Luminescence lifetime.

<p>a) Long and b) short components of luminescence lifetime values measured at 612 nm for the ⁵D₀→⁷F₂ emission band in Eu³⁺ ions in hybrid systems consisting of 10% Eu³⁺ doped NaYF₄ NPs and DNA molecules bound to the NPs surface. The wavelength selection was performed with a JobinYvon THR1000 monochromator. Included numbers are the energy transfer efficiencies, η calculated based on the FRET mechanism.</p

Luminescence lifetime.

<p>a) Long and b) short components of luminescence lifetime values measured at 612 nm for the ⁵D₀→⁷F₂ emission band in Eu³⁺ ions in hybrid systems consisting of 10% Eu³⁺ doped NaYF₄ NPs and DNA molecules bound to the NPs surface. The wavelength selection was performed with a JobinYvon THR1000 monochromator. Included numbers are the energy transfer efficiencies, η calculated based on the FRET mechanism.</p

Luminescence spectra of NaYF₄:10%Eu³⁺ NPs-ssDNA-NH₂ complex excited with 394 nm laser light (red curve).

<p>The absorption (α_Cy5, grey shaded curve) and emission (I(λ)_Cy5, blue shaded curve) spectra under 650 nm excitation for Cy5 fluorophore are presented for comparison.</p

Luminescent properties of NaYF₄:10%Eu⁺³ NPs.

<p>a) emission spectrum of synthesized NaYF4:10%Eu⁺³ NPs in cyclohexane b) emission spectrum of NaYF₄:10%Eu⁺³ NPs after transfer to water—aqueous fraction c) emission spectrum of the organic fraction remaining after NPs transferred to water. All emission spectra were carried out with the excitation wavelength of 394 nm at room temperature.</p