Ecoliteracia: atividades para interrogar o mundo com crianças do 1.º e 2.º Ciclo do Ensino Básico

Relatório de estágio de mestrado em Ensino do 1.º e 2.º Ciclo do Ensino BásicoO presente relatório relata a intervenção implementada no 1º e no 2º ciclo do Ensino Básico, no âmbito da disciplina de Prática de Ensino Supervisionada (PES), do 2.º ano de Mestrado em Ensino do 1.º e 2.º Ciclo do Ensino Básico, tendo o eixo de ação sido a análise de uma obra literária adequada às faixas etárias envolvidas e aos conteúdos programáticos então lecionados. Incidente sobre a temática da educação para a cidadania através do conceito de Ecoliteracia, esta abordagem centrou-se na interdisciplinaridade, atualmente em voga no contexto pedagógico. Procurando então explorar questões relacionadas com a ecologia e com a sustentabilidade ambiental, e partindo da Língua Portuguesa, procurámos articular conhecimentos, não apenas encarando a literatura como um excelente veículo para ver cumprido um dos maiores desafios que se encontram na escola na atualidade - a diversidade -, mas também versando sobre outras áreas do saber como a Matemática, o Estudo do Meio e até as Artes. Cremos, assim, que, através da interdisciplinaridade, poderíamos apelar de forma mais efetiva e comprometida à reflexão e à construção social do indivíduo, de forma a torná-lo ativo face ao seu futuro e ao dos demais. A amostra de participantes era constituída por 11 alunos do 3.º ano e 14 do 4.º ano, e ainda de 22 alunos de uma turma do 5.º ano, o que perfaz um total de 47 alunos. Baseado numa metodologia do tipo qualitativo, este estudo procurou valorizar o público-alvo, pelo que as estratégias foram delineadas de acordo com o seu contexto educativo. Desta forma, a experiência permitiu concluir que, encarando as crianças de hoje como adultos de amanhã, cabe ao professor observar, planificar, refletir e avaliar as práticas dos seus alunos, de forma a melhorar, por um lado, as próprias práticas de ensino, e por outro a formar cidadãos.This report describes the intervention carried out in the 1st and 2nd cycle of Basic Education, under the subject Supervised Teaching Practice, which is included in the second year of Master's Degree in Teaching of the 1st and 2nd Cycle. The study focuses on the analysis of a literary work appropriate to the participants' age and to the taught contents. The research concerns on the theme of citizenship education through the concept of Ecoliteracy, so the approach focused on interdisciplinarity, currently in vogue in the pedagogical context. In order to explore issues related to ecology and environmental sustainability, and starting from the Portuguese language, we wanted to articulate students' knowledge, not only looking at literature as an excellent vehicle to fill one of the greatest challenges at school - diversity -, but also dealing with other areas of knowledge such as Mathematics, Science and even Arts. We believe that, through interdisciplinarity, we could appeal to a more effective and committed way of reflection and social construction, in order to make everyone active in relation to our future and the others' too. The sample of participants consisted of 11 students of the 3rd year and 14 of the 4th year, and also of 22 students of a 5th year class, which makes a total of 47 students. Based on a qualitative methodology, this study tried to value the target audience, so the strategies were delineated according to their educational context. Thus the experience allowed us to conclude that considering today's children as tomorrow's adults, it is up to the teacher to observe, plan, reflect and evaluate his students' practices in order to improve his or her own teaching practices and to raise citizens

Lymphogranuloma venereum: a retrospective analysis of an emerging sexually transmitted disease in a Lisbon Tertiary Center

Commentary: G. Ciccarese et al. J Eur Acad Dermatol Venereol 2021; 35:1606-1607. https://doi.org/10.1111/jdv.17468.Background: Lymphogranuloma venereum (LGV) is a sexual transmitted infection (STI), currently endemic within the population of men who have sex with men (MSM) of Western Countries. L2B variant has been reported as the predominant strain in the current LGV epidemics, although a shift towards L2-434 has been observed in some European countries. Objectives: To evaluate and characterize the population with LGV infection diagnosed in Centro Hospitalar Universitário de Lisboa Central, Lisbon, Portugal. Methods: A retrospective analysis of all LGV diagnoses between 2016 and 2019 was performed. The diagnosis was established through ompA-genotyping of samples yielding a positive result to Chlamydia trachomatis (CT). All considered samples were retrieved from the clinician activity, through swabbing and urine analysis and CT infection diagnosis was obtained using real-time PCR. Results: During the period studied 16 279 CT diagnostics tests were employed, with a striking increase from 2016 (n = 467) to 2019 (n = 9362). A total of 1602 diagnoses of CT were established, from which 168 (10.5%) corresponded to LGV, with both infections showing a rising evolution, between 2016 and 2019, of 2.9 and 2.7 times, respectively. The majority of the LGV strains were genotyped as L2/434 (67.3%; n = 113). LGV predominantly affected MSM and men who have sex with men and women (97.0%; n = 163). Anorectal infection was the most prevalent one (90.5%; n = 152), being proctitis the main clinical presentation (76.2%; n = 128). Absence of symptoms was reported in almost 15% of the cases (n = 24). The presence of concomitant infection with human immunodeficiency virus was dominant (73.2%; n = 123) and the prevalence of one or more STI co-infections was about 60.1% (n = 99). Conclusions: An increasing evolution of CT and LGV testing and diagnosing was observable throughout the studied period. Characteristics of the population are similar with those described within LGV epidemics. In accordance with recent European studies, predominance towards L2 genotype was identified.info:eu-repo/semantics/publishedVersio

