Cascading Crypthecodinium cohnii biorefinery: global warming potential and techno-economic assessment

ABSTRACT: Prior to the commissioning of a new industrial biorefinery it is deemed necessary to evaluate if the new project will be beneficial or detrimental to climate change, one of the main drivers for the sustainable development goals (SDG) of the United Nations. In particular, how SDG 7, Clean and Efficient Energy, SDG 3, Good Health and Well Being, SDG 9, Industry Innovation and Infrastructure, and SDG 12, Responsible Production and Consumption, would engage in a new biorefinery design, beneficial to climate change, i.e., fostering SDG 13, Climate Action. This study uses life cycle assessment methodology (LCA) to delve in detail into the Global Warming Impact category, project scenario GHG savings, using a conventional and a dynamic emission flux approach until 2060 (30-year lifetime). Water, heat and electricity circularity are in place by using a water recirculation process and a combined heat and power unit (CHP). A new historical approach to derive low and higher-end commodity prices (chemicals, electricity, heat, jet/maritime fuel, DHA, N-fertilizer) is used for the calculation of the economic indicators: Return of investment (ROI) and inflation-adjusted return (IAR), based upon the consumer price index (CPI). Main conclusions are: supercritical fluid extraction is the hotspot of energy consumption; C. cohnii bio-oil without DHA has higher sulfur concentration than crude oil based jet fuel requiring desulfurization, however the sulfur levels are compatible with maritime fuels; starting its operation in 2030, by 2100 an overall GHG savings of 73% (conventional LCA approach) or 85% (dynamic LCA approach) is projected; economic feasibility for oil productivity and content of 0.14 g/L/h and 27% (w/w) oil content, respectively (of which 31% is DHA), occurs for DHA-cost 100 times higher than reference fish oil based DHA; however future genetic engineering achieving 0.4 g/L/h and 70% (w/w) oil content (of which 31% is DHA), reduces the threshold to 20 times higher cost than reference fish oil based DHA; N-fertilizer, district heating and jet fuel may have similar values then their fossil counterparts.info:eu-repo/semantics/publishedVersio

The Biorefinery of the Marine Microalga Crypthecodinium cohnii as a Strategy to Valorize Microalgal Oil Fractions

ABSTRACT: Chrypthecodinium cohnii lipids have been almost exclusively used as a source of Docosahexaenoic acid (DHA). Such an approach wastes the remaining microalgal lipid fraction. The present work presents a novel process to produce C. cohnii biomass, using low-cost industrial by-products (raw glycerol and corn steep liquor), in a 7L-bioreactor, under fed-batch regime. At the end of the fermentation, the biomass concentration reached 9.2 g/L and the lipid content and lipid average productivity attained 28.0% (w/w dry cell weight) and 13.6 mg/L h, respectively. Afterwards the microalgal biomass underwent a saponification reaction to produce fatty acid (FA) soaps, which were further converted into FA ethyl ester (FA EE). C. cohnii FA EE mixture was then fractionated, using the urea complexation method at different temperatures, in order to obtain a polyunsaturated fatty acid ethyl ester (PUFA EE) rich fraction, that could be used for food/pharmaceutical/cosmetic purposes, and a saturated fatty acid ethyl ester (SAT EE) rich fraction, which could be used as biodiesel. The temperature that promoted the best separation between PUFA and SAT EE, was -18 degrees C, resulting in a liquid fraction with 91.6% (w/w) DHA, and a solid phase with 88.2% of SAT and monounsaturated fatty acid ethyl ester (MONOUNSAT), which could be used for biodiesel purposes after a hydrogenation step.info:eu-repo/semantics/publishedVersio

Efeito da adição de estabilizantes UV no comportamento reológico de resinas epóxi e poliéster

O uso de agentes estabilizantes contra a radiação Ultravioleta é essencial para manutenção da integridade das resinas poliméricas. Porém, o uso de diferentes teores e tipos de estabilizantes pode alterar o comportamento reológico das resinas. Neste trabalho foi avaliado o efeito de estabilizantes UV, em diferentes proporções na reologia de duas resinas comerciais à base de epoxi e poliéster. Os estabilizantes orgânicos têm diferentes viscosidades. Ambos os estabilizantes reduzem a viscosidade das resinas. Em ensaios tixotrópicos não foi observado variação significativa em função do teor de estabilizante. A tensão limite de escoamento das resinas foi determinada por varredura de deformação. O valor da tensão limite obtida pela curva de fluxo foi avaliada também pelo ensaio dinâmico sob tensão constante. As metodologias comprovaram o efeito de modificador de viscosidade dos estabilizantes

Perception of the level of physical activity of university professors during the isolation of COVID-19 / Percepção do nível de atividade física dos professores universitários durante o isolamento da COVID-19

COVID-19 is characterized by a severe acute respiratory syndrome (SARS), caused by the SARS-CoV-2 coronavirus, which started in China, in Wuhan, spreading throughout the world in an advanced way and has become a global health emergency, affecting 216 countries. The most recent number of confirmed cases of COVID- 19 is> 23.3 million worldwide, including> 806.4 confirmed deaths, as well as having forced> 4 billion people to be confined to their homes. In Brazil, more than 3.5 million people have been infected, with a total of more than 114,250 confirmed deaths today. On January 30, 2020, WHO notified COVID-19 as the sixth international public health emergency. The uncertainty of not knowing when the pandemic will end can affect people's physical health and can lead to other associated symptoms. The objective of the study was to investigate the consequences that social isolation can cause in aspects of physical activity in teachers of all undergraduate courses at the Centro Universitário Escritor Osman da Costa Lins - UNIFACOL, in Vitória de Santo Antão-PE. A cross-sectional study was carried out. Data collection took place through online questionnaires containing sociodemographic information and levels of physical activity. There were 115 participants, of which 57.4% were male and 42.6% female. Regarding the level of physical activity, there was a statistically significant difference between the sexes in moderate-intensity and walking. And during the pandemic, there were more active women and more sedentary men

Chromosomal phylogeny and comparative chromosome painting among Neacomys species (Rodentia, Sigmodontinae) from eastern Amazonia

Abstract: Background: The Neacomys genus is predominantly found in the Amazon region, and belongs to the most diverse tribe of the Sigmodontinae subfamily (Rodentia, Cricetidae, Oryzomyini). The systematics of this genus and questions about its diversity and range have been investigated by morphological, molecular (Cytb and COI sequences) and karyotype analysis (classic cytogenetics and chromosome painting), which have revealed candidate species and new distribution areas. Here we analyzed four species of Neacomys by chromosome painting with Hylaeamys megacephalus (HME) whole-chromosome probes, and compared the results with two previously studied Neacomys species and with other taxa from Oryzomyini and Akodontini tribes that have been hybridized with HME probes. Maximum Parsimony (MP) analyses were performed with the PAUP and T.N.T. software packages, using a non-additive (unordered) multi-state character matrix, based on chromosomal morphology, number and syntenic blocks. We also compared the chromosomal phylogeny obtained in this study with molecular topologies (Cytb and COI) that included eastern Amazonian species of Neacomys, to define the phylogenetic relationships of these taxa. Results: The comparative chromosome painting analysis of the seven karyotypes of the six species of Neacomys shows that their diversity is due to 17 fusion/fission events and one translocation, pericentric inversions in four syntenic blocks, and constitutive heterochromatin (CH) amplification/deletion of six syntenic autosomal blocks plus the X chromosome. The chromosomal phylogeny is consistent with the molecular relationships of species of Neacomys. We describe new karyotypes and expand the distribution area for species from eastern Amazonia and detect complex rearrangements by chromosome painting among the karyotypes. Conclusions: Our phylogeny reflects the molecular relationships of the Akodontini and Oryzomyini taxa and supports the monophyly of Neacomys. This work presents new insights about the chromosomal evolution of this group, and we conclude that the karyotypic divergence is in accord with phylogenetic relationships

