Einfluss von drei Einzelnucleotidpolymorphismen im CAPN1 Gen auf die Zartheit von Rindfleisch

Meat tenderness is an important trait in beef cattle production, as consumers consider tenderness the most important attribute of beef palatability. There is ample evidence that post mortem proteolysis of myofibrillar proteins is responsible for the decline in shear force uring storage. The bovine micromolar calcium-activated neutral protease CAPN1) gene encodes the large subunit of μ-calpain, which is thought to be one of the most important enzymes involved in post mortem tenderization (KOOHMARAIE 1996). Three single-nucleotide polymorphisms (SNPs) on the CAPN1 gene (316, 530 and 4 751 markers)have been associated with tenderness in different cattle breeds (PAGE et al. 2002, PAGE et al. 2004, WHITE et al. 2005). A more recent study confirmed that markers 316 and 4 751 had an effect on beef tenderness (VAN EENENNAAM et al. 2007). The objective of this research was to determine the existence of polymorphisms and to assess the effect of the reported SNP in the bovine CAPN1 gene on tenderness from a sample of Angus and Brangus steers fattened on pasture.Fil: Soria, Liliana A.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Corva, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Sica, Andrea Branda. Instituto Nacional de Investigación Agropecuaria; UruguayFil: Schor, Alejandro. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Bovinos de Carne; ArgentinaFil: Melucci, Lilia Magdalena. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Villarreal, Edgardo L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; ArgentinaFil: Mezzadra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; ArgentinaFil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Miquel, Maria Cristina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentin

The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture

The objective of this paper was to determine the association of a SNP in the μ-calpain gene at position 316 with growth and quality of meat traits of steers grown on pasture. Fifty-nine Brangus and 20 Angus steers were genotyped for CAPN1 316. Warner Bratzler shear force was measured in l. lumborum samples after a 7-day aging period. A multivariate analysis of variance was performed, including shear force (WBSF), final weight (FW), average daily gain (ADG), backfat thickness (BFT), average monthly fat thickness gain (AMFTG), rib-eye area (REA), and beef rib-eye depth (RED) as dependent variables. The CAPN1 316 genotype was statistically significant. Univariate analyses were done with these variables. The marker genotype was statistically significant (p < 0.05) for WBSF (kg: CC: 4.41 ± 0.57; CG: 5.58 ± 0.20; GG: 6.29 ± 0.18), FW (kg: CC: 360.23 ± 14.71; CG: 381.34 ± 5.26; GG: 399.23 ± 4.68), and ADG (kg/d: CC: 0.675 ± 0.046; CG: 0.705 ± 0.016; GG: 0.765 ± 0.014) Shear force, final weight and average daily gain were significantly different according to the CAPN1 316 marker genotypes. The marker genotype was statistically significant in the multivariate analysis (p = 0.001). The first characteristic root explained 89% of the differences among genotypes. WBSF, FW and ADG were the most important traits in the first vector, indicating that animals with the marker genotype for lowest WBSF also have the lowest FW and ADG

Receptor-Induced Dilatation in the Systemic and Intrarenal Adaptation to Pregnancy in Rats

Normal pregnancy is associated with systemic and intrarenal vasodilatation resulting in an increased glomerular filtration rate. This adaptive response occurs in spite of elevated circulating levels of angiotensin II (Ang II). In the present study, we evaluated the potential mechanisms responsible for this adaptation. The reactivity of the mesangial cells (MCs) cultured from 14-day-pregnant rats to Ang II was measured through changes in the intracellular calcium concentration ([Cai]). The expression levels of inducible nitric oxide synthase (iNOS), the Ang II-induced vasodilatation receptor AT2, and the relaxin (LGR7) receptor were evaluated in cultured MCs and in the aorta, renal artery and kidney cortex by real time-PCR. The intrarenal distribution of LGR7 was further analyzed by immunohistochemistry. The MCs displayed a relative insensitivity to Ang II, which was paralleled by an impressive increase in the expression level of iNOS, AT2 and LGR7. These results suggest that the MCs also adapt to the pregnancy, thereby contributing to the maintenance of the glomerular surface area even in the presence of high levels of Ang II. The mRNA expression levels of AT2 and LGR7 also increased in the aorta, renal artery and kidney of the pregnant animals, whereas the expression of the AT1 did not significantly change. This further suggests a role of these vasodilatation-induced receptors in the systemic and intrarenal adaptation during pregnancy. LGR7 was localized in the glomeruli and on the apical membrane of the tubular cells, with stronger labeling in the kidneys of pregnant rats. These results suggest a role of iNOS, AT2, and LGR7 in the systemic vasodilatation and intrarenal adaptation to pregnancy and also suggest a pivotal role for relaxin in the tubular function during gestation

Claiming Greyness:Dutch Coloniality Against Polarisation

In this chapter we problematize the general assumption that appeals against polarization, which aim to return society to a state of balance and stability, are unquestionably good. We posit that, at closer inspection, such appeals help normalize racial violence through a politics of constraint and a post-political understanding of polarization. Through an analysis of the campaign "Dare to be Grey"'s rhetorical positioning, we demonstrate that rather than serving as a “neutral” alternative to “black/white thinking,” “greyness” actually reinforces the colonial logics of whiteness and anti-blackness

Determinación de la variabilidad animal como parámetro indicador de resistencia a la Mosca de los Cuernos (Haematobia irritans)

p.227-230Para responder al interrogante sobre la existencia de la variabilidad animal como parámetro de resistencia a la mosca de los cuernos (Haematobia irritans), se trabajó en dos establecimientos de la provincia de Buenos Aires: establecimiento A, ubicado en la localidad de Nueve de Julio con un lote de vaquillonas, n igual a 13, establecimiento B ubicado en Las Meras, con dos lotes: lote 1: vaquillonas, n igual a 19, lote 2: toros, n igual a 10. En ambos establecimientos se realizaron lecturas cada 15 días del conteo de moscas durante los meses de verano, ya que en esta estación se manifiesta el mayor ataque del parásito. El análisis de los datos obtenidos se realizó a través de un diseño completamente aleatorizado, y la comparación de medias mediante el Test de Tukey. Con respecto a los resultados tanto en el establecimiento A como en el B se encontraron diferencias significativas, en el rodeo de vaquillonas (p menor a 0,05), no ocurriendo lo mismo en el roedo de toros, (establecimiento B). Se concluye que existe variabilidad animal (al menos en vaquillonas), con respecto al número de moscas