SPECIES IDENTIFICATION OF ASTERACEAE FAMILY AT UNIVERSITAS INDONESIA, DEPOK

Penelitian identifikasi Asteraceae di Kampus Universitas Indonesia Depok di lakukan pada bulan September hingga Oktober 2012. Tujuan penelitian adalah untuk mengetahui jenis-jenis Asteraceae yang terdapat di Kampus Universitas Indonesia, Depok.  Tanaman didentifikasi berdasarkan karakter morfologi menggunakan kunci determinasi buku Flora of Java dan buku morfologi tumbuhan.  Terdapat 21 jenis dari 20 marga Asteraceae tersebar di Kampus Universitas Indonesia Depok.  Asteraceae yang ditemukan di Kampus Universitas Indonesia tersebar di tempat - tempat yang terpapar cahaya matahari seperti lapangan, jalan raya, lahan bangunan, perbatasan hutan dan selokan.  Lokasi yang memiliki jumlah jenis Asteraceae terbanyak adalah Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia dengan jumlah total jenis 16.  Asteraceae yang umum dijumpai di Kampus Universitas Indonesia Depok yaitu Mikania micrantha, Cyanthilliumcinereum, Synedrella nodiflora, Ageratum conyzoides, Tridax procumbens, dan Emilia sonchifolia. Keywords: Identification, description, morphology, Asteraceae, University of Indonesi

Asteraceae Diversity and A New Record For Java at Citalahab Village, Gunung Halimun-Salak National Park

Asteraceae is the second largest plant family in the world. The family member has reached 227 species in Java. However, there is no current record of wild Asteraceae around local village within Gunung Halimun-Salak National Park. This study is to provide current Asteraceae species data and the threat for the conservation area. Explorative method has been conducted in 6 sites. The result shows that there are 20 species found with the tribes composition are 8 Heliantheae, 6 Eupatorieae, 3 Senecioneae, 1 Astereae, 1 Cichorieae, and 1 new record Vernonieae in Java. Key identification for species are provided and the new record has been described. Most species categorized as introduced with several other categorized as invasive alien species. In conclusion, numbers of Asteraceae family has been recorded with some potential ivansive threat in Gunung Halimun-Salak National Park. Regular population control and treatment are recommended in order to protect native species in the conservation area

Local adaptation of invasive plant, Synedrella nodiflora, in urban tropical lowland landscape Universitas Indonesia

Synedrella nodiflora is an invasive species originated from tropical America and now has spread throughout Indonesia. We analysed the ability of Synedrella nodiflora from the level of HSP70 gene expression at different heat stress in urban tropical lowland landscape Universitas Indonesia.  We used the qPCR to quantify the level of HSP70 gene expression and analysed using Pfaffl model. We found the level of HSP70 gene expression got higher related to elevated temperature from 29oC to 39oC with a range of fold from 123.1 to 1676.9. This ability reflects the adaptive plasticity of Synedrella nodiflora in the course of the invasion process.

The influence of season on phenotypic plasticity symptoms in Hibiscus rosa-sinensis crested peach flower

The variation of Hibiscus rosa-sinensis flower in the form of a crested peach is thought to show phenotypic plasticity symptoms in nature. Phenotypic plasticity is a condition in which genotypes give rise to different phenotypes in different environments. The aim of this study was to determine the plasticity response of H. rosa-sinensis crested peach flowers to seasonal changes, both morphologically and anatomically. Flower samples were taken from the residential area of Bojong Gede, Bogor, in two different seasons: the dry season in 2018 and the rainy season in 2021. Morphological observations were made by calculating the number and size of each flower section using measuring instruments and a Dino-Lite microscope. Anatomical observations were made by observing the internal structure of the ovaries using a 4× magnification light microscope. Measurement of environmental parameters, such as temperature, humidity, and light intensity, was also carried out in this study to determine the symptoms of phenotypic plasticity. The phenotypic plasticity responses of H. rosa-sinensis crested peach flower are clearly observed in the number and composition of the stamen, staminodium petaloid, intermediate stamen-petal, and external-internal structure of the ovary. H. rosa-sinensis, in the form of crested flowers, showed a different phenotypic plasticity response in different seasons. The light intensity and temperature factors play an important role in phenotypic plasticity. Research with various observation times is still needed to determine the range of phenotypic plasticity responses of the H. rosa-sinensis flower form of crested peach in nature

INDEKS MITOSIS PUCUK DAUN Hibiscus rosa-sinensis L. VARIASI SINGLE PINK PADA BEBERAPA VARIASI WAKTU

