Avaliação clínica de uma resina flow como uma camada intermediária em restaurações de lesões cervicais não cariosas: 48 meses

PURPOSE: In this case report, the clinical performance of a microhybrid resin composite placed with or without a flowable resin composite was compared, over a 48-month period. CASE DESCRIPTION: The patient of this case report presented 2 pairs of equivalent cervical abfraction lesions, under occlusion. Four restorations were placed in teeth 34, 35, 44 and 45. The restorations were divided into groups (Single Bond + Filtek-Flow + Filtek Z250 or Single Bond + Filtek Z250) and the materials were applied according to the manufactures instructions. Two previously calibrated operators placed the restorations and two other independent examiners evaluated the restorations at baseline and after 48 months, according to the USPHS criteria and modified criteria for color match. CONCLUSION: After 48 months of evaluation the lesions restored with Filtek-Flow as a liner under Filtek Z250 did not show better clinical performance than the restorations without Filtek-Flow. All restorations showed a trend toward dark yellowing after 48 months.OBJETIVO: Este relato de caso compara o desempenho clínico após 48 meses de restaurações de lesões cervicais não cariosas com uma resina composta microhíbrida associada ou não a uma camada de resina flow como um agente intermediário. DESCRIÇÃO DO CASO: O paciente do presente caso apresentava 2 pares de lesões cervicais não cariosas ocasionadas por abfração sob oclusão. Nos elementos dentários 35 e 44 as restaurações foram feitas com Single Bond + Filtek-Flow + Filtek Z250 e nos elementos 45 e 34 com Single Bond + Filtek Z250, sendo os materiais empregados de acordo com as recomendações do fabricante. Dois operadores previamente calibrados colocaram as restaurações e dois outros examinadores avaliaram as restaurações no período imediato (baseline) e após 48 meses, de acordo os critérios USPHS modificado para o critério cor. CONCLUSÃO: Após 48 meses as lesões restauradas com a resina flow como uma camada intermediária não demonstraram melhor desempenho clínico em relação às restaurações sem a resina flow. Todos os grupos apresentaram uma tendência à descoloração após 48 meses de acompanhamento clínico

Evaluating resin-enamel bonds by microshear and microtensile bond strength tests: effects of composite resin

OBJECTIVES: The aims of this study were to evaluate the effect of resin composite (Filtek Z250 and Filtek Flow Z350) and adhesive system [(Solobond Plus, Futurabond NR (VOCO) and Adper Single Bond (3M ESPE)] on the microtensile (&#956;TBS) and microshear bond strength (&#956;SBS) tests on enamel, and to correlate the bond strength means between them. MATERIAL AND METHODS: Thirty-six extracted human molars were sectioned to obtain two tooth halves: one for &#956;TBS and the other one for &#956;SBS. Adhesive systems and resin composites were applied to the enamel ground surfaces and light-cured. After storage (37(0)C/24 h) specimens were stressed (0.5 mm/min). Fracture modes were analyzed under scanning electron microscopy. The data were analyzed using two-way ANOVA and Tukey's test (&#945;=0.05). RESULTS: The correlation between tests was estimated with Pearson's product-moment correlation statistics (&#945; =0.05). For both tests only the main factor resin composite was statistically significant (p<0.05). The correlation test detected a positive (r=0.91) and significant (p=0.01) correlation between the tests. CONCLUSIONS: The results were more influenced by the resin type than by the adhesives. Both microbond tests seem to be positive and linearly correlated and can therefore lead to similar conclusions.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Effect of the application time of phosphoric acid and self-etch adhesive systems to sclerotic dentin

Objective: To evaluate the effect of application time on the resin-dentin bond strength (µTBS) and etching pattern of adhesive systems applied on sclerotic dentine. Material and Methods: A total of forty-two bovine incisors had their roots removed. The 1-step self-etch GO (SDI), the 2-step self-etch Adper SE Bond (3MESPE) and the 35% phosphoric acid (3MESPE) from the 2-step etch-and-rinse Adper Single Bond 2 (3MESPE) were applied on the bovine incisal surfaces according to the manufacturer's instructions or duplicating the recommended conditioning time. After adhesive application, thirty teeth were restored with composite resin, stored for 24 h in distilled water at 37°C, and sectioned into resin-dentin bonded sticks (0.8 mm 2 ) and tested according to the µTBS at 0.5 mm/min. The etching pattern of the remaining twelve teeth (n=4 for each material) was examined under scanning electron microscopy. Each tooth was divided into a buccal-to-lingual direction into three thirds, and each third randomly assigned to the groups: control (no treatment), according to the manufacturers' instructions and duplicating the recommended application time. The µTBS and the relative percentage of the tubule area opening were evaluated by two-way repeated measures ANOVA and Tukey's tests (α=0.05). Results: The duplication of the conditioning time favored only the GO adhesive (

Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth

The aim of this study was to assess, using the DPPH assay, the antioxidant activity of several substances that could be proposed to immediately revert the problems caused by bleaching procedures. The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize® (NE), Desensibilize® (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine® (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p<0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p<0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants

KIAA1549: BRAF gene fusion and FGFR1 hotspot mutations are prognostic factors in pilocytic astrocytomas

Up to 20% of patients with pilocytic astrocytoma (PA) experience a poor outcome. BRAF alterations and Fibroblast growth factor receptor 1 (FGFR1) point mutations are key molecular alterations in Pas, but their clinical implications are not established. We aimed to determine the frequency and prognostic role of these alterations in a cohort of 69 patients with PAs. We assessed KIAA1549:BRAF fusion by fluorescence in situ hybridization and BRAF (exon 15) mutations by capillary sequencing. In addition, FGFR1 expression was analyzed using immunohistochemistry, and this was compared with gene amplification and hotspot mutations (exons 12 and 14) assessed by fluorescence in situ hybridization and capillary sequencing. KIAA1549:BRAF fusion was identified in almost 60% of cases. Two tumors harbored mutated BRAF. Despite high FGFR1 expression overall, no cases had FGFR1 amplifications. Three cases harbored a FGFR1 p.K656E point mutation. No correlation was observed between BRAF and FGFR1 alterations. The cases were predominantly pediatric (87%), and no statistical differences were observed in molecular alterations-related patient ages. In summary, we confirmed the high frequency of KIAA1549:BRAF fusion in PAs and its association with a better outcome. Oncogenic mutations of FGFR1, although rare, occurred in a subset of patients with worse outcome. These molecular alterations may constitute alternative targets for novel clinical approaches, when radical surgical resection is unachievable.This study was partially supported by CNPq/Universal (475358/2011-2), and FAPESP (2012/19590-0) grants to RMR and to the NIH- P30CA046934 (CCSG Molecular Pathology/Cytogenetics) to MVG and DL

EFECTO DE LA SUPLEMENTACIÓN ALIMENTICIA CON FUENTES LIPÍDICAS SOBRE LA GANANCIA DE PESO Y NIVELES DE TESTOSTERONA SÉRICA EN TOROS BUFALINOS / EFFECT OF NUTRITIONAL SUPPLEMENTATION WITH LIPIDS ON WEIGHT GAIN AND TESTOSTERONE LEVELS OF WATER BUFFALO BULLS

El objetivo del estudio fue evaluar el uso de concentrados a base de torta de copra y torta de palmiste en la suplementación de toros bufalinos mantenidos en pastoreo rotacional intensivo y comprobar su efecto sobre el peso corporal, el perímetro escrotal y los niveles de testosterona sérica. Quince búfalos (457,8±98,3 kg y 3,2±1,3 años) fueron tenidos en pastoreo (Panicum maximum vr. Mombaza) y recibieron suplementación diaria (1% del peso corporal) en comederos individuales. Los animales fueron asignados aleatoriamente en tres tratamientos: Control (n = 5; concentrado convencional, a base de maíz y salvado de soya), T1 (n = 5; concentrado a base de torta de copra) y T2 (n = 5; concentrado a base de torta de palmiste). El experimento duró 252 días, subdivididos en 9 periodos de 28 días (P1 a P9). El consumo de la ración fue controlado diariamente, el peso corporal y el perímetro escrotal cada 28 días (P1 a P9), y los niveles de testosterona plasmática cada 14 días (P7 a P9). La ganancia de peso promedio varió de 0,92 a 0,97 kg/animal/día. El peso corporal y el perímetro escrotal promedio no mostraron diferencias (P>0,05). Los animales del T2 presentaron niveles medios de testosterona más elevados (2,2 ng/mL) frente a Control (1,8 ng/mL) y a T1 (1,6 ng/mL) (P0.05). Animals in T2 had higher average levels of testosterone (2.2 ng/mL) compared to Control (1.8 ng/mL) and T1 (1.6 ng/mL) (P<0.05). Positive correlations were observed between body weight and testosterone levels (r = 0.58; P<0.0001) and between testosterone and scrotal circumference (r = 0.16; P<0.02). In conclusion, the new dietary supplements promoted performance similar to the conventional concentrate, considering the body weight gain and the testicular biometry in young buffalo bulls. However, the use of palm kernel cake was more efficient in raising serum testosterone levels

Insertion of nursing in diagnostic technologies: innovations in secondary health care / Inserção da enfermagem nas tecnologias diagnósticas: inovações em atenção secundária de saúde

