Nuclear response functions for the N-N*(1440) transition

Parity-conserving and -violating response functions are computed for the inclusive electroexcitation of the N*(1440)(Roper) resonance in nuclear matter modeled as a relativistic Fermi gas. Using various empirical parameterizations and theoretical models of the N-N*(1440) transition form factors, the sensitivity of the response functions to details of the structure of the Roper resonance is investigated. The possibility of disentangling this resonance from the contribution of Delta electroproduction in nuclei is addressed. Finally, the contributions of the Roper resonance to the longitudinal scaling function and to the Coulomb sum rule are also explored.Comment: 25 pages, 10 figures. Minor changes in the Introduction. Accepted in NP

The two pion decay of the Roper resonance

We evaluate the two pion decay of the Roper resonance in a model where explicit re-scattering of the two final pions is accounted for by the use of unitarized chiral perturbation theory. Our model does not include an explicit $\epsilon$ or $\sigma$ scalar-isoscalar meson decay mode, instead it generates it dynamically by means of the pion re-scattering. The two ways, explicit or dynamically generated, of introducing this decay channel have very different amplitudes. Nevertheless, through interference with the other terms of the model we are able to reproduce the same phenomenology as models with explicit consideration of the $\epsilon$ meson.Comment: 17 latex pages, 11 eps figures. A few misprints corrected. A few new references. Version accepted for publication in Phys. Rev.

The seminal plasma proteins Peptidyl arginine deaminase 2, rRNA adenine N (6)-methyltransferase and KIAA0825 are linked to better motility post thaw in stallions

Seminal plasma plays an important role in sperm physiology. Seminal plasma proteins vehiculated in microvesicles, carry RNAs and proteins with a potential role in early embryo development. Additionally, proteins present in seminal plasma participate in redox regulation and energy metabolism. In view of these facts, we hypothesized that differences in protein composition of the seminal plasma among stallions may help to explain differences in freeze-ability seen among them. Three independent ejaculates from 10 different stallions of varying breeds were frozen using standard protocols in our laboratory. Aliquots of the ejaculate were separated and stored at −80 °C until further proteomic analysis. Semen analysis was performed using computer assisted sperm analysis and flow cytometry. Significant differences in proteome composition of seminal plasma were observed in the group of stallions showing better motility post thaw. 3116 proteins were identified, and of these, 34 were differentially expressed in stallions with better motility post thaw, 4 of them were also differentially expressed in stallions with different percentages of linearly motile sperm post thaw and 1 protein, Midasin, was expressed in stallions showing high circular velocity post thaw. Seminal plasma proteins may play a major role in sperm functionality; being vehiculated through extracellular vesicles and participating in sperm physiology. Bioinformatic analysis identifies discriminant proteins able to predict the outcome of cryopreservation, identifying potential new biomarkers to assess ejaculate quality

Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility

Even in stallions with sperm quality within normal reference ranges at ejaculation, subtle differences in sperm quality exist that in many cases lead to reduced time frames for conservation of the ejaculate and/or reduced fertility. The spermatozoon is a cell highly suitable for proteomics studies, and the use of this technique is allowing rapid advances in the understanding of sperm biology. The aim of the present study was to investigate differences among stallions of variable sperm quality (based on motility and sperm velocities), although all horses had sperm characteristics within normal ranges. The proteome was studied using UHPLC/MS/MS and posterior bioinformatic and enrichment analysis; data are available via ProteomeXchange with identifier PXD025807. Sperm motility, linear motility and circular, straight line and average velocities (VCL, VSL, VAP) were measured using computer assisted sperm analysis (CASA). In stallions showing better percentages of motility, circular and average velocity predominated mitochondrial proteins with roles in the Citric acid cycle, pyruvate metabolism and oxidative phosphorylation. Interestingly, in stallions with better percentages of total motility, sperm proteins were also enriched in proteins within the gene ontology (G0) terms, single fertilization (G0: 0007338), fertilization (G0: 0009566), and zona pellucida receptor complex (GO:0002199). The enrichment of this proteins in samples with better percentages of total motility may offer a molecular explanation for the link between this parameter and fertility. Significance: Proteomic analysis identified a high degree of specificity of stallion sperm proteins with discriminant power for motility, linear motility, and sperm velocities (VCL, VAP and VSL). These findings may represent an interesting outcome in relation to the molecular biology regulating the movement of the spermatozoa, and the biological meaning of the measurements that computer assisted sperm analysis (CASA) provide. Of a total of 903 proteins identified in stallion spermatozoa, 24 were related to the percentage of total motility in the sample; interestingly, gene ontology (G0) analysis revealed that these proteins were enriched in terms like single fertilization and fertilization, providing a molecular link between motility and fertility. Field studies indicate that the percentage of total motility is the CASA derived parameter with the best correlation with fertility in stallions

