Antibody Conjugation and Formulation

In an era where ultra-high antibody concentrations, high viscosities, low volumes, auto-injectors, and long storage requirements are already complex problems with the current unconjugated monoclonal antibodies on the market the formulation demands for antibody-drug conjugates (ADCs) are significant. Antibodies have historically been administered at relatively low concentrations through intravenous (IV) infusion due to their large size and the inability to formulate for oral delivery. Due to the high demands associated with IV infusion and the development of novel antibody targets and unique antibody conjugates more accessible routes of administration such as intramuscular (IM), and subcutaneous (SC) are being explored. This review will summarize various site-specific and non-site-specific antibody conjugation techniques in the context of antibody-drug conjugates (ADCs) and the demands of formulation for high concentration clinical implementation

Etyma for 'chicken', 'duck', and 'goose' among language phyla in China and Southeast Asia

This paper considers the history of words for domesticated poultry, including ‘chicken’, ‘goose’, and ‘duck’, in China and mainland Southeast Asia to try to relate associated domestication events with specific language groups. Linguistic, archaeological and historical evidence supports Sinitic as one linguistic source, but in other cases, Tai and Austroasiatic form additional centers of lexical forms which were borrowed by neighboring phyla. It is hypothesized that these geographic regions of etyma for domesticated birds may represent instances of bird domestication, or possibly advances in bird husbandry, by speech communities in the region in the Neolithic Era, followed by spread of both words and cultural practices

Orion Revisited - I. The massive cluster in front of the Orion Nebula Cluster

The aim of this work is to characterize the stellar population between Earth and the Orion A molecular cloud where the well known star formation benchmark Orion Nebula Cluster (ONC) is embedded. We use the denser regions the Orion A cloud to block optical background light, effectively isolating the stellar population in front of it. We then use a multi-wavelength observational approach to characterize the cloud's foreground stellar population. We find that there is a rich stellar population in front of the Orion A cloud, from B-stars to M-stars, with a distinct 1) spatial distribution, 2) luminosity function, and 3) velocity dispersion from the reddened population inside the Orion A cloud. The spatial distribution of this population peaks strongly around NGC 1980 (iota Ori) and is, in all likelihood, the extended stellar content of this poorly studied cluster. We infer an age of ~4-5 Myr for NGC 1980 and estimate a cluster population of the order of 2000 stars, which makes it one of the most massive clusters in the entire Orion complex. What is currently taken in the literature as the ONC is then a mix of several intrinsically different populations, namely: 1) the youngest population, including the Trapezium cluster and ongoing star formation in the dense gas inside the nebula, 2) the foreground population, dominated by the NGC 1980 cluster, and 3) the poorly constrained population of foreground and background Galactic field stars. Our results support a scenario where the ONC and L1641N are not directly associated with NGC 1980, i.e., they are not the same population emerging from its parental cloud, but are instead distinct overlapping populations. This result calls for a revision of most of the observables in the benchmark ONC region (e.g., ages, age spread, cluster size, mass function, disk frequency, etc.). (abridged)Comment: Version 2 includes comments and clarifications from John Tobin, Nicola Da Rio, and Lynne Hillenbrand (minor clarifying changes were made to Figures 1, 8, and 10). A&A accepted (15 pages, 10 figures). Higher resolution figures available upon reques

On the trace anomaly and the energy-momentum conservation of quantum fields at D=2 in classical curved backgrounds

We study the conformal symmetry and the energy-momentum conservation of scalar field interacting with a curved background at D=2. We avoid to incorporate the metric determinant into the measure of the scalar field to explain the conformal anomaly and the consequent energy-momentum conservation. Contrarily, we split the scalar field in two other fields, in such a way that just one of them can be quantized. We show that the same usual geometric quantities of the anomaly are obtained, which are accompanied by terms containing the new field of the theory.Comment: 5 pages, no figure

