Integrated potassium management through composted straws and inorganic fertilizer in maize

Using organic and inorganic fertilizers concurrently may not only quench the crop nutritive demands but also been proved an eco-friendly nutrient source. In order to evaluate the effect of potassium (K) application from only inor- ganic, integrated organic and inorganic sources of fertilizers on maize, a field study was conducted during 2008 and 2009 following randomized complete block design (RCBD) with three replications at Agronomic Research Area, University of Agriculture, Faisalabad , Pakistan. In this study composted wheat and rice straws were inte- grated with the sulphate of potash (SOP) as K source. Regarding application of K from the integrated sources, treatment containing 25% composted rice straw + 75% SOP provided highest leaf area index (LAI; 5.13 and 5.11), eaf area duration (LAD; 217.33 and 219.65 days), crop growth rate (CGR; 28.94 and 29.08 g m-2 d-1), total dry matter (TDM; 1,925.03 and1,931.32 g m-2), and gave prominent plant height (215.50 and 218.63 cm), 1,000-grain weight (262.87 and 266.63 g), grain yield (7.02 and 7.38 t ha-1), biological yield (16.63 and 17.37 t ha-1) and harvest index (42.22 and 42.49%) during both the years (2008-09), respectively. Moreover, given years, the integrated use of 25% composted rice straw + 75% SOP earned maximum benefit cost ratio of 2.12 and Rs. 2.22, respectively. Conclusively, integrated use of organic amendments of K with inorganic source might be a better technique over separate application of inorganic source

Comparative Expression Studies of Fiber Related Genes in Cotton Spp.

Cotton fibers are the seed trichomes that are developed around the seed and are used to make clothes and yarn for the textile industry. Expression profiling of cotton fiber genes is very important to estimate the differential gene expression level at different fiber developmental stages. Expression analysis of fiber developing genes are very important to enhance the fiber length of cotton. The expression profiling of three gene families in five stages (0, 5, 10, 15 and 20 DPA) of cotton fiber tissues was carried out through real-time PCR. Expression analysis revealed that transcripts of GA-20 Oxidase, XTH, and PEPc were elevated from 5 to 20 days post-anthesis (DPA) fibers. Total RNA was extracted from various stages of cotton fiber development and was reverse transcribed to cDNA for PCR amplification. For data normalization, 18s rRNA was used as an internal control. The objective of this study was to explore the expression level of fiber developing genes at specific stages of fiber development. The results showed that most of the genes were expressed during the elongation phase in between 5 DPA to 15 DPA. Results obtained from this study may be helpful for the further identification of fiber genes and the improvement of fiber characteristics in cotton. PEPc and XTH genes that are expressed with a high rate during the fiber development may be used in breeding programmes for the improvement of fiber quality and quantity

Comparative Expression Studies of Fiber Related Genes in Cotton Spp.

Cotton fibers are the seed trichomes that are developed around the seed and are used to make clothes and yarn for the textile industry. Expression profiling of cotton fiber genes is very important to estimate the differential gene expression level at different fiber developmental stages. Expression analysis of fiber developing genes are very important to enhance the fiber length of cotton. The expression profiling of three gene families in five stages (0, 5, 10, 15 and 20 DPA) of cotton fiber tissues was carried out through real-time PCR. Expression analysis revealed that transcripts of GA-20 Oxidase, XTH, and PEPc were elevated from 5 to 20 days post-anthesis (DPA) fibers. Total RNA was extracted from various stages of cotton fiber development and was reverse transcribed to cDNA for PCR amplification. For data normalization, 18s rRNA was used as an internal control. The objective of this study was to explore the expression level of fiber developing genes at specific stages of fiber development. The results showed that most of the genes were expressed during the elongation phase in between 5 DPA to 15 DPA. Results obtained from this study may be helpful for the further identification of fiber genes and the improvement of fiber characteristics in cotton. PEPc and XTH genes that are expressed with a high rate during the fiber development may be used in breeding programmes for the improvement of fiber quality and quantity

Consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.)

A two year field experiment was conducted to check the consequences of diverse use of nitrogen sources on grain yield, grain quality and growth attributes of hybrid maize (Zea mays L.) at the Agronomic Research Area, University of Agriculture, Faisalabad during Autumn 2008 and 2009. Experiments were laid out in a randomized complete block design with factorial arrangement comprising 3 replications with a net plot size of 3 × 5 m. Treatment comprised two hybrids: that is, H1 (Pioneer-30Y87) and H2 (Pioneer-31R88) with combination of six nitrogen sources S0 : Control (0) kg N ha-1, S1: Urea (50%) + Poultry manure (50%), S2: Urea (50%) + Farm Yard Manure (50%) , S3: Urea (50%) + Pressmud of sugarcane manure (50%), S4: Urea (50%) + Compost (50%), S5: Urea (50%) + (PM+FYM+PMS+ Compost) 50% . Results of grain yield (t ha-1), grain protein content (%) grain oil content (%), leaf area index, leaf area duration, dry matter accumulation, crop growth rate and net assimilation rate was found to be significant during 2008 and 2009. It was concluded that hybrid maize H1 (Pioneer - 30Y87) produced better grain yield (6.14 t ha-1) during 2008 when nitrogen sources S1: Urea (50%) + Poultry manure (50%) was applied in combination as compared to grain yield (6.0 t ha-1) in hybrid H2 (Pioneer -31R88) during 2009. Growth and quality attributes also performed better in 2008 as compared to 2009 at nitrogen sources S1: Urea (50%) + Poultry manure (50%).Key words: Nitrogen sources, hybrid maize, yield, growth, quality

Mulberry based zinc nano-particles mitigate salinity induced toxic effects and improve the grain yield and zinc bio-fortification of wheat by improving antioxidant activities, photosynthetic performance, and accumulation of osmolytes and hormones

Salinity stress (SS) is a challenging abiotic stress that limits crop growth and productivity. Sustainable and cost effective methods are needed to improve crop production and decrease the deleterious impacts of SS. Zinc (Zn) nanoparticles (NPs) have emerged as an important approach to regulating plant tolerance against SS. However, the mechanisms of SS tolerance mediated by Zn-NPs are not fully explained. Thus, this study was performed to explore the role of Zn-NPs (seed priming and foliar spray) in reducing the deleterious impacts of SS on wheat plants. The study comprised different SS levels: control, 6 and 12 dS m−1, and different Zn-NPs treatments: control, seed priming (40 ppm), foliar spray (20 ppm), and their combination. Salinity stress markedly reduced plant growth, biomass, and grain yield. This was associated with enhanced electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2), sodium (Na), chloride (Cl) accumulation, reduced photosynthetic pigments, relative water contents (RWC), photosyntetic rate (Pn), transpiration rate (Tr), stomata conductance (Gs), water use efficiency (WUE), free amino acids (FAA), total soluble protein (TSP), indole acetic acid (IAA), gibberellic acid (GA), and nutrients (Ca, Mg, K, N, and P). However, the application of Zn-NPs significantly improved the yield of the wheat crop, which was associated with reduced abscisic acid (ABA), MDA, H2O2 concentration, and EL, owing to improved antioxidant activities, and an increase in RWC, Pn, Tr, WUE, and the accumulation of osmoregulating compounds (proline, soluble sugars, TSP, and FAA) and hormones (GA and IAA). Furthermore, Zn-NPs contrasted the salinity-induced uptake of toxic ions (Na and Cl) and increased the uptake of Ca, K, Mg, N, and P. Additionally, Zn-NPs application substantially increased the wheat grain Zn bio-fortification. Our results support previous findings on the role of Zn-NPs in wheat growth, yield, and grain Zn bio-fortification, demonstrating that beneficial effects are obtained under normal as well as adverse conditions, thanks to improved physiological activity and the accumulation of useful compounds. This sets the premise for general use of Zn-NPs in wheat, to which aim more experimental evidence is intensively being sought. Further studies are needed at the genomic, transcriptomic, proteomic, and metabolomic level to better acknowledge the mechanisms of general physiological enhancement observed with Zn-NPs application

Synchronization of Boron application methods and rates is environmentally friendly approach to improve quality attributes of Mangifera indica L. on sustainable basis

Micronutrient deficiency in the soil is one of the major causes of mango fruit and yield's poor quality. Besides, the consumption of such a diet also causes a deficiency of micronutrients in humans. Boron deficiency adversely affects the flowering and pollen tube formation, thus decreasing mango yield and quality attributes. Soil and foliar application of B are considered a productive method to alleviate boron deficiency. A field experiment was conducted to explore the Boron most suitable method and application rate in mango under the current climatic scenario. There were nine treatments applied in three replications. The results showed that application of T8 = RD + Borax (75 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) and T9 = RD + Borax (150 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) significantly enhanced the nitrogen, potassium, proteins, ash, fats, fiber, and total soluble solids in mango as compared to the control. A significant decrease in sodium, total phenolics contents, antioxidant activity, and acidity as citric acid also validated the effective functioning of T8 = RD + Borax (75 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) and T9 = RD + Borax (150 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) as compared to control. In conclusion, T8 = RD + Borax (75 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) and T9 = RD + Borax (150 g plant -1 as a basal application) + H3 BO3 (0.8% as a foliar spray) is a potent strategy to improve the quality attributes of mango under the changing climatic situation

INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY Continuing Education Article Genetic Resources and Diversity in Pakistani Goats

ABSTRACT Goats are the fastest growing ruminants in Pakistan. Preference for goat meat is the major reason for its increased demand. There are 25 goat breeds in the country and two wild relatives such as Markhor and Ibex. Estimated goat population in the country in 2006 is 53.8 millions. Documentation of goat breeds have depended mostly on the annual reports of various research institutes involved in goat production. Meat is the primary breeding objective and milk gets the secondary importance. Appreciable diversity among and within goat breeds exists for morphological, growth, fertility and other traits. Adult body weight, for example, may vary from 20-200 kg. Crossbreeding at farmer level continues to some extent. Organized crossbreeding efforts include crossing of Hairy and imported Angora for the production of mohair. However, crossbreds could not achieve status of commercial animals and are being kept as pets. Any new attempt for crossbreeding should thus be recommended in the light of previous attempts, because short term breeding objectives and limited technical and financial resources could result in failures only. Within-breed selection is a preferred strategy for improvement of traits of economic importance but this requires capacity building of various stakeholders. Indigenous goat resources can be better utilized on sustainable basis if efforts of research and development address indigenous problems. Breed documentation both at phenotypic and genetic level is a priority research area

GENETIC RESOURCES AND DIVERSITY IN DAIRY BUFFALOES OF PAKISTAN

Buffalo is the main dairy animal in Pakistan. There are five known buffalo breeds in the country namely: Nili, Ravi, Nili-Ravi, Kundhi and Azi Kheli (or Azakhale). Population trend is available for Nili-Ravi and Kundhi breeds and is positive. Azi-Kheli breed was included in 2006 livestock census for the first time. General production system is low-input extensive system but high input intensive system prevails around most cities in the form of buffalo colonies for supplying fresh milk. Buffaloes are seasonal breeders. Vast diversity exits both at phenotypic and genetic level. Economic traits have a wide variation and genetic control is moderate for production traits but very low for reproduction traits. Inbreeding is inimical to genetic diversity and has been reported to deteriorate productivity. Efforts to improve productivity of the species are needed alongwith sustainable utilization of existing resources

Current Understandings on Magnesium Deficiency and Future Outlooks for Sustainable Agriculture

The productivity of agricultural produce is fairly dependent on the availability of nutrients and efficient use. Magnesium (Mg2+) is an essential macronutrient of living cells and is the second most prevalent free divalent cation in plants. Mg2+ plays a role in several physiological processes that support plant growth and development. However, it has been largely forgotten in fertilization management strategies to increase crop production, which leads to severe reductions in plant growth and yield. In this review, we discuss how the Mg2+ shortage induces several responses in plants at different levels: morphological, physiological, biochemical and molecular. Additionally, the Mg2+ uptake and transport mechanisms in different cellular organelles and the role of Mg2+ transporters in regulating Mg2+ homeostasis are also discussed. Overall, in this review, we critically summarize the available information about the responses of Mg deficiency on plant growth and development, which would facilitate plant scientists to create Mg2+-deficiency-resilient crops through agronomic and genetic biofortification

Integration of organic sources with inorganic phosphorus increases hybrid maize performance and grain quality

Integrative use of organic and inorganic fertilizers not only fulfills the nutritional needs of crops, but also ensures sustainable production and environmental protection. Thus, this study was conducted to determine the influence of phosphorous application from integrated organic and inorganic fertilizers on maize. In this study poultry manure and press mud were integrated with single superphosphate (SSP) as the phosphorus source. Maximum amounts of time to 50% tasseling (47.75 and 47.63 days), 50% silking (53.07 and 53. 11 days) and maturity (106.47 and 106.49 days) were recorded where the recommended dose of phosphorus was applied from only inorganic sources. For application of phosphorus from integrated sources, the treatment containing 25% poultry manure + 75% SSP provided the highest number of grains per cob (459.60 and 460.70), 100-grain weight (26.85 and 27.82), stover yield (9.61 and 10.41 t ha-1), grain yield (7.14 and 7.82 t ha-1) and grain protein content (8.59 and 8.63%) during both years. However, all treatments had non-significant effects on grain oil and starch content. Moreover, in given years, the integrated use of 75% poultry manure and 25% SSP resulted in a maximum benefit-cost ratio of 2.97 and 3.11 USD, respectively, as compared to integrated use of 25% poultry manure and 75% SSP, which produced a benefit-cost ratio of 2.58 and 2.81 USD. In conclusion, integration of organic sources with inorganic phosphorus substantially improves the yield and economics of hybrid maize