Effects of deficit irrigation with saline water on yield and grape composition of Vitis vinifera L. cv. Monastrell

[EN] Warm and semi-arid climates are characterized by rainfall scarcity, resulting in the frequent use of low-quality water for irrigation. This work was undertaken to study the effects of water stress and saline irrigation on yield and grape composition of Monastrell grapevines grafted onto 1103P rootstock. The experiment was carried out during three consecutive seasons in a commercial vineyard located in Jumilla (SE Spain) with a loamy-sandy soil. Rainfed vines were compared with five watering regimes including a Control, irrigated with standard water, and four treatments that combined two different schedules for irrigation initiation (pre- and post-veraison) with saline water obtained by adding two types of salts (sulphates and chlorides). Vines from treatments with more severe water stress (i.e., rainfed) showed lower yields and vegetative growth. Moreover, the Rainfed treatment clearly modified grape composition when compared with the Control treatment by increasing berry phenolic content. The application of saline water slightly affected vine performance and grape composition regardless of the type of salts added to the irrigation water. Indeed, the watering regime had a greater effect on yield, vegetative growth and grape composition than the use of different saline waters. Our results suggest that, in the mid-term (3 years), and with a vineyard soil with good drainage, the use of saline waters is not detrimental to vine performance, but does not improve grape composition. Further research is required to assess the long-term effects of saline water application, particularly in view of the important accumulation of chlorides and sodium in leaf tissues observed in vines watered with salty water at the last season of this experiment.Open Access funding provided thanks to the CRUECSIC agreement with Springer Nature. This work was supported by the Spanish Ministry of Economy and Competitiveness with FEDER co-financing [grant numbers AGL-2014-54201-C4-4-R and AGL2017-83738-C3-3-R].Martínez-Moreno, A.; Pérez-Álvarez, E.; Intrigliolo, D.; Mirás-Avalos, J.; López-Urrea, R.; Gil-Muñoz, R.; Lizama Abad, V.... (2023). Effects of deficit irrigation with saline water on yield and grape composition of Vitis vinifera L. cv. Monastrell. Irrigation Science. 41(4):469-485. https://doi.org/10.1007/s00271-022-00795-x469485414Allen RG, Pereira LS, Raes D, Smith M (1998) Crop evapotranspiration. Guidelines for computing crop water requirements. FAO irrigation and drainage, paper 56. FAO, Rome 300(9):D05109Amerine MA, Winkler AJ (1944) Composition and quality of musts and wines of California grapes. Hilgardia. 15:493–675Blouin J (1992) Tecniques d´analyses des moûtes et des vins. Dujardin-Salleron, Paris, FranceBuesa I, Pérez D, Castel J, Intrigliolo DS, Castel JR (2017) Effect of deficit irrigation on vine performance and grape composition of Vitis vinifera L. cv. Muscat of Alexandria. Aust J Grape Wine Res. 23:251–259Castellarin SD, Pfeiffer A, Sivilotti P, Degan M, Peterlunger E, Di Gaspero G (2007) Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant Cell Environ. 30:1381–1399Chalmers YM, Downey MO, Krstic MP, Loveys BR, Dry PR (2010) Influence of sustained deficit Irrigation on colour parameters of Cabernet Sauvignon and Shiraz microscale wine fermentations. Aust J Grape Wine Res. 16:298–316Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot. 103:551–560Choné X, Van Leeuwen C, Dubourdieu D, Gaudillère JP (2001) Stem water potential is a sensitive indicator of grapevine water status. Ann Bot. 87:477–483Cramer GR, Ergui A, Grimplet J, Tillett RL, Tattersall EAR, Bohlman MC (2007) Water and salinity stress in grapevines: early and late changes in transcript and metabolite profiles. Funct Integr Genomics. 7:111–134Dag A, Ben-Gal A, Goldberger S, Yermiyahu U, Zipori I, David I, Netzer Y, Kerem Z (2015) Sodium and chloride distribution in grapevines as a function of rootstock and irrigation water salinity. Am J Enol Vitic. 66:80–84Degaris KA, Walker RR, Loveys BR, Tyerman SD (2016) Comparative effects of deficit and partial root-zone drying irrigation techniques using moderately saline water on ion partitioning in Shiraz and Grenache grapevines. Aust J Grape Wine Res. 22:296–306Deluc LG, Quilici DR, Decendit A, Grimplet J, Wheatley MD, Schlauch KA (2009) Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of Cabernet Sauvignon and Chardonnay. BMC Genomics. 10:212–238Downey MO, Dokoozlian NK, Krstic MP (2006) Cultural practice and environmental impacts on the flavonoid composition of grapes and wine: a review of recent research. Am J Enol Vitic. 57:257–268Downton WJS (1977) Photosynthesis in salt-stressed grapevines. Aust J Plant Physiol 4(2):183–192Ezzhaouani A, Valancogne C, Pieri P, Amalak T, Gaudillère JP (2007) Water economy by Italia grapevines under different irrigation treatments in a Mediterranean climate. J Int Sci Vigne et du Vin. 41:131–139Fernández JE, Alcon F, Diaz-Espejo A, Hernandez-Santana V, Cuevas MV (2020) Water use indicators and economic analysis for on-farm irrigation decision: a casestudy of a super high density olive tree orchard. Agric Water Manag. 237:106074Gambetta GA, Carlos Herrera J, Dayer S, Feng Q, Hochberg U, Castellarin SD (2020) The physiology of drought stress in grapevine: towards an integrative definition of drought tolerance. J Exp Bot. 71:4658–4676Glories Y (1984) La couleur des vins rouges. 1re. Partie: les equilibres des anthocyanes et des tanins. J Int Sci Vigne et du Vin. 18:195–217Hawker JS, Walker RR (1978) The effect of sodium chloride on the growth and fruiting of Cabernet Sauvignon vines. Am J Enol Vitic. 29:172–176Intrigliolo DS, Castel JR (2011) Interactive effects of deficit irrigation and shoot and cluster thinning on grapevine cv. Tempranillo. Water relations, vine performance and berry and wine composition. Irrig Sci 29(6):443–454Intrigliolo DS, Lizama V, García-Esparza MJ, Abrisqueta I, Álvarez I (2016) Effects of post-versions irrigation regime on cabernet sauvignon Grapevines in Valencia, Spain: yield and grape composition. Agric Water Manag. 170:110–119IPCC (International Panel on Climate Change). (2019) Climate Change 2019: Impacts, Adaptation, and Vulnerability. http://ipcc‐wg2.gov/AR5/report/final‐drafts. Accessed 11 Sept 2021Junquera P, Lissarrague JR, Jimenez L, Linares R, Baeza P (2012) Long-term effects of different irrigation strategies on yield components, vine vigour, and grape composition in cv. Cabernet-Sauvignon (Vitis vinifera L.). Irrig Sci. 30:351–361Keller M (2015) The science of grapevines, anatomy and physiology. Second edition. Ed. Elsevier, San Diego, CAKennedy JA, Matthews MA, Waterhouse AL (2002) Effect of maturity and vine water status on grape skin and wine flavonoids. Am J Enol Vitic. 53:268–274Kliewer WM, Dokoozlian NK (2005) Leaf area/crop weight ratios of grapevines: influence on fruit composition and wine quality. Am J Enol Vitic. 56(2):170–181López-Urrea R, Montoro A, Mañas F, López-Fuster P, Fereres E (2012) Evapotranspiration and crop coefficients from lysimeter measurements of mature Tempranillo wine grapes. Agric Water Manag. 