Breast implant-associated anaplastic large cell lymphoma: a diagnostic challenge

Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) is a provisional entity with morphological and immunophenotypic characteristics indistinguishable from ALK-negative anaplastic large cell lymphoma (ALCL). Unlike ALCL, BIA-ALCL arises mainly in association with breast implantation. Diagnostic confirmation of BIA-ALCL can be difficult and associating morphological and pathological hallmarks with flow cytometry and immunohistochemistry can assist in the diagnosis. The objective of this report is to describe a case of BIA-ALCL in which cytological and immunophenotypological analysis using flow cytometry suggested the presence of large CD30-positive cells in the effusion fluid

Physico-chemical characterization of asolectin–genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability

AbstractIn this study, the interaction between soy isoflavone genistein and asolectin liposomes was investigated by monitoring the effects of isoflavone on lipidic hydration, mobility, location and order. These properties were analyzed by the following techniques: horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), low-field 1H nuclear magnetic resonance (NMR), high-field 31P NMR, zeta potential, differential scanning calorimetry (DSC) and UV–vis spectroscopy. The antioxidant and antitumoral activities of the genistein liposomal system were also studied. The genistein saturation concentration in ASO liposomes corresponded to 484μM. HATR–FTIR results indicated that genistein influences the dynamics of the lipidic phosphate, choline, carbonyl and acyl chain methylenes groups. At the lipid polar head, HATR–FTIR and 31P NMR results showed that the isoflavone reduces the hydration degree of the phosphate group, as well as its mobility. Genistein ordered the lipid interfacial carbonyl group, as evidenced by the HATR–FTIR bandwidth analysis. This ordering effect was also observed in the lipidic hydrophobic region, by HATR–FTIR, NMR, DSC and turbidity responses. At the saturation concentration, liposome-loaded genistein inhibits the lipid peroxidation induced by hydroxyl radical in 90.9%. ASO liposome-loaded genistein at 100μM decreased C6 glioma cell viability by 57% after 72h of treatment. Results showed an increase of the genistein in vitro activities after its incorporation in liposomes. The data described in this work will contribute to a better understanding of the interaction between genistein and a natural-source membrane and of its influence on isoflavone biological activities. Furthermore, the antitumoral results showed that genistein-based liposomes, which contain natural-sourced lipids, may be promising as a drug delivery system to be used in the glioma therapy

Immunoexpression of PD-L1, CD4+ and CD8+ cell infiltrates and tumor-infiltrating lymphocytes (TILs) in the microenvironment of actinic cheilitis and lower lip squamous cell carcinoma

Lower lip squamous cell carcinomas (LLSCC) could be associated with a previous history of potentially malignant oral diseases (PMOD), especially actinic cheilitis (AC), with high sun exposure being a well-described risk factor. Immune evasion mechanisms, such as the PD-1/PD-L1 (programmed cell death protein 1/programmed death-ligand 1) pathway has been gaining prominence since immunotherapy with immune checkpoint inhibitors showed a positive effect on the survival of patients with different types of neoplasms. Concomitant with the characterization of the tumor microenvironment, the expression of either or both PD-1 and PD-L1 molecules may estimate mutual relations of progression or regression of the carcinoma and prognostic values of the patient. Objective: Considering the importance of tumor microenvironment characterization, this study aims to determine the immunoexpression of PD-L1 and correlate with the frequency of CD4+ and CD8+ cells in AC and LLSCC lesions and with tumor-infiltrating lymphocytes (TILs) in LLSCC and its relationship with histopathological characteristics. Methodology: This sample includes 33 cases of AC and 17 cases of LLSCC. The cases were submitted to histopathological analysis and to CD4+, CD8+, and PD-L1+ cell determination by immunohistochemistry. Results: There was a significant difference among the frequencies of CD4+, CD8+, and PD-L1+ cells between AC and LSCC cases, higher in the last group. Moreover, histopathological and atypical changes in AC and LLSCC were correlated with the frequencies of PD-L1+, CD4+, and CD8+ cells. In AC, PD-L1+ cases had a low frequency of CD4+ cells, but on the other hand, PD-L1+ cases of LLSCC had a higher frequency of CD4+ and CD8+ cells. Conclusion: Therefore, the PD-L1 molecule may be a potential escape route for the immune response in oral lesions, but the mechanisms differ between AC and LLSCC. Future studies related to immune evasion and immunotherapy in oral lesions should consider the analysis of inflammatory infiltrate and TILs

