The Multifunctional Building

Import 02/11/2016Import 20/01/2016Import 11/02/2016Obsahem této práce je návrh polyfunkčního domu včetně vytápění ve stupni projektové dokumentace pro provádění stavby. Z počátku se práce zabývá problematikou pozemního stavitelství. Následně je zaměřena na návrh a výpočet otopné soustavy, zdroje tepla a dalších prvků potřebných k její správné funkčnosti. Je zde také zohledněna potřeba teplé vody pro uživatele a návštěvníky objektu.The contents of this work are the design of a multifunctional house including heating systems. The design is a complete project ready for realization of the building. At the beginning the work concentrates on the issues of the building and engineering. Consequently, it is aimed at the design and calculations of the heating systém, sources of heat and other elements necessary for its correct functioning. Necessity of warm water for users and visitors to the site has been taken into account as well.229 - Katedra prostředí staveb a TZBvelmi dobř

Application of Facility Management in the life cycle of buildings

Import 26/06/2013Tématem této bakalářské práce je Uplatnění Facility managementu v životním cyklu staveb. Obsah této bakalářské práce se skládá ze dvou základních částí. Jedná se o teoretickou část, kde jsou popsány jednotlivé pojmy týkající se Facility managementu a životního cyklu stavby. Dále jsou zde zmíněny výhody vyplývající jejich skloubením v jednotlivých fázích životního cyklu stavby. Pro následující praktickou část byla vybrána zrealizovaná stavba, ve které proběhl průzkum z hlediska působnosti Facility managementu. Po této analýze byly vybrány a popsány návrhy na zlepšení chodu podpůrných činností společnosti sídlící v ní. Dokončením této práce byl zjištěn rozsah využití FM na podporu hlavního předmětu podnikání v středně velké společnosti na území České republiky. Rozsah této bakalářské práce je 43 číslovaných stran.The theme of this bachelor work is Application of Facility Management in the Life Cycle of Buildings. The contents of this work consist of two basic parts. A theoretical part where individual terms concerning Facility Management and the Life Cycle of Buildings are described is one of them. Later, there are advantages arising from their combination in individual phases of a life cycle of buildings mentioned. For the following practical part, an implemented building was chosen where a poll was done from the point of view of the effects of Facility management. Suggestions of how to improve the procedures of supporting activities of companies residing there were chosen and described after this analysis. To what degree it is possible to use Facility management as a support of the main object of running a business in a medium sized company in the Czech Republic is in the completion of the work. The size of this work is 43 numbered pages.Prezenční222 - Katedra městského inženýrstvídobř

Activity in perirhinal and entorhinal cortex predicts perceived visual similarities among category exemplars with highest precision

Vision neuroscience has made great strides in understanding the hierarchical organization of object representations along the ventral visual stream (VVS). How VVS representations capture fine-grained visual similarities between objects that observers subjectively perceive has received limited examination so far. In the current study, we addressed this question by focusing on perceived visual similarities among subordinate exemplars of real world-categories. We hypothesized that these perceived similarities are reflected with highest fidelity in neural activity patterns downstream from inferotemporal regions, namely in perirhinal and anterolateral entorhinal cortex in the medial temporal-lobe. To address this issue with fMRI, we administered a modified 1-Back task that required discrimination between category exemplars as well as categorization. Further, we obtained observer-specific ratings of perceived visual similarities, which predicted behavioural performance during scanning. As anticipated, we found that activity patterns in perirhinal and anterolateral entorhinal cortex predicted the structure of perceived visual similarity relationships among category exemplars, including its observer-specific component, with higher precision than any other VVS region. Our findings provide new evidence that subjective aspects of object perception that rely on fine-grained visual differentiation are reflected with highest fidelity in the medial temporal lobe

Preclinical Evaluation of a Replication-Deficient Intranasal ΔNS1 H5N1 Influenza Vaccine

We developed a novel intranasal influenza vaccine approach that is based on the construction of replication-deficient vaccine viruses that lack the entire NS1 gene (ΔNS1 virus). We previously showed that these viruses undergo abortive replication in the respiratory tract of animals. The local release of type I interferons and other cytokines and chemokines in the upper respiratory tract may have a “self-adjuvant effect”, in turn increasing vaccine immunogenicity. As a result, ΔNS1 viruses elicit strong B- and T- cell mediated immune responses.), one dose of vaccine delivered intranasally was sufficient for the induction of antibodies against homologous A/Vietnam/1203/04 and heterologous A/Indonesia/5/05 H5N1 strains.Our findings show that intranasal immunization with the replication deficient H5N1 ΔNS1 vaccine candidate is sufficient to induce a protective immune response against H5N1 viruses. This approach might be attractive as an alternative to conventional influenza vaccines. Clinical evaluation of ΔNS1 pandemic and seasonal influenza vaccine candidates are currently in progress

Single HA2 Mutation Increases the Infectivity and Immunogenicity of a Live Attenuated H5N1 Intranasal Influenza Vaccine Candidate Lacking NS1

Our finding suggests that an efficient intranasal vaccination with a live attenuated H5N1 virus may require a certain level of pH and temperature stability of HA in order to achieve an optimal virus uptake by the nasal epithelial cells and induce a sufficient immune response. The pH of the activation of the H5 HA protein may play a substantial role in the infectivity of HPAIVs for mammals

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Surgical Technique in Distal Pancreatectomy: A Systematic Review of Randomized Trials

Despite recent improvements in surgical technique, the morbidity of distal pancreatectomy remains high, with pancreatic fistula being the most significant postoperative complication. A systematic review of randomized controlled trials (RCTs) dealing with surgical techniques in distal pancreatectomy was carried out to summarize up-to-date knowledge on this topic. The Cochrane Central Registry of Controlled Trials, Embase, Web of Science, and Pubmed were searched for relevant articles published from 1990 to December 2013. Ten RCTs were identified and included in the systematic review, with a total of 1286 patients being randomized (samples ranging from 41 to 450). The reviewers were in agreement for application of the eligibility criteria for study selection. It was not possible to carry out meta-analysis of these studies because of the heterogeneity of surgical techniques and approaches, such as varying methods of pancreas transection, reinforcement of the stump with seromuscular patch or pancreaticoenteric anastomosis, sealing with fibrin sealants and pancreatic stent placement. Management of the pancreatic remnant after distal pancreatectomy is still a matter of debate. The results of this systematic review are possibly biased by methodological problems in some of the included studies. New well designed and carefully conducted RCTs must be performed to establish the optimal strategy for pancreatic remnant management after distal pancreatectomy