Molecular studies on intraspecific diversity and phylogenetic position of Coniothyrium minitans

Simple sequence repeat (SSR)±PCR amplification using a microsatellite primer (GACA)% and ribosomal RNA gene sequencing were used to examine the intraspecific diversity in the mycoparasite Coniothyrium minitans based on 48 strains, representing eight colony types, from 17 countries world-wide. Coniothyrium cerealis, C. fuckelii and C. sporulosum were used for interspecific comparison. The SSR±PCR technique revealed a relatively low level of polymorphism within C. minitans but did allow some differentiation between strains. While there was no relationship between SSR±PCR profiles and colony type, there was some limited correlation between these profiles and country of origin. Sequences of the ITS 1 and ITS 2 regions and the 5±8S gene of rRNA genes were identical in all twenty-four strains of C. minitans examined irrespective of colony type and origin. These results indicate that C. minitans is genetically not very variable despite phenotypic differences. ITS and 5±8S rRNA gene sequence analyses showed that C. minitans had similarities of 94% with C. fuckelii and C. sporulosum (which were identical to each other) and only 64% with C. cerealis. Database searches failed to show any similarity with the ITS 1 sequence for C. minitans although the 5±8S rRNA gene and ITS 2 sequences revealed an 87% similarity with Aporospora terricola. The ITS sequence including the 5±8S rRNA gene sequence of Coniothyrium cerealis showed 91% similarity to Phaeosphaeria microscopica. Phylogenetic analyses using database information suggest that C. minitans, C. sporulosum, C. fuckelii and A. terricola cluster in one clade, grouping with Helminthosporium species and 'Leptosphaeria' bicolor. Coniothyrium cerealis grouped with Ampelomyces quisqualis and formed a major cluster with members of the Phaeosphaeriacae and Phaeosphaeria microscopica

INSURANCE-INSURABLE INTEREST-JOINT ADVENTURERS

Plaintiff and the deceased were operating an airplane commercially. Plaintiff had purchased the plane, and was paying the deceased $25 per week plus half of the profits of the venture. The deceased acted as pilot, and was instrumental in obtaining business. Plaintiff took out insurance on the plane, and also on the life of the deceased, although their only relationship was through the joint venture. The plane was wrecked and the deceased was killed while on company business. The claim for the plane was paid, but the defendant refused to pay on the life insurance policy, claiming that the plaintiff had no insurable interest in the life of the deceased. The case was submitted to a jury which found that the plaintiff had an insurable interest. Defendant appealed. Held, affirmed. It was not necessary to prove that the death of the insured resulted in a substantial loss to the beneficiary . . . . It is sufficient that the beneficiary has a reasonable expectation of some benefit or advantage from the continuance of the life of the assured. Indemnity Ins. Co. of North America v. Dow, (6th Cir. 1949) 174 F. (2d) 168

WILLS-HOLOGRAPHIC-EVIDENCE AS TO TESTAMENTARY INTENT

The deceased had in her possession, at the time of her death, an envelope entitled \u27Will of Ella McNair. The envelope contained three separate sheets of paper dated some nineteen months prior to Ella\u27s death, upon which was written, entirely in the hand of the deceased, what purported to be a will. The document opened with the statement I, Ella McNair . . . do hereby make my last will. The exordium was followed by fifteen specific bequests, and then the writing ended abruptly at the middle of the back of the third sheet. At the top of the second sheet was written \u27Will of Ella McNair, and \u27Will. Ella McNair was at the top of the third. Ella had, with a few exceptions, followed the form of a holographic will provided by her attorney. She had omitted the clause revoking prior wills, the clause appointing an executor, and her signature. There was evidence that before and during Ella\u27s final visit to the hospital, the instrument in question was not regarded by her as her will, and that she was planning to draw up a new one. The will was admitted to the probate, and the contestants appealed. Held, affirmed, two justices dissenting. Taking into account the general appearance of the writing, the language and phraseology employed therein, . . . we are constrained to hold that there was, at least in the mind of Mrs. McNair, a document before her sufficiently complete to induce her to affix her name thereon [referring to the inscription \u27Will. Ella McNair\u27 at the top of the third page] in token of execution of the same as her will. In re McNair\u27s Estate, (S.D. 1949) 38 N.W. (2d) 449

Role of ultrasound in the evaluation of first-trimester pregnancies in the acute setting

In patients presenting for an evaluation of pregnancy in the first trimester, transvaginal ultrasound is the modality of choice for establishing the presence of an intrauterine pregnancy; evaluating pregnancy viability, gestational age, and multiplicity; detecting pregnancy-related complications; and diagnosing ectopic pregnancy. In this pictorial review article, the sonographic appearance of a normal intrauterine gestation and the most common complications of pregnancy in the first trimester in the acute setting are discussed

Adjusting bone mass for differences in projected bone area and other confounding variables: an allometric perspective.