implication for assessing the risk of arbovirus outbreaks

Funding Information: This work was also supported by the U.S. Centers for Disease Control and Prevention through a cooperative agreement number 5NU14GH001237-03-00. The views expressed in this written publication do not necessarily reflect the official policies of the U.S. Department of Health and Human Services. European Foundation Initiative into Neglected Tropical Disease also supported this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank all community leaders, household heads staff from the provincial health directorates, staff from the Medical Entomology Laboratory from the National Institute of Health, staff from National Malaria Control Programme, and staff from the Laboratório de Transmissores de Hematozoários of the Oswaldo Cruz Institute for their support during field work, laboratory testing and identification of Aedes spp. We also thank José Feriano Américo who produced the maps of this work. Ours special thanks goes to Professor J. Dereck Charlwood who revised the English grammar and typos of the entire manuscript. Consent for publication Our manuscript does not present any individual person's data. Publisher Copyright: © 2018 Abílio et al. http://creativecommons.org/licenses/by/4.0/.Background: Aedes-borne arboviruses have emerged as an important public health problem worldwide and, in Mozambique, the number of cases and its geographical spread have been growing. However, information on the occurrence, distribution and ecology of Aedes aegypti and Ae. albopictus mosquitoes remain poorly known in the country. Methods: Between March and April 2016, a cross-sectional study was conducted in 32 districts in Mozambique to determine the distribution and breeding sites of Ae. aegypti and Ae. albopictus. Larvae and pupae were collected from a total of 2,807 water-holding containers using pipette, dipper, funnel and sweeping procedures, depending on the container type and location. Both outdoor and indoor water-holding containers were inspected. The immature forms were reared to adults and the identifications of the mosquito species was carried out with a stereomicroscope using a taxonomic key. Results: Aedes aegypti was found in every district sampled, while Ae. albopictus was only found in Moatize district, situated in Tete Province in the central part of the country. Six hundred and twenty-eight of 2,807 (22.4%) containers were positive for Ae. aegypti but only one (0.03%) was positive for Ae. albopictus. The Container Index (CI) of Aedes was highest in densely populated suburban areas of the central region (260/604; 43.0%), followed by suburban areas in northern areas (228/617; 36.9%) whilst the lowest proportion was found in urbanized southern areas (140/1586; 8.8%). The highest CI of Aedes was found in used tires (448/1268; 35.3%), cement tanks (20/62; 32.3%) and drums (21/95; 22.1%). Conclusion: Data from our study showed that Ae. aegypti is present nation-wide, since it occurred in every sampled district, whilst Ae. albopictus had a limited distribution. Therefore, the risk of transmission of dengue and chikungunya is likely to have been underestimated in Mozambique. This study highlights the need for the establishment of a national entomological surveillance program for Aedes spp. in Mozambique in order to gain a better understanding about vector bionomics and to support the development of informed effective vector control strategies.publishersversionpublishe

Características clínicas e laboratoriais de pacientes que apresentam clone de hemoglobinúria paroxística noturna detectado por citometria de fluxo

