Intracellular Ca2+ release through ryanodine receptors contributes to AMPA receptor-mediated mitochondrial dysfunction and ER stress in oligodendrocytes

Overactivation of ionotropic glutamate receptors in oligodendrocytes induces cytosolic Ca2+ overload and excitotoxic death, a process that contributes to demyelination and multiple sclerosis. Excitotoxic insults cause well-characterized mitochondrial alterations and endoplasmic reticulum (ER) dysfunction, which is not fully understood. In this study, we analyzed the contribution of ER-Ca2+ release through ryanodine receptors (RyRs) and inositol triphosphate receptors (IP3Rs) to excitotoxicity in oligodendrocytes in vitro. First, we observed that oligodendrocytes express all previously characterized RyRs and IP3Rs. Blockade of Ca2+-induced Ca2+ release by TMB-8 following α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptor-mediated insults attenuated both oligodendrocyte death and cytosolic Ca2+ overload. In turn, RyR inhibition by ryanodine reduced as well the Ca2+ overload whereas IP3R inhibition was ineffective. Furthermore, AMPA-triggered mitochondrial membrane depolarization, oxidative stress and activation of caspase-3, which in all instances was diminished by RyR inhibition. In addition, we observed that AMPA induced an ER stress response as revealed by α subunit of the eukaryotic initiation factor 2α phosphorylation, overexpression of GRP chaperones and RyR-dependent cleavage of caspase-12. Finally, attenuating ER stress with salubrinal protected oligodendrocytes from AMPA excitotoxicity. Together, these results show that Ca2+ release through RyRs contributes to cytosolic Ca2+ overload, mitochondrial dysfunction, ER stress and cell death following AMPA receptor-mediated excitotoxicity in oligodendrocytes

Properties of bow-shock sources at the Galactic center

There are an enigmatic population of massive stars around the Galactic Center (GC) that were formed some Ma ago. A fraction of these stars has been found to orbit the supermassive black hole, SgrA*, in a projected clockwise disk, which suggests that they were formed in a formerly existing dense disk around SgrA*. We focus on the extended, near-infrared (NIR) sources IRS1W, IRS5, IRS10W, and IRS21 that have been suggested to be young, massive stars that form bow-shocks through their interaction with the ISM. Their nature has impeded accurate determination of their orbital parameters. We aim at establishing their nature and kinematics to test whether they form part of the clockwise disk. We performed NIR multi-wavelength imaging using adaptive optics (AO) and sparse aperture masking (SAM). We introduce a new method for self-calibration of the SAM PSF in dense stellar fields. The emission mechanism, morphology and kinematics of the targets were examined via 3D bow-shock models. We confirm previous findings that IRS21, IRS1W, and IRS5 are bow-shocks created by the interaction between mass-losing stars and the interstellar gas. The nature of IRS10W remains unclear. Our modeling shows that the bow-shock-emission is caused by thermal emission while the scattering of stellar light does not play any significant role. IRS 1W appears to be a bow-shock produced by an anisotropic stellar wind or by locally inhomogeneous ISM density. Our best-fit models provide an estimate of the local proper motion of the ISM in the NA in agreement with the published models. Assuming that all of the sources are tied to SgrA*, their orbital planes were obtained via a Monte-Carlo simulation. Our orbital analysis suggests that they are not part of any of the clockwise disk. We thus add more evidence to recent findings that a large part of the massive stars show apparently random orbital orientations.Comment: accepted for publication by A&A, 17 pages, 11 figures, 1 appendi

Unveiling the near-infrared structure of the massive-young stellar object NGC 3603 IRS 9A with sparse aperture masking and spectroastrometry