112:13–20Maas EV, Hoffman GJ (1977) Crop salt tolerance-current assessment. J Irrig Drain Div 103:115–134Martínez-Moreno A, Pérez-Álvarez EP, López-Urrea R, Paladines-Quezada DF, Moreno-Olivares JD, Intrigliolo DS, Gil-Muñoz R (2021) Effects of deficit irrigation with saline water on wine color and polyphenoliccomposition of Vitis vinifera L. cv. Monastrell. Sci Hortic 283:110085Martínez-Moreno A, Pérez-Álvarez E, López-Urrea R, Intrigliolo D, González-Centeno MR, Teissedre P-L, Gil-Muñoz R (2022) Is deficit irrigation with saline waters a viable alternative for winegrowers in semiarid areas? OENO One 56(1):101–116Matthews MA, Anderson MM (1989) Reproductive development in grape (Vitis vinifera L.): responses to seasonal water deficits. Am J Enol Vitic. 40:52–60Medrano H, Tomás M, Martorell S, Escalona JM, Pou A, Fuentes S, Flexas J, Bota J (2015) Improving water use efficiency of vineyards in semi-arid regions a review. Agron Sustain Dev 35:499–517Mirás-Avalos JM, Intrigliolo DS (2017) Grape composition under abiotic constrains: water stress and salinity. Front Plant Sci. 8:851Munitz S, Netzer Y, Schwartz A (2018) Sustained and regulated deficit irrigation of field-grown Merlot grapevines. Aust J Grape Wine Res. 23:87–94Myers BJ (1988) Water stress integral: a link between short-term stress and long-term growth. Tree Physiol. 4:315–323Nauriyal JP, Gupta OP (1967) Studies on salt tolerance of grape. Effect of total salt concentration. J Wine Res. 4:197–205Netzer Y, Shenker M, Schwartz A (2014) Effects of irrigation using treated wastewater on table grape vineyards: dynamics of sodium accumulation in soil and plant. Irrig Sci. 32:283–294Ojeda H, Andary C, Kraeva E, Carbonnea A, Deloire A (2002) Influence of preand postveraison water deficit on synthesis and concentration of skin phenolic compounds during berry growth of Vitis vinifera cv. Shiraz. Am J Enol Vitic. 53:261–267Ortega JF, De Juan JA, Tarjuelo JM (2005) Improving water management: the irrigation advisory service of Castilla-La Mancha (Spain). Agric Water Manag. 77:37–58Pellegrino A, Lebon E, Simonneau T, Wery J (2005) Towards a simple indicator of water stress in grapevine (Vitis vinifera L.) based on the differential sensitivities of vegetative growth components. Aust J Grape Wine Res. 11:306–315Pereira LS, Paredes P, Jovanovic N (2020) Soil water balance models for determining crop water and irrigation requirements and irrigation scheduling focusing on the FAO56 method and the dual Kc approach. Agric Water Manag. 241:106357Pérez-Álvarez EP, Intrigliolo DS, Vivaldi GA, García-Esparza MJ, Lizama V, Álvarez I (2021) Effects of the irrigation regimes on grapevine cv. Bobal in a Mediterranean climate: I. Water relations, vine performance and grape composition. Agric Water Manag 248:106772Prior LD, Grieve AM, Slavich PG, Cullis BR (1992) Sodium chloride and soil texture interactions in irrigated field grown sultana grapevines. II. Plant mineral content, growth and physiology. Aust J Agric Res. 43:1067–1083Ramos MC, Pérez-Álvarez EP, Peregrina F, Martínez de Toda F (2020) Relationships between grape composition of Tempranillo variety and available soil water and water stress under different weather conditions. Sci Hortic. 262:109063Riquelme F, Martínez-Cutillas A (2018) El libro de la Monastrell. Cofradía del Vino Reino de la Monastrell. Consejería de Agua, Agricultura, Ganadería y Pesca. Murcia. ISBN: 978-84-09-06249-2.Roby G, Harbertson JF, Adams DA, Matthews MA (2004) Berry size and vine water deficits as factors in winegrape composition: anthocyanins and tannins. Aust J Grape Wine Res. 10:100–107Romero P, Fernández-Fernández JI, Martínez-Cutillas A (2010) Physiological thresholds for efficient regulated deficit-irrigation management inwinegrapes grown under semiarid conditions. Am J Enol Vitic. 61:300–312Romero P, Gil-Muñoz R, del Amor FM, Valdés E, Fernández JI, Martinez-Cutillas A (2013) Regulated deficit irrigation based upon optimum water status improves phenolic composition in monastrell grapes and wines. Agric Water Manag. 121:85–101Romero P, García-García J, Fernández-Fernández JI, Gil Muñoz R, del Amor F, Martínez-Cutillas A (2016) Improving berry and wine quality attributes and vineyard economic efficiency by long-term deficit irrigation practices under semiarid conditions. Sci Hortic. 203:69–85Romero P, Botía P, Maria Navarro J (2018) Selecting rootstocks to improve vine performance and vineyard sustainability in deficit irrigated Monastrell grapevines under semiarid conditions. Agric Water Manag. 209:73–93Santesteban LG, Miranda C, Royo JB (2011) Regulated deficit irrigation effects on growth, yield, grape quality and individual anthocyanin composition in Vitis vinifera L. cv. “Tempranillo.” Agric Water Manag. 98:1171–1179Sarneckis CJ, Dambergs RG, Jones P, Mercurio M, Herderich MJ, Smith PA (2006) Quantification of condensed tannins by precipitation with methylcellulose: development and validation of an optimised tool for grape and wine analysis. Aust J Grape Wine Res. 12:39–49Scacco A, Verzera A, Carmela M, Lanza A, Tripodi G, Dima G (2010) Influence of soil salinity on sensory characteristics and volatile aroma compounds of Nero d`Avola Wine. Am J Enol Vitic. 65:498–505Suarez DL, Celis N, Anderson RG, Sandhu D (2019) Grape rootstock response to salinity, water and combined salinity and water stresses. Agron. 9:321Van Leeuwen C, Tregoat O, Choné X, Bois B, Pernet D, Gaudillère JP (2009) Vine water status is a key factor in grape ripening and vintage quality for red Bordeaux wine. How can it be assessed for vineyard management purposes. J Int Sci Vigne du Vin. 43:121–134Vicente-Serrano SM, Lopez-Moreno JI, Beguería S, Lorenzo-Lacruz J, Sanchez-Lorenzo A, García-Ruiz JM, Azorin-Molina C, Morán-Tejeda E, Revuelto J, Trigo R, Coelho F, Espejo F (2014) Evidence of increasing drought severity caused by temperature rise in Southern Europe. Environ Res Lett 9:044001Walker RR, Scott NS, Kriedemann PE (1981) An analysis of photosynthetic response to salt treatment in Vitis vinifera. Aust J Plant Physiol. 8:359–374Walker R, Blackmore D, Clingeleffer P, Iacono F (1997) Effect of salinity and ramsey rootstock on ion concentrations and carbon dioxide assimilation in leaves of drip-irrigated, field-grown grapevines (Vitis vinifera L. cv. Sultana). Aust J Grape Wine Res 3(2):66–74Walker RR, Blackmore DH, Clingeleffer PR, Correll RL (2002) Rootstock effect on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana). Yield and vigour inter-relationships. Aust J Grape Wine Res. 8:3–14Walker RR, Blackmore DH, Clingeleffer PR (2010) Impact of rootstock on yield and ion concentrations in petioles, juice and wine of Shiraz and Chardonnay in different viticultural environments with different irrigation water salinity. Aust J Grape Wine Res 16:243–257Zhang X, Walker RR, Stevens RM, Prior L (2002) Yield salinity relationships of grapevine (Vitis vinifera L.) on own roots and a range of rootstocks. Aust J Grape Wine Res. 8:150–156Zhu JK (2007) Plant Salt, Stress. John Wiley and Sons, Ltd., Nueva Jersey. EEUUZufferey V, Spring JL, Verdenal T, Dienes A, Belcher S, Lorenzini F, Koestel C, Rösti J, Gindro K, Spangenberg J, Viret O (2017) The influence of water stress on plant hydraulics, gas exchange, berry composition and quality of pinot noir wines in Switzerland. OENO One. 51:17–2

On the nature of Delta Scuti star HD 115520

Observing Delta Scuti stars is most important as their multi-frequency spectrum of radial pulsations provide strong constraints on the physics of the stars interior; so any new detection and observation of these stars is a valuable contribution to asteroseismology. While performing uvby-beta photoelectric photometry of some RR Lyrae stars acquired in 2005 at the Observatorio Astronomico Nacional, Mexico, we also observed several standard stars, HD115520 among them. After the reduction this star showed indications of variability. In view of this, a new observing run was carried out in 2006 during which we were able to demonstrate its variability and its nature as a Delta Scuti star. New observations in 2007 permitted us to determine its periodic content with more accuracy. This, along with the uvby-beta photoelectric photometry allowed us to deduce its physical characteristics and pulsational modes.Comment: 6 pages. To be published in Journal of Physics: Conference Series. Proc. of the workshop Helioseismology, Asteroseismology and MHD Connections held in Gottingen, Germany, 20-24 August 200

Clinical practice of language fMRI in epilepsy centers: a European survey and conclusions by the ESNR Epilepsy Working Group

Purpose: To assess current clinical practices throughout Europe with respect to acquisition, implementation, evaluation, and interpretation of language functional MRI (fMRI) in epilepsy patients. Methods: An online survey was emailed to all European Society of Neuroradiology members (n = 1662), known associates (n = 6400), and 64 members of European Epilepsy network. The questionnaire featured 40 individual items on demographic data, clinical practice and indications, fMRI paradigms, radiological workflow, data post-processing protocol, and reporting. Results: A total of 49 non-duplicate entries from European centers were received from 20 countries. Of these, 73.5% were board-certified neuroradiologists and 69.4% had an in-house epilepsy surgery program. Seventy-one percent of centers performed fewer than five scans per month for epilepsy. The most frequently used paradigms were phonemic verbal fluency (47.7%) and audi

Country-report pattern corrections of new cases allow accurate 2-week predictions of COVID-19 evolution with the Gompertz model

Accurate short-term predictions of COVID-19 cases with empirical models allow Health Officials to prepare for hospital contingencies in a two–three week window given the delay between case reporting and the admission of patients in a hospital. We investigate the ability of Gompertz-type empiric models to provide accurate prediction up to two and three weeks to give a large window of preparation in case of a surge in virus transmission. We investigate the stability of the prediction and its accuracy using bi-weekly predictions during the last trimester of 2020 and 2021. Using data from 2020, we show that understanding and correcting for the daily reporting structure of cases in the different countries is key to accomplish accurate predictions. Furthermore, we found that filtering out predictions that are highly unstable to changes in the parameters of the model, which are roughly 20%, reduces strongly the number of predictions that are way-off. The method is then tested for robustness with data from 2021. We found that, for this data, only 1–2% of the one-week predictions were off by more than 50%. This increased to 3% for two-week predictions, and only for three-week predictions it reached 10%

Evaluation of testicular echotexture with Ecotext as a diagnostic method of testicular dysfunction in stallions

This study aimed to assess if Ecotext, a new software for evaluation of testicular echotexture, is a good method for diagnosis of stallions with testicular dysfunction (TD). Relationships between Ecotext parameters and sperm motility and production, testicular volume, and testicular blood flow were also studied. Ecotext provides a total of six echotexture parameters: Ecotext 1 (black pixels), 2 (white pixels) and 3 (grey pixels), and another 3 parameters related to hypoechogenic areas: Ecotext tubular density (ETD), Ecotext tubular diameter (ETd), and Ecotext tubular area (ETA). Stallions (n = 33) were assessed using proven diagnostic techniques (spermiogram, B-mode and Pulse Doppler ultrasound), and subsequent analysis with Ecotext. Animals were classified as “control stallions” (n:21, acceptable semen quality), and “stallions with TD” (n:12, poor semen quality (TM < 60%, PM < 45% and total nº of sperm with PM < 2000 × 106 spz), that were subdivided into “induced TD group” (immunized, anti-GnRH vaccine) and “acquired TD group”. The acquired TD group showed differences in all Ecotext parameters in relation to controls (Ecotext 1:0.11 ± 0.17 vs 2.82 ± 2.52, Ecotext 2:1584.0 ± 575.8 vs 388 ± 368.2, Ecotext 3:134.2 ± 9.26; ETA: 2.14 ± 0.59 vs 5.40 ± 1.90; ETd: 65.66 ± 6.27 vs 86.93 ± 10.65 and ETD: 92.35 ± 11.24 vs 132.10 ± 16.35, p ≤ 0.001). Results suggest acquired TD stallions were suffering testicular degeneration with loss of architecture and function as all Ecotext parameters were altered in relation to controls. Induced TD horses only showed a reduction in ETD (116.2 ± 8.59 vs 132.10 ± 16.35, p ≤ 0.001), despite all sperm parameters being worse. These findings suggested immunized stallions probably only experience an acute loss of testicular functionality and parenchyma architecture is likely not affected since differences in Ecotext parameters with control stallions were not detected. ETD was the best parameter to identify animals with TD (AUC: 0.84, optimal cut-off value of 124.3 seminiferous tubules/cm2). Correlations were found between ETD and Doppler indices (PI: 0.60; RI: 0.47 p ≤ 0.001), total testicular volume (r: 0.48; p ≤ 0.05) and sperm motility (TM:0.51; and PM:0.54; p ≤ 0.001) and production (r:0.51; p ≤ 0.001). In summary, Ecotext could identify changes in testicular echotexture of stallions with TD. Results open the possibility for new research focused on establishing the relationship between Ecotext parameters and histomorphometry features in stallion testes