Application of gelatin coating incorporated with cinnamon essential oil on shelf life of rainbow trout (Oncorhynchus mykiss) fillet in refrigerated storage

In this study, the effect of gelatin coating enriched with cinnamon oil for fresh fillet of rainbow trout on microbial were studied, chemical and sensory characteristics during storage at refrigerator condition. Fish fillet were treated in gelatin solution (4 percent) containing cinnamon oil and were stored in refrigerator. Total viable count was acceptable (7 log CFU/g) for coated fillets with gelatin coating containing cinnamon essential oil on day 15, but the control fillets also those that were covered with gelatin receipt to 7.88 and 7.44, respectively. In the tenth day psychrotrophic count values for the control and gelatin coated fillets significantly were increased more than other treatment. Total volatile bases nitrogen and Thiobarbituric acid values in gelatin with cinnamon coated fillet were less than other treatment. In general, results suggest successful inhibition microbial growth in refrigerated rainbow trout fillet is possible with gelatin incorporated cinnamon coating, as together they kept the sensory characteristics within acceptable limits throughout storage. Gelatin coating together with cinnamon oil provides a type of active coating that can be utilized as a safe preservative for fish under refrigerated storage

MicroRNAs and Periodontal Disease: Helpful Therapeutic Targets?

Periodontal disease is the most common oral disease. This disease can be considered as an inflammatory disease. The immune response to bacteria accumulated in the gum line plays a key role in the pathogenesis of periodontal disease. In addition to immune cells, periodontal ligament cells and gingival epithelial cells are also involved in the pathogenesis of this disease. miRNAs which are small RNA molecules with around 22 nucleotides have a considerable relationship with the immune system affecting a wide range of immunological events. These small molecules are also in relation with periodontium tissues especially periodontal ligament cells. Extensive studies have been performed in recent years on the role of miRNAs in the pathogenesis of periodontal disease. In this review paper, we have reviewed the results of these studies and discussed the role of miRNAs in the immunopathogenesis of periodontal disease comprehensively. miRNAs play an important role in the pathogenesis of periodontal disease and maybe helpful therapeutic targets for the treatment of periodontal disease

Evidence for a Resting State Network Abnormality in Adults Who Stutter

Neural network-based investigations of stuttering have begun to provide a possible integrative account for the large number of brain-based anomalies associated with stuttering. Here we used resting-state EEG to investigate functional brain networks in adults who stutter (AWS). Participants were 19 AWS and 52 age-, and gender-matched normally fluent speakers. EEGs were recorded and connectivity matrices were generated by LORETA in the theta (4–8 Hz), alpha (8–12 Hz), beta1 (12–20 Hz), and beta2 (20–30 Hz) bands. Small-world propensity (SWP), shortest path, and clustering coefficients were computed for weighted graphs. Minimum spanning tree analysis was also performed and measures were compared by non-parametric permutation test. The results show that small-world topology was evident in the functional networks of all participants. Three graph indices (diameter, clustering coefficient, and shortest path) exhibited significant differences between groups in the theta band and one [maximum betweenness centrality (BC)] measure was significantly different between groups in the beta2 band. AWS show higher BC than control in right temporal and inferior frontal areas and lower BC in the right primary motor cortex. Abnormal functional networks during rest state suggest an anomaly of DMN activity in AWS. Furthermore, functional segregation/integration deficits in the theta network are evident in AWS. These deficits reinforce the hypothesis that there is a neural basis for abnormal executive function in AWS. Increased beta2 BC in the right speech–motor related areas confirms previous evidence that right audio–speech areas are over-activated in AWS. Decreased beta2 BC in the right primary motor cortex is discussed in relation to abnormal neural mechanisms associated with time perception in AWS

Inclination Sensor With a Wide Angle Measurement Range Using Half-Wavelength Microstrip Resonator

In this article, a new inclinometer concept based on an angular half-wavelength microstrip resonator is presented. The inclinometer has a wide dynamic range of 84° (B142°) which can be extended to 180° through a simple modification. The inclinometer is composed of a microstrip transmission line resonator on printed circuit board and a second transmission line on an overlapping circuit board that rotates according to the inclination angle. As the inclination angle changes, the overall length of the resonator varies and leads to resonance frequency shift, which is transformed into inclination variation. The sensor measurements results determined the sensitivity of the sensor as 0.384 mm/° and the resolution as 0.035°. The proposed sensor is also quite simple, cost-effective, compact, and robust

Extraction, partial purification and characterization of alkaline protease from rainbow trout (Onchorhynchus Mykiss) viscera