EVALUATION OF DRUG PRESCRIPTIONS IN A HOSPITAL EMERGENCY DEPARTMENT

Objective: To evaluate the adherence of medical prescriptions to patient safety recommendations using the Medication Prescription Safety Checklist.Method: This is an observational, cross-sectional study carried out between May and June 2022, with 341 medical prescriptions for medicines in a hospital emergency room in the interior of Bahia - Brazil, whose data were analyzed through descriptive analysis.Results: 80% to 89% of the prescriptions adhered to the safety recommendations; the item with the highest adherence was identification of the date of the prescription, and the lowest adherence was having drugs with similar names identified in upper case or bold. Around 18.63% (n=514) of the drugs prescribed are on the list of potentially dangerous drugs for hospital use.Conclusion: The evaluation of medical prescriptions for medicines highlighted existing barriers in clinical practice, which makes it possible to develop more effective mechanisms to promote patient safety

Karyotypic divergence reveals that diversity in the Oecomys paricola complex (Rodentia, Sigmodontinae) from eastern Amazonia is higher than previously thought.

The genus Oecomys (Rodentia, Sigmodontinae) is distributed from southern Central America to southeastern Brazil in South America. It currently comprises 18 species, but multidisciplinary approaches such as karyotypic, morphological and molecular studies have shown that there is a greater diversity within some lineages than others. In particular, it has been proposed that O. paricola constitutes a species complex with three evolutionary units, which have been called the northern, eastern and western clades. Aiming to clarify the taxonomic status of O. paricola and determine the relevant chromosomal rearrangements, we investigated the karyotypes of samples from eastern Amazonia by chromosomal banding and FISH with Hylaeamys megacephalus (HME) whole-chromosome probes. We detected three cytotypes for O. paricola: A (OPA-A; 2n = 72, FN = 75), B (OPA-B; 2n = 70, FN = 75) and C (OPA-C; 2n = 70, FN = 72). Comparative chromosome painting showed that fusions/fissions, translocations and pericentric inversions or centromeric repositioning were responsible for the karyotypic divergence. We also detected exclusive chromosomal signatures that can be used as phylogenetic markers. Our analysis of karyotypic and distribution information indicates that OPA-A, OPA-B and OPA-C are three distinct species that belong to the eastern clade, with sympatry occurring between two of them, and that the "paricola group" is more diverse than was previously thought

PREVENÇÃO DE CÂNCER DE COLO DO ÚTERO NA ESTRATÉGIA DE SAÚDE DA FAMÍLIA: DESENVOLVIMENTO E APLICAÇÃO DE UMA CARTILHA INSTRUTIVA

O câncer de colo do útero (CCU) é uma doença profilática quando diagnóstica precocemente, no entanto a mesma ainda continua sendo um impacto para a saúde pública no Brasil. Segundo pesquisas, o CCU é considerado como o quarto tipo de câncer que mais afeta a população feminina, ocorrendo principalmente em populações de baixa renda e escassa de informações. Geralmente a doença surge a partir dos 30 anos e estende rapidamente os riscos até a faixa etária acima de 50. Nesse contexto, torna-se necessário à implementação de estratégias efetivas de controle dessa enfermidade, como a utilização de métodos educativos como a cartilha que serve de suporte para profissionais e usuários facilitando a orientação. A cartilha implantada atuará diretamente nas ações de saúde fortalecendo as diretrizes da estratégia saúde da família e da educação em saúde. Trata-se de uma pesquisa tecnológica e descritiva, com abordagem quantitativa, onde será realizada no Serviço Especial de Saúde Pública - SESP, no município de Quixadá/CE, com mulheres com idade maior ou igual a 18 anos assistidas na referida unidade. Portanto, o presente trabalho tem por objetivo permitir a troca de informações imediata, ampliando o conhecimento dos usuários e profissionais, esclarecendo duvidas sobre o exame Papanicolau e dicas como benefícios de suporte ao câncer