Contemporary theory holds that massive stars gather mass during their initial phases via accreting disk-like structures. However, conclusive evidence for disks has remained elusive for the most massive young objects. This is mainly due to significant observational challenges. Incisive studies, even targeting individual objects, are therefore relevant to the progression of the field. NGC 3603 IRS 9A* is a young massive stellar object still surrounded by an envelope of molecular gas. Previous mid-infrared observations with long-baseline interferometry provided evidence for a disk of 50 mas diameter at its core. This work aims at a comprehensive study of the physics and morphology of IRS 9A at near-infrared wavelengths. New sparse aperture masking interferometry data taken with NACO/VLT at Ks and Lp filters were obtained and analysed together with archival CRIRES spectra of the H2 and BrG lines. The calibrated visibilities recorded at Ks and Lp bands suggest the presence of a partially resolved compact object of 30 mas at the core of IRS 9A, together with the presence of over-resolved flux. The spectroastrometric signal of the H2 line shows that this spectral feature proceeds from the large scale extended emission (300 mas) of IRS 9A, while the BrG line appears to be formed at the core of the object (20 mas). This scenario is consistent with the brightness distribution of the source for near- and mid-infrared wavelengths at various spatial scales. However, our model suffers from remaining inconsistencies between SED modelling and the interferometric data. Moreover, the BrG spectroastrometric signal indicates that the core of IRS 9A exhibits some form of complexity such as asymmetries in the disk. Future high-resolution observations are required to confirm the disk/envelope model and to flesh out the details of the physical form of the inner regions of IRS 9A.Comment: Accepted to be published in Astronomy & Astrophysics, 13 pages, 14 figure

Direct Detection of the Tertiary Component in the Massive Multiple HD 150 136 with VLTI

Massive stars are of fundamental importance for almost all aspects of astrophysics, but there still exist large gaps in our understanding of their properties and formation because they are rare and therefore distant. It has been found that most O-stars are multiples. HD 150 136 is the nearest system to Earth with >100 M_sol, and provides a unique opportunity to study an extremely massive system. Recently, evidence for the existence of a third component in HD 150 136, in addition to the tight spectroscopic binary that forms the main component, was found in spectroscopic observations. Our aim was to image and obtain astrometric and photometric measurements of this component using long baseline optical interferometry to further constrain the nature of this component. We observed HD150136 with the near-infrared instrument AMBER attached to the ESO VLT Interferometer. The recovered closure phases are robust to systematic errors and provide unique information on the source asymmetry. Therefore, they are of crucial relevance for both image reconstruction and model fitting of the source structure. The third component in HD 150 136 is clearly detected in the high-quality data from AMBER. It is located at a projected angular distance of 7.3 mas, or about 13 AU at the line-of-sight distance of HD 150 136, at a position angle of 209 degrees East of North, and has a flux ratio of 0.25 with respect to the inner binary. We resolved the third component of HD 150 136 in J, H and K filters. The luminosity and color of the tertiary agrees with the predictions and shows that it is also an O main-sequence star. The small measured angular separation indicates that the tertiary may be approaching the periastron of its orbit. These results, only achievable with long baseline near infrared interferometry, constitute the first step towards the understanding of the massive star formation mechanisms

Colour Evolution of Rosé Wines after Bottling

This research reports on the colour evolution of six rosé wines during sixteen months of storage in the bottle.Colour changes were determined in terms of CIELAB colour parameters and in terms of the common colourcategories used in visual assessment. The colour measurement method reproduces the visual assessmentconditions during wine tasting with respect to wine sampler, illuminating source, observing background andsample-observer geometry. CIELAB L*, a*, b*, C* and hab colour coordinates were determined at seven differenttimes (t = 0, 20, 80, 153, 217, 300 and 473 days). The time evolution of colour coordinate values was studied usingmodels related to linear, quadratic and exponential rise to a maximum. Adjusted R2, average standard error andCIELAB ΔE* colour difference were used to compare models and evaluate their performance. For each colourcoordinate, the accuracy of model predictions was similar to the standard deviation associated with a singlemeasurement. An average ΔE* = 0.92 with a 90 percentile value ΔE*90% = 1.50 was obtained between measuredand predicted colour. These values are smaller than human colour discrimination thresholds. The classificationinto colour categories at different times depends on the wine sample. It was found that all wines take three tofour months to change from raspberry to strawberry colour and seven to eight months to reach the redcurrantcategory. Only two wines had developed salmon colour by the end of the experiment

