First releases of hatchery‑produced Senegal sole (Solea senegalensis), brill (Scophthalmus rhombus), and wedge sole (Dicologoglossa cuneata) juveniles in the South‑western Spanish coast

Primeras y únicas repoblaciones con peces en el litoral de HuelvaThe regression of fisheries in the Gulf of Cadiz is evident since current fish catches are 33% of that 30 years before. Consequently, some initiatives for the replenishment of exhausted wild stocks are welcome. The objective of the present work is to describe and analyze the results coming from the first flatfish stock enhancements in Andalusia. A total of 3189 fish from three flatfish species: Senegal sole (Solea senegalensis Kaup), wedge sole (Dicologlossa cuneata Moreau), and brill (Scophthalmus rhombus Linnaeus) were tagged and released. Several variables were calculated through the data analysis of recovered fish. Some variables were calculated only for Senegal soles since wedge sole and brill recaptures were not significant. The Senegal sole recapture rate was 2.71±0.72, similar to other published data, the recapture rates for bigger fish being higher though not significant. No significant differences were detected for distance, time, growth or recapture rate among initial Senegal sole sizes. Around 80% of recaptures were registered within 15 weeks after release. The results show that it is possible the release and recovery of tagged Senegal soles in the Gulf of Cadiz. Future long-term programs on stock enhancement could help to determine the effects on fisheries and recover stocks.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study has been financed by the Consejería de Agricultura, Ganadería, Pesca y Desarrollo Sostenible of the Junta de Andalucia. M. Herrera’s post-doc contract is supported by the National Institute of Agricultural Research and the European Social Fund (INIA-FSE). Funding for open access charge: Universidad de Huelva / CBUA

Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome

A study was carried out to investigate the combined effect of exogenous enzymes and probiotic supplementation on tilapia growth, intestinal morphology and microbiome composition. Tilapia (34.56 ± 0.05 g) were fed one of four diets (35% protein, 5% lipid); one of which was a control and the remaining three diets were supplemented with either enzymes (containing phytase, protease and xylanase), probiotic (containing Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus) or enz-pro (the combination of the enzymes and probiotic). Tilapia fed diet supplemented with enz-pro performed better (P < 0.05) than tilapia fed the control and probiotic supplemented diets in terms of final body weight (FBW), specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER). The dietary treatments did not affect somatic indices. The serum lysozyme activity was significantly higher (P < 0.05) in tilapia fed the probiotic supplemented diet than of those fed the remaining experimental diets. The intestinal perimeter ratio was higher (P < 0.05) in tilapia fed enz-pro supplemented diet when compared to those fed with the control and probiotic supplemented diets. Goblet cells abundance, microvilli diameter and total enterocyte absorptive surface was higher (P < 0.05) in tilapia fed diet supplemented with enz-pro than those fed the control diet. High-throughput sequencing revealed that majority of reads derived from the tilapia digesta belonged to members of Fusobacteria (predominantly Cetobacterium) distantly followed by Proteobacteria and Firmicutes. The alpha and beta diversities did not differ among dietary treatments indicating that the overall microbial community was not modified to a large extent by dietary treatment. In conclusion, supplementation of the diet with a combination of enzymes and probiotic is capable of improving tilapia growth and intestinal morphology without deleterious effect on the intestinal microbial composition

Handbook on the culture of brill (Scophthalmus rhombus)

Guía sobre los distintos aspectos a tener en cuenta para llevar a cabo el cultivo del parracho en base a las diferentes fases de su cultivo: captura, adaptación y mantenimiento de reproductores, obtención de puestas, cultivo larvario y engorde. Además, incluye un análisis sensorial de la especie en el que se detalla un estudio descriptivo de las propiedades organolépticas de los peces cultivados y un estudio de aceptación del parracho por parte del consumidor

Composition and evolution of the gut microbiota of growing puppies is impacted by their birth weight

Abstract Low birth weight puppies present an increased risk of neonatal mortality, morbidity, and some long-term health issues. Yet it has not been investigated if those alterations could be linked to the gut microbiota composition and evolution. 57 puppies were weighed at birth and rectal swabs were performed at 5 time points from birth to 28 days of age. Puppies were grouped into three groups based on their birth weight: low birth weight (LBW), normal birth weight (NBW) and high birth weight (HBW). 16S rRNA gene sequencing was used to highlight differences in the fecal microbiota. During the first three weeks, the relative abundance of facultative anaerobic bacteria such as E. coli, C. perfringens and Tyzzerella was higher in LBW feces, but they catch back with the other groups afterwards. HBW puppies showed higher abundances of Faecalibacterium and Bacteroides during the neonatal period, suggesting an earlier maturation of their microbiota. The results of this study suggest that birth weight impact the initial establishment of the gut microbiota in puppies. Innovative strategies would be desired to deal with altered gut microbiota in low birth weight puppies aiming to improve their survival and long term health

Aquaculture developments and cross-border cooperation in IFAPA

This documentary seeks to highlight the technological improvements in infrastructure and laboratories and scientific advances made within ECOAQUA project, in IFAPA ¿Agua del Pino¿Este documental pretende dar a conocer las mejoras tecnológicas en las infraestructuras y laboratorios y los avances científicos logrados en el Centro IFAPA Agua del Pino en el seno de proyecto ECOAQU

Positive biofilms to control surface-associated microbial communities in a broiler chicken production system - a field study

International audienceIn the One Health concept, the use of beneficial bacteria to form positive biofilms that prevent the settlement of undesirable bacteria is a promising solution to limit the use of antimicrobials on farms. However, there is a lack of field studies reporting the onset of these beneficial bacteria after application and the effects on autochthonous surface microbiota. In the study reported here, the inner surfaces of commercial broiler chicken houses were treated or not with a bacterial consortium composed of Bacillus spp. and Pediococcus spp. strains, able to form covering biofilms in different laboratory models. Preinstalled coupons were sampled over time to capture microbial biofilm dynamics on-farm surfaces. The results showed that the bacterial consortium can establish on the farm surfaces, modulate microbial communities, and limit the implantation of Enterobacteriaceae and Enterococcaceae, two families containing potential pathogens