Potential risk of Artemia sp as a transmission vector of lymphocystis disease virus (LCDV)

4 páginas, 1 figura. XI Congreso Nacional de Acuicultura (Vigo, 24-28 septiembre 2007). Ed. Antonio Cerviño Eiroa, Alejandro Guerra Díaz y Carmen Pérez Acosta.[EN] Cysts and naupliis of Artemia sp. were analysed. Specific nested-PCR revealed lymphocystis disease virus (LCDV) genome. Infective viral particle has been observed by CPE development in inoculated cell cultures. Viral genome was found by in situ hybridization in nauplii and adults of natural infected artemia, as in nauplii of bath challenged artemia. No morphological damages have been observed. Artemia is a bio-accumulator of fish pathogens, with a possible role as environmental reservoir of fish pathogens. These results have shown the risk of artemia as a source of viral pathogens to fish larvae.[ES] En el presente trabajo se han realizado estudios virológicos en distintos lotes de quistes comerciales de artemias utilizados como alimento de larvas de peces marinos en piscifactorías. Mediante nested-PCR se ha detectado genoma del virus de linfocistis (LCDV) en homogeneizados de quistes y nauplios de artemia. La existencia de partículas víricas infectivas en estos homogeneizados se ha demostrado mediante la aparición de efectos citopáticos en cultivos celulares. Los ensayos de hibridación in situ han demostrado la existencia del LCDV en artemias infectadas naturalmente, así como en artemias inoculadas mediante baño, sin observarse alteraciones morfológicas. Las artemias actúan por tanto como bioacumuladores, pudiendo desempeñar un papel importante como reservorios ambientales de patógenos de peces. Estos resultados ponen de manifiesto el riesgo potencial de las artemias como fuente de patógenos víricos en estadios larvarios.Irene Cano es contratada postdoctoral en el ICMAN.CSIC dentro del Fondo social europeo- I3P-CSIC, en el marco del proyecto AGL2006-17777-C03-02/ACU (IP: Carmen Sarasquete).Peer reviewe

Visual Ontology Cleaning: Cognitive Principles and Applicability

In this paper we connect two research areas, the Qualitative Spatial Reasoning and visual reasoning on ontologies. We discuss the logical limitations of the mereotopological approach to the visual ontology cleaning, from the point of view of its formal support. The analysis is based on three different spatial interpretations wich are based in turn on three different spatial interpretations of the concepts of an ontology.Ministerio de Educación y Ciencia TIN2004-0388

In vivo expansion of a CD9+ decidual-like NK cell subset following autologous hematopoietic stem cell transplantation.

Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA-sequencing on NK cells before and after autoHSCT. Results showed profound changes in the gene expression profile of NK cells immediately after autoHSCT. Several biological processes including cell cycle, DNA replication and the mevalonate pathway were enriched. Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile, including the expression of CD9. By using multiparametric flow cytometry, we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells. These results provide insights into the physiopathology of NK cells during their reconstitution after autoHSCT.Supported by the following grants: AECC-Spanish Association Against Cancer (PROYE16074- BORR) and Health Department, Basque Government (2021333006). GA-P is the recipient of a predoctoral contract funded by AECC-Spanish Association Against Cancer (PRDVZ21440ASTA). DP-A is a recipient of a fellowship from the AECC-Spanish Association Against Cancer (PPLAB212164POLA), AA-I and GA-P are recipient of a fellowship from the Jesús de Gangoiti Barrera Foundation (FJGB20/007, FJGB21/001 and FJBG21/005). IT is recipient of a predoctoral contract funded by the Department of Education, Basque Government (PRE_2021_2_0215). OZ is the recipient of a postdoctoral contract funded by ‘‘Instituto de Salud Carlos III-Contratos Sara Borrell 2017 (CD17/00128)’’ and the European Social Fund (ESF)-The ESF invests in your future. FB is an Ikerbasque Research Professor, Ikerbasque, Basque Foundation for Science.S

Definition of a list of fish diseases to aid health management in Spain

Trabajo presentado en la 14th EAFP International Conference (European Association of Fish Pathologists), celebrada en Praga (República Checa), del 14 al 19 de septiembre de 2009An expert working group used a risk ranking technique to compile a list of fish disease hazards (pathogens) of relevance to Spanish aquaculture. It was possible to divide the list into three groups: I-high national risk; II-regional risk; III-low risk. The three groups were as follows: Group I (high risk) Aphanomyces invadans (EUS)**, spring viraemia of carp virus (SVCV), koi herpes virus (KHV)¿ and infectious haematopoietic necrosis virus (IHNV)¿ Group II (regional risk) Enteromyxum spp. (leei and scophthalmi), Aquabirnaviridae (incl. IPNV), viral encephalopathy and retinopathy virus (VERV), Streptococcus iniae, Philasterides dicentrarchi and Aeromonas salmonicida (in the marine environment). Group III (low risk) Sparicotyle chrysophrii/Microcotylidae, Flavobacterium maritimus, Photobacterium piscicida, Togaviridae, Sphaerospora testicularis, Edwardsiella tarda, Birnavirus (no-EVE), Lactococcus garviae, viral haemorrhagic septicaemia virus (VHSV)¿, Tenacibaculum maritimum, epizootic haematopoietic necrosis virus (EHNV)**, Renibacterium salmoninarum (BKD) and Gyrodactylus salaris. The exercise formed part of a project concerning aquaculture health management (Jacumar-GESAC¿) in Spain and is being used to help define sampling plans for disease monitoring using epidemiological and risk-based criteria. **The diseases caused by these pathogens are notifiable and exotic according to Directive 2006/88/EC ¿The diseases caused by these pathogens are notifiable and non-exotic according to Directive 2006/88/EC ¿Plan Nacional de Cultivos Marinos; Gestión sanitaria de la acuicultura: Adaptación a la nueva normativa (GESAC) ¿ financed by the Junta Nacional Asesora de Cultivos Marinos (Jacumar

A crowdsourcing database for the copy-number variation of the spanish population

Background: Despite being a very common type of genetic variation, the distribution of copy-number variations (CNVs) in the population is still poorly understood. The knowledge of the genetic variability, especially at the level of the local population, is a critical factor for distinguishing pathogenic from non-pathogenic variation in the discovery of new disease variants. Results: Here, we present the SPAnish Copy Number Alterations Collaborative Server (SPACNACS), which currently contains copy number variation profiles obtained from more than 400 genomes and exomes of unrelated Spanish individuals. By means of a collaborative crowdsourcing effort whole genome and whole exome sequencing data, produced by local genomic projects and for other purposes, is continuously collected. Once checked both, the Spanish ancestry and the lack of kinship with other individuals in the SPACNACS, the CNVs are inferred for these sequences and they are used to populate the database. A web interface allows querying the database with different filters that include ICD10 upper categories. This allows discarding samples from the disease under study and obtaining pseudo-control CNV profiles from the local population. We also show here additional studies on the local impact of CNVs in some phenotypes and on pharmacogenomic variants. SPACNACS can be accessed at: http://csvs.clinbioinfosspa.es/spacnacs/. Conclusion: SPACNACS facilitates disease gene discovery by providing detailed information of the local variability of the population and exemplifies how to reuse genomic data produced for other purposes to build a local reference database.This work is supported by Grants PID2020-117979RB-I00 from the Spanish Ministry of Science and Innovation; by the Institute of Health Carlos III (project IMPaCT-Data, exp. IMP/00019, IMP/00009 and PI20/01305), co-funded by the European Union, European Regional Development Fund (ERDF, “A way to make Europe”)

Credit Information Sharing and Loan Default in Developing Countries: The Moderating Effect of Banking Market Concentration and National Governance Quality

Departing from the existing literature, which associates credit information sharing with improved access to credit in advanced economies, we examine whether credit information sharing can also reduce loan default rate for banks domiciled in developing countries. Using a large dataset covering 879 unique banks from 87 developing countries from every continent, over a nine-year period (i.e., over 6,300 observations), we uncover three new findings. First, we find that credit information sharing reduces loan default rate. Second, we show that the relationship between credit information sharing and loan default rate is conditional on banking market concentration. Third, our findings suggest that governance quality at the country level does not have a strong moderating role on the effect of credit information sharing on loan default rate

Partnering Universities and Companies in Russia: Effects of New Government Initiative

The paper presents the results of 2-year survey conducted in 2011-2012 among Russian universities and companies who jointly implement R&D projects aimed at development of high-tech manufacturing. The joint projects represent a new government instrument to stimulate the development of linkages between universities and companies by giving matching grants for R&D to companies with obligation to order R&D to a university-partner. The objectives of the survey included analysis of motivation for cooperation both from side of universities and companies; primary effects and side-effects of such initiative; changes that may be introduced to the government regulations concerning matching grants. Total 38 teams were surveyed. Our findings show that major motivations from side of universities were access to new practical research tasks from companies, selection of most competitive teams of researchers capable to work with companies, and strengthening reputation in business environment. Companies were interested in getting government funding in order to solve their technological problems; to strengthen, due cooperation with universities, their research capacity, and to use modern research infrastructure located at universities. The analysis allowed identification of the major effects of the matching grants mechanism. They included: strengthening of university orientation towards solving practical tasks which are of interest to business; institutionalization of relations between universities and business in the sphere of innovation activity; broadening of research cooperation and the formation of research consortiums; harmonization of research and educational tasks in universities, and orientation of the parties towards continuing cooperation in the innovation sphere