Sub-milliarcsecond Imaging of Quasars and AGN

We have used the VLBA at 15 GHz to image the structure of 132 strong compact AGN and quasars with a resolution better than one milliarcsecond and a dynamic range typically exceeding 1000 to 1. These observations were made as part of a program to investigate the sub-parsec structure of quasars and AGN and to study the changes in their structure with time. Many of the sources included in our study, particularly those located south of +35 degrees, have not been previously imaged with milliarcsecond resolution. Each of the sources has been observed at multiple epochs. In this paper we show images of each of the 132 sources which we have observed. For each source we present data at the epoch which had the best quality data. The milliarcsecond jets generally appear one-sided but two-sided structure is often found in lower luminosity radio galaxies and in high luminosity quasars with gigahertz peaked spectra. Usually the structure is unresolved along the direction perpendicular to the jet, but a few sources have broad plumes. In some low luminosity radio galaxies, the structure appears more symmetric at 2 cm than at long wavelengths. The apparent long wavelength symmetry in these sources is probably due to absorption by intervening material. A few sources contain only a single component with any secondary feature at least a thousand times weaker. We find no obvious correlation of radio morphology and the detection of gamma-ray emission by EGRET.Comment: 19 pages, 3 tables, 3 figures. Figure 2 (132 contour diagrams) is long and is omitted here. Figure 2 may be viewed at http://www.cv.nrao.edu/2cmsurvey/ In press, Astronomical Journal, April 199

Serendipitous discovery of the long-sought AGN in Arp 299-A

Context: The dusty nuclear regions of luminous infra-red galaxies (LIRGs) are heated by either an intense burst of massive star formation, an active galactic nucleus (AGN), or a combination of both. Disentangling the contribution of each of those putative dust-heating agents is a challenging task, and direct imaging of the innermost few pc can only be accomplished at radio wavelengths, using very high-angular resolution observations. Aims: We observed the nucleus A of the interacting starburst galaxy Arp 299, using very long baseline interferometry (VLBI) radio observations at 1.7 and 5.0 GHz. Our aim was to characterize the compact sources in the innermost few pc region of Arp 299-A, as well as to detect recently exploded core-collapse supernovae. Methods: We used the European VLBI Network (EVN) to image the 1.7 and 5.0 GHz compact radio emission of the parsec-scale structure in the nucleus of Arp 299-A with milliarcsecond resolution. Results: Our EVN observations show that one of the compact VLBI sources, A1, previously detected at 5.0 GHz, has a flat spectrum between 1.7 and 5.0 GHz and is the brightest source at both frequencies. Our 1.7 GHz EVN image shows also diffuse, low-surface brightness emission extending westwards from A1 and displays a prominent core-jet structure. Conclusions: The morphology, radio luminosity, spectral index and ratio of radio-to-X-ray emission of the A1-A5 region is consistent with a low-luminosity AGN (LLAGN), and rules out the possibility that it is a chain of young radio supernovae (RSNe) and supernova remnants (SNRs). We therefore conclude that A1-A5 is the long-sought AGN in Arp 299-A. This finding may suggest that both starburst and AGN are frequently associated phenomena in mergers.Comment: Accepted for publication in Letters to Astronomy and Astrophysics on 12 August 2010. 4 pages, 1 figur

The population of SNe/SNRs in the starburst galaxy Arp 220. A self-consistent analysis of 20 years of VLBI monitoring

The nearby ultra-luminous infrared galaxy (ULIRG) Arp 220 is an excellent laboratory for studies of extreme astrophysical environments. For 20 years, Very Long Baseline Interferometry (VLBI) has been used to monitor a population of compact sources thought to be supernovae (SNe), supernova remnants (SNRs) and possibly active galactic nuclei (AGNs). Using new and archival VLBI data spanning 20 years, we obtain 23 high-resolution radio images of Arp 220 at wavelengths from 18 cm to 2 cm. From model-fitting to the images we obtain estimates of flux densities and sizes of all detected sources. We detect radio continuum emission from 97 compact sources and present flux densities and sizes for all analysed observation epochs. We find evidence for a LD-relation within Arp 220, with larger sources being less luminous. We find a compact source LF $n(L)\propto L^\beta$ with $\beta=-2.19\pm0.15$, similar to SNRs in normal galaxies. Based on simulations we argue that there are many relatively large and weak sources below our detection threshold. The observations can be explained by a mixed population of SNe and SNRs, where the former expand in a dense circumstellar medium (CSM) and the latter interact with the surrounding interstellar medium (ISM). Nine sources are likely luminous, type IIn SNe. This number of luminous SNe correspond to few percent of the total number of SNe in Arp 220 which is consistent with a total SN-rate of 4 yr$^{-1}$ as inferred from the total radio emission given a normal stellar initial mass function (IMF). Based on the fitted luminosity function, we argue that emission from all compact sources, also below our detection threshold, make up at most 20\% of the total radio emission at GHz frequencies.Comment: Accepted for publication in Astronomy and Astrophysic