To Evaluate the effect of Ashwatha on Mutrakrichhra w.s.r. to E.coli

In Ayurvedic classics E.Coli infection is not mentioned, but Krimis and Mutrakrichhra are mentioned. Most of the symptoms of Mutrakrichhra are similar with Escherichia coli infection. Escherichia coli is the major cause of urinary tract infections. The disease Mutrakrichhra has been described by almost all Acharyas. UTI is a major problem in society which causes Mutravaha Srotas Vikriti. Modern medical science physicians are facing problem in the treatment of E.coli. Infection, due to its relapse and synthetic antibiotic hazards. Due to this reason present study was planned to evaluate the efficacy of Ashawath which have been used by ancient Acharyas in Mutravaha Srotas Vikriti. In view of above fact the present trial has been undertaken to understand the effect of Ashawatha having antibacterial and diuretic properties. The present research work has been carried out with a view to provide a scientific basis for the claims made in various Ayurvedic texts regarding the effect of Shigru Ashwatha Ghana on Mutrakrichhra (UTI)

To evaluate the efficacy of Shigru in Mutrakrichhra w.s.r. to E.Coli

All the Acharyas have described the pathogenesis of Mutrakrichha and Vasti (bladder) Rogas. Acharya Charaka has given specific pathogenesis of Mutrakrichha, that Doshas being provoked by their respective aetiological factors, individually or altogether reaches Vasti and Mutramarga and begins to compress on all sides and thus results in Mutrakrichha. The vitiated Doshas enter into Vasti, which is the place of Khavaigunya. Dosa Dushya Sammurchhana takes place and Mutradusti occurs. Dushita Mutra causes irritation and spasm of urinary tract and leads to difficulty in micturition. Sushruta in general has explained the pathogenesis of Vasti Roga and Vayu as the main causative factor. Samyak Apana Vayu helps in proper excretion of urine from Vasti and when this Vayu becomes Pratiloma (opposite) produces disorders in Vasti

Characterization of color cross-talk of CCD detectors and its influence in multispectral quantitative phase imaging

Multi-spectral quantitative phase imaging (QPI) is an emerging imaging modality for wavelength dependent studies of several biological and industrial specimens. Simultaneous multi-spectral QPI is generally performed with color CCD cameras. However, color CCD cameras are suffered from the color crosstalk issue, which needed to be explored. Here, we present a new approach for accurately measuring the color crosstalk of 2D area detectors, without needing prior information about camera specifications. Color crosstalk of two different cameras commonly used in QPI, single chip CCD (1-CCD) and three chip CCD (3-CCD), is systematically studied and compared using compact interference microscopy. The influence of color crosstalk on the fringe width and the visibility of the monochromatic constituents corresponding to three color channels of white light interferogram are studied both through simulations and experiments. It is observed that presence of color crosstalk changes the fringe width and visibility over the imaging field of view. This leads to an unwanted non-uniform background error in the multi-spectral phase imaging of the specimens. It is demonstrated that the color crosstalk of the detector is the key limiting factor for phase measurement accuracy of simultaneous multi-spectral QPI systems.Comment: 16 pages, 8 figure

Ensuring On-Farm Production and Utilization of Fodder Planting Materials: A Case of Hybrid Napier in Rural Uttar Pradesh

Fodder and livestock rearing is sine-qui-non to each other. Feeding assumes highest share (60-70%) of total cost involved in livestock production. Making the green fodder available round the year to the cattle keepers is a great challenge. The country faces the current deficit of green and dried fodder to the extent of 40 percent. The current level of milk production in India to the extent of 128 mt which is expected to be 160 mt by 2050 demands 494 mt dried fodder, 825 mt green fodder and 54 mt of concentrate feed. Probably, it is beyond the capacity of any public or private sector organization to ensure the readily availability of seed and planting materials of fodder crops. The circumstances, thus, prompts to seek the alternative viable option for mitigation of on-farm fodder planting materials\u27 availability. This paper analyzes how empowering farmers for farmer-centric production and dissemination of planting materials for fodder crops can be evolved and validated

Reproductive endocrine effects of intranasal administration of norethisterone to adult female rhesus monkeys (Macaca mulatta)

Intranasal administration of norethisterone at a daily dose of 9 μg between Days 5 and 14 of the menstrual cycles blocked ovulation in 10 out of 17 adult female monkeys. Serum concentrations of hormones indicated that ovulation was blocked due to a suppression of the mid-cycle, oestradiol-induced LH surge. Ovarian follicular activity in the treated menstrual cycles was not affected by norethisterone but there was a marked delay in the onset of the mid-cycle oestradiol surge in most of the treated animals. The duration of the menstrual cycle length after the oestradiol peak was significantly reduced in all the treated monkeys, indicative of a shortened luteal phase

Evaluation of antioxidant potential of alcoholic stem bark extracts of Bauhinia variegata Linn.

