12 research outputs found
Bioortogonális jelzĂ©sre alkalmas azid tartalmĂş fluorogĂ©n jelzĹ‘vegyĂĽletek tervezĂ©se, előállĂtása Ă©s tesztelĂ©se
Bioorthogonal fluorescent labels: a review on combined forces
This review ventures to summarize the latest developments in
bioorthogonal fluorescent imaging labels with a special focus
on bioimaging applications. We briefly summarize the most
preferred means of bioorthogonal tagging schemes for the
labeling of specific biomolecular structures. The review is
structured by the type of the fluorescent labels that can
address the problems that most commonly compromise
fluorescent imaging techniques, i.e. the autofluorescence of
biomolecules, the background fluorescence of unreacted
reagents, and photobleaching. Thus, we present (i) far-
red/near-infra-red emitting dyes, (ii) fluorogenic scaffolds,
and (iii) nanoparticle-based signaling platforms
Synthesis of 7-azido-3-formylcoumarin – a key precursor in bioorthogonally applicable fluorogenic dye synthesis
Tyrosine specific sequential labeling of proteins
We report (a) on the synthesis of a long-wavelength fluorescent coumarin containing an allyloxy acetate moiety, (b) the synthesis of two linkers containing an allyloxy acetate and an alkyne or azide function, respectively, and (c) the selective modification human serum albumin by a sequential method involving Pd(II) catalyzed modification of the phenolic side chain of tyrosine residues with an alkyne bearing linker and a subsequent azide-alkyne click reaction with an azide functionalized long-wavelength emitting coumarin dye. The method is likely to be applicable to various kinds of azido-modified fluorophores, and the Pd(II)-catalyzed modification of the tyrosines may also be used to introduce other kinds of tags. With these reagents, tyrosine specific modulation of proteins and peptides becomes possible either directly or in a sequential manner
New Generation of Bioorthogonally Applicable Fluorogenic Dyes with Visible Excitations and Large Stokes Shifts
Magángyűjtemények a királyi Magyarországon és az Erdélyi fejedelemségben, 1533–1721
Clickable fluorophores for biological labeling—with or without copper
The synthesis of a set of new clickable fluorophores that virtually cover the whole visible spectrum reaching the near infra-red regime is presented herein. Besides dyes that are capable of participating in classical copper catalyzed 1,3-dipolar cycloaddition reactions with the counterparting function we have also prepared dyes containing a cyclooctyne moiety, an alkyne derivative that enables copper free clicking to azides. The suitability of these dyes for fluorescent labeling of biomolecules is presented by examples on model frameworks representing major biopolymer building blocks. The versatility of these dyes is presented in cell labeling experiments as well as by labeling the azide modified surface glycans of CHO-cells either by copper catalyzed or copper-free click reaction. These dyes are expected to have a large variety of applications in (bio)orthogonal labeling schemes both in vivo and in vitro