length (HL) and hair diameter (HD), in conjunction with the physiological responses of rectal temperature (RT) and respiratory rate (RR). The data were tested with an analysis of variance, a Tukey test at a 5% significance level and a correlation analysis. The season of the year (dry and rainy) and the variety (red and white) were used as fixed effects in the analysis of variance. HL, HDE and HD were significantly greater in the dry season than in the rainy season (P 0.05). HL, HDE and HD were lower and ?I was greater (P 0.05) for the two varieties. It was concluded that the coat characteristics of the Morada Nova ewes favor the adaptation of the sheep to a semiarid environment, including the improved protection of the skin against ultraviolet radiation. In general, the white variety presented characteristics reflecting a higher degree of adaptation to the conditions of the Brazilian semiarid region.O objetivo desse trabalho foi avaliar as características de pelame de ovelhas da variedades vermelha e outra branca da raça Morada Nova, criadas no ambiente semiárido brasileiro, durante as épocas seca e chuvosa. Foram avaliada espessura de pelame (CT), ângulo de inclinação do pelo (I), número de pelos (HDE), comprimento do pelo (HL) e diâmetro do pelo (HD), em conjunto com as respostas fisiológicas temperature retal (RT) e frequência respiratória (RR). Foram realizados análise de variância e teste de Tukey a 5% de probabilidade. A estação do ano (seca e chuvosa) e variedade (vermelha e branca) foram usadas como fatores fixos. HL, HDE e HD foram significativamente maiores na estação seca (P < 0,05), mas CT e ?I não diferiram. HL, HDE e HD foram menor e ?I foi maior na variedade vermelha, CT não apresentou diferença em relação a variedade. Conclui-se que as características de pelame das ovelhas Morada Nova favorecem a adaptação a ambiente semiárido, incluindo proteção da pele contra o excesso de raios ultravioleta, com a variedade branca apresentando-se mais favorável à adaptação nas condições da região semiárida brasileira