Cytokeratin 10 and Ki-67 Nuclear Marker Expression in Keratoacanthoma and Squamous Cell Carcinoma

The most frequent consideration in the clinical and histologic dif ferential diagnosis of keratoacanthoma is squamous cell carcinoma. In the pres ent study, cytokeratin 10 ex pres sion and pro lif er a tion rate as measured by Ki-67 ex pres sion were com pared be tween 50 clin i cally and histologically di ag nosed keratoacanthomas and 50 squamous cell car ci no mas. Tis sue sec tions from the skin were immunohistochemically stained with anti-cytokeratin 10 and anti-Ki-67 monoclonal antibodies. The distribution of cytokeratin 10 ex pres sion and proliferative cell count were analyzed. Study results showed higher cytokeratin 10 expression in kerato acanthomas than in squamous cell car cinomas and different distribution of staining in the two entities. The analysis of cytokeratin 10 ex pression showed a much wider range of values and statistically higher median (p 0.001) in kerato acanthomas than in squamous cell carcinomas. Additionally, the prolifer ation index of keratinocytes as mea sured by Ki-67 ex pres sion was significantly higher in squamous cell carcinomas than in keratoacanthomas (p 0.01). These re sults may prove help ful in histologic differentiation of these disorders

Prospektivno praćenje trudnica na monoterapiji lamotriginom u Hrvatskoj - predkoncepcijsko savjetovanje i praćenje lijekova

We prospectively surveyed 23 pregnant women with epilepsy on lamotrigine monotherapy and reported outcome of their pregnancies, including one fetal intrauterine death, one spontaneous abortion and two preterm deliveries. There were no congenital malformations in their offspring. Women with pregnancy planning and folic acid intake delivered babies with higher values of birth weight and birth length. There was large inter-patient variation during drug monitoring and in the need of dose adjustment. Individual approach to every woman and monotherapy with minimal effective lamotrigine dose with frequent drug monitoring enhances the possibility for successful pregnancy. The management of women with epilepsy should begin with pre-pregnancy counseling. Planned pregnancy enables periconceptional folic acid supplementation. Despite the small number of cases, these data indicate that lamotrigine treatment during pregnancy might be relatively safe. Larger prospective studies are needed to obtain adequate power for statistical analysis including long-term cohort studies.U ovoj studiji smo prospektivno pratili ishod trudnoće u 23 trudnice s epilepsijom koje su uzimale lamotrigin kao monoterapiju. Trudnoća je kod bolesnica rezultirala intrauterinom smrću djeteta u jednom slučaju. spontanim abortusom u jednom slučaju, te prijevremenim porodom u dva slučaja. Kod novorođenčadi nisu zabilježene kongenitalne malformacije. Žene koje su planirale trudnoću i uzimale folnu kiselinu rodile su djecu s višom tjelesnom masom i visinom. Postojala je velika različitost medu bolesnicama u praćenju doze lijeka te u potrebi za usklađivanjem doze. Veća je mogućnost uspješnog planiranja trudnoće ako se svakoj bolesnici pristupi individualno uz minimalnu djelotvornu dozu lijeka (lamotrigin). Liječenje trudnica treba započeti savjetovanjem prije začeća kada je moguće i pravodobno započeti s uzimanjem folne kiseline. Unatoč malom broju slučajeva podaci iz naše studije pokazuju kako liječenje lamotriginom tijekom trudnoće može biti relativno sigurno. Potrebne su veće prospektivne studije kako bi se postigla zadovoljavajuća statistička snaga dobivenih podataka

Utjecaj gnojidbe i sorte na komponente prinosa salate (Lactuca sativa L.)