Hipparcos distances of Ophiuchus and Lupus cloud complexes

We combine extinction maps from the Two Micron All Sky Survey (2MASS) with Hipparcos and Tycho parallaxes to obtain reliable and high-precision estimates of the distance to the Ophiuchus and Lupus dark complexes. Our analysis, based on a rigorous maximum-likelihood approach, shows that the rho-Ophiuchi cloud is located at (119 +/- 6) pc and the Lupus complex is located at (155 +/- 8) pc; in addition, we are able to put constraints on the thickness of the clouds and on their orientation on the sky (both these effects are not included in the error estimate quoted above). For Ophiuchus, we find some evidence that the streamers are closer to us than the core. The method applied in this paper is currently limited to nearby molecular clouds, but it will find many natural applications in the GAIA-era, when it will be possible to pin down the distance and three-dimensional structure of virtually every molecular cloud in the Galaxy.Comment: A&A in press - Corrected typo (Lupus distance) in the electronic abstrac

Fibers in the NGC1333 proto-cluster

Are the initial conditions for clustered star formation the same as for non-clustered star formation? To investigate the initial gas properties in young proto-clusters we carried out a comprehensive and high-sensitivity study of the internal structure, density, temperature, and kinematics of the dense gas content of the NGC1333 region in Perseus, one of the nearest and best studied embedded clusters. The analysis of the gas velocities in the Position-Position-Velocity space reveals an intricate underlying gas organization both in space and velocity. We identified a total of 14 velocity-coherent, (tran-)sonic structures within NGC1333, with similar physical and kinematic properties than those quiescent, star-forming (aka fertile) fibers previously identified in low-mass star-forming clouds. These fibers are arranged in a complex spatial network, build-up the observed total column density, and contain the dense cores and protostars in this cloud. Our results demonstrate that the presence of fibers is not restricted to low-mass clouds but can be extended to regions of increasing mass and complexity. We propose that the observational dichotomy between clustered and non-clustered star-forming regions might be naturally explained by the distinct spatial density of fertile fibers in these environments.Comment: 25 pages, 17 figures; Accepted for publication in A&

Uncovering the Beast: Discovery of Embedded Massive Stellar Clusters in W49A

We present subarcsecond J, H, and Ks images (FWHM ~ 0.5") of an unbiased 5'x 5' (16pc x 16pc) survey of the densest region of the W49 giant molecular cloud. The observations reveal 4 massive stellar clusters (with stars as massive as \~120 Msun), the larger (Cluster 1) about 3 pc East of the well known Welch ring of ultra-compact Hii regions. Cluster 1 is a) extincted by at least Av > 20 mag of foreground (unrelated and local) extinction, b) has more than 30 mag of internal inhomogeneous visual extinction implying that it is still deeply buried in its parental molecular cloud, and c) is powering a 6 pc diameter giant Hii region seen both at the NIR and radio continuum. We also identify the exciting sources of several UCHii regions. The census of massive stars in W49A agrees or is slightly overabundant when compared with the number of Lyman continuum photons derived from radio observations. We argue that although the formation of the Welch ring could have been triggered by Cluster 1, the entire W49A starburst region seems to have been multi-seeded instead of resulting from a coherent trigger.Comment: ApJ Letters accepted. All figures provided as nice resolution jpeg/gif files. Get full-res version at http://www.eso.org/~jalves/W49A.pd

Gravitational collapse of the OMC-1 region

We have investigated the global dynamical state of the Integral Shaped Filament in the Orion A cloud using new N$_2$H$^+$ (1-0) large-scale, IRAM30m observations. Our analysis of its internal gas dynamics reveals the presence of accelerated motions towards the Orion Nebula Cluster, showing a characteristic blue-shifted profile centred at the position of the OMC-1 South region. The properties of these observed gas motions (profile, extension, and magnitude) are consistent with the expected accelerations for the gravitational collapse of the OMC-1 region and explain both the physical and kinematic structure of this cloud.Comment: 5 pages, 2 figures; Accepted by A&