Objetivo: Desenvolver Procedimentos Operacionais Padrão (POP) para a assistência de enfermagem na realização de exames diagnósticos na atenção secundária à saúde. Método: Elaborou-se uma comissão de Sistematização da Assistência de Enfermagem (SAE), que avaliou os setores em que estavam sendo implantadas novas tecnologias diagnósticas e levantou a necessidade de treinamento da equipe técnica que realizava estes exames para padronizar os procedimentos, diminuindo os erros e falhas em sua realização. Os POPs foram descritos através de quadros compostos por: definição do procedimento diagnóstico, material necessário, intervenções de enfermagem e cuidados especiais. Resultados: Foram elaborados quatro POPs, relacionados aos exames de: espirometria, polissonografia, retinografia e teste do hidrogênio expirado. Conclusão: Através do estudo foi possível não somente conhecer a execução dos exames, mas também entender a aplicabilidade da SAE com foco nas tecnologias diagnósticas, além de fornecer subsídios para futura implementação da SAE na instituição de estudo

Insertion of nursing in diagnostic technologies: innovations in secondary health care / Inserção da enfermagem nas tecnologias diagnósticas: inovações em atenção secundária de saúde

Objetivo: Desenvolver Procedimentos Operacionais Padrão (POP) para a assistência de enfermagem na realização de exames diagnósticos na atenção secundária à saúde. Método: Elaborou-se uma comissão de Sistematização da Assistência de Enfermagem (SAE), que avaliou os setores em que estavam sendo implantadas novas tecnologias diagnósticas e levantou a necessidade de treinamento da equipe técnica que realizava estes exames para padronizar os procedimentos, diminuindo os erros e falhas em sua realização. Os POPs foram descritos através de quadros compostos por: definição do procedimento diagnóstico, material necessário, intervenções de enfermagem e cuidados especiais. Resultados: Foram elaborados quatro POPs, relacionados aos exames de: espirometria, polissonografia, retinografia e teste do hidrogênio expirado. Conclusão: Através do estudo foi possível não somente conhecer a execução dos exames, mas também entender a aplicabilidade da SAE com foco nas tecnologias diagnósticas, além de fornecer subsídios para futura implementação da SAE na instituição de estudo

Comparison of erosion rates in forest roads applying the WEPP model (Water Erosion Prediction Project) modified with reference to experimental measurements

O objetivo do trabalho foi testar o modelo WEPP (Water Erosion Prediction Project), através de comparações entre volume de enxurrada e perda de solo observados experimentalmente, provenientes dos segmentos de estradas florestais submetidas à chuva natural com inclinações de 1 e 7% e comprimentos de rampa de 20 e 40 m, e aqueles preditos pelo aplicativo, visando o desenvolvimento de um modelo brasileiro de predição de erosão em estradas florestais. Na determinação da quantidade do material erodido foram instalados tambores coletores, com capacidade de 209,25 litros, localizados na parte inferior das estradas, onde foram inseridas tubulações de PVC de 2 polegadas para coleta dos sedimentos provenientes da estrada propriamente dita. Nos tambores coletores foram feitos orifícios nivelados e perfeitamente iguais, posicionados a 0,65 m do fundo do primeiro e a 0,60 m do fundo do segundo, que funcionaram como um divisor Geib. Nas parcelas de 20 e 40 m de comprimento foram feitos cinco e sete orifícios, respectivamente, no primeiro e segundo tambores. O terceiro tambor foi utilizado para coletar o excedente da enxurrada proveniente do segundo tambor. Os tambores foram ligados em série, através de cano PVC de 2 polegadas. Os dados de volume e intensidade de precipitação diária foram obtidos com a instalação de pluviômetro e pluviógrafo no local. O período de coleta de dados foi de um ano, concentrando-se na época das chuvas. Posteriormente, os arquivos de clima, precipitação, solo, inclinação e comprimento do segmento foram introduzidos e adaptados ao modelo de predição de erosão WEPP com o propósito de testá-lo, visando a confecção de um modelo apropriado às condições brasileiras. _________________________________________________________________________________________ ABSTRACT: The objective of this work was to test the WEPP model (Water Erosion Prediction Project), by comparing experimentally observed runoff volume and soil loss in forest road segments under normal rainfall, with 1 and 7% slopes and lengths of 20 and 40 m, and those predicted by the WEPP, aiming to develop a Brazilian model of erosion prediction on forest roads. To determine the amount of eroded matter, 209.25 l capacity collector drums were placed on the lower level of the road segment. PVC pipes of 2 inches were connected to the drums to collect sediments from the road. Openings at the same height and size were made on the drums, located at 0.65 m from the bottom of the first drum and at 0.60 m from the bottom of the second drum, which functioned as a Geib divisor. In the 20 and 40 m long plots five and seven openings were made, respectively, on the first and second drums, with the third drum being used to collect the runoff excess from the second drum. The drums were connected in series through 2 inch - PVC pipes. Data of volume and intensity of daily precipitation were obtained by means of pluviometers and pluviographs. The period of data collection was one year, concentrated in the rainy season. Files on climate, precipitation, soil, slope and segment length were introduced and adapted to the WEPP model erosion prediction model to test it, aiming to elaborate a model suited to the Brazilian forest conditions