Salata (Lactuca sativa L.) je veoma važna i široko rasprostranjena povrtna kultura. Zbog brojnih nutritivnih i ljekovitih karakteristika, salata ima značajnu ulogu u prevenciji bolesti. Biološke karakteristike salate i njen specifičan rast i razvoj predstavljaju osnovu za uspostavljanje optimalnog načina uzgoja. U cilju da se postignu odgovarajući visoki prinosi, vrši se prihrana različitim organskim, mineralnim i mikrobiološkim gnojivima. Ona imaju veliki značaj prilikom rasta i razvoja salate, a osim toga utječu i na njene kvalitativne karakteristike. Cilj istraživanja je bio utvrditi učinak primjene različitih gnojiva na kvalitetu i kvantitetu različitih sorti salate u proljetnoj proizvodnji. Postavljen je dvofaktorijalni pokus (gnojivo x sorta) po slučajnom blok sustavu u plasteniku bez grijanja, na privatnoj parceli, na lokalitetu Lukavica, područje grada Istočno Sarajevo. Tijekom istraživanja ispitivan je utjecaj gnojiva (kontrola, Slavol, Fitofert hemisuper) na dvije sorte salate (Santoro RZ i Kiribati RZ) i to na: dužinu korjena (cm), broj listova, masu nadzemnog dijela biljke (g) i prinos (kg/m2). Najveća dužina korjena zabilježena je na varijanti gnojiva s fitofertom (10,02 cm) i u usporedbi s kontrolnom varijantom (8,55 cm) razlika je bila statistički značajna. Najveći prinos (509,5 kg/100m2) ostvaren je na varijanti gnojidbe s mikrobiološkim gnojivom, a najmanji na kontrolnoj varijanti (250 kg/100 m2). Razlike u ostvarenom prinosu unutar sorti nisu bile statistički opravdane

Psychosocial influence of COVID-19 on healthcare workers

Aim To assess a psychosocial impact of the Coronavirus disease 2019 (COVID-19) on health care workers and to quantify the size of depression symptoms, anxiety and stress levels. Methods This cross-sectional study used an anonymous online survey questionnaire as a research instrument and it included 114 health workers of all profiles from the Sarajevo Canton employed in private and public institutions. The research was voluntary, non-commercial and all participants provided an oral informed consent. Depression, Anxiety and Stress Scale (DASS-21) questionnaire was used for assessing emotional status of depression, anxiety and stress. Results The mean age of participants was 40.5±8.44 years with male:female ratio of 0.28. Prevalence of depression was 46.5%, anxiety61.4%, and 36.9% stress. Age and gender had no effect on emotional status, but it was revealed that women achieved higher depression, anxiety and stress scores than men (without statistical significance). The most notable effect on the emotional state was found for direct or indirect contact with COVID-19 patients. Medical workers in direct contact with COVID-19 patients achieved greater depression (p=0.005), anxiety (p=0.001), stress (p=0.030) and total DASS-21 (p=0.003) scores. Conclusion High prevalence of health workers affected by various psychological ailments during the COVID-19 pandemic was found. This evidence underscores the need to address adverse effects of the pandemic on mental health of health care workers