Resumo: INTRODUÇÃO: A Hemoglobinúria Paroxística Noturna (HPN) é caracterizada clinicamente por hemólise intravascular crônica, falência medular e hipercoagulabilidade levando a tromboses. A facilidade na detecção de clone HPN através do uso de técnicas sensíveis aumentou o número de pacientes com este diagnóstico, entretanto, a heterogeneidade da doença torna imprescindível a correlação clínica. OBJETIVOS: Analisar as características clínico-biológicas de uma população brasileira de pacientes com clone HPN, e avaliar as diferenças na apresentação, sobrevida global, diferença entre as categorias clínicas e significado do clone HPN. MÉTODOS: 103 pacientes foram selecionados através de uma busca nos exames de imunofenotipagem por citometria de fluxo (CF) realizados entre dezembro de 1999 e dezembro de 2011, no Laboratório de Imunofenotipagem, HC-UFPR. Definição de Clone HPN+: CF com defeito de expressão >0,1% em pelo menos dois marcadores (CD55, CD59, CD16, CD24, CD66b, CD14) em duas linhagens hematopoéticas distintas. Análise retrospectiva foi realizada nos pacientes que tinham prontuário clínico com pelo menos uma consulta avaliável, feita no ambulatório de Falência Medular HC-UFPR. RESULTADOS: 103 pacientes incluídos no estudo, 52M / 51F, idade mediana 24,1 anos (5.5-62). Sintomas iniciais: astenia 95,1%; infecção 46,6%; sangramentos 66%, sendo 47,1% petéquias e equimoses; hemoglobinúria 23.2%; dor abdominal 38,8%; icterícia 13,6%. Trombose foi observada em 17 (16,5%) pacientes. Citopenias estavam presentes em 101 pacientes: 83(80,6%) pancitopenia, 12(15,2%) anemia e trombocitopenia, e 4(3,9%) anemia e leucopenia. A mediana de hemoglobina foi 8,8g/dL (3,8-14,5 g/dL), neutrófilos 940×103/?L (260-1.450×103/?L), e plaquetas 25×106/?L (2-294×106/?L). Os pacientes foram divididos em 3 grupos, conforme a celularidade da medula óssea e presença de hemólise: 10 pacientes HPN clássica, 39 pacientes HPN + SFM e 54 pacientes HPN subclínica. A celularidade mediana da medula óssea foi 5% em HPN subclínica, 10% na HPN+SFM e 70% na HPN clássica. Vinte e seis pacientes desenvolveram hemólise após uma mediana de 2,35 anos após o diagnóstico da aplasia. A sobrevida global em 10 anos estimada pela curva de Kaplan-Meier foi 81,7% no grupo todo; o grupo HPN subclínica apresentou menor SG (76,5%). Houve diferenças significativas nos sintomas clínicos, leucócitos, plaquetas, DHL, reticulócitos e tamanho do clone HPN entre as três subcategorias, especialmente entre HPN clássica e subclínica. As percentagens de clone HPN em eritrócitos e granulócitos foram significativamente diferentes entre os três grupos (p<0.001), com percentagens medianas respectivamente: 0,04% (0-10%) e 7.3% (6,4-19,0%) em HPN subclínica; 15,8% (14-52%) e 63% (56-78%) em HPN+SFM, e 82,2% (89,7-92,2%) e 98,0% (86,7-98,4%) na HPN clássica. Também houve diferença estatística entre os pacientes com eventos trombóticos ou não nas variáveis: plaquetas (p=0,001), DHL (p=0,002), mediana do clone HPN em neutrófilos=92,7% (3.4%-100%) versus 21,5% (0,25%-99,9%), p<0,001, e eritrócitos =31,8% (0-100%) versus 1,2% (0-100%), p=0,008. CONCLUSÕES: Esta análise retrospectiva representa a maior coorte de pacientes com clone HPN descrita em um único centro no Brasil, e a primeira descrição dos subgrupos HPN clássica, HPN associada à falência medular e HPN subclínica. Os resultados sugerem que pacientes com clones de tamanho grande tem maior incidência de hemólise e trombose, mesmo quando no cenário das síndromes de falência medular. As três categorias clínicas podem ser diferenciadas não só pela biópsia de medula óssea e presença de hemólise documentada, mas também pelo tamanho do clone HPN. Os resultados da citometria de fluxo em pacientes com clone HPN devem ser correlacionados com as informações clínicas e laboratoriais para o correto diagnóstico, classificação e tratamento