In this study, crude alkaline proteases were recovered from rainbow trout (Oncorhynchus mykiss) viscera and partially purified by use of different saturation of ammonium sulfate. The enzyme exhibited highest yield, purity and activity when precipitated at a saturation of 40–60% compared to other ranges of saturation. Molecular weight for extracted protease was between 8-24 kDa. The protease had caseinolytic activity over a wide range of temperatures (30-55 °C) and pH (4-12). Soybean trypsin inhibitor and trypsin-chymotrypsin inhibitor strongly inhibited the enzyme activity but it was stable in the presence of surfactant, oxidizing reagents and organic solvents. The proteases had serine protease activity but no collagenase activity was detected. The current study showed that partially purified protease from the digestive tract of rainbow trout could be applicable in food and detergent industry because of its good activity over a wide temperature and pH range and its good thermal stability

Effects of atrovastatin on concentrations of 3-hydroxy-3-methylglutaryl-coenzyme A- reductase (HMG-CoA-R), proprotein convertase subtilisin/kexin type 9 (PCSK9) and sortilin in patients with type 2 diabetes mellitus and pre-diabetics

Introduction: Atorvastatin hinders cardiovascular disease by reducing cholesterol levels. Proprotein convertase subtilisin/kexin type 9 (PCSK9) enhances the secretion of insulin by binding to LDL receptor. Sortilin is committed in the transfer of intracellular proteins through the plasma membrane. Objectives: The purpose of this research was to determine the effect of atorvastatin consumption on alterations in the levels of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R), PCSK9 and sortilin in diabetic patients and pre-diabetics. Patients and Methods: This study was carried out on 80 individuals including normal subjects, diabetic patients and pre-diabetics. The participated individuals were divided as control group (i) (healthy individuals without diabetes mellitus), diabetic group receiving statin (ii), diabetic group not receiving statin (iii), pre-diabetic group receiving statin (iv) and pre-diabetic group not receiving statin (v). Levels of HMG-COA-R, PCSK9 and sortilin were determined by ELISA method. Results: In diabetics and pre-diabetics taking atorvastatin, the level of HMG-COA-R was not altered significantly compared to diabetics and pre-diabetics not taking atorvastatin, respectively (P> 0.05). The serum PCSK9 level in diabetics and pre-diabetics was significantly higher than the healthy individuals (P= 0.001). Additionally, the serum PCSK9 level in diabetics and pre-diabetics receiving atorvastatin was significantly higher than diabetics and pre-diabetics not receiving atorvastatin, respectively (P=0.001). The serum sortilin level in diabetics and pre-diabetics was significantly higher than the healthy individuals (P=0.001). In addition, the serum sortilin level in pre-diabetics receiving atorvastatin was significantly higher than pre-diabetics not receiving atorvastatin (P=0.001). Conclusion: Atorvastatin improved insulin secretion and sensitivity by increasing serum sortilin and PCSK9 levels. Thereby, it prevented the development of diabetes in diabetics and the progression of pre-diabetes to diabetes in pre-diabetics

Folklore medicinal plants used in liver disease: A review

The liver is the largest organ of the body and the main site of essential biochemical reactions in the human body. This is useful for detoxification of toxic substances and the production of biological molecules. Therefore, liver damage leads to severe consequences. Because herbs and herbal antioxidants are used to detoxify and treat liver disorders, this review was conducted to report the most important medicinal plants affecting liver disorders and diseases. Key terms liver cancer, medicinal plants, liver disorder and medicinal plants, liver disease and medicinal plants, effect of extract and essential oil of effective medicinal plants on liver tissues in rats, mice, and laboratory mice, and effect of extract and essential oil of medicinal plants on liver disease were used to retrieve relevant publications indexed in databases IranMedex, Irandoc, ISI, PubMed, Scopus, SID, Magiran, and Google Scholar. Based on the evidence found in this review, the medicinal plants Zingiber officinale, Cucurbita pepo, Citrus reticulate, Petroselinum crispum, Andrographis paniculata, Silybum marianum, Camellia sinensis, Nasturtium officinale, Physalis peruviana, Thonningia sanguinea, Nigella sativa, Cichorium intybus L., Terminalia catappa, Glycyrrhiza glabra, Curcuma zanthorrhiza, Hibiscus sabdariffa, Vaccinium vitis-idaea, Salvia miltiorrhiza, Kigelia africana, Alchornea cordifolia, Boerhavia diffusa, Schisandrae chinensis, Tinospora cordifolia, Brassica rapa subsp. rapa, Lygodium flexuosum, Carica papaya, Solanum fastigiatum, and Cheilanthes farinosa are some of the most important medicinal plants affecting liver disorders and diseases. © 2018 BRNSS Publication Hub. All rights reserved