Reconstruction stores in Vyskov - construction technology project

This master´s thesis deals with a complete renovation of a shop in Vyškov. A part of this thesis represents a technical report of the worksite facilities, technological standard, inspection and test plan, machine design, plan of safety and protection of environment, contract for work, budget and schedule

Additional file 4: of The host-pathogen interaction between wheat and yellow rust induces temporally coordinated waves of gene expression

Expression data (TPM values) for significantly differentially expressed genes was normalized and clustered into sets of genes with qualitatively similar expression profiles using the mini batch k-means algorithm, resulting in 8 clusters for PST. (EPS 18763 kb

Wheat Stripe Rust Resistance Protein WKS1 Reduces the Ability of the Thylakoid-Associated Ascorbate Peroxidase to Detoxify Reactive Oxygen Species.

Stripe rust is a devastating fungal disease of wheat caused by Puccinia striiformis f. sp tritici (Pst). The WHEAT KINASE START1 (WKS1) resistance gene has an unusual combination of serine/threonine kinase and START lipid binding domains and confers partial resistance to Pst. Here, we show that wheat (Triticum aestivum) plants transformed with the complete WKS1 (variant WKS1.1) are resistant to Pst, whereas those transformed with an alternative splice variant with a truncated START domain (WKS1.2) are susceptible. WKS1.1 and WKS1.2 preferentially bind to the same lipids (phosphatidic acid and phosphatidylinositol phosphates) but differ in their protein-protein interactions. WKS1.1 is targeted to the chloroplast where it phosphorylates the thylakoid-associated ascorbate peroxidase (tAPX) and reduces its ability to detoxify peroxides. Increased expression of WKS1.1 in transgenic wheat accelerates leaf senescence in the absence of Pst. Based on these results, we propose that the phosphorylation of tAPX by WKS1.1 reduces the ability of the cells to detoxify reactive oxygen species and contributes to cell death. This response takes several days longer than typical hypersensitive cell death responses, thus allowing the limited pathogen growth and restricted sporulation that is characteristic of the WKS1 partial resistance response to Pst

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Additional file 2. Install manual for Python environment, Anaconda Python distribution and OpenCV-Python binding