142 research outputs found
UBC-Nepal expedition: markedly lower cerebral blood flow in high-altitude Sherpa children compared with children residing at sea level
Developmental cerebral hemodynamic adaptations to chronic high-altitude exposure, such as in the Sherpa population, are largely unknown. To examine hemodynamic adaptations in the developing human brain, we assessed common carotid (CCA), internal carotid (ICA), and vertebral artery (VA) flow and middle cerebral artery (MCA) velocity in 25 (9.6 ± 1.0 yr old, 129 ± 9 cm, 27 ± 8 kg, 14 girls) Sherpa children (3,800 m, Nepal) and 25 (9.9 ± 0.7 yr old, 143 ± 7 cm, 34 ± 6 kg, 14 girls) age-matched sea level children (344 m, Canada) during supine rest. Resting gas exchange, blood pressure, oxygen saturation and heart rate were assessed. Despite comparable age, height and weight were lower (both P < 0.01) in Sherpa compared with sea level children. Mean arterial pressure, heart rate, and ventilation were similar, whereas oxygen saturation (95 ± 2 vs. 99 ± 1%, P < 0.01) and end-tidal Pco2 (24 ± 3 vs. 36 ± 3 Torr, P < 0.01) were lower in Sherpa children. Global cerebral blood flow was ∼30% lower in Sherpa compared with sea level children. This was reflected in a lower ICA flow (283 ± 108 vs. 333 ± 56 ml/min, P = 0.05), VA flow (78 ± 26 vs. 118 ± 35 ml/min, P < 0.05), and MCA velocity (72 ± 14 vs. 88 ± 14 cm/s, P < 0.01). CCA flow was similar between Sherpa and sea level children (425 ± 92 vs. 441 ± 81 ml/min, P = 0.52). Scaling flow and oxygen uptake for differences in vessel diameter and body size, respectively, led to the same findings. A lower cerebral blood flow in Sherpa children may reflect specific cerebral hemodynamic adaptations to chronic hypoxia
Impact of prolonged sitting on vascular function in young girls
Excessive sedentary behaviour has serious clinical and public health implications; however, the physiological changes that accompany prolonged sitting in the child are not completely understood. Herein, we examined the acute effect a prolonged period of sitting has upon superficial femoral artery function in 7- to 10-year-old girls and the impact of interrupting prolonged sitting with exercise breaks. Superficial femoral artery endothelium-dependent flow-mediated dilatation, total shear rate, anterograde and retrograde shear rates and oscillatory shear index were assessed before and after two experimental conditions: a 3 h uninterrupted period of sitting (SIT) and a 3 h period of sitting interrupted each hour with 10 min of moderate-intensity exercise (EX). A mixed-model analysis of variance was used to compare between-condition and within-condition main effects, controlling for the within-subject nature of the experiment by including random effects for participant. Superficial femoral artery endothelium-dependent flow-mediated dilatation decreased significantly from pre- to post-SIT (mean difference 2.2% flow-mediated dilatation; 95% confidence interval = 0.60–2.94%, P < 0.001). This relative decline of 33% was abolished in the EX intervention. Shear rates were not significantly different within conditions. Our data demonstrate the effectiveness of short but regular exercise breaks in offsetting the detrimental effects of uninterrupted sitting in young girls
An Innovative Database for Epidemiological Field Studies of Neglected Tropical Diseases
The neglected tropical diseases (NTDs) are of major public health importance, accounting for 56.6 million disability-adjusted life years (DALYs), which places them sixth out of the ten leading causes of life years lost to disability and premature death [1]. These diseases are prominent in the developing world where there is low income, poor hygiene, and inadequate sanitation [1],[2]. Recent targeting of these diseases for large-scale control programs by the World Health Organization [3] is likely to increase the number of epidemiological field studies requiring valid and reliable data, in order to determine the most appropriate strategies for control. In order to ensure a control strategy is effective and appropriate, the data need to be of a high standard, and as a result, epidemiological field studies require a rigorous and systematic approach to data management. Recent publications by Ali et al. [4] and Roberts et al. [5] stress that the importance of data management is often underestimated in such studies, with greater emphasis instead placed on the study design, data collection, and data analysis [4],[5]. This can result in an ad hoc approach to data management that ultimately affects the reliability and validity of the data collected and increases the workload involved in data cleaning. There are additional difficulties