Co-Production of Cellulase and Xylanase Enzymes By Thermophilic Bacillus subtilis 276NS

Co-production of thermostable extracellular cellulase and xylanase was investigated using bacterial soil isolate. To evaluate the effect of culture conditions on the c-oproduction of both enzymes by Bacillus subtilis 276NS (GenBank accession number JF801740), a Plackett-Burman fractional factorial design was applied. Among the tested variables, yeast extract, sucrose and incubation time were the most significant variables increased cellulase and xylanase productions. Both of xylan and CMC (Carboxymethyl Cellulose) induced the xylanse enzyme production. A near-optimum medium formulation was obtained which increased the cellulase and xylanase enzymes 5.7- and 1.08 –fold higher than the yield obtained with the basal medium, respectively. Thereafter, the response surface methodology was adopted to acquire the best process conditions among the selected variables (xylan, CMC and Yeast Extract (YE)) required for improving xylanase yield. The optimal combinations of the major medium constituents for xylanase production evaluated using non-linear optimization algorithm of EXCEL-solver, was as follows (g/L): D-sucrose, 10; xylan, 10.367, CMC, 10.535; (NH4)2SO4, 1.0; YE, 1.71; Tween-80, 0.4 and FeSO4, 0.25 mg/L, at pH 8.0, temperature 35◦C and incubation time 24h under shaking. The predicted optimum thermostable xylanase activity was 360 U/ml, which was around 4-times the activity with the basal medium

UTILIZING WASTE HEAT FROM THE REFRIGERATION CYCLE BY USING A TWO-STAGE HEAT EXCHANGER

Refrigeration, and air conditioning systems are designed to transport heat from internal spaces or products and discard it into the surrounding. Refutation of heat might happen in a straight line to the air, as in the case of most conventional units of air-source, or to water flowing from a cooling tower. This heat is of a "low-grade diversity", it still signifies wasted energy. According to the viewpoint of energy conservation, it would be necessary to regain this heat in a serviceable form. In this research, a practical study aims to collect the heat emitted from the cooling condenser by 35% and exploit it in heating hot water for domestic purposes, in addition to other uses, including reducing the consumption of electrical energy as a result of exploiting and collecting waste heat, from cooling devices using a system consisting of two types of helical heat exchangers and two stages. The first stage consists of a tube-in-tube helical exchanger and the second stage includes a tube-in-shell heat exchanger using R410A refrigerant. The EES engineering equation solving program was used to solve the numerical equations that are used to solve problems related to thermodynamics, due to its high accuracy in solving equations, extracting values and graphs, and calculating the performance coefficient of the COP to obtain better efficiency for the work of the system, as it was found that the higher the temperature. As a result of increasing the flow rate of deionized water, the percentage of performance coefficient of COP has increased

Syntheses, Structures and Biological Activity of Some Schiff Base Metal Complexes

اربع معقدات قاعدة شف ثنائية النواة جديدة [(MCl2)2L] = M} Fe (1), (2) Co, Cu (3), Sn (4) { ليكاند = 1,4-ثنائي فنيلين (ميثان دايين) ثنائي(ايثان ثنائي امين). حضرت بطريقة التفاعل المباشر بين اليكاند وعدد من هاليدات الفلزات. الصيغ التركيبية للمركبات اثبتت من خلال عدد من التقنيات الطيفية مكيف الاشعة تحت الحمراء وطيف الرنين النووي المغناطيسي وطيف الكتلة. حددت الفعالية الحيوية للمركبات تجاه عدد من العزلات البكتيرية السالبة والموجبة الغرام مثل العصية القولونية, الزوائف, العصيات, المكورات العنقودية, المسبحات العنقودية.Four new binuclear Schiff base metal complexes [(MCl2)2L] {M = Fe 1, Co 2, Cu 3, Sn 4, L = N,N’-1,4-Phenylenebis (methanylylidene) bis (ethane-1,2-diamine)} have been synthesized using direct reaction between proligand (L) and the corresponding metal chloride (FeCl2, CoCl2, CuCl2 and SnCl2). The structures of the complexes have been conclusively determined by a set of spectroscopic techniques (FT-IR, 1H-NMR, and mass spectra). Finally, the biological properties of the complexes have been investigated with a comparative approach against different species of bacteria (E. coli G-, Pseudomonas G-, Bacillus G+, Staphylococcus G+, and Streptococcus G+)

Dynamic reciprocal authentication protocol for mobile cloud computing

A combination of mobile and cloud computing delivers many advantages such as mobility, resources, and accessibility through seamless data transmission via the Internet anywhere at any time. However, data transmission through vulnerable channels poses security threats such as man-in-the-middle, playback, impersonation, and asynchronization attacks. To address these threats, we define an explicit security model that can precisely measure the practical capabilities of an adversary. A systematic methodology consisting of 16 evaluation criteria is used for comparative evaluation, thereby leading other approaches to be evaluated through a common scale. Finally, we propose a dynamic reciprocal authentication protocol to secure data transmission in mobile cloud computing (MCC). In particular, our proposed protocol develops a secure reciprocal authentication method, which is free of Diffie–Hellman limitations, and has immunity against basic or sophisticated known attacks. The protocol utilizes multifactor authentication of usernames, passwords, and a one-time password (OTP). The OTP is automatically generated and regularly updated for every connection. The proposed protocol is implemented and tested using Java to demonstrate its efficiency in authenticating communications and securing data transmitted in the MCC environment. Results of the evaluation process indicate that compared with the existing works, the proposed protocol possesses obvious capabilities in security and in communication and computation costs

Incidence and contributing factors of glucose intolerance in Saudi postpartum women: Sub-group analysis from RAHMA study

Objectives: The objectives of this study were to determine incidence and risk factors of glucose intolerance one year after delivery in a sub-cohort of Riyadh Mother and Baby Cohort Study (RAHMA) study. Methods: This is a follow-up study of a sub-cohort from RAHMA study from King Khalid University Hospital (KKUH). All women from RAHMA database who completed one year since delivery at KKUH were contacted by phone to participate in the study. Previously collected data from RAHMA registry for each participant were linked to this study data. Clinical data measured for each participant included current weight and height to calculate the BMI and waist circumference. Blood tests done for each participant were fasting blood glucose (FPG) and HbA1c. Based on the blood tests results, participants were classified into three groups; diabetic, pre-diabetic and normal. The incidence of diabetes and prediabetes was calculated for the whole cohort. Clinical, biochemical, and sociodemographic predictors of glucose intolerance were compared between the three groups. Risk factors with P-value less than 0.05 were tested in multivariate regression model with bootstrapping to calculate the relative risk (RR) and its 95% Bias corrected Confidence Interval (C.I.) Results: From the sub-cohort, 407 women fulfilled the inclusion criteria and agreed to participate in the study. From the study participants; 250 (61.4%) women were normoglycemic, 142 (35%) women had prediabetes and 15 (3.6%) women were diabetic. Following multivariable regression analysis only history of gestational diabetes mellitus (GDM), (RR 1.74, 95% CI (1.06 to 2.84), P = 0.01), obesity (RR 1.69, 95% CI (1.01–3.11), P = 0.04) and diastolic blood pressure, (RR 1.04, 95% CI (1.01–1.09), P = 0.03) remained as predictors of postpartum glucose intolerance. Conclusion: The incidence of postpartum glucose intolerance (diabetes and prediabetes) is very high in Saudi women. Both GDM and obesity are strong predictors of glucose intolerance

Characterization of an isolated lactase enzyme produced by Bacillus licheniformis ALSZ2 as a potential pharmaceutical supplement for lactose intolerance

IntroductionLactose intolerance is a widespread problem that affects people of many different races all over the world. The following pharmacological supplements can improve the lives of those who suffer from this issue.MethodsThis work focused on lactase producer isolation and statistical design (Plackett–Burman, and BOX–Behnken) to maximize the effectiveness of environmental factors. A lactase-producing bacterium was chosen from a discovery of 100 strains in soil that had previously been polluted with dairy products. Plackett-Burman investigated fifteen variables.ResultsThe most critical variables that lead to increased lactase synthesis are glucose, peptone, and magnesium sulfate (MgSO4). The ideal process conditions for the creation of lactase yield among the stated variables were then determined using a BOX-Benken design. To establish a polynomial quadratic relationship between the three variables and lactase activity, the Box–Behnken design level was used. The EXCEL-solver nonlinear optimization technique was used to predict the best form for lactase production. The ideal temperature and pH levels have been determined, both before and after the lactase purification process, to achieve the highest performance of isolated lactase.ConclusionAccording to this study, Bacillus licheniformis is a perfect supply of the lactase enzyme (β -Galactosidase), It can be used as a product to assist people who have health issues due to lactose intolerance

Prevalence of intestinal helminthes in feral cats in Babylon province/ Iraq, urban and rural locations

This study carried on fifty seven feral cats in Babylon province urban and rural locations. The cats euthanized and examined for gastrointestinal helminthes (GI). The results revealed that all examined cats were infected by one or more GI helminthes, which were including Toxocara cati, Toxascaris leonina, Ancylostoma tubaeforme, Pterygodermatites cahirensis, Dipylidium caninum, Dipylidium nölleri, Taenia taeniaeformis and Joyeuxiella pasqualei. The highest prevalence nematode occurred in urban habitant cats was Toxocara cati (43.75%) and the lowest was Pterygodermatites cahirensis (12.5%). However, in rural habitant cat, the highest prevalence rate of nematode was Physaloptera praeputialis and Toxocara cati (24%) followed by Ancylostoma tubaeforme (16%) and Pterygodermatites cahirensis (4 %). Among cestodes helminthes, the most prevalence species in urban habitant cat was Taenia taeniaeformis (31.25%) followed by Diplopylidium nölleri (25%), Dipylidium caninum (21.88%) and Joyeuxiella pasqualei (12.5 %.). In rural habitant cats, the most prevalent cestode was Diplopylidium nölleri (52%), followed by Diplopylidium caninum (36%), Taenia taeniaeformis (32%) and Joyeuxiella pasqualei (20%)

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications