A Novel Bioactive Peptide, T14, Selectively Activates mTORC1 Signalling: Therapeutic Implications for Neurodegeneration and Other Rapamycin-Sensitive Applications

T14 modulates calcium influx via the α-7 nicotinic acetylcholine receptor to regulate cell growth. Inappropriate triggering of this process has been implicated in Alzheimer\u27s disease (AD) and cancer, whereas T14 blockade has proven therapeutic potential in in vitro, ex vivo and in vivo models of these pathologies. Mammalian target of rapamycin complex 1 (mTORC1) is critical for growth, however its hyperactivation is implicated in AD and cancer. T14 is a product of the longer 30mer-T30. Recent work shows that T30 drives neurite growth in the human SH-SY5Y cell line via the mTOR pathway. Here, we demonstrate that T30 induces an increase in mTORC1 in PC12 cells, and ex vivo rat brain slices containing substantia nigra, but not mTORC2. The increase in mTORC1 by T30 in PC12 cells is attenuated by its blocker, NBP14. Moreover, in post-mortem human midbrain, T14 levels correlate significantly with mTORC1. Silencing mTORC1 reverses the effects of T30 on PC12 cells measured via AChE release in undifferentiated PC12 cells, whilst silencing mTORC2 does not. This suggests that T14 acts selectively via mTORC1. T14 blockade offers a preferable alternative to currently available blockers of mTOR as it would enable selective blockade of mTORC1, thereby reducing side effects associated with generalised mTOR blockade

Efficiency Consideration for Data Packets Encryption within Wireless VPN Tunneling for Video Streaming

With the help of the Internet today we can communicate with anyone from anyplace to access all types of data with a high level of QoS. This mobility is available for legitimate users, as well as for illegitimate ones, for this reason we need extra data security. A solution for QoS and data confidentiality is Virtual Private Network (VPN); ways, in which we can reduce operational costs, grow productivity, simplify network topology and extend the area of connectivity. Video data packets must arrive with a constant and low delay at the same rate in order to have e real time transmission. This paper presents an analysis of different protocols used and the way that video data packets are encapsulated and encrypted for a high level of QoS in a VPN connection

Acute cholecystitis in elderly patients, peculiarities of diagnosis and treatment

Conducător ştiinţific Iacub Vladimir A. Dr. conf. univ. Catedra Chirurgie Generală-Semiologie USMF “Nicolae Testemiţanu”.125 patients treated in surgical clinic, Municipal Hospital Chişinău No. 1 from January 2008 to December 2009 were included in the study. The aim of the study was comparative analyzsis between methods of diagnosis and treatment in geriatric patients. Was established, that the most effective method of diagnosis is ultrasound examination, which it was used in most cases. Another methods were used with the aim to acurately put the diagnosis. After surgical treatment the best results were achieved in cases with active tactics. Laparoscopic cholecystectomy is a safe procedure and should be recommended for geriatric patients who have acute cholecystitis due to cholelithiasis before the development of complications

Dictionary of Romanian Language (Letter N) - DLR (ELEXIS)

Dicționarul Limbii Române, abbreviated DLR, also called Thesaurus Dictionary of the Romanian Language, is the most important lexicographical work of the Romanian language, developed under the aegis of the Romanian Academy during more than a century. It was compiled and edited in two stages (known under the brand DA series during 1906–1944 and new series, DLR, from 1965 to 2010), in 37 volumes and contains about 175,000 words and variations, with more than 1,300,000 quotes. The development of electronic version was made in 2007–2010

The coming age of pervasive data processing

Emerging Big Data analytics and machine learning applications require a significant amount of computational power. While there exists a plethora of large-scale data processing frameworks which thrive in handling the various complexities of data-intensive workloads, the ever-increasing demand of applications have made us reconsider the traditional ways of scaling (e.g., scale-out) and seek new opportunities for improving the performance. In order to prepare for an era where data collection and processing occur on a wide range of devices, from powerful HPC machines to small embedded devices, it is crucial to investigate and eliminate the potential sources of inefficiency in the current state of the art platforms. In this paper, we address the current and upcoming challenges of pervasive data processing and present directions for designing the next generation of large-scale data processing systems.</p

A Novel Bioactive Peptide, T14, Selectively Activates mTORC1 Signalling: Therapeutic Implications for Neurodegeneration and Other Rapamycin-Sensitive Applications

T14 modulates calcium influx via the α-7 nicotinic acetylcholine receptor to regulate cell growth. Inappropriate triggering of this process has been implicated in Alzheimer’s disease (AD) and cancer, whereas T14 blockade has proven therapeutic potential in in vitro, ex vivo and in vivo models of these pathologies. Mammalian target of rapamycin complex 1 (mTORC1) is critical for growth, however its hyperactivation is implicated in AD and cancer. T14 is a product of the longer 30mer-T30. Recent work shows that T30 drives neurite growth in the human SH-SY5Y cell line via the mTOR pathway. Here, we demonstrate that T30 induces an increase in mTORC1 in PC12 cells, and ex vivo rat brain slices containing substantia nigra, but not mTORC2. The increase in mTORC1 by T30 in PC12 cells is attenuated by its blocker, NBP14. Moreover, in post-mortem human midbrain, T14 levels correlate significantly with mTORC1. Silencing mTORC1 reverses the effects of T30 on PC12 cells measured via AChE release in undifferentiated PC12 cells, whilst silencing mTORC2 does not. This suggests that T14 acts selectively via mTORC1. T14 blockade offers a preferable alternative to currently available blockers of mTOR as it would enable selective blockade of mTORC1, thereby reducing side effects associated with generalised mTOR blockade