The traditional method of assessing bone mineral density (BMD; given by bone mineral content [BMC] divided by projected bone area [Ap], BMD = BMC/Ap) has come under strong criticism by various authors. Their criticism being that the projected bone "area" (Ap) will systematically underestimate the skeletal bone "volume" of taller subjects. To reduce the confounding effects of bone size, an alternative ratio has been proposed called bone mineral apparent density [BMAD = BMC/(Ap)3/2]. However, bone size is not the only confounding variable associated with BMC. Others include age, sex, body size, and maturation. To assess the dimensional relationship between BMC and projected bone area, independent of other confounding variables, we proposed and fitted a proportional allometric model to the BMC data of the L2-L4 vertebrae from a previously published study. The projected bone area exponents were greater than unity for both boys (1.43) and girls (1.02), but only the boy's fitted exponent was not different from that predicted by geometric similarity (1.5). Based on these exponents, it is not clear whether bone mass acquisition increases in proportion to the projected bone area (Ap) or an estimate of projected bone volume (Ap)3/2. However, by adopting the proposed methods, the analysis will automatically adjust BMC for differences in projected bone size and other confounding variables for the particular population being studied. Hence, the necessity to speculate as to the theoretical value of the exponent of Ap, although interesting, becomes redundant

Ecosystem carbon 7 dioxide fluxes after disturbance in forests of North America

Disturbances are important for renewal of North American forests. Here we summarize more than 180 site years of eddy covariance measurements of carbon dioxide flux made at forest chronosequences in North America. The disturbances included stand-replacing fire (Alaska, Arizona, Manitoba, and Saskatchewan) and harvest (British Columbia, Florida, New Brunswick, Oregon, Quebec, Saskatchewan, and Wisconsin) events, insect infestations (gypsy moth, forest tent caterpillar, and mountain pine beetle), Hurricane Wilma, and silvicultural thinning (Arizona, California, and New Brunswick). Net ecosystem production (NEP) showed a carbon loss from all ecosystems following a stand-replacing disturbance, becoming a carbon sink by 20 years for all ecosystems and by 10 years for most. Maximum carbon losses following disturbance (g C m−2y−1) ranged from 1270 in Florida to 200 in boreal ecosystems. Similarly, for forests less than 100 years old, maximum uptake (g C m−2y−1) was 1180 in Florida mangroves and 210 in boreal ecosystems. More temperate forests had intermediate fluxes. Boreal ecosystems were relatively time invariant after 20 years, whereas western ecosystems tended to increase in carbon gain over time. This was driven mostly by gross photosynthetic production (GPP) because total ecosystem respiration (ER) and heterotrophic respiration were relatively invariant with age. GPP/ER was as low as 0.2 immediately following stand-replacing disturbance reaching a constant value of 1.2 after 20 years. NEP following insect defoliations and silvicultural thinning showed lesser changes than stand-replacing events, with decreases in the year of disturbance followed by rapid recovery. NEP decreased in a mangrove ecosystem following Hurricane Wilma because of a decrease in GPP and an increase in ER

Physics on the edge: contour dynamics, waves and solitons in the quantum Hall effect

We present a theoretical study of the excitations on the edge of a two-dimensional electron system in a perpendicular magnetic field in terms of a contour dynamics formalism. In particular, we focus on edge excitations in the quantum Hall effect. Beyond the usual linear approximation, a non-linear analysis of the shape deformations of an incompressible droplet yields soliton solutions which correspond to shapes that propagate without distortion. A perturbative analysis is used and the results are compared to analogous systems, like vortex patches in ideal hydrodynamics. Under a local induction approximation we find that the contour dynamics is described by a non-linear partial differential equation for the curvature: the modified Korteweg-de Vries equation. PACS number(s): 73.40.Hm, 02.40.Ma, 03.40.Gc, 11.10.LmComment: 15 pages, 12 embedded figures, submitted to Phys. Rev.

Solitons on the edge of a two-dimensional electron system

We present a study of the excitations of the edge of a two-dimensional electron droplet in a magnetic field in terms of a contour dynamics formalism. We find that, beyond the usual linear approximation, the non-linear analysis yields soliton solutions which correspond to uniformly rotating shapes. These modes are found from a perturbative treatment of a non-linear eigenvalue problem, and as solutions to a modified Korteweg-de Vries equation resulting from a local induction approximation to the nonlocal contour dynamics. We discuss applications to the edge modes in the quantum Hall effect.Comment: 4 pages, 2 eps figures (included); to appear in Phys. Rev. Letter

Kidney and Pancreas Transplantation in the United States, 1997–2006: The HRSA Breakthrough Collaboratives and the 58 DSA Challenge

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74980/1/j.1600-6143.2008.02173.x.pd

Prenatal Exposure to Lead, δ-Aminolevulinic Acid, and Schizophrenia: Further Evidence

Background: A previously conducted study of prenatal lead exposure and schizophrenia using δ-aminolevulinic acid, a biologic marker of Pb exposure, in archived maternal serum samples collected from subjects enrolled in the Childhood Health and Development Study (1959–1966) based in Oakland, California, suggested a possible association between prenatal Pb exposure and the development of schizophrenia in later life. Objectives: In the present study we extend these findings using samples collected from the New England cohort of the National Collaborative Perinatal Project (1959–1966). Using similar methods, in this study we found results that suggest a comparable association in this cohort. Methods: We pooled matched sets of cases and controls from both the California and New England sites using a multilevel random-intercept logistic regression model, accounting for matching and site structure as well as adjusting for maternal age at delivery and maternal education. Results: The estimated odds ratio for schizophrenia associated with exposure corresponding to 15 μg/dL of blood Pb was 1.92 (95% confidence interval, 1.05–3.87; p = 0.03). Conclusion: Although several limitations constrain generalizability, these results are consistent with previous findings and provide further evidence for the role of early environmental exposures in the development of adult-onset psychiatric disorders