Hrvatski model integrativne prospektivne skrbi trudnoća u žena s epilepsijom

Epilepsy is the most common neurological complication in pregnancy. Women with epilepsy have a higher risk of complications in pregnancy. In Croatia, women with epilepsy are treated by neurologists at tertiary centers according to the place of residence. We prospectively followed-up pregnancies in women with epilepsy and healthy controls, and analyzed the factors responsible for their delivery outcomes and development of their babies. Healthy pregnant women had a higher level of education and economic status, but pregnant women with epilepsy took folic acid in a higher proportion than controls, possibly due to timely preconception counseling. Complications during pregnancy depended on the number of antiepileptic drugs and epilepsy control. We noticed some behavioral and cognitive aspects in children exposed in utero to valproic acid, which required follow up. The rate of congenital malformations was not increased. In conclusion, women with epilepsy should receive preconception counseling about the risk for pregnancy, but also about the possibilities to minimize that risk. We have introduced a model of integrative management of pregnancy and epilepsy based on close collaboration among different clinical experts in Croatia, in order to provide prompt counseling and timely intervention.Epilepsija je najčešća neurološka komplikacija u trudnoći. Žene s epilepsijom imaju veći rizik za komplikacije u trudnoći. U Hrvatskoj žene s epilepsijom obično prate neurolozi u tercijarnim centrima prema mjestu njihova boravka. Mi smo prospektivno pratili trudnoće u žena s epilepsijom i u zdravih kontrolnih trudnica te analizirali čimbenike odgovorne za ishode trudnoća i razvoj njihove djece. Zdrave trudnice su imale višu razinu obrazovanja i ekonomskog statusa, ali su žene s epilepsijom uzimale folnu kiselinu u višem postotku nego zdrave trudnice zahvaljujući pravodobnom predkoncepcijskom savjetovanju. Komplikacije za vrijeme trudnoće ovisile su o broju antiepileptičnih lijekova i kontroli epilepsije. Zamijetili smo određene bihevijoralne i kognitivne aspekte u djece izložene in utero valproičnoj kiselini, što zahtijeva daljnje praćenje. Stopa kongenitalnih malformacija nije bila povišena. U zaključku, ženama s epilepsijom trebali bismo omogućiti predkoncepcijsko savjetovanje o rizicima u trudnoći, ali i o mogućnostima kako značajno smanjiti taj rizik. Predstavili smo model integrativne prospektivne skrbi trudnica s epilepsijom koji se temelji na bliskoj suradnji različitih kliničkih eksperata u Hrvatskoj, u cilju osiguranja promptnog savjetovanja i pravodobne intervencije

Water for all : Proceedings of the 7th international scientific and professional conference Water for all

The 7th International Scientific and Professional Conference Water for all is organized to honour the World Water Day by the Josip Juraj Strossmayer University of Osijek, European Hygienic Engineering & Design Group (EHEDG), Danube Parks, Croatian Food Agency, Croatian Water, Faculty of Food Technology Osijek, Faculty of Agriculture in Osijek, Faculty of Civil Engineering Osijek, Josip Juraj Strossmayer University of Osijek Department of Biology, Josip Juraj Strossmayer University of Osijek Department of Chemistry, Nature Park “Kopački rit”, Osijek- Baranja County, Public Health Institute of the Osijek- Baranja County and „Vodovod-Osijek“ -water supply company in Osijek. The topic of World Water Day 2017 was "Wastewater" emphasizing the importance and influence of wastewater treatments on global environment. The international scientific and professional conference Water for all is a gathering of scientists and experts in the field of water management, including chemists, biologists, civil and agriculture engineers, with a goal to remind people about the significance of fresh water and to promote an interdisciplinary approach and sustainability for fresh water resource management. The Conference has been held since 2011. About 300 scientists and engineers submitted 95 abstracts to the 7th International Scientific and Professional Conference Water for all, out of which 33 was presented orally and 62 as posters. 47 full papers were accepted by the Scientific Committee. 38 full papers became the part of the this Proceedings while 9 papers were accepted for publication in Croatian Journal of Food Science and Technology and Electronic Journal of the Faculty of Civil Engineering Osijek - e-GFOS

Fonološka svesnost, fonemski sluh i artikulacione sposobnosti kod dece predškolskog uzrasta