The present study was carried out for the evaluation of in-vitro antioxidant potential of alcoholic stem bark (ALSB) extract of Bauhinia variegata Linn. Dried stem bark extract of B. variegata Linn. was screened to evaluate its free radical scavenging effect. Various methods like DPPH assay, reducing power assay, percentage scavenging activity of hydrogen peroxide and superoxide radical scavenging activity were used for screening in-vitro antioxidant potential. Antioxidant potentials were concentration dependent which were compared with standard antioxidants such as butylated hydroxyanisole (BHA) and ascorbic acid. The maximum scavenging effect of B. variegata Linn. alcoholic stem bark extract on  DPPH free radical, superoxide radical and hydrogen peroxide was 72.19 + 0.20, 81.60 + 0.22 and 76.06 + 0.16 respectively at a concentration of 2500 ?g/mL.  It was clearly indicated that the alcoholic extract of the stem bark has significant in vitro antioxidant activity. Currently available synthetic antioxidants like butylated hydroxyanisole (BHA), butylated hydroxyl toluene (BHT), Ascorbic acid and gallic acid appear to be associated with hepatotoxicity and many others negative health effects. Therefore, natural antioxidants may be preferred over the synthetic antioxidants

Circadian and circannual changes in the testicular function of adult rhesus monkeys

The endocrine and gametogenic status of the testes were studied in 9 healthy adult rhesus monkeys of proven fertility throughout a one-year period. Testosterone levels were estimated by radioimmunoassay in blood samples collected at 4 h intervals over a 24 h period once a month. Semen samples and testicular biopsies were also examined once a month. A well-defined circandian rhythm was evident in serum levels of testosterone. The rhythmicity was less pronounced in February and September. The 24 h mean levels of serum testosterone were high between the months of August to March and low in the months of May to July. All animals did not uniformly respond to electro-ejaculation in April and May. Semen volume and total number of spermatozoa were maximal between September and March and least from April to August. Testicular biopsies indicated that all stages of spermatogenesis were evident between September and March and the spermatogenic activity was less evident between April and August. The contents of Sertoli cells showed a seasonal cyclicity; they were laden with lipid droplets during April to August when spermatogenesis was quiescent and vacuolated during September to March when spermatogenesis was active. These studies indicate that the testing of contraceptive drugs needs to be restricted to months of September to March in male rhesus monkeys otherwise, it is possible that the naturally occurring reproductive quiscence may be attributed to the effect of the drug being tested. The data accrued from the present studies also provide quantitative information on circulating levels of testosterone which could be used as a reference background while evaluating the contraceptive drug-effects in male rhesus monkeys

Development of a water-based cooling system for the Muon Chamber detector system of the CBM experiment

A water-based cooling system is being investigated to meet the cooling requirement of the Gas Electron Multiplier (GEM) based Muon Chamber (MuCh) detector system of the Compressed Baryonic Matter (CBM) experiment at GSI, Germany. The system is based on circulating cold water through the channels inside an aluminium plate. The aluminium plate is attached to a GEM chamber. A feasibility study is conducted on one small and two real-size prototype cooling plates. A microcontroller based unit has been built and integrated into the system to achieve automatic control and monitoring of temperature on plate surface. The real-size prototypes have been used in a test beam experiment at the CERN SPS (Super Proton Synchrotron) with the lead beam on a lead target. A setup using three prototype modules has been prepared in the lab for testing in a simulated real life environment. This paper discusses the working principle, mechanical design, fabrication, and test results of the cooling prototypes in detail.Comment: 8 pages, 12 figures, 2 table

Fingerprinting of Sahaj Vati, additive for natural lovastatin production

The lovastatin has been used in hypercholesterolemia and heart diseases for therapeutic as well as preventative purposes and it is naturally obtained from filamentous fungi such as Aspergillus through biotechnological approach. The additives in fungal media which enhances the fungal growth should also increase the production of lovastatin. Finger printing of Sahaj Vati, a herbo-mineral formulation comprising mixture of Plumbago zeylanica, Curcuma longa, Shilajeet, Commiphora mukul and Clerodendrum phlomidis powder to be added in medium for fungal growth. The fungal growth activity of both batches of Sahaj Vati was tested for fungal isolate of A. flavus by contact measure through hyphal development restraint test utilizing Potato dextrose agar (PDA) and SMKY medium followed by chromatographic and different techniques for standardization. Sahaj Vati has lower antifungal index & increased the fungal biomass and separation of compound was higher in mobile phase Benzene: Ethyl acetate: Pyridine: 5:4.9:0.1 & Hexane: Chloroform: Pyridine: 2.7:1. Sahaj Vati increased the growth and biomass of A. flauvs which may be used for natural production of lovastatin

High space-bandwidth in quantitative phase imaging using partially spatially coherent optical coherence microscopy and deep neural network

Quantitative phase microscopy (QPM) is a label-free technique that enables to monitor morphological changes at subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence properties of the light source and the numerical aperture (NA) of objective lenses. Here, we propose high space-bandwidth QPM using partially spatially coherent optical coherence microscopy (PSC-OCM) assisted with deep neural network. The PSC source synthesized to improve the spatial sensitivity of the reconstructed phase map from the interferometric images. Further, compatible generative adversarial network (GAN) is used and trained with paired low-resolution (LR) and high-resolution (HR) datasets acquired from PSC-OCM system. The training of the network is performed on two different types of samples i.e. mostly homogenous human red blood cells (RBC) and on highly heterogenous macrophages. The performance is evaluated by predicting the HR images from the datasets captured with low NA lens and compared with the actual HR phase images. An improvement of 9 times in space-bandwidth product is demonstrated for both RBC and macrophages datasets. We believe that the PSC-OCM+GAN approach would be applicable in single-shot label free tissue imaging, disease classification and other high-resolution tomography applications by utilizing the longitudinal spatial coherence properties of the light source