Hsp90 Oligomers Interacting with the Aha1 Cochaperone: An Outlook for the Hsp90 Chaperone Machineries

The 90-kDa heat shock protein (Hsp90) is a highly flexible dimer able to self-associate in the presence of divalent cations or under heat shock. This study investigated the relationship between Hsp90 oligomers and the Hsp90 cochaperone Aha1 (activator of Hsp90 ATPase). The interactions of Aha1 with Hsp90 dimers and oligomers were evaluated by ultracentrifugation, size-exclusion chromatography coupled to multiangle laser light scattering and high-mass matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Hsp90 dimer was able to bind up to four Aha1 molecules, and Hsp90 oligomers are also able to interact with Aha1. The binding of Aha1 did not interfere with the Hsp90 oligomerization process. Except for Hsp90 dimer, the stoichiometry of the interaction remained constant, at 2 Aha1 molecules per Hsp90 dimer, regardless of the degree of Hsp90 oligomerization. Moreover, Aha1 predominantly bound to Hsp90 oligomers. Thus, the ability of Hsp90 oligomers to bind the Aha1 ATPase activator reinforces their role within the Hsp90 chaperone machineries

IN/LEDGF/INI1-IBD/DNA structure.

<p><b>A</b>. INI1-IBD (pink) sits on the top of the complex and interacts with the C and N termini of the two IN monomers B. An extension of INI1-IBD interacts with the C-terminus of the two monomers A. <b>B</b>. A 90° view sliced as shown by the dotted square in figure A. <b>C and D</b>: two 90° views showing clearly the IN interacting domains with INI1.</p

Structure-function analysis.

<p><b>A</b>. IN/LEDGF/vDNA structure in the 3′ processing conformation. <b>B</b>. In the IN/LEDGF/INI1-IBD/vDNA complex, INI1-IBD caps the surface of the complex and blocks IN in an intermediate conformation, as shown by the position of the viral DNA (yellow). <b>C.</b> IN/LEDGF/vDNA/tDNA structure in the strand transfer conformation after release of INI1 and binding to the target DNA (red).</p

Cryo-EM structure.

<p><b>A</b>. Negatively stained structures of the IN/LEDGF (blue) and IN/LEDGF/INI1-IBD (gold) complexes. The difference between the two maps representing the position of INI1-IBD is in red. <b>B</b>. Cryo-EM structure of the IN/LEDGF/INI1-IBD/DNA complex represented by three perpendicular views. <b>C</b>. Difference map calculated by subtracting the density corresponding to the fitted atomic models of IN and LEDGF from the IN/LEDGF/INI1-IBD/DNA cryo-EM map. The differences, corresponding respectively to INI1-IBD, DNA and LEDGF, are represented in violet, yellow and grey. <b>D</b>. Three perpendicular views of the IN/LEDGF/INI1-IBD/DNA complex: INI1-IBD is in violet, LEDGF in grey and viral DNA in yellow. The fitted IN model is represented as molecular surface. For both IN dimers, the two monomers are in blue and gold. The two IN dimers (light and dark) are related by a twofold symmetry.</p

Superimposition of the IN/LEDGF/DNA and IN/LEDGF/INI1-IBD/DNA structures.

<p><b>A:</b> Superimposition of the IN/LEDGF/DNA (grey) and IN/LEDGF/INI1-IBD/DNA (blue) structures. <b>B:</b> DNA fitting. The spikes in the two structures unambigously revealed the DNA position. The DNA (yellow) is fitted in the IN/LEDGF/INI1-IBD/DNA map (mesh representation) with a 40° rotation from its position in the IN/LEDGF/DNA map (surface representation).</p

Topology of the IN tetramer.

<p><b>A:</b> View from the INI1-IBD side of the complex. <b>B:</b> View of the IN tetramer after removing the INI1-IBD density. The two IN dimers (1 and 2) are related by a twofold axis perpendicular to the sheet. Each IN monomer (A and B) is represented in a different color (blue and gold). The two monomers related by the two fold symmetry are represented in light and dark color (light blue for A1 and dark blue for A2, light gold for B1 and dark gold for B2). The positions of the IN domains are indicated: Nt for the N-terminal domain, Cc for the catalytic core and Ct for the C-terminal domain.</p

Comparative analysis of the in vitro concerted integration catalyzed by HIV-1 IN, HIV-1 IN/LEDGF and HIV-1 IN/LEDGF/INI1-IBD complex.

<p><b>A.</b> In vitro integration profiles of HIV-1 IN and HIV-1 IN/LEDGF complexes. Concerted integration assay was performed using 0.1 to 12.5µM IN or IN/LEDGF complex (concentration are normalized to IN monomers), 150 ng of acceptor plasmid, 15 ng of radioactively labelled and processed donor DNA. The reaction products were loaded on 1% agarose gel. The position and the structure of the different products obtained after half-site (HSI), full-site (FSI) and donor/donor (d/d) integration are reported. <b>B.</b> Full site integration activity. The circular FSI products obtained in (A) were quantified by cloning in bacteria and reported as the number of ampicillin-, kanamycin- and tetracycline-resistant selected clones <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060734#pone.0060734-Lesbats2" target="_blank">[62]</a>. <b>C.</b> Structure of the integration products. One hundred circular FSI integration products were isolated in condition where the concerted integration assay was performed with 12.5 µM of IN or IN/LEDGF complexes and were sequenced as reported in the materials and methods section. The number of correct 5 bp duplication, deletions and other structures are reported as percentages. All values reported are the mean ± standard deviation (error bars) of three independent sets of experiments. <b>D.</b> Effect of INI1 on the integration profile. The assay was performed and reported as in (A) using 12.5µM IN, IN/LEDGF or IN/LEDGF/INI1-IBD complex (in IN monomers). A quantification of the gel showing the strong decrease in linear fsi and dd product is shown in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060734#pone.0060734.s008" target="_blank">figure S8</a></b>. <b>E</b> Proposed equilibrium between the different complexes involved. In the presence of tDNA, INI1 is slowly displaced from the IN/LEDGF/INI1-IBD/vDNA complex and the integration reaction can occur to produce specific integration products.</p

In vitro functional tests.

<p><b>A–B:</b> Determination by fluorescence anisotropy of the dissociation constant of viral DNA to IN/LEGDF and IN/LEDGF/INI1-IBD. <b>A</b>: Titration curve of viral DNA by the IN/LEDGF complex in the absence (square, continuous line) or in the presence of an excess of non-fluorescent viral DNA (circles, dotted line) and non-fluorescent non-specific DNA (triangles, dashed line). <b>B</b>: Same as A for the IN/LEDGF/INI1-IBD complex. Lines correspond to the fits of equation 8 in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060734#pone.0060734.s012" target="_blank">methods S1</a></b> to the data. <b>C</b>: 3′ processing reaction followed by fluorescence anisotropy. The fraction of released GT as a function of time is shown in blue for the IN/LEDGF complex and in red for the IN/LEDGF/INI1-IBD complex.</p