<i>Trixis angustifolia</i> hexanic extract displays synergistic antibacterial activity against <i>M. tuberculosis</i>

<p>A phytochemical and antibacterial study of <i>Trixis</i> <i>angustifolia</i>, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against <i>Mycobacterium tuberculosis</i> H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against <i>M. tuberculosis</i> (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in <i>T. angustifolia</i> is due to the combined action of diverse metabolites present in the plant.</p