AbstrakHibiscus rosa-sinensis L. atau kembang sepatu merupakan tanaman hias yang memiliki banyak manfaat dan merepresentasikan sifat poliploidi. Tujuan penelitian ini yaitu untuk mengetahui Indeks Mitosis (IM) dan jumlah kromosom pucuk daun H. rosa-sinensis pada beberapa variasi waktu. Indeks Mitosis dan waktu pengambilan pucuk sangat diperlukan untuk studi kromosom karena pada tahap tersebut karakter-karakter kromosom dapat diamati dengan jelas dan mudah dihitung. Waktu pengambilan pucuk yang dilakukan yaitu pada 08:00, 10:00, 12:00, 14:00, 16:00 WIB. Pembuatan sediaan kromosom dilakukan menggunakan metode squash menggunakan pewarna Aceto-orcein. Tahapan perlakuan meliputi perendaman pucuk daun di dalam air dingin selama 3 jam, fiksasi dalam larutan Carnoy selama ±24 jam, dan hidrolisis dalam larutan HCl 5N selama 30 menit. Hasil penelitian menunjukkan nilai IM tertinggi meristem pucuk daun H. rosa-sinensis variasi single pink besar muncul pada pukul 10:00 sebesar 94%. Berdasarkan hasil tersebut dapat disimpulkan bahwa waktu sampling yang optimal untuk analisis kromosom H. rosa-sinensis L. variasi single pink besar adalah pukul 10:00 dengan jumlah kromosom 2n= ca. 69-111.  Hasil penelitian ini dapat dijadikan dasar untuk studi kromosom selanjutnya serta acuan untuk sampling variasi lainnya.Abstract Hibiscus rosa-sinensis L. is an ornamental plant that has many benefits and represents the character of polyploidy. The purpose of this study is to find out the Mitotic Index of leaf shoots Hibiscus rosa-sinensis on several shoots sampling times. The Mitotic Index and the timing of shoots sampling time are very necessary for chromosome studies because at this stage chromosomes characters can be clearly observed and easily calculated. Period time of collection the leaf shoots is from 08:00 AM to 16:00 PM, with two hours interval each at 08:00, 10:00, 12:00, 14:00, 16:00 . The chromosome preparation was carried out by using the squash method and aceto-orcein staining. The treatment steps included soaking the leaf shoots in cold water for 3 hours, fixation in Carnoy solution for ± 24 hours, and hydrolysis in 5N HCl solution for 30 minutes. The results showed that chromosomes were clearly visualized during the phase with the highest Mitotic Index.  In addition, the percentage of Mitotic Index was found to be in line with the percentage of cells in late prophase. Among several sampling time variations, the highest Mitotic Index of Hibiscus rosa-sinensis leaf shoots appeared at 10:00 at 94% with the chromosome numbers of 2n= ca. 69-111. According to the data obtained, it is concluded that 10 AM is the most optimum sampling time that can be used as the basic information for further chromosome studies

VARIATION IN THE COMPOSITION AND STRUCTURE OF NATURAL LOWLAND FORESTS AT BODOGOL, GUNUNG GEDE PANGRANGO NATIONAL PARK, WEST JAVA, INDONESIA

An analysis of the composition and structure of lowland natural forests was carried out in Bodogol, Gunung Gede Pangrango National Park (GGPNP). The two study plots (P1CS and P2CS) were located on Cisuren and one plot (P3CP) on Cipadaranten hill. We recorded 107 species and 48 families with an average basal area of 19.73 m2/ha, and an average density of 348 trees/ha. The species richness was poorer than those of the typical lowland rainforests of Kalimantan and Sumatra but comparable to those of the montane forests of Java. The IUCN-Red Listed species were Castanopsis argentea and Castanopsis tungurrut (critical) and Saurauia bracteosa (vulnerable). Based on the two dominant species, the forests can be designated as the Maesopsis eminii-Syzygium acuminatissimum association and Syzygium acuminatissimum-Lithocarpus korthalsii association. Maesopsis eminii was dominant in P1CS (IV= 56.46%) and P3CP (IV=55.94%), while Syzygium acuminatissimum in P2CS (IV= 43.67%). Maesopsis eminii was a strongly aggressive and invasive species, that endangered the purity of the natural forest GGPNP, therefore, it must be eradicated. Vertically, P2CS and P3CP consisted of four strata, while P1CS had three strata. This one-hectare study can be considered as a minimal area to reflect the floristic representation of lowland forest and submontane forest

FAKTOR YANG BERHUBUNGAN DENGAN KUNJUNGAN NIFAS DI WILAYAH KERJA PUSKESMAS SAMATA 2019-2020: Related Factors of Postpartum Visit in the Working Area of Puskesmas Samata 2019-2020

Salah satu keberhasilan masa nifas adalah dengan melakukan pencegahan kematian ibu pada masa nifas. WHO memperkirakan sekitar 10,7 juta perempuan di dunia yang meninggal karena melahirkan dan 25-50% penyebabnya ialah masalah kesehatan, persalinan dan nifas. Penelitian ini bertujuan untuk mengetahui hubungan umur, pekerjaan, pendidikan, jarak kehamilan, dan paritas terhadap kunjungan ibu nifas di wilayah kerja Puskesmas Samata Gowa Tahun 2019-2020. Jenis penelitian ini adalah penelitian analitik dengan desain cross- sectional study. Populasi penelitian ini adalah semua ibu nifas yang tercatat berkunjung di buku register Puskesmas Samata Gowa dalam rentang waktu dimulai pada bulan Januari 2019 sampai dengan Oktober tahun 2020 yaitu sebesar 489 orang. Teknik pengambilan sampel yang digunakan yaitu simple random sampling dengan jumlah sampel sebanyak 141 ibu nifas. Analisis data menggunakan analisis univariat dan bivariat. Menunjukkan bahwa sebanyak 41.8% responden yang tidak lengkap melakukan kunjungan nifas dan 58.2% yang lengkap melakukan kunjungan nifas. Tidak terdapat hubungan antara umur (p=0,602), pekerjaan (p=1,000), pendidikan (p=0,955), jarak kehamilan (p=0,353). Namun, penelitian ini menunjukkan terdapat hubungan antara partus (p=0,038) dengan kunjungan ibu nifas di Puskesmas Samata Gowa. Penelitian ini dapat diketahui bahwa ada hubungan antara paritas dan kunjungan ibu nifas. Sedangkan untuk umur, pekerjaan, pendidikan, dan jarak kehamilan tidak adanya hubungan dengan kunjungan ibu nifas

PENGUJIAN KEMAMPUAN ANTAGONISTIK KHAMIR Rhodotorula spp. ASAL KEBUN RAYA CIBODAS TERHADAP KAPANG DARI TANAMAN TOMAT TERINFEKSI DENGAN CO-CULTURE

Aspergilus ochraceu and A. terreus had been known as mould that causes significant loss to quality of tomatoes. One of potential methods to control the development of this mould by using the antagonistic activity of Rhodotorula sp. Six strains of Rhodotorula sp., UICC Y-318, UICC Y-325, UICC Y- 332, UICC Y-381, UICC Y-384, and UICC Y-386, which isolated from Cibodas Botanical Garden, West Java were observed for its antagonistic activity against mould A. ochraceu D1.2.2.2.SSM3 and A. terreus D2.2.MC. Co-culture method was used as 1.58 to 5.59 x 108 CFU/ml Rhodotorula sp. growth together with 7 x 107 CFU/ml A. ochraceus D1.2.2.2.SSM3 and 7.5 x 107 CFU/ml A. terreus D2.2.MC at PDB. Result showed that the highest percentage reduction of conidial heads and hyphal width was show by Rhodotorula sp. UICC Y-318 against A.ochraceus (9.45% and 12.43%; 7.10% and 7.51%, 2 and 3 day after incubation, respectively)

TRAIT-BASED STUDIES ON THE ABUNDANCE AND CANOPY SHADE PREFERENCES OF ASTERACEAE SPECIES IN CIBODAS BOTANICAL GARDEN

ABSTRACT Knowledge of community structures and capacity to differentiate invasive from non-invasive species are essential for invasive species management. Specific Leaf Area (SLA) is a potential proxy to differentiate invasive from non-invasive species. This study aims to identify the relationship between Important Value Index (IVI) with SLA of Asteraceae species, and predict Asteraceae habitat preference based on shade characteristics at the Cibodas Botanical Garden. There is a positive correlation between SLA and IVI value of Asteraceae species in shaded habitat. The Asteraceae species that prefer shaded habitat tend to have larger SLA relative to species in open area (odds ratio = 2.754). This study showed that SLA is a potential proxy to indicate plant abundance and an informative proxy to predict habitat preferences in CBG ecosystem. These traits information are crucial for invasive exotic plant species management in CBG particularly and tropical botanic gardens in general.   Keywords: invasive species, exotic species, SLA, logistic regression, trait, Cibodas, Asteracea