Detection of newborn infants at risk for congenitat toxoplasmosis in Rio de Janeiro, Brazil

Within the third to fifth day after birth, btood samples for indirect immunefluorescence tests (rF) for toxoplasmosis were taken from l0B2 neonates. B?? (36.50/o) rF.rgG and rF-rgM seronegative cases and 65s (68.b%) rF-rgG seropositive cases reacting from 1:16 to 1:1024 were found. rn 1b such cases e.4%), sera were reactive in both the IgG and IgM classes, 12 cases being IF-IgM : 1:16, and B cases IF-I9M - L:64. These cases were considered. at potential risks for congenital infection þy T. gondii. of these 15 cases, a morphological study of 18 placentas showed data suggestive of prolonged fetal injuries in 11 such cases. All these 13 placentas showed signs of hematogenous inflammatory processes. Four placentas revealed structures with morphological characteristics similar to cysts of Toxo. plasma gondii at the microscopical examination. Of these four neonates where the parasite'was found in the placentas, one infant was underweight and showed discrete microcephaly. Two children had hepatosplenomegaþ, one of them with unilateral retinochoroiditis. The fourth case was clinicalþ normal. In the other 11 cases, selected due to possible risk of congenital infection, one infant was pre. mature, eight were normal, and two showed symptomatolog¡r non-suggestive of congenital toxoplasmosis

Prospective study of pregnants and babies with risk of congenital toxoplasmosis in municipal district of Rio Grande do Sul

This study followed up 2,126 pregnant women cared for at SUS day-care clinics (Public Health Insurance System) of the northwest of the State of Rio Grande do Sul, Brazil. After serological screening we performed a follow up of all pregnant women and their babies. Serologic tests included: IgG, IgM, IgA and IgG avidity levels, mice inoculation and polymerase chain reaction (PCR) also placentas and umbilical materials were tested using immunoperoxidase as well as clinical evaluation. Of all the pregnant women screened, 74.5% were reactive to toxoplasmosis, and 3.6% presented IgM seropositivity. At ophthalmic evaluation ten women had ocular lesions and one infant presented eye lesions and brain calcification. The presence of anti-T.gondii specific IgM throughout the entire pregnancy did not characterize acute phase infection, for this, complementary tests were necessary. The importance is underscored for attendance of the newborn of mothers presenting serology compatible with this infection even in the absence of signs and symptoms of congenital toxoplasmosis.A população estudada foi composta por 2.126 gestantes atendidas em unidades do Sistema Único de Saúde da região noroeste do Estado do Rio Grande do Sul. Após o screening sorológico inicial ocorreu o acompanhamento das gestantes, durante o pré-natal, e de seus bebês. Foram realizadas dosagens de IgG, IgM, IgA, Avidez de IgG, inoculação em camundongos, PCR e coleta de placenta e de cordões umbilicais para realizar a técnica de imuno-histoquímica além de avaliações clínicas. Das gestantes avaliadas, 74,5% eram IgG reagentes e 3,6% IgM reagentes. Nas avaliações oftalmológicas, foi observada lesão em dez gestantes e uma criança apresentou lesões oftalmológicas e calcificações cerebrais. A presença de IgM específico anti-T.gondii, durante toda a gestação não caracterizou a fase aguda recente da infecção, fazendo-se necessária a realização de testes complementares. Ressalta-se a importância do acompanhamento de neonatos de mães com sorologia compatível com a infecção mesmo sem sinais e sintomas sugestivos de toxoplasmose congênita.Fundação Estadual de Produção e Pesquisa em Saúde Secretaria da Saúde do Estado do Rio Grande do Sul Laboratório Central do EstadoUniversidade Federal do Rio Grande do Sul Faculdade de FarmáciaFundação Oswaldo Cruz Instituto Oswaldo Cruz Departamento de ProtozoologiaHospital da Criança Santo AntônioEscola Paulista de MedicinaFundação Oswaldo Cruz Instituto Fernandes Figueira Departamento de Anatomia PatológicaFundação Oswaldo Cruz Escola Nacional de Saúde Pública Departamento de Ciências BiológicasUNIFESP, EPMSciEL

Enquête écoépidémiologique de Toxoplasma gondii chez différentes espèces animales domestiques et sauvages de l'Etat de Rio de Janeiro au Brésil.