Да би дете овладало говором, потребно је да постоји органска основа за развој говора, а то значи пре свега добар слух, нормална интелигенција, говорна моторика, добра аудитивна перцепција и дискриминација гласова и капацитет за развој језичких способности (Голубовић, 1998, 2006, 2012, 2016). Циљ овог истраживања је да се утврди развијеност фонолошке свесности, фонемског слуха, као и развијеност артикулационих способности код деце типичног развоја. Узорком је обухваћено 60-оро деце, узраста од 6 до 7 година. У истраживању су коришћени следећи мерни инструменти: Тест оралне праксије (Радичевић, & Стеванковић, 1992), Глобални артикулациони тест (Костић, Владисављевић, & Поповић, 1983), Тест за испитивање разликовања фонема (Костић, Владисављевић, & Поповић, 1983), Тест гласовне анализе и синтезе речи (Радичевић, 1991) и четири субтеста из Теста фонолошке свесности-ФОНТ теста (Суботић, 2011). Као методолошки оквир, коришћена је статистичка обрада података уз подршку софтверског пакета SPSS. Добијени резултати показују да је на узорку деце предшколског узраста утврђено 45% деце са неправилним изговором, при чему се најчешће неправилно изговарају гласови Ц, С, Р и Љ. Резултати истраживања фонемског слуха показују да деца у просеку тачно дискриминишу 39 од 40 фонемских парова, при чему се најчешће нетачно дискриминишу парови фонема: Ч-Џ; М-Б; Л-Р и С-З. На задацима анализе гласова у речима, утврђено је 71,7%-96,7% тачних одговора, док је на задацима синтезе гласова у речима утврђено 73,3%- 85% тачних одговора код деце из узорка. У оквиру задатака фонолошке свесности деца из узорка су остварила највећи успех на задацима слоговне свесности (висе од 95% тачних одговора), док је највећи проблем за децу представљао задатак индетификовања завршног фонема, са утврђених 76,7%-98,3% тачних одговора.To a child mastered speech, it is necessary that there is an organic basis for the development of speech, and that means first of all a good hearing, normal intelligence, speech motor, good auditory perception and capacity to develop language skills (Golubović, 1998, 2006, 2012, 2016). The aim of this research is to determine the development of phonological awereness, phonemic hearing, as well as the development of articulation abilities in children with typical development. The sample included 60 children, aged 6 to 7 years. In the study, were used the following measuring instruments: Test of oral praxis (Radičević, & Stevanković, 1992), The Global articulation test (Kostić, Vladisavljević & Popović, 1983), Test to examine the phoneme differentiation (Kostić, Vladisavljević & Popović, 1983), Test analysis and synthesis of voice to word (Radičević, 1991), and four subtests from the Phonological awareness test- FONT test (Subotić, 2011). As a methodological framework was used statistical processing with the support of the software package SPSS. The results obtained indicate that the sample pre-school children determined 45% of children with incorrect pronuncation, most of them incorrectly spoken voices C, S, R and LJ. Research results show that phonemic hearing children on average exactly discriminate against 39 of the 40 phonemic pairs, whereby the most often incorrectly discriminates against couples phonemes: C-DZ; M-B; L-R and S-Z. On tasks for voice analysis in the words, it has been found 71,7%-96,7% correct answers, while the tasks of vote in the synthesis of words determined to 73,3%-85% correct responses in children in the sample. In the context of task of phonological awereness of children in the sample were achieved greatest success in the task of syllable awereness (more than 95% correct answers), while the biggest problem for children respresented the task of identifying the final phonemes, determined to 76,7%-98,3% correct answers

Fonološka svesnost, fonemski sluh i artikulacione sposobnosti kod dece predškolskog uzrasta

Да би дете овладало говором, потребно је да постоји органска основа за развој говора, а то значи пре свега добар слух, нормална интелигенција, говорна моторика, добра аудитивна перцепција и дискриминација гласова и капацитет за развој језичких способности (Голубовић, 1998, 2006, 2012, 2016). Циљ овог истраживања је да се утврди развијеност фонолошке свесности, фонемског слуха, као и развијеност артикулационих способности код деце типичног развоја. Узорком је обухваћено 60-оро деце, узраста од 6 до 7 година. У истраживању су коришћени следећи мерни инструменти: Тест оралне праксије (Радичевић, & Стеванковић, 1992), Глобални артикулациони тест (Костић, Владисављевић, & Поповић, 1983), Тест за испитивање разликовања фонема (Костић, Владисављевић, & Поповић, 1983), Тест гласовне анализе и синтезе речи (Радичевић, 1991) и четири субтеста из Теста фонолошке свесности-ФОНТ теста (Суботић, 2011). Као методолошки оквир, коришћена је статистичка обрада података уз подршку софтверског пакета SPSS. Добијени резултати показују да је на узорку деце предшколског узраста утврђено 45% деце са неправилним изговором, при чему се најчешће неправилно изговарају гласови Ц, С, Р и Љ. Резултати истраживања фонемског слуха показују да деца у просеку тачно дискриминишу 39 од 40 фонемских парова, при чему се најчешће нетачно дискриминишу парови фонема: Ч-Џ; М-Б; Л-Р и С-З. На задацима анализе гласова у речима, утврђено је 71,7%-96,7% тачних одговора, док је на задацима синтезе гласова у речима утврђено 73,3%- 85% тачних одговора код деце из узорка. У оквиру задатака фонолошке свесности деца из узорка су остварила највећи успех на задацима слоговне свесности (висе од 95% тачних одговора), док је највећи проблем за децу представљао задатак индетификовања завршног фонема, са утврђених 76,7%-98,3% тачних одговора.To a child mastered speech, it is necessary that there is an organic basis for the development of speech, and that means first of all a good hearing, normal intelligence, speech motor, good auditory perception and capacity to develop language skills (Golubović, 1998, 2006, 2012, 2016). The aim of this research is to determine the development of phonological awereness, phonemic hearing, as well as the development of articulation abilities in children with typical development. The sample included 60 children, aged 6 to 7 years. In the study, were used the following measuring instruments: Test of oral praxis (Radičević, & Stevanković, 1992), The Global articulation test (Kostić, Vladisavljević & Popović, 1983), Test to examine the phoneme differentiation (Kostić, Vladisavljević & Popović, 1983), Test analysis and synthesis of voice to word (Radičević, 1991), and four subtests from the Phonological awareness test- FONT test (Subotić, 2011). As a methodological framework was used statistical processing with the support of the software package SPSS. The results obtained indicate that the sample pre-school children determined 45% of children with incorrect pronuncation, most of them incorrectly spoken voices C, S, R and LJ. Research results show that phonemic hearing children on average exactly discriminate against 39 of the 40 phonemic pairs, whereby the most often incorrectly discriminates against couples phonemes: C-DZ; M-B; L-R and S-Z. On tasks for voice analysis in the words, it has been found 71,7%-96,7% correct answers, while the tasks of vote in the synthesis of words determined to 73,3%-85% correct responses in children in the sample. In the context of task of phonological awereness of children in the sample were achieved greatest success in the task of syllable awereness (more than 95% correct answers), while the biggest problem for children respresented the task of identifying the final phonemes, determined to 76,7%-98,3% correct answers

Evaluation of high sensitivity C-reactive protein assay in cerebrospinal fluid on the Dimension RxL analyzer

<strong>Introduction</strong>: Low sensitivity and specificity in traditional laboratory tests became insufficient for accurate diagnostics and initiation of proper treatment of patients infected with bacterial meningitis. High sensitivity C reactive protein (hsCRP) may be an appropriate supplement for rapid diagnosis of bacterial meningitis. The subject of our investigation was the determination of C- reactive protein in cerebrospinal fluid (CSF) during<br />acute bacterial meningitis.<br /><strong>Methods:</strong> HsCRP was analysed by a sensitive immunoturbidimetric assay using the Dimension RxL analyser (Siemens). Cerebrospinal fluid concentrations of C-reactive protein have been measured in 20 patients<br />(age range,1 to 50 years) presenting with acute bacterial meningitis and also in a non-infected, non-inflamed control group (n=25).<br /><strong>Results:</strong> The accuracy and precision of the method proved to be satisfactory. Repeatability of serial sampling for hsCRP described by coefficient of variation were CV=2.1-4.5%. This assay hsCRP in cerebrospinal fluid demonstrates adequate performance characteristics for routine clinical use. Elevated levels of CRP were found in 95% patients with bacterial meningitis. The mean CRP value in 25 uninfected control group was 0.25 mg/L (range 0.10-0.55). The mean CRP for patients with bacterial meningitis was 21.4 mg/L (range 0.40-100).<br /><strong>Conclusions:</strong> A sensitive assay for CRP in CSF would be an useful adjunct to conventional investigation of acute infective meningitis