in developing countries in the collection, entry, management, and analysis of high-quality data, mainly due to limited infrastructure and capacity [4]-[7], which can exacerbate the problems associated with ensuring effective and reliable data management. We undertook an epidemiological study of the transmission dynamics of Schistosoma japonicum in China [8] that necessitated a rigorous approach to the collection and management of an extensive dataset. Some technical and conceptual constraints were encountered as the data management protocols in place were designed for the monitoring and control of schistosomiasis, rather than for the evaluation of a complex epidemiological study, requiring expertise in the principles and practice of data management. Language barriers provided additional challenges in implementing an efficient data management system. Accordingly, we present details of the innovative database we developed, which allowed us to produce data that were protected against data entry errors and therefore more likely to be of high quality and reliability. Furthermore, it also provided us with evidence of protection. This database can also serve as a template for other epidemiological studies of NTDs in the future.Full Tex
UBC-Nepal Expedition: An experimental overview of the 2016 University of British Columbia Scientific Expedition to Nepal Himalaya
The University of British Columbia Nepal Expedition took place over several months in the fall of 2016 and was comprised of an international team of 37 researchers. This paper describes the objectives, study characteristics, organization and management of this expedition, and presents novel blood gas data during acclimatization in both lowlanders and Sherpa. An overview and framework for the forthcoming publications is provided. The expedition conducted 17 major studies with two principal goals—to identify physiological differences in: 1) acclimatization; and 2) responses to sustained high-altitude exposure between lowland natives and people of Tibetan descent. We performed observational cohort studies of human responses to progressive hypobaric hypoxia (during ascent), and to sustained exposure to 5050 m over 3 weeks comparing lowlander adults (n = 30) with Sherpa adults (n = 24). Sherpa were tested both with (n = 12) and without (n = 12) descent to Kathmandu. Data collected from lowlander children (n = 30) in Canada were compared with those collected from Sherpa children (n = 57; 3400–3900m). Studies were conducted in Canada (344m) and the following locations in Nepal: Kathmandu (1400m), Namche Bazaar (3440m), Kunde Hospital (3480m), Pheriche (4371m) and the Ev-K2-CNR Research Pyramid Laboratory (5050m). The core studies focused on the mechanisms of cerebral blood flow regulation, the role of iron in cardiopulmonary regulation, pulmonary pressures, intra-ocular pressures, cardiac function, neuromuscular fatigue and function, blood volume regulation, autonomic control, and micro and macro vascular function. A total of 335 study sessions were conducted over three weeks at 5050m. In addition to an overview of this expedition and arterial blood gas data from Sherpa, suggestions for scientists aiming to perform field-based altitude research are also presented. Together, these findings will contribute to our understanding of human acclimatization and adaptation to the stress of residence at high-altitude
Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis
Possible origins of macroscopic left-right asymmetry in organisms
I consider the microscopic mechanisms by which a particular left-right (L/R)
asymmetry is generated at the organism level from the microscopic handedness of
cytoskeletal molecules. In light of a fundamental symmetry principle, the
typical pattern-formation mechanisms of diffusion plus regulation cannot
implement the "right-hand rule"; at the microscopic level, the cell's
cytoskeleton of chiral filaments seems always to be involved, usually in
collective states driven by polymerization forces or molecular motors. It seems
particularly easy for handedness to emerge in a shear or rotation in the
background of an effectively two-dimensional system, such as the cell membrane
or a layer of cells, as this requires no pre-existing axis apart from the layer
normal. I detail a scenario involving actin/myosin layers in snails and in C.
elegans, and also one about the microtubule layer in plant cells. I also survey
the other examples that I am aware of, such as the emergence of handedness such
as the emergence of handedness in neurons, in eukaryote cell motility, and in
non-flagellated bacteria.Comment: 42 pages, 6 figures, resubmitted to J. Stat. Phys. special issue.
Major rewrite, rearranged sections/subsections, new Fig 3 + 6, new physics in
Sec 2.4 and 3.4.1, added Sec 5 and subsections of Sec
The Temperature, Electron, and Pressure Characteristics of Switchbacks: Parker Solar Probe Observations
Parker Solar Probe (PSP) observes unexpectedly prevalent switchbacks, which
are rapid magnetic field reversals that last from seconds to hours, in the
inner heliosphere, posing new challenges to understanding their nature, origin,
and evolution. In this work, we investigate the thermal states, electron pitch
angle distributions, and pressure signatures of both inside and outside
switchbacks, separating a switchback into spike, transition region (TR), and
quiet period (QP). Based on our analysis, we find that the proton temperature
anisotropies in TRs seem to show an intermediate state between spike and QP
plasmas. The proton temperatures are more enhanced in spike than in TR and QP,
but the alpha temperatures and alpha-to-proton temperature ratios show the
opposite trends, implying that the preferential heating mechanisms of protons
and alphas are competing in different regions of switchbacks. Moreover, our
results suggest that the electron integrated intensities are almost the same
across the switchbacks but the electron pitch angle distributions are more
isotropic inside than outside switchbacks, implying switchbacks are intact
structures but strong scattering of electrons happens inside switchbacks. In
addition, the examination of pressures reveals that the total pressures are
comparable through a switchback, confirming switchbacks are pressure-balanced
structures. These characteristics could further our understanding of ion
heating, electron scattering, and the structure of switchbacks.Comment: submitted to Ap
The Structure and Origin of Switchbacks: Parker Solar Probe Observations
Switchbacks are rapid magnetic field reversals that last from seconds to
hours. Current Parker Solar Probe (PSP) observations pose many open questions
in regards to the nature of switchbacks. For example, are they stable as they
propagate through the inner heliosphere, and how are they formed? In this work,
we aim to investigate the structure and origin of switchbacks. In order to
study the stability of switchbacks, we suppose the small scale current sheets
therein may work to braid and stabilize the switchbacks. Thus, we use the
partial variance of increments method to identify the small scale current
sheets, and then compare their distributions in switchbacks. With more than one
thousand switchbacks identified with PSP observations in seven encounters, we
find many more current sheets inside than outside switchbacks, indicating that
these micro-structures should work to stabilize the S-shape structures of
switchbacks. Additionally, with the helium measurements, we study the
variations of helium abundance ratios and alpha-proton differential speeds to
trace switchbacks to their origins. We find both helium-rich and helium-poor
populations in switchbacks, implying the switchbacks could originate from both
closed and open magnetic field regions in the Sun. Moreover, we observe that
the alpha-proton differential speeds also show complex variations as compared
to the local Alfv\'en speed. The joint distributions of both parameters show
that low helium abundance together with low differential speed is the dominant
state in switchbacks. The presence of small scale current sheets in switchbacks
along with the helium features are in line with the hypothesis that switchbacks
could originate from the Sun via interchange reconnection process. However,
other formation mechanisms are not excluded
Parker Solar Probe Observations of High Plasma Beta Solar Wind from Streamer Belt
In general, slow solar wind from the streamer belt forms a high plasma beta
equatorial plasma sheet around the heliospheric current sheet (HCS) crossing,
namely the heliospheric plasma sheet (HPS). Current Parker Solar Probe (PSP)
observations show that the HCS crossings near the Sun could be full or partial
current sheet crossing (PCS), and they share some common features but also have
different properties. In this work, using the PSP observations from encounters
4 to 10, we identify streamer belt solar wind from enhancements in plasma beta,
and we further use electron pitch angle distributions to separate it into HPS
solar wind that around the full HCS crossings and PCS solar wind that in the
vicinity of PCS crossings. Based on our analysis, we find that the PCS solar
wind has different characteristics as compared with HPS solar wind: a) PCS
solar wind could be non-pressure-balanced structures rather than magnetic
holes, and the total pressure enhancement mainly results from the less reduced
magnetic pressure; b) some of the PCS solar wind are mirror unstable; c) PCS
solar wind is dominated by very low helium abundance but varied alpha-proton
differential speed. We suggest the PCS solar wind could originate from coronal
loops deep inside the streamer belt, and it is pristine solar wind that still
actively interacts with ambient solar wind, thus it is valuable for further
investigations on the heating and acceleration of slow solar wind
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