International audienc

Toxoplasma gondii: epidemiological study of pigs from southwestern Paraná, Brazil

Submitted by Sandra Infurna ([email protected]) on 2020-02-11T15:29:39Z No. of bitstreams: 1 RegianeVicente_TatianaCosta_etal_IOC_2008.pdf: 109483 bytes, checksum: bccb1603a023c2f544c0fa572b242bec (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2020-02-11T15:38:37Z (GMT) No. of bitstreams: 1 RegianeVicente_TatianaCosta_etal_IOC_2008.pdf: 109483 bytes, checksum: bccb1603a023c2f544c0fa572b242bec (MD5)Made available in DSpace on 2020-02-11T15:38:37Z (GMT). No. of bitstreams: 1 RegianeVicente_TatianaCosta_etal_IOC_2008.pdf: 109483 bytes, checksum: bccb1603a023c2f544c0fa572b242bec (MD5) Previous issue date: 2008Universidade Federal Fluminense. Faculdade de Veterinária. Pós-Graduação em Higiene Veterinária e Processamento Tecnológico de Produtos de Origem Animal. Niterói, RJ, Brasil.Ministério da Agricultura, Pecuária e Abastecimento. Serviço de Inspeção Federal. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Veterinária. Departamento de Saúde Coletiva Veterinária e Saúde Pública. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil.This study evaluated the seroprevalence of toxoplasmosis in pigs bred and slaughtered for human consumption. The frequency of the antibody anti-Toxoplasma gondii was investigated in 408 animals from distinct piggeries of 25 cities in the southwestern region of the state of Paraná, Brazil. Serum samples were examined for IgG antibodies specific for T. gondii through the indirect fluorescent antibody test (IFAT). A frequency of 25.5% of serum samples tested positive for dilutions equal to or greater than 1:64. statistically significant were not observed in relation to sex and age of the animals. The highest antibody level observed was 4,096 (3.0%) and the most frequent was 64 (74%), suggesting that a chronic infection occurred in the population. The high prevalence of the infection found in the swine herds of this region suggests that these herds can be a source of infection for individuals who typically consume this type of raw or badly cooked meat

Ocorrência e fatores de risco da infecção por Toxoplasma gondii em suínos criados e abatidos na região do Triângulo Mineiro, Minas Gerais, Brasil

Submitted by Sandra Infurna ([email protected]) on 2018-02-06T12:01:41Z No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-02-06T12:14:56Z (GMT) No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5)Made available in DSpace on 2018-02-06T12:14:56Z (GMT). No. of bitstreams: 1 mariaregina_amendoeira_etal_IOC_2017.pdf: 917033 bytes, checksum: 74384c0b1588811f16d472eb6cf3a6a7 (MD5) Previous issue date: 2017Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozoonoses. Rio de Janeiro, RJ. Brasil.Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Uberlândia, MG, Brasil.Sem afiliaçãoUniversidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Parasitologia. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozoonoses. Rio de Janeiro, RJ. Brasil.The Triângulo Mineiro region from Minas Gerais state, is an important meat-exporting region of Brazil and data about Toxoplasma gondii infection in pigs raised and slaughtered in this area are scarce. Therefore, the aim of this study was to evaluate the occurrence of T. gondii in swine and establish the risk factors associated with the infection. Samples were collected from 600 pigs raised under intensive system in farms located at three different counties (Carmo do Paranaíba, Patrocínio and Perdizes). The samples were submitted to indirect hemagglutination antibody test with dilution of 1:32 and to indirect immunofluorescence antibody test with a cutoff of 1:64. The occurrence of positive pig was 3.3% (n=20) and 51.8% (n=311) respectively. A significant difference was observed between toxoplasmatic infection and factors such as lineage, animal origin, size of the farm, collective raising with others species, presence of rodents and type of water offered (p≤0.05). There was no difference between gender and the farm goals. The results demonstrated an occurrence of anti-T. gondii antibodies higher than expected for intensive pig raising system on the studied area, which could indicate a possible sanitary management problem on the studied proprieties. Improvements on the raising techniques are necessary to reduce T. gondii infection sources

Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

ABSTRACT: The Triângulo Mineiro region from Minas Gerais state, is an important meat-exporting region of Brazil and data about Toxoplasma gondii infection in pigs raised and slaughtered in this area are scarce. Therefore, the aim of this study was to evaluate the occurrence of T. gondii in swine and establish the risk factors associated with the infection. Samples were collected from 600 pigs raised under intensive system in farms located at three different counties (Carmo do Paranaíba, Patrocínio and Perdizes). The samples were submitted to indirect hemagglutination antibody test with dilution of 1:32 and to indirect immunofluorescence antibody test with a cutoff of 1:64. The occurrence of positive pig was 3.3% (n=20) and 51.8% (n=311) respectively. A significant difference was observed between toxoplasmatic infection and factors such as lineage, animal origin, size of the farm, collective raising with others species, presence of rodents and type of water offered (p≤0.05). There was no difference between gender and the farm goals. The results demonstrated an occurrence of anti-T.gondii antibodies higher than expected for intensive pig raising system on the studied area, which could indicate a possible sanitary management problem on the studied proprieties. Improvements on the raising techniques are necessary to reduce T. gondii infection sources

Soroepidemiologia da toxoplasmose em caprinos e ovinos de três municípios do estado do Rio de Janeiro

A toxoplasmose é uma zoonose de ampla distribuição mundial, causada pelo Toxoplasma gondii. O estudo da prevalência desta infecção em animais produtores de carne e leite é de interesse à saúde pública, devido ao fato desses produtos oriundos de animais infectados serem importantes vias de transmissão para o homem, quando consumidos in natura. Além disso, há o aspecto econômico, uma vez que pode causar aborto, retardo no crescimento e animais debilitados, levando prejuízos ao pecuarista. Este trabalho objetivou estimar a soroprevalência da infecção por T. gondii, por meio da reação de imunofluorescência indireta (RIFI) em caprinos e ovinos de três municípios do estado do Rio de Janeiro, provenientes de 10 propriedades. A prevalência de anticorpos IgG anti-T.gondii foi de 29,12% (60/206) nos caprinos e de 38,05% (137/360) nos ovinos, sendo observada nessa última espécie associação (p<0,05) entre sexo (fêmeas), idade adulta, sistema de criação extensivo, dieta de pastagem e água de beber de açude com a soropositividade. Os títulos variaram de 64 a 256, podendo ser sugestivos de infecção crônica. Melhorias nas técnicas de criação podem reduzir as fontes de infecção por T. gondii nos rebanhos

Seroepidemiology of toxoplasmosis in goats and sheep from three counties of Rio de Janeiro state, Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-03-27T11:47:05Z No. of bitstreams: 1 magyda_duharoug_etal_IOC_2011.pdf: 167561 bytes, checksum: 27921249420884bbe655f43b68e22dbf (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-03-27T11:54:46Z (GMT) No. of bitstreams: 1 magyda_duharoug_etal_IOC_2011.pdf: 167561 bytes, checksum: 27921249420884bbe655f43b68e22dbf (MD5)Made available in DSpace on 2018-03-27T11:54:47Z (GMT). No. of bitstreams: 1 magyda_duharoug_etal_IOC_2011.pdf: 167561 bytes, checksum: 27921249420884bbe655f43b68e22dbf (MD5) Previous issue date: 2011Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Zoonoses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Zoonoses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório deToxoplasmose. Rio de Janeiro, RJ. Brasil.Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii. Epidemiological surveys of T. gondii infection among livestock have great economical importance since this infection may cause abortion, growth retardation and neonatal mortality, with significant losses to breeders. In regard of public health, human infection can be acquired by ingestion of meat or milk in natura from infected livestock. The aim of this study was to assess the toxoplasmosis seroprevalence by indirect immunofluorescence antibody test (IFAT) in goats and sheep, in three counties of Rio de Janeiro state, from 10 different farms. The seroprevalences of anti-T.gondii IgG antibodies were 29.12% (60/206) in goats and 38.05% (137/360) in sheep, with titers ranging from 64 to 256, suggesting chronic infection. Association of each of the following epidemiological factors: female gender, adult age, extensive management system, grazing pasture and drinking lake water with seropositivity was observed only in sheep (p≤0.05). Improvement in breeding conditions may reduce the sources of infection in herds