Neotropical Freshwater Fishes: A dataset of occurrence and abundance of freshwater fishes in the Neotropics

The Neotropical region hosts 4225 freshwater fish species, ranking first among the world's most diverse regions for freshwater fishes. Our NEOTROPICAL FRESHWATER FISHES data set is the first to produce a large-scale Neotropical freshwater fish inventory, covering the entire Neotropical region from Mexico and the Caribbean in the north to the southern limits in Argentina, Paraguay, Chile, and Uruguay. We compiled 185,787 distribution records, with unique georeferenced coordinates, for the 4225 species, represented by occurrence and abundance data. The number of species for the most numerous orders are as follows: Characiformes (1289), Siluriformes (1384), Cichliformes (354), Cyprinodontiformes (245), and Gymnotiformes (135). The most recorded species was the characid Astyanax fasciatus (4696 records). We registered 116,802 distribution records for native species, compared to 1802 distribution records for nonnative species. The main aim of the NEOTROPICAL FRESHWATER FISHES data set was to make these occurrence and abundance data accessible for international researchers to develop ecological and macroecological studies, from local to regional scales, with focal fish species, families, or orders. We anticipate that the NEOTROPICAL FRESHWATER FISHES data set will be valuable for studies on a wide range of ecological processes, such as trophic cascades, fishery pressure, the effects of habitat loss and fragmentation, and the impacts of species invasion and climate change. There are no copyright restrictions on the data, and please cite this data paper when using the data in publications.Fil: Tonella, Lívia Helena. Universidade Estadual de Maringá. Departamento de Engenharia Química. Laboratorio de Pesquisa.; BrasilFil: Ruaro, Renata. Universidade Estadual de Maringá. Departamento de Engenharia Química. Laboratorio de Pesquisa.; BrasilFil: Daga, Vanessa Salete. Universidade Federal do Paraná; BrasilFil: Garcia, Diego Azevedo Zoccal. Universidade Estadual de Londrina; BrasilFil: Barroso Vitorino Júnior, Oscar. Instituto Natureza do Tocantins-Naturatins; BrasilFil: Lobato de Magalhães, Tatiana. Universidad Autonoma de Queretaro.; MéxicoFil: Reis, Roberto Esser. Museu de Ciências e Tecnologia; BrasilFil: Di Dario, Fabio. Universidade Federal do Rio de Janeiro; BrasilFil: Petry, Ana Cristina. Universidade Federal do Rio de Janeiro; BrasilFil: Mincarone, Michael Maia. Universidade Federal do Rio de Janeiro; BrasilFil: Assis Montag, Luciano Fogaça. Universidade Federal do Pará; BrasilFil: Pompeu, Paulo Santos. Universidade Federal de Lavras; BrasilFil: Teixeira, Adonias Aphoena Martins. Universidade Estadual da Paraiba; BrasilFil: Carmassi, Alberto Luciano. Universidade Federal de Sao Paulo; Brasil. Universidade Federal do São Carlos; BrasilFil: Sánchez, Alberto J.. Universidad Juárez Autónoma de Tabasco; MéxicoFil: Giraldo Pérez, Alejandro. Universidade Federal de Minas Gerais; BrasilFil: Bono, Alessandra. Universidad de Vale do Rio dos Sinos; BrasilFil: Datovo, Aléssio. Universidade de Sao Paulo; BrasilFil: Flecker, Alexander S.. Cornell University; Estados UnidosFil: Sanches, Alexandra. Universidade de Sao Paulo; Brasil. Universidade Federal do São Carlos; BrasilFil: Godinho, Alexandre Lima. Universidade Federal de Minas Gerais; BrasilFil: Matthiensen, Alexandre. Embrapa Suínos e Aves; BrasilFil: Peressin, Alexandre. Universidade Federal de Lavras; BrasilFil: Silva Hilsdorf, Alexandre Wagner. Universidade de Mogi das Cruzes; BrasilFil: Barufatti, Alexéia. Universidade Federal da Grande Dourados; BrasilFil: Hirschmann, Alice. Universidade Federal do Pampa; BrasilFil: Jung, Aline. Universidade Do Estado de Mato Grosso (unemat);Fil: Cruz Ramírez, Allan K.. Universidad Juárez Autónoma de Tabasco; MéxicoFil: Braga Silva, Alline. Instituto Federal de Goiás; BrasilFil: Cunico, Almir Manoel. Universidade Federal do Paraná; BrasilFil: Tagliaferro, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin