Impact of the start-up process on the microbial communities in biocathodes for electrosynthesis

[EN]This study elucidates the impact of the start-up strategies on the microbial communities that evolve on the biofilm of a biocathode. Using reductive start-up potentials and a highly diverse inoculum, this start-up failed to produce any biofilm. When a less species richness inoculum from an anaerobic environment was used with the same reductive initial potential, a specialised biofilm was formed and a highly productive biocathode was developed in terms of acetic acid and also current production. However, using oxidative start-up potential led to rapid electroactive biofilm development, although the final composition of the biofilm was highly dependent on the inoculum used. So, using the diverse RM inoculum, a final specialised biofilm grew on the electrode, also giving high acetate and current generation. However, when using the less species richness AD inoculum, it was found that a nonspecialised biofilm was developed and lower acetic acid production was found. Importantly, a higher specialisation of the biofilm leads to an improvement in acetate generation, probably due to lowered influence of undesirable secondary methabolic pathways. Moreover, it has been shown that the coupling of H2 producing bacteria and acetic acid bacteria play an important role in acetate productionSIThis research was possible thanks to the financial support of the ‘Ministerio de Economía y Competitividad’ project ref: CTQ2015-68925-R, cofinanced by FEDER funds. Raúl Mateos thanks the Spanish ‘Ministerio de Educación, Cultura y Deporte’ for the FPU Grant (FPU14/01573). Ana Sotres thanks the regional ‘Junta de Castilla y León’ for the postdoctoral contract associated with project ref: LE060U16

Integrating microbial electrochemical technologies with anaerobic digestion to accelerate propionate degradation

[EN] The aim of this study is to evaluate the integration of microbial electrochemical technologies (MET) with anaerobic digestion (AD) to overcome AD limitations caused by propionate accumulation. The study focuses on understanding to what extent the inoculum impacts on the behaviour of the integrated systems (AD-MET) from the perspective of propionate degradation, methane production and microbial population dynamics. Three different inocula were used: two from environmental sources (anaerobic sludge and river sediment) and another one from a pre-enriched electroactive consortium adapted to propionate degradation. Contrary to expectations, the reactor inoculated with the pre-enriched consortium was not able to maintain its initial good performance in the long run, and the bioelectrochemical activity collapsed after three months of operation. On the other hand, the reactor inoculated with anaerobic sludge, although it required a relatively longer time to produce any observable current, was able to maintain an electrogenic activity operation (0.8 A m−2), whilst showcasing the positive contribution of AD-MET integration into tackling propionate accumulation and enhancing methane yield (338 mL gCOD−1). However, it must also be highlighted that from a purely energetic point of view the AD-MET was not favourable.SIMinisterio de Economía y CompetitividadJunta de Castilla y LeónEnte Regional de la Energía de Castilla y Leo

Comparison of Activation Methods for 3D-Printed Electrodes for Microbial Electrochemical Technologies

Supplementary Materials: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/app12010275/s1, Figure S1. Current density profiles of two successive cycles at the end of a 60 days period that allowed for the development of a stable biofilm; Table S1: Fitted parameters to EC 1 (DMF and acetone electrodes); Table S2: Fitted parameters to EC 2 (control and electrochemical-treated electrodes); Figure S2. Equivalent circuits for abiotic electrode essays’ modellization.1) DMF and acetone 2) Control and electrochemical treatment.[EN] Three-dimensional printing could provide flexibility in the design of a new generation of electrodes to be used in microbial electrochemical technologies (MET). In this work, we demonstrate the feasibility of using polylactic acid (PLA)/graphene—a common 3D-printing material—to build custom bioelectrodes. We also show that a suitable activation procedure is crucial to achieve an acceptable electrochemical performance (plain PLA/graphene bioanodes produce negligible amounts of current). Activation with acetone and dimethylformamide resulted in current densities similar to those typically observed in bioanodes built with more conventional materials (about 5 Am−2). In addition, the electrodes thus activated favored the proliferation of electroactive bacteria.SIMinisterio de Economía y CompetitividadEnte Regional de la Energía de Castilla y LeonThis research was funded by “Ministerio de Economía y Competitividad (Gobierno de España) grant number: PID2020-115948RB-I00 (MINECO/FEDER, EU) and “Ente Regional de la Energía de Castilla y Leon”, grant number: EREN_2019_L3_ULE

Assessing anodic microbial populations and membrane ageing in a pilot microbial electrolysis cell

Bioelectrochemical system[EN] First large-scale experiences of bioelectrochemical systems (BES) are underway. However, there is still little knowledge on how the different elements that integrate a BES behave in near real-life conditions. This paper aims at assessing the impact of long-term operation on the cation exchange membrane and on the anodic biofilm of two 16 L Microbial Electrolysis Cells (MEC) designed for hydrogen production and ammonia recovery from pig slurry. Membrane deterioration was examined by physical, chemical and microscopy techniques at different locations, revealing a strong attachment of microorganisms and a significant decay in membrane properties such as ion exchange capacity and thermal stability. Anode microbial communities did not show a dramatic shift in the eubacteria composition at different sampling areas, although the relative abundance of some bacterial groups showed a clear vertical stratification. After 100 days of continuous operation, MEC performance did not declined significantly maintaining ammonium transport rates and H2 production rates of 15.3 gN d−1 m−2 and 0.2 LH2·L−1reactor·d−1 respectively.SIMinisterio de Economía y CompetitividadJunta de Castilla y LeónMinisterio de Educación, Cultura y Deport

Degradation of 2-mercaptobenzothizaole in microbial electrolysis cells: Intermediates, toxicity, and microbial communities

[EN] The compound 2-mercaptobenzothizaole (MBT) has been frequently detected in wastewater and surface water and is a potential threat to both aquatic organisms and human health (its mutagenic potential has been demonstrated). This study investigated the degradation routes of MBT in the anode of a microbial electrolysis cell (MEC) and the involved microbial communities. The results indicated that graphene-modified anodes promoted the presence of more enriched, developed, and specific communities compared to bare anodes. Moreover, consecutive additions of the OH substituent to the benzene ring of MBT were only detected in the reactor equipped with the graphene-treated electrode. Both phenomena, together with the application of an external voltage, may be related to the larger reduction of biotoxicity observed in the MEC equipped with graphene-modified anodes (46.2 eqtox∙m−3 to 27.9 eqtox∙m−3).SIThis research was possible thanks to the financial support by ‘Consejería de Educación de la Junta de Castilla y León’ (ref: LE320P18), a project co-financed by FEDER funds. R. M. Alonso thanks the University of León for the predoctoral contract. M. Canle acknowledges financial support from the Ministerio de Economía y Competitividad (Spain) through project CTQ2015-71238-R (MINECO/FEDER), and regional government Xunta de Galicia (project GPC ED431B 2017/59), respectively

Characterization of Anaerobic Biofilms Growing on Carbon Felt Bioanodes Exposed to Air

Supplementary Materials: The following are available online at https://www.mdpi.com/2073-4344/10/11/1341/s1, Figure S1: Dissolved oxygen profile. Rejected because the data acquisition system was accidentally interrupted at the end of the experiment; Figure S2: Another dissolved oxygen profile. Rejected because the data acquisition system was accidentally interrupted at the end of the experiment.[EN] The role of oxygen in anodic biofilms is still a matter of debate. In this study, we tried to elucidate the structure and performance of an electrogenic biofilm that develops on air-exposed, carbon felt electrodes, commonly used in bioelectrochemical systems. By simultaneously recording the current density produced by the bioanode and dissolved oxygen concentration, both inside and in the vicinity of the biofilm, it was possible to demonstrate the influence of a protective aerobic layer present in the biofilm (mainly formed by Pseudomonas genus bacteria) that prevents electrogenic bacteria (such as Geobacter sp.) from hazardous exposure to oxygen during its normal operation. Once this protective barrier was deactivated for a long period of time, the catalytic capacity of the biofilm was severely affected. In addition, our results highlighted the importance of the material’s porous structure for oxygen penetration in the electrode.SIJunta de Castilla y LeonThis research was possible thanks to the financial support by ‘Consejería de Educación de la Junta de Castilla y León’ (ref: LE320P18), a project co-financed by FEDER funds. R. M. Alonso thanks the University of León for his predoctoral contract

Complete arsenic removal from water using biocatalytic systems based on anaerobic films grown on carbon fibers

[EN] Arsenic is a hazardous metalloid with potentially negative impacts on both the environment and human health. Current methods of arsenic remediation are expensive and can cause secondary contamination. In this paper we explore the potential of using bioelectrochemical systems (a group of environmentally friendly bio-based technologies with great potential for bioremediation and waste valorisation) for arsenic removal. Previous studies have reported that the spontaneous oxidation of As(III) to As(V) was completely realized in bioelectrochemical systems, however, any of the them succeeded in removing the total arsenic concentration. This study demonstrates that not only it is possible to oxidize As(III) to As(V), but also the total elimination of arsenic can be achieved as the result of intracellular accumulation.SIThis research was possible thanks to the financial support by "Consejeria de Educacion de la Junta de Castilla y Leon" (ref: LE320P18), a project co-financed by FEDER funds. R. M. Alonso thanks the University of Leon for his predoctoral contract. F. Ivars-Barcelo acknowledges the& Spanish "Agencia Estatal de Investigacion" for the Ramon y Cajal excellence grant (Ref.: RYC2020-029470-I/AEI/10.13039/501100011033)

Galaxy bias in the era of LSST: perturbative bias expansions

Upcoming imaging surveys will allow for high signal-to-noise measurements of galaxy clustering at small scales. In this work, we present the results of the LSST bias challenge, the goal of which is to compare the performance of different nonlinear galaxy bias models in the context of LSST Y10 data. Specifically, we compare two perturbative approaches, Lagrangian perturbation theory (LPT) and Eulerian PT (EPT) to two variants of Hybrid Effective Field Theory (HEFT), with our fiducial implementation of these models including terms up to second order in the bias expansion as well as nonlocal bias and deviations from Poissonian stochasticity. We consider different simulated galaxy samples and test the performance of the bias models in a tomographic joint analysis of LSST-Y10-like galaxy clustering, galaxy-galaxy-lensing and cosmic shear. We find both HEFT methods as well as LPT and EPT combined with non-perturbative predictions for the matter power spectrum to yield unbiased constraints on cosmological parameters up to at least a maximal scale of $k_{\mathrm{max}}=0.4 \; \mathrm{Mpc}^{-1}$ for all samples considered, even in the presence of assembly bias. While we find that we can reduce the complexity of the bias model for HEFT without compromising fit accuracy, this is not generally the case for the perturbative models. We find significant detections of non-Poissonian stochasticity in all cases considered, and our analysis shows evidence that small-scale galaxy clustering predominantly improves constraints on galaxy bias rather than cosmological parameters. These results therefore suggest that the systematic uncertainties associated with current nonlinear bias models are likely to be subdominant compared to other sources of error for tomographic analyses of upcoming photometric surveys, which bodes well for future galaxy clustering analyses using these high signal-to-noise data. [abridged]Comment: 47 pages, 19 figures, 1 table, to be submitted to JCA

Blood donations and transfusions during the COVID-19 pandemic in Spain: Impact according to autonomous communities and hospitals

Worldwide, the COVID-19 pandemic has caused a decline in blood donations, between 30% and 70% in some of the most affected countries. In Spain, during the initial eight weeks after the State of Emergency was decreed on 14 March 2020, in the weekly reports of the Health Ministry, an average decrease of 20% was observed between 11 and week 25 compared with the 2018 donation. We aimed to investigate the impact of the COVID-19 pandemic on blood donations and blood distribution in four autonomous communities, and to explore the evolution of the consumption of blood components (BCs) in ten hospitals of six autonomous communities. We performed a prospective study of grouped cohorts on the donation and distribution of blood in four regional transfusion centers in four autonomous communities in Spain, and a retrospective study of the consumption of blood components in ten hospitals in six autonomous communities. Regarding donations, there was no significant decrease in donations, with differences between autonomous communities, which started between 1 and 15 March 2020 (−11%). The increase in donations in phase II (from 26 May 2020) stands out. Regarding consumption, there was a significant reduction in the consumption of packed red blood cells (RBCs) (24.5%), plasma (45.3%), and platelets (25.3%) in the central period (16 March–10 May). The reduction in the consumption of RBCs was significant in the period from 1–15 March. Conclusions: The COVID-19 pandemic has affected the donation and consumption of BCs

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality