Comparison of two hormone preparations on the reproductive performance of air breathing catfish Clarias batrachus (Lin.)

Two hormone preparations viz. Human Chorionic Gonadotropin (HCG) and pituitary gland (PG) suspension were compared for their comparative efficacy on the breeding performance of a air breathing catfish Clarias batrachus. It was found that HCG induced fish gave better ovulation response than PG. Both fertilization and hatching of eggs were significantly (p<O.Ol) higher in HCG treated fish than PG. On all consideration, HCG was found more suitable for induced breeding of C batrachus over PG

Optimum dietary carbohydrate to lipid ratio in stinging catfish, Heteropneustes fossilis (Bloch, 1792)

A feeding trial of 8 weeks was conducted in a static indoor rearing system to investigate the optimum carbohydrate to lipid ratio (CHO:L ratio) in stinging catfish, Heteropneustes fossilis. Five iso-nitrogenous (35% crude protein) and iso-energetic (17.06 kJ gˉ¹ gross energy (GE)) fish meal based diets with varying carbohydrate to lipid (CHO:L g/g) ratios of 0.60, 0.98, 1.53, 2.29 and 3.44 for diets 1-5, were tested, respectively. The diets containing a fixed protein to energy ratio (P:E ratio) of 20.50-mg protein kJˉ¹ GE were fed to triplicate groups of 40 fish (per 70-L tank). Fish were fed 5% of their body weight per day adjusted fortnightly. Diet 1, containing 10% carbohydrate and 17% lipids with a CHO:L ratio of 0.60 produced the poorest (p<0.05) growth rates, feed and protein efficiency. Increasing carbohydrate content in the diets to 26% concomitant with a reduction in lipid content to 11% with a CHO:L ration of 2.29 of diet 5 significantly improved (p<0.05) growth rates, feed and protein efficiency. But did not differ with diet 4, containing CHO:L ratio 2.29. A further increase in dietary carbohydrate up to 31% and a decrease in lipids levels to 9% with a CHO:L ratio ranging from 2.29 to 3.44 (diet 4-5) did not significantly improve the fish performance. Apparent net protein utilisation (ANPU) of fish fed diet 5 was higher (p<0.05) than for diets 1 and 2 but did not differ from diets 3 and 4. Higher lipid deposition (p<0.05) in whole body was observed with decreasing dietary CHO:L ratios as increasing lipid levels. Whole body protein of fish fed varying CHO:L diets did not show any discernible changes among the dietary treatments. This study revealed that H. fossilis can perform equally well on diets containing carbohydrate ranging from 26 to 31%, with 9 to 11% lipid or at CHO:L g/g ratio of 2.29-3.44

Therapeutic effects of Laser and L-carnitine against amiodarone-induced pulmonary toxicity in adult male rats

Purpose: To compare the effects of laser and/or L-carnitine (LC) on amiodarone (AM)-induced lung toxicity in adult male rats.Methods: Lung toxicity was induced in 50 healthy male albino rats (150-180 g) by AM for 8 weeks. The rats were divided into 4 groups (7 per group): AM recovery, LC, laser, and laser + LC. After a 4-week exposure to laser and/or LC, key antioxidant enzymes, nitric oxide (NO), angiotensin II, cyclooxygenase-II (COX-II) and lipoxygenase (LOX) as well as oxidative stress and inflammation parameters were determined.Results: Administration of LC and/or laser exposure led to significant increases in reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and angiotensin II; and significant decreases in nitric oxide (NO) level and glutathione-S-transferase (GST) activity in the AM-treated rats when compared with the AM recovery group (p ˂ 0.05). The LC and/or laser exposure also significantly inhibited COX II and LOX activities in the lungs, and brought about significant reduction in levels of TNF-α and lipid peroxidation (LPO), and significant increases in cytochrome levels (p ˂ 0.05).Conclusion: These results indicate that AM induced pulmonary fibrosis in rats. However, AM withdrawal and treatment by LC and/or exposure to laser exerted mitigating effects against the AMinduced fibrosis.Keywords: Amiodarone, Lung toxicity, Laser; L-carnitin

Introduction of emergency contraceptive pills in the public health system of Pakistan: A south-to-south collaboration

This report describes a south-to-south collaboration to assist the Ministry of Health of Pakistan to introduce emergency contraception (EC) into its family planning program, supported by the Population Council’s USAID-funded Frontiers in Reproductive Health (FRONTIERS) program. The collaboration consisted of a three-day visit by senior managers from the Pakistani health authorities to Bangladesh to familiarize them with the EC program in that country, followed by a two-day consultative meeting in Islamabad to draw up concrete plans for introduction of the method into the Pakistan system. Participants at the consultation developed plans to introduce EC into both community-based distribution and clinical programs. The plans included staff training; commodity logistics; and development of information, education, and communication materials, many of which will be adapted from materials used in other EC projects in the region supported by FRONTIERS. The activity successfully met its goals. Providing south-to-south technical assistance was demonstrated to be an effective strategy for introducing sensitive services and is recommended for other regions and similar services

Effect of Aqueous Leaf Extract of Jatropha tanjorensis on parasitaemia and haematological parameters in mice infected with Plasmodium ber ghei

Medicinal plants are proven sources of many useful drugs in our modern world. Jatropha tanjorensis, known as hospital too far, is consumed highly in Africa as herbal medicine. This research work was done to evaluate the effect of aqueous leaf extract of Jatropha tanjorensis on parasitaemia and haematological parameters in mice infected with Plasmodium ber ghei. The leaves of J. tanjorensis were extracted with distilled water and qualitative phytochemical analysis carried out. Acute toxicity studies were carried out using the Organization for Economic Cooperation and Development (OECD) guideline. The curative activity of the extract was examined using Rane’s test. A total of forty (40) mice were used for the study and they were infected with Plasmodium berghei. The infected mice were subdivided into five groups of six mice each and treated with different doses of standard drug (artemether/lumefantrine 25mg/kg) and extract (200, 400 and 800mg/kg body weight) for 4 days. After treatment, blood was collected and used for percentage parasitaemia, packed cell volume and hemoglobin concentration. The result of phytochemical analysis revealed the presence of alkaloids, tannins, saponins, flavonoids, terpe noids, cardiac glycosides and anthra quinones. The result of the acute toxicity showed the signs of neither neurological, behavioral nor mortality at concentrations of 2,000 and 5,000 mg/kg oral doses within the first 24 hours and during the 14 days study period. A dose- dependent increase was observed in Total haemoglobin (Hb) and Packed cell volume (PCV) levels which was collaborated with increase in weight as compared with negative control. The leaf extract of J. tanjorensis also revealed a significant (p&lt;0.05) suppression activity and mean survival time at the doses of 400 mg/kg, 200 mg/kg and 100 mg/kg when compared with the negative control. The extract of Jatropha tanjorensis showed reasonable levels of anti-anaemia and antimalarial activities with no signs of acute toxicity.&nbsp; Therefore this study may support its use as an anti-prophylactic and blood tonic nutraceutical

Implications of the Top Quark Mass Measurement for the CKM Parameters, xsx_s and CP Asymmetries

Motivated by the recent determination of the top quark mass by the CDF collaboration, \mt =174 \pm 10 ^{+13}_{-12} GeV, we review and update the constraints on the parameters of the quark flavour mixing matrix $V_{CKM}$ in the standard model. In performing our fits, we use inputs from the measurements of the following quantities: (i) \abseps, the CP-violating parameter in $K$ decays, (ii) \delmd, the mass difference due to the \bdbdbar\ mixing, (iii) the matrix elements \absvcb and \absvub, and (iv) $B$-hadron lifetimes. We find that the allowed region of the unitarity triangle is very large, mostly due to theoretical uncertainties. (This emphasizes the importance of measurements of CP-violating rate asymmetries in the $B$ system.) Nevertheless, the present data do somewhat restrict the allowed values of the coupling constant product $f_{B_d}\sqrt{\hat{B}_{B_d}}$ and the renormalization-scale invariant bag constant $\hat{B}_K$. With the updated CKM matrix we present the currently-allowed range of the ratio $\vert V_{td}/V_{ts} \vert$, as well as the standard model predictions for the \bsbsbar\ mixing parameter \xs and the quantities $\sin 2\alpha$, $\sin 2\beta$ and $\sin^2\gamma$, which characterize the CP-asymmetries in $B$-decays. The ALEPH collaboration has recently reported a significant improvement on the lower limit on the \bs-\bsb mass difference, $\Delta M_s/\Delta M_d > 11.3$ (95\% C.L.). This has interesting consequences for the CKM parameters which are also worked out. NOTE: this is a revised and updated version of our previous paper.Comment: LaTeX, 27 pages, 16 uuencoded figures (enclosed), CERN-TH.7398/94, UdeM-GPP-TH-94-0

Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis.

Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilaparvata lugens, one from rice and the other from Leersia hexandra (Poaceae), a wetland forage grass, and we also investigated whether host plant DNA contaminates that of herbivore insects in extractions of whole insects. Ingestion and assimilation of food were reduced significantly when individuals derived from one host plant were caged on the other species. The bands, OPA3 (1.25), OPD3 (1.10), OPD3 (0.80), OPD3 (0.60), pUC/M13F (0.35), pUC/M13F (0.20), BOXAIR (0.50), peh#3 (0.50), and peh#3 (0.17) were found in both rice-infesting populations of brown planthopper and its host plant (rice). Similarly, the bands, OPA4 (1.00), OPB10 (0.70), OPD3 (0.90), OPD3 (0.80), OPD3 (0.60), pUC/ M13F (0.35), pUC/M13F (0.20), and BOXAIR (0.50) were found in both Leersia-infesting populations of brown planthopper and the host plant. So, it is clear that the DNA bands amplified in the host plants were also found in the extracts from the insects feeding on them

Strip planting decreases nitrogen fertilizer requirements while retention of more residue increases them in a rice - wheat - mungbean sequence on a subtropical floodplain soil

Conservation agriculture (CA) has not been well developed for intensively cultivated (2-3 crops yr-1) rice- based cropping systems which produce large amounts of crop residues annually. Thus, we examined the effects of two crop establishment systems (minimum soil disturbance by strip planting (SP) or conventional tillage (CT)), two residue retention levels (low and high) and five N rates (60, 80, 100, 120 & 140% of the recommended N fertilizer doses (RFD) on nine consecutive crops on an Aeric Haplaquept under rice-wheat- mungbean sequence. Rice yields were comparable between the crop establishment types but system yields were significantly higher with SP in two out of three years compare to CT. Increased residue retention did not significantly influence rice yield but positively influenced system yields. No substantial differences in optimum N rate was estimated between CT and SP for 90% of maximum yield goal (MYG) for all the three years but substantially decreased in SP compared to CT in two out of three years for 95 and 99% of MYG. The N fertilizer requirement was 6-22% higher with high residue retention compared to low residue retention plots for all the three yield goal levels. High residue retention also increased soil organic carbon (SOC) at 0- 6 cm depth in both tillage treatments. In conclusion, introducing CA did not alter the N fertilizer requirements of rice for 90% of MYG but reduced the requirement for 95 and 99% of MYG compared to CT. However, there was evidence that the retained crop residue immobilized N and increased the fertilizer N requirement

QCD Corrections and the Endpoint of the Lepton Spectrum in Semileptonic B Decays

Recently, Neubert has suggested that a certain class of nonperturbative corrections dominates the shape of the electron spectrum in the endpoint region of semileptonic $B$ decay. Perturbative QCD corrections are important in the endpoint region. We study the effects of these corrections on Neubert's proposal. The connection between the endpoint of the electron spectrum in semileptonic $B$ decay and the photon spectrum in $b\rightarrow s\gamma$ is outlined.Comment: 18 pages, uses REVTeX, UCSD/PTH 93-38, CALT-68-1910, JHU-TIPAC-930029 (some changes to the discussion of subleading radiative corrections, and minor typos fixed