Migrants and their Descendants: Social Inclusion and Participation in Society

Country studie

The effects of an intervention on emotions using traditional stories

Este trabalho teve dois objetivos a elaboração de um programa para trabalhar emoções básicas com crianças em idade pré-escolar sustentado em histórias infantis e a análise do seu impacto no desenvolvimento do conhecimento das emoções a 3 níveis: nomeação, reconhecimento e conhecimento da causa das emoções, O programa foi desenvolvido com crianças dos 3 aos 5 anos, existindo um grupo de intervenção com questionamento ao nível das emoções (Alegria, Tristeza, Raiva e Medo) e um grupo de controlo. Após 8 sessões identificaram-se diferenças estatisticamente significativas entre os 2 grupos, no seu conhecimento das emoções.This research had two aims: the organization of a program to work basic emotions with preschool age children based on children’s stories and the analyses of its impact on the development of children’s knowledge about emotions at three levels: nomination, recognition and knowledge about emotions’ causes. The program was developed with children from 3 to 5 years old, with an intervention group questioned about emotions (Joy, Sadness, Anger and Fear) and a control group. After 8 sessions, statistically significant differences were identified between the 2 groups in their knowledge about emotions.info:eu-repo/semantics/publishedVersio

Hydrogeochemistry and distribution of potentially toxic elements in a metallogenic province – The Iberian Pyrite Belt, Portugal

Contamination by acid drainage is an environmental problem in mineralized regions, especially in the surroundings of sulphide mines. The water rock interaction process involves the oxidative dissolution of sulphides, naturally or by mining activity, that generates acidity which, in turn, produces sulfate, and water pollution by sulphide-hosted metals. The particular geology of the Iberian Pyrite Belt (IPB) in the southwestern Iberian Peninsula creates the ideal conditions for such water contamination. Different water types were sampled at 28 locations across the entire IBP metallogenetic province. Anions, metals, and arsenic were analysed to assess the influence of acid drainage. The results demonstrate the sulfate nature of waters in the vicinity of mines. Other types of water in the IPB region are mainly mixed chloride and bicarbonate (river and groundwater) and mixed and sodium-bicarbonate (lakes) types. Water quality assessment indicated strong contamination of surface waters that are directly influenced by mine wastes. There is the additional concern that some lakes and groundwaters exhibit concentrations of potentially toxic elements (e.g. Al, As) that are above the regulatory limits established by the European Commission Water Framework Directive.This work was co-funded by the European Union through the European Regional Development Fund, based on COMPETE 2020 - project ICT (UID/GEO/04683/2013) with reference POCI-01-0145-FEDER-007690 and project Nano-MINENV number 029259

International consensus on antinuclear antibody patterns in Portugal

Introduction: Screening for autoantibodies in HEp-2 cells by indirect immunofluorescence is currently accepted as the gold-standard test for the diagnosis of systemic autoimmune diseases. The main objective of the International Consensus on ANA Patterns is to achieve a consensus on the nomenclature and description of antinuclear antibody morphological patterns. This work aims to build on the International Consensus on ANA Patterns project to establish a nomenclature consensus in Portugal, thus contributing to harmonization in autoimmune diagnosis and promoting diagnostic quality in autoimmune systemic rheumatic diseases. Material and Methods: Participating laboratories identified all the nuclear and cytoplasmic pattern designations in the International Consensus on ANA Patterns (including the anti-cell pattern code), and matched them with the corresponding Portuguese nomenclature in use. The results were aggregated and used as a foundation for nomenclature harmonization work. Consensus meetings followed an iterative process, until a final consensual proposal was drafted. Results: Prior agreement between laboratories was over 75% for 23 of the total 29 anti-cell patterns. The degree to which each laboratory is aligned with the International Consensus on ANA Patterns international reference ranges from 22.1% to 100%. It was possible to write a consensual version of the International Consensus on ANA Patterns nomenclature for Portugal. Discussion: There was a good consensus basis for the nomenclature in the International Consensus on ANA Patterns, despite relevant differences with some translations. The study highlights the need for collaboration among laboratories towards an unambiguous description of laboratory results. Conclusion: This study shows that there is good potential for collaboration between laboratories in order to produce the consensus needed to improve diagnosis and patient follow-up.publishersversionpublishe

Host Glycan Sugar-Specific Pathways in Streptococcus pneumonia:Galactose as a Key Sugar in Colonisation and Infection

The human pathogen Streptococcus pneumoniae is a strictly fermentative organism that relies on glycolytic metabolism to obtain energy. In the human nasopharynx S. pneumoniae encounters glycoconjugates composed of a variety of monosaccharides, which can potentially be used as nutrients once depolymerized by glycosidases. Therefore, it is reasonable to hypothesise that the pneumococcus would rely on these glycan-derived sugars to grow. Here, we identified the sugar-specific catabolic pathways used by S. pneumoniae during growth on mucin. Transcriptome analysis of cells grown on mucin showed specific upregulation of genes likely to be involved in deglycosylation, transport and catabolism of galactose, mannose and N acetylglucosamine. In contrast to growth on mannose and N-acetylglucosamine, S. pneumoniae grown on galactose re-route their metabolic pathway from homolactic fermentation to a truly mixed acid fermentation regime. By measuring intracellular metabolites, enzymatic activities and mutant analysis, we provide an accurate map of the biochemical pathways for galactose, mannose and N-acetylglucosamine catabolism in S. pneumoniae. Intranasal mouse infection models of pneumococcal colonisation and disease showed that only mutants in galactose catabolic genes were attenuated. Our data pinpoint galactose as a key nutrient for growth in the respiratory tract and highlights the importance of central carbon metabolism for pneumococcal pathogenesis