A bibliometria nos periódicos de ciência da Informação no Brasil

Neste artigo apresentam-se os resultados de uma pesquisa que objetivou analisar quantitativamente a abordagem do método bibliométrico e também sua utilização. Os itens de análise foram artigos publicados em periódicos nacionais na área de Ciência da Informação do ano 2006 a 2011. Os objetivos são verificar o número de publicações por ano, o número de artigos publicados por cada revista, o número de autores por artigo, a titulação dos autores, a instituição com a qual os autores possuem vinculo, o tipo de pesquisa, o tema de pesquisa, a região que mais publica e os autores que mais produzem em bibliometria. Conclui-se que a Bibliometria vem sendo utilizada em todo Brasil, mais frequentemente na região sudeste. Os profissionais de todas as áreas podem e estão utilizando esta técnica para assuntos relacionados à sua realidade. Acredita-se que a pesquisa mostrou resultados satisfatórios, pois verificou-se que a bibliometria está em crescimento, tanto com trabalhos que tratam especificamente sobre ela ou que dela se utilizam para averiguar crescimento de produção sobre determinado assunto. É importante salientar a contribuição de profissionais com pós-graduação no exterior, trazendo técnicas novas e contribuindo para que o nosso nível de profissionais e pesquisadores seja cada vez melhor.This study aims to show the results of a research based on the analysis of the bibliometric method approach and also its utilization. The items of the analysis were published articles in national periodics in Information Science of 2006 to 2011. The goals are the verification of the number concerning: publications by year, published articles by magazine, and authors by article. It will be also verified the authors’ title, the institution in which the authors are included, the type of the research, the research theme, the most publishing region, and the most producing authors in bibliometrics. It is concluded that Bilbiometric is used all over Brazil, especially in Southeast. The professional from all areas are able and are using this technique regarding issues related to their reality. It is believed that the research has shown satisfactory results, because it was verified that bibliometrics is in developing process, such as the projects about it or those ones that used it to know the production growth about a specific subject. It is also important to highlight the contribution of the postgraduate abroad professionals, who 20are bringing new techniques and also contributing in order to increase the standard of professionals and researchers

Circunferência abdominal como preditor de evolução em 30 dias na síndrome coronariana aguda

FUNDAMENTO: A circunferência abdominal (CA) é a medida que mais se correlaciona com os fatores de risco e morte por doença cardiovascular. Entretanto, o impacto da obesidade no prognóstico de pacientes com doenças cardiovasculares permanece controverso e requer maiores esclarecimentos. OBJETIVO: Avaliar a CA como preditor de evolução em 30 dias em pacientes que internaram com síndrome coronariana aguda (SCA), em hospital de referência no tratamento de doenças cardiovasculares. MÉTODOS: Coorte contemporânea com 267 pacientes que internaram por SCA e que foram seguidos por 30 dias após a alta levando em consideração os eventos cardiovasculares maiores - MACE - (óbito, reinfarto, reinternação para procedimentos de revascularização). Nas primeiras 24 horas da admissão, os pacientes responderam a um questionário e posteriormente tiveram a CA mensurada. A análise estatística foi realizada com SPSS 17.0, utilizando o teste do Qui-quadrado para variáveis categóricas e o teste t de Student para as variáveis numéricas, com o nível de significância de p < 0,05. As variáveis que apresentaram valores de p < 0,10, na análise bivariada, foram incluídas em um modelo de regressão logística para avaliar o papel da CA como preditor independente de MACE. RESULTADOS: Após análise multivariável, apenas o gênero feminino (RC = 8,86; 95% IC:4,55-17,10; p < 0,00), hipertensão arterial sistêmica (RC = 2,06; 95% IC:1,10-3,87; p = 0,02) e história familiar de cardiopatia isquêmica (RC = 2,10; 95% IC:1,17-3,74; p = 0,01) permaneceram associados com os MACE. CONCLUSÃO: Em nosso estudo, a CA alterada não se associou à maior incidência de MACE em 30 dias de seguimento