For Whom Is the Path the Goal? A Lifespan Perspective on the Development of Goal Focus

Goals are an intensely studied concept in various research areas within psychology. They can be defined as cognitive representations of means-ends relations. The relative focus on the means or the ends (i.e., goal focus) can vary between persons and over time. Taking a lifespan perspective, we use the existing developmental, social-cognitive, and motivational literature to portray how goal focus might develop across the entire lifespan. For this purpose, we take findings on the perception of goal-directed behavior in infancy, the development of (self-)representations and goal pursuit in adolescence, and of goals across adulthood into account. We propose that goal focus changes across the lifespan due to age-related cognitive and motivational development, and that the relative impact of cognitive and motivational processes on goal focus varies across the lifespan. We conclude by integrating different approaches and findings from a lifespan perspective

Chasing the “How” and “Why” of goal pursuit: A multimethod approach to the study of goal focus

Goals are cognitive representations of means-ends relations that reflect what a person wants to approach or avoid. Previous research has demonstrated that the relative salience of the means and ends (i.e., goal focus) differs across adulthood. Due to inconsistent findings in recent studies, this study systematically investigated the convergence of goal focus across different dimensions (i.e., goal content, complexity, type, i.e., hypothetical vs. personal goals, and method of assessment) and its relation to age. To this end, we conducted a multimethod online study (N = 773) across an age range from 14 to 87 years. The results provide little support for the convergence of goal focus across different assessment methods and systematic associations with age. We discuss the implications of these findings for goal research

Systems-level organization of yeast methylotrophic lifestyle

BACKGROUND: Some yeasts have evolved a methylotrophic lifestyle enabling them to utilize the single carbon compound methanol as a carbon and energy source. Among them, Pichia pastoris (syn. Komagataella sp.) is frequently used for the production of heterologous proteins and also serves as a model organism for organelle research. Our current knowledge of methylotrophic lifestyle mainly derives from sophisticated biochemical studies which identified many key methanol utilization enzymes such as alcohol oxidase and dihydroxyacetone synthase and their localization to the peroxisomes. C1 assimilation is supposed to involve the pentose phosphate pathway, but details of these reactions are not known to date. RESULTS: In this work we analyzed the regulation patterns of 5,354 genes, 575 proteins, 141 metabolites, and fluxes through 39 reactions of P. pastoris comparing growth on glucose and on a methanol/glycerol mixed medium, respectively. Contrary to previous assumptions, we found that the entire methanol assimilation pathway is localized to peroxisomes rather than employing part of the cytosolic pentose phosphate pathway for xylulose-5-phosphate regeneration. For this purpose, P. pastoris (and presumably also other methylotrophic yeasts) have evolved a duplicated methanol inducible enzyme set targeted to peroxisomes. This compartmentalized cyclic C1 assimilation process termed xylose-monophosphate cycle resembles the principle of the Calvin cycle and uses sedoheptulose-1,7-bisphosphate as intermediate. The strong induction of alcohol oxidase, dihydroxyacetone synthase, formaldehyde and formate dehydrogenase, and catalase leads to high demand of their cofactors riboflavin, thiamine, nicotinamide, and heme, respectively, which is reflected in strong up-regulation of the respective synthesis pathways on methanol. Methanol-grown cells have a higher protein but lower free amino acid content, which can be attributed to the high drain towards methanol metabolic enzymes and their cofactors. In context with up-regulation of many amino acid biosynthesis genes or proteins, this visualizes an increased flux towards amino acid and protein synthesis which is reflected also in increased levels of transcripts and/or proteins related to ribosome biogenesis and translation. CONCLUSIONS: Taken together, our work illustrates how concerted interpretation of multiple levels of systems biology data can contribute to elucidation of yet unknown cellular pathways and revolutionize our understanding of cellular biology. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12915-015-0186-5) contains supplementary material, which is available to authorized users

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Chasing the “How” and “Why” of goal pursuit: A multimethod approach to the study of goal focus

Goals are cognitive representations of means-ends relations that reflect what a person wants to approach or avoid. Previous research has demonstrated that the relative salience of the means and ends (i.e., goal focus) differs across adulthood. Due to inconsistent findings in recent studies, this study systematically investigated the convergence of goal focus across different dimensions (i.e., goal content, complexity, type, i.e., hypothetical vs. personal goals, and method of assessment) and its relation to age. To this end, we conducted a multimethod online study (N = 773) across an age range from 14 to 87 years. The results provide little support for the convergence of goal focus across different assessment methods and systematic associations with age. We discuss the implications of these findings for goal research

Colonial fault lines : First Nations autonomy and Indigenous lands in the time of COVID-19

The COVID-19 crisis has exposed the political and economic fault lines in the exercise of power across multiple jurisdictions. This article focuses on the power of First Nations to make enforceable decisions in respect to reserve lands. We argue that Canadian law both enables First Nations to assert jurisdiction in respect to their lands, yet undermines Indigenous authority in relation to enforcement and intergovernmental status.Law, Peter A. Allard School ofReviewedFacult

Colonial Fault Lines: First Nations Autonomy and Indigenous Lands in the Time of COVID-19

The COVID-19 crisis has exposed the political and economic fault lines in the exercise of power across multiple jurisdictions. This article focuses on the power of First Nations to make enforceable decisions in respect to reserve lands, specifically the powers First Nations have to enforce public health restrictions during the pandemic. We argue that Canadian law both enables First Nations to assert decisionmaking in respect to their lands, and undermines Indigenous authority in relation to enforcement and intergovernmental status. This paper is part of the SPE Theme on the Political Economy of COVID-19

Spelling out some unaddressed conceptual and methodological challenges in empirical lifespan research

The importance of taking a lifespan approach to describe and understand human development has long been acknowledged (e.g., Baltes, 1987). Nevertheless, theoretical or empirical research that actually encompasses the entire lifespan, that is, from early childhood to old age, is rare. This is not surprising given the challenges such an approach entails. Many of these challenges (e.g., establishing measurement invariance between age groups) have been addressed in the previous literature, but others have not yet been sufficiently considered. The main purpose of this article is to present several examples of such largely unaddressed conceptual and methodological challenges and reflect upon possible ways to address them. We discuss the usefulness of a lifespan approach and the generalization of the challenges to other research comparing different groups, such as gender, culture, or species