Synthesis and Characterization of Azo-Guanidine Based Alcoholic Media Naked Eye DNA Sensor

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 x 103, 2.4 x 103 and 0.2 x 103 M-1, for UA1, UA6 and UA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the lambda(max) value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng μl-1 for UA1, UA6 and UA7, respectively

Advanced polymeric/inorganic nanohybrids: An integrated platform for gas sensing applications

Rapid industrial development, vehicles, domestic activities and mishandling of garbage are the main sources of pollutants, which are destroying the atmosphere. There is a need to continuously monitor these pollutants for the safety of the environment and human beings. Conventional instruments for monitoring of toxic gases are expensive, bigger in size and time-consuming. Hybrid materials containing organic and inorganic components are considered potential candidates for diverse applications, including gas sensing. Gas sensors convert the information regarding the analyte into signals. Various polymeric/inorganic nanohybrids have been used for the sensing of toxic gases. Composites of different polymeric materials like polyaniline (PANI), poly (4-styrene sulfonate) (PSS), poly (3,4-ethylene dioxythiophene) (PEDOT), etc. with various metal/metal oxide nanoparticles have been reported as sensing materials for gas sensors because of their unique redox features, conductivity and facile operation at room temperature. Polymeric nanohybrids showed better performance because of the larger surface area of nanohybrids and the synergistic effect between polymeric and inorganic materials. This review article focuses on the recent developments of emerging polymeric/inorganic nanohybrids for sensing various toxic gases including ammonia, hydrogen, nitrogen dioxide, carbon oxides and liquefied petroleum gas. Advantages, disadvantages, operating conditions and prospects of hybrid composites have also been discussed.Rivers, Ports, Waterways and Dredging EngineeringHydraulic EngineeringAerospace Manufacturing Technologie

Data from: Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 × 103, 2.4 × 103 and 0.2 × 103 M−1, for UA1, UA6 and UA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the λmax value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng µl−1 for UA1, UA6 and UA7, respectively

Synthesis, Crystal Structure, and DFT Calculations of 1,3-Diisobutyl Thiourea

1,3-Diisobutyl thiourea was synthesized and characterized by single crystal X-ray diffraction. It gives a monoclinic (α = γ = 90 and β  ≠ 90) structure with the space group P21/c. The unit cell dimensions are a = 11.5131 (4) Å, b = 9.2355 (3) Å, c = 11.3093 (5) Å, α = 90°, β = 99.569° (2), γ = 90°, V = 1185.78 (8) Å3, and Z = 4. The crystal packing is stabilized by intermolecular (N–H⋯S) hydrogen bonding in the molecules. The optimized geometry and Mullikan's charges of the said molecule calculated with the help of DFT using B3LYP-6-311G model support the crystal structure

Ataf Ali - Excell Data from Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (<b>UA1</b>, <b>UA6</b> and <b>UA7</b>) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, ¹H NMR and ¹³C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 x 10³, 2.4 x 10³ and 0.2 x 10³M^-1, for <b>UA1</b>, <b>UA6</b> and <b>UA7</b>, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the <i>λ</i>_max value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0ng µl^-1 for <b>UA1</b>, <b>UA6</b> and <b>UA7</b>, respectively

Supplementary Figure 1 from Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

UV-Visible spectrum of compound of UA6 (50µM) without DNA and with DNA interaction (20µM-140µM) in 80% ethanol, phosphate buffer (0.1M, pH=7.0). The full scan from 200nm to 800nm