Developmental endothelial locus-1 protects from hypertension-induced cardiovascular remodeling via immunomodulation

The causative role of inflammation in hypertension-related cardiovascular diseases is evident and calls for development of specific immunomodulatory therapies. We tested the therapeutic efficacy and mechanisms of action of developmental endothelial locus-1 (DEL-1), an endogenous anti-inflammatory factor, in angiotensin-II (ANGII)- and DOCA (deoxycorticosterone acetate)-salt-induced cardiovascular organ damage and hypertension. By using mice with endothelial overexpression of DEL-1 (EC-Del1) and performing preventive and interventional studies by injecting recombinant DEL-1 in mice, we showed that DEL-1 improved endothelial function and abrogated aortic adventitial fibrosis, medial thickening and loss of elastin. DEL-1 also protected the mice from cardiac concentric hypertrophy, interstitial and perivascular coronary fibrosis and improved left-ventricular function and myocardial coronary perfusion. DEL-1 prevented aortic stiffness and abolished the progression of hypertension. Mechanistically, DEL-1 acted by inhibiting αvβ3-integrin dependent activation of pro-MMP2 in mice and in human isolated aorta. Moreover, DEL-1 stabilized αvβ3-integrin dependent CD25+FoxP3+ Treg numbers and IL-10 levels, which were associated with decreased pro-inflammatory cell recruitment of inflammatory cells and reduced production of pro-inflammatory cytokines in cardiovascular organs. The demonstrated effects and immune-modulating mechanisms of DEL-1 in abrogation of cardiovascular remodeling and progression of hypertension identify DEL-1 as a potential therapeutic factor

Ten golden rules for optimal antibiotic use in hospital settings: the WARNING call to action

Antibiotics are recognized widely for their benefits when used appropriately. However, they are often used inappropriately despite the importance of responsible use within good clinical practice. Effective antibiotic treatment is an essential component of universal healthcare, and it is a global responsibility to ensure appropriate use. Currently, pharmaceutical companies have little incentive to develop new antibiotics due to scientific, regulatory, and financial barriers, further emphasizing the importance of appropriate antibiotic use. To address this issue, the Global Alliance for Infections in Surgery established an international multidisciplinary task force of 295 experts from 115 countries with different backgrounds. The task force developed a position statement called WARNING (Worldwide Antimicrobial Resistance National/International Network Group) aimed at raising awareness of antimicrobial resistance and improving antibiotic prescribing practices worldwide. The statement outlined is 10 axioms, or “golden rules,” for the appropriate use of antibiotics that all healthcare workers should consistently adhere in clinical practice

The perceived association between audit rotation and audit quality: Evidence from the UAE

Purpose: the purpose of this paper is to explore the perceived association between audit rotation (AR) and audit quality (AQ) using respondents from a sample of audit firms operating in a developing economy, the United Arab Emirates (UAE). The paper addresses the following research question: How do UAE auditors perceive the association between various forms of AR and AQ?Design/Methodology: we collected perception data from a sample of UAE auditors using a questionnaire, and applied several non-parametric statistical techniques to analyze the data, and to answer five exploratory research questions on the perceived association between various forms of AR and AQ.Findings: the findings suggest that the UAE auditors in our sample did not perceive the association between individual types of AR and AQ as significantly different, and that AR in general is essential for AQ improvement and enhances trust in the audit process. Similarly, we find more support for the perception that medium audit tenure is associated with a lower impairment effect on auditor independence. Furthermore, we find no significant differences in perception based on gender, but younger/less experienced professionals and professionals in self-employed practices and small audit firms (compared to other demographics) significantly perceived AR enforceability and AT length to be associated with AQ. Our findings help to enrich our understanding of the perceived AR-AQ association in a relatively new context and less researched audit area in a developing economy.Originality/Value: although lively debates on the question of AR and AQ within the accounting, finance, investment professions and in the financial media continue, there has been relatively limited knowledge and a dearth of empirical studies on this question in most developing economies. Being the first attempt in the country – the UAE, this study contributes towards addressing this gap in empirical knowledge by exploring the perceived association between various forms of AR and AQ in a developing economy

Evidence of microplastics accumulation in the gills and gastrointestinal tract of fishes from an estuarine system in Ghana

The contamination of aquatic environments by microplastics (MPs) and their subsequent ingestion by fish continues to be a universal ecological challenge. Although numerous studies have been conducted on the accumulation of MPs by fishes globally, not much work has been done within the major estuaries along the Atlantic Coast. This study explored and characterized microplastics in the gills and gastrointestinal tract in 98 specimens of 10 fish taxa (Sarotherodon melanotheron, Pseudotolithus senegalensis, Gobionellus occidentalis, Ethmalosa fimbriata, Chrysichthys nigrodigitalus, Elops lacerta, Mugil bananesis, Cynoglossus senegalensis, Apsilus fuscus and Galeoides decadactylus) from the Pra Estuary, Ghana. The gastrointestinal contents of the fish were extracted, analysed and characterized using a stereomicroscope fitted with an Attenuated Total Reflectance Fourier Transform Infrared spectroscopy (ATR-FTIR). A total of 529 MP particles were found in the fishes. C. nigrodigitalus recorded the highest MP levels in the gills with an average of 4.83 ± 2.08 items/individual whiles S. melanotheron recorded the highest in the gastrointestinal tract at 9.83 ± 4.63 items/individual. Within the fish, transparent fibrous MPs of size <0.5 mm were the dominate types found. A vertical prevalence of MPs was observed across the feeding and habitat preference of the species suggesting a possible linkage with the ecological niche of fishes. Our findings further demonstrate the need for advance studies on the impacts and level of threat microplastic accumulation pose to the sampled fishes and potential consumers

Obesity and Cardio-Metabolic Risk Factors in an Urban and Rural Population in the Ashanti Region-Ghana: A Comparative Cross-Sectional Study.

There is a surge in chronic diseases in the developing world, driven by a high prevalence of cardio-metabolic risk factors. This study described differences in prevalence of obesity and cardio-metabolic risk factors between urban and rural settlements in the Ashanti Region of Ghana. This comparative cross-sectional study included 672 participants (median age 50 years), of which 312 were from Kumasi (urban) and 360 from Jachie-Pramso (rural). Demographic, anthropometric and other cardio-metabolic risk factors were gathered and venous blood samples were drawn for biochemical assays. Results suggested significant differences in diastolic blood pressure (80.0 mmHg vs 79.5 mmHg; p = 0.0078), and fasting blood sugar (5.0 mmo/l vs 4.5 mmol/l; p < 0.0001) between the two groups. Further differences in anthropometric measures suggested greater adiposity amongst participants in the urban area. Participants in the urban area were more likely than rural participants, to have high total cholesterol and LDL-c (p < 0.0001 respectively). Risk factors including BMI ≥ 25 (p < 0.0001), BMI ≥ 30 (p < 0.0001), high waist circumference (p < 0.0001), high waist-to-height ratio (p < 0.0001) and alcohol consumption (p = 0.0186) were more prevalent amongst participants in the urban area. Markers of adiposity were higher amongst females than males in both areas (p < 0.05). In the urban area, hypertension, diabetes and lifestyle risk factors were more prevalent amongst males than females. Differences in risk factors by urban/rural residence remained significant after adjusting for gender and age. Obesity and cardio-metabolic risk factors are more prevalent amongst urban settlers, highlighting an urgent need to avert the rise of diet and lifestyle-related chronic diseases

Odds Ratios associated with selected cardio-metabolic factors amongst the urban population (reference: rural population).

<p>OR: Odds ratio. Adj: adjusted. Compared using Multivariate logistic regression. p < 0.05 was considered significant.</p><p>Odds Ratios associated with selected cardio-metabolic factors amongst the urban population (reference: rural population).</p

Socio-demographic characteristics of the study population.

<p>Data is presented as median (IQR); Mann-Whitney test or n (%); Chi-square or Fisher’s test. p < 0.05 was considered significant for rural vs urban. n: number, IQR: Interquartile range. GHS: Ghana Cedi.</p><p>Socio-demographic characteristics of the study population.</p

Spearman’s rho correlation coefficients between selected cardio-metabolic variables for rural (Lower Left-Hand Side) and urban (Upper Right-Hand Side).

<p>** Correlation is significant at the 0.01 level (2-tailed).</p><p>* Correlation is significant at the 0.05 level (2-tailed). SBP: Systolic Blood Pressure. DBP: Diastolic blood Pressure. FBS: Fasting Blood Sugar. WC: Waist circumference. WHR: Waist-to-hip ratio. WHtR: Waist-to-height ratio. BF%: percentage Body Fat. TCHL: Total Cholesterol. HDL-c: High Density Lipoprotein cholesterol. LDL-c: Low Density Lipoprotein Cholesterol. TG: triglycerides.</p><p>Spearman’s rho correlation coefficients between selected cardio-metabolic variables for rural (Lower Left-Hand Side) and urban (Upper Right-Hand Side).</p

Cardio-metabolic risk factors amongst study population stratified by type of community.

<p>Data is presented as n (%); compared using Fischer’s test. p < 0.05 was considered significant for rural vs urban. BMI: Body mass index. WC: Waist Circumference. WHR: Waist-to hip Ratio. WHtR: Waist-to height Ratio. BF%: Percentage body fat. HDL-c: High Density Lipoprotein cholesterol. LDL-c: Low Density Lipoprotein Cholesterol.</p><p>Cardio-metabolic risk factors amongst study population stratified by type of community.</p