RANS K-? SIMULATION OF 2D TURBULENT NATURAL CONVECTION IN AN ENCLOSURE WITH HEATING SOURCES

ABSTRACT: This study is conducted to investigate turbulent natural convection flow in an enclosure with thermal sources using the low-Reynolds number (LRN) k-? model. This enclosure has a cold source with temperature Tc and a hot source with temperature Th as thermal sources, other walls of the enclosure are adiabatic. The aim of this study is to predict the effect of change in Rayleigh number, repositioning of cold and hot sources, and thermal sources aspect ratio on the flow field, temperature, and rate of heat transfer. To achieve this aim, the equations of continuity, momentum, energy, turbulent kinetic energy, and kinetic energy dissipation are employed in the case of 2D turbulence with constant thermo-physical properties except the density in the buoyancy term (Boussinesq approximation). To numerically solve these equations, the finite volume method and SIMPLE algorithm are used. According to the modeling results, the most optimal temperature distribution in the enclosure is seen when the hot source is below the cold source. With decreasing distance between hot and cold sources, heat transfer rate increases. The maximal heat transfer rate is derived via study of the heating sources aspect ratio. In constant positions of cold and hot sources on a wall, the heat transfer rate increases with increasing Rayleigh number (Ra=109-1011). ABSTAK: Kajian ini dijalankan bagi mengkaji perubahan semula jadi aliran perolakan dalam tempat tertutup dengan sumber haba menggunakan model k-? nombor Reynolds-rendah (LRN). Bekas tertutup ini mempunyai dua sumber haba iaitu sumber sejuk dengan suhu Tc dan sumber panas dengan suhu Th, manakala dinding lain bekas ini adalah adiabatik. Tujuan kajian ini adalah bagi mengesan perubahan nombor Rayleigh, mengubah sumber sejuk dan panas dan nisbah sumber haba kepada kawasan aliran, suhu dan halaju perubahan haba. Bagi mencapai tujuan tersebut, persamaan sambungan, momentum, tenaga, tenaga kinetik perolakan, dan pengurangan tenaga kinetik telah dilaksanakan dalam kes perolakan 2D dengan sifat fizikal-haba berterusan (malar) kecuali isipadu terma keapungan (anggaran Boussinesq). Bagi menyelesaikan persamaan ini secara berangka, kaedah isipadu terhad dan algorithma MUDAH telah digunakan. Berdasarkan keputusan model, suhu distribusi optimal dalam bekas tertutup dilihat apabila sumber panas adalah kurang daripada sumber sejuk. Dengan pengurangan jarak antara sumber panas dan sejuk, kadar pertukaran haba meningkat. Kadar pertukaran haba maksima telah diperoleh melalui kajian nisbah  aspek sumber pemanasan. Kadar pertukaran haba bertambah dengan bertambahnya nombor Rayleigh  (Ra=109-1011), pada posisi tetap sumber sejuk dan panas pada dinding bekas

Study the Molecular Association between a Deletion Mutation in CHEK2 gene (5395 bp) and Breast Cancer

Background & Objectives: Breast cancer is the most common cancer among women and the second most common cause of cancer death. Genetic factors play an important role in the development of breast cancer. Among these genetic factors, CHEk2 (checkpoint kinase 2) gene, as a tumor suppressor gene, plays a critical role in DNA repair. Germline mutations in CEHK2 result in the loss of this feature. One of the mutations in CHEK2 gene is a 5395 bp deletion mutation which has been associated with the increasing risk of Breast Cancer in some populations in the world.  In the present study, we investigated the association between a 5395 bp deletion mutation in CHEK2 gene and the risk of Breast Cancer in the women of an Iranian population. Methods: Pathologic information of 38 cases under the age of 45 and 62 cases over the age of 45 referring to surgery ward of Milad Hospital in Tehran were extracted. 100 healthy controls were included in the study as well. After obtaining informed consent, 5 mL whole blood was taken DNA was successfully isolated. Multiplex PCR was used to investigate the association between a 5395bp deletion mutation in CHEK2 gene and increasing risk of Breast Cancer among patients. Results: The 5395bp deletion mutation in CHEK2 gene was not found in any of the participating groups of patients or heathy controls. Conclusion: The present study revealed that there is no significant relation between increasing the risk of Breast Cancer and bearing large deletion mutation in exon 9 and exon 10 of CHECK2 gene

Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli

ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aimed to provide a reproducible method for recombinant production of the smallest isoform of IL-24 (sIL-24). The Structure of sIL-24 was analyzed using bioinformatics tools (I-TASSER, Prosa, RAMPAGE and SPDBV version 4.1). The DNA sequence encoding sIL-24 was chemically synthesized and sub-cloned into the pET-32a (+) vector for further protein expression in Escherichia coli BL21 (DE3) strain. Upon IPTG induction, sIL-24 peptide was expressed as a thioredoxin fusion protein. The recombinant sIL-24 was released from the fusion by TEV protease cleavage followed by nickel affinity chromatography. The yield of the purified sIL-24 was estimated about 380 μg/ml. MTT assay showed that sIL-24 peptide inhibited the proliferation of PC-3 cancer cells more effectively than full length IL-24 protein, while none affect the survival of MRC-5 normal cells. These results indicate that the presented expression system is an efficient system for the production of small functional recombinant sIL-24 peptide.This functional peptide may have cancer therapeutic application

Evaluation of the effect of blood contamination on the compressive strength of MTA modified with hydration accelerators

Objectives This study was performed to evaluate the effect of blood contamination on the compressive strength (CS) of Root MTA (RMTA) modified with Calcium chloride (CaCl2) and Disodium hydrogen phosphate (Na2HPO4) as setting accelerators over time. Materials and Methods A total of 110 cylindrical specimens of RMTA were divided into 6 experimental groups as follows: Group1, RMTA; Group 2, RMTA modified with CaCl2 (RMTA-C); Group 3, RMTA modified with Na2HPO4 (RMTA-N); Group 4, RMTA contaminated with blood; Group 5, RMTA-C contaminated with blood; Group 6, RMTA-N contaminated with blood. The CS of specimens in all groups was evaluated after 3 hr, 24 hr, and 1 wk. In the modified groups (groups 2, 3, 5, and 6) the CS of five specimens per group was also evaluated after 1 hr. Results Blood contamination significantly reduced the CS of all materials at all time intervals (p < 0.05). After 3 hr, the CS of specimens in the RMTA groups (with and without blood contamination) was significantly lower than those in the RMTA-C and RMTA-N groups (p < 0.05). The CS values were not significantly different at the other time intervals. In all groups, the CS of specimens significantly increased over time (p < 0.05). Conclusions Blood contamination decreased the CS of both original and accelerated RMTA