Efeitos do treinamento muscular inspiratório e dos exercícios respiratórios em crianças com asma: revisão sistemática

Introduction: Asthma is characterized by a narrowing and inflammation of the bronchi, with symptoms of dyspnea, fatigue and exercise limitation. Physical therapy includes inspiratory muscle training and breathing exercises, given that an increase in inspiratory muscle strength and resistance can improve the symptoms of the disease. Objective: To describe the effects of inspiratory muscle training (IMT) and breathing exercises in children with asthma. Methods: This is a systematic review of the literature using the Cochrane, PubMed Scopus e Web of Science databases. The following descriptors were used: asthma, inspiratory muscle training, breathing exercises and child in Portuguese, English and Spanish. Two independent evaluators screened studies that used breathing exercises and IMT in children with asthma. Results: Of a total of 312 titles, eight studies were included, of which six are randomized clinical trials and two are observational studies All the studies included breathing exercises, with the objective of adjusting breathing patterns and pulmonary ventilation, reducing pulmonary hyperinflation, bronchospasm and sensation of dyspnea. However, as these exercises were not performed solely, the effects of this intervention could not be verified. Two studies performed IMT and showed an increase in maximal respiratory pressure. Conclusion: Breathing exercises are widely used in clinical practice as part of the management of asthma in children; however it is not possible to measure the effects in this population. IMT seems to improve inspiratory and expiratory muscle strength, but its indication in the pediatric population is not a standard procedure.Introdução: Asma é caracterizada por estreitamento e inflamação dos brônquios, com sintomas de dispneia, fadiga e limitação aos exercícios. O tratamento fisioterapêutico engloba treinamento muscular inspiratório e exercícios respiratórios, pois o aumento da força e resistência da musculatura inspiratória podem melhorar os sintomas da doença. Objetivo: Descrever os efeitos do treinamento muscular inspiratório (TMI) e dos exercícios respiratórios na criança com asma. Método: Revisão sistemática da literatura conduzida nas bases de dados Cochrane, PubMed, Scopus e Web of Science. Utilizou-se os descritores: asma, treinamento muscular inspiratório, exercícios respiratórios e criança e seus correlatos em inglês e espanhol. Dois avaliadores independentes elencaram estudos que realizaram intervenção com exercícios respiratórios e TMI na criança com asma. Resultados: De um total de 312 títulos, foram incluídos oito estudos, dos quais seis são ensaios clínicos randomizados e dois observacionais. Todos os trabalhos incluíram exercícios respiratórios, com o objetivo de adequar o padrão respiratório e a ventilação pulmonar, reduzir a hiperinsuflação pulmonar, o broncoespasmo e a sensação de dispneia, porém como não foram realizados de forma isolada, comprometeu a verificação dos efeitos dessa intervenção. Dois estudos realizaram TMI e evidenciaram aumento nas pressões respiratórias máximas. Conclusão: Exercícios respiratórios são muito utilizados na prática clínica como parte do manejo da criança com asma, entretanto ainda não é possível mensurar seus efeitos nessa população. O TMI parece melhorar a força muscular inspiratória e expiratória, mas sua indicação na população pediátrica ainda não é rotineira

Mechanisms of vesicular stomatitis virus inactivation by protoporphyrin ix, zinc- protoporphyrin ix, and mesoporphyrin ix

© 2017 American Society for Microbiology. All Rights Reserved.Virus resistance to antiviral therapies is an increasing concern that makes the development of broad-spectrum antiviral drugs urgent. Targeting of the viral envelope, a component shared by a large number of viruses, emerges as a promising strategy to overcome this problem. Natural and synthetic porphyrins are good candidates for antiviral development due to their relative hydrophobicity and pro-oxidant character. In the present work, we characterized the antiviral activities of protoprophyrin IX (PPIX), Zn-protoporphyrin IX (ZnPPIX), and mesoporphyrin IX (MPIX) against vesicular stomatitis virus (VSV) and evaluated the mechanisms involved in this activity. Treatment of VSV with PPIX, ZnPPIX, and MPIX promoted dose-dependent virus inactivation, which was potentiated by porphyrin photoactivation. All three porphyrins inserted into lipid vesicles and disturbed the viral membrane organization. In addition, the porphyrins also affected viral proteins, inducing VSV glycoprotein cross-linking, which was enhanced by porphyrin photoactivation. Virus incubation with sodium azide and α-tocopherol partially protected VSV from inactivation by porphyrins, suggesting that singlet oxygen (1O2) was the main reactive oxygen species produced by photoactivation of these molecules. Furthermore, 1O2 was detected by 9,10-dimethylanthracene oxidation in photoactivated porphyrin samples, reinforcing this hypothesis. These results reveal the potential therapeutic application of PPIX, ZnPPIX, and MPIX as good models for broad antiviral drug design.Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ; Brazil; grant number E-26/201.167/2014), the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq; Brazil; grant number 306669/2013-7), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES; Brazil; grant number CsF 171/2012), the Fundacao para a Ciencia e Tecnologia-Ministério da Educação e Ciência (FCT-MEC; Portugal; project HIVERA/0002/2013), and Marie Skłodowska-Curie Actions (MSCA; European Commission project INPACT 644167). C.C.-O. acknowledges a Science without Borders postdoctoral fellowship from CAPES (171/2012), and J.M.F. acknowledges an FCT-MEC Ph.D. fellowship (SFRH/BD/70423/2010)info:eu-repo/semantics/publishedVersio

Determinação das curvas de secagem das sementes de andiroba em secador solar

The oil of crabwood is commonly extracted in Amazon by traditional or by pressing method. The extraction efficiency is related to the heating and water content of the seeds. Thus, the determination of a drying model that represent satisfactorily the experimental data is of paramount importance to minimize the changes introduced by the process, consequently obtaining a quality product. The objective of this study was to describe the drying kinetics of seeds of crabwood as well as adjust the mathematical models to the experimental data, using solar dryer. The coefficient of determination, the magnitude of the mean relative error and standard deviation of the estimate was used as the criterion of fit of mathematical models. Drying in a shorter period of time (14 days) of Carapa surinamensis to reach the water equilibrium content (12.28%) may be attributed to the smaller size of the seeds and the greater amount of oil compared to Carapa guianensis. Logarithmic and Midilli et al. were the model that best fitted the experimental data for seeds of both species of Andiroba. © 2015, Departamento de Engenharia Agricola - UFCG/Cnpq. All rights reserved

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery