Study on porphyromonas gingivalis- and porphyromonas endodontalis- mediated signaling pathway

It is known that anaerobic microbes like Porphyromonas gingivalis (P. gingivalis) are involved in the progression and initiation of multiple forms of periodontal disease such as chronic periodontitis. However, in order to culture this gram-negative anaerobic microbes in the laboratory we need a long time (about 72 hours) with a complete absence of oxygen (≤0.001%) to reach optimal cell density. Therefore it is usually treated in an anaerobic chamber. Because this traditional method to culture P. gingivalis is costly and time consuming, we tried to elaborate in this study a new, rapid and less expensive method for culturing the anaerobes, such as P. gingivalis and P. endodontalis. With the use of a rotary shaker under anaerobic conditions, it would seal the bacterial growth media from oxygen exposure using a mineral oil overlay. Additional analysis by western blot helped us to study more about the cell’s biological functions. As a result, we found that the RAW264.7 cells treated by P. gingivalis or P. endodontalis showed three groups of kinase phosphorylation levels. As these kinases are key factors for the bacteria induced inflammation, we are interested to find how to reduce such bacteria (i.e. P. gingivalis and P. endodontalis) induced inflammation via regulation of these related kinase phosphorylation in future studies. Based on our experiment, we believe that this method can be suitable for growing most species of anaerobic bacteria. Thus, we envision that this new method could be widely used in areas of both research and industry in the near future

Evaluating Provider Knowledge on the Impact of Vitamin D Deficiency in Chronic Health Conditions

Vitamin D deficiency affects nearly one billion individuals globally. It is an essential vitamin to maintain bone health as well as the overall health of the body. Recent research reveals the significance of vitamin D deficiency in chronic health conditions like osteoporosis, depression, breast cancer, dementia, and viral infections like COVID-19. The Doctor of Nursing Practice (DNP) project aimed to evaluate if education regarding vitamin D deficiency was effective in improving provider knowledge, increases practice confidence levels, and results in self-reported practice changes. An educational session and two questionnaires were administered to healthcare providers in the state of Mississippi. Before the educational session, participants were asked to complete Questionnaire 1. Questionnaire 2 was to be completed immediately following the educational session. Each questionnaire was considered as independent samples and evaluation of the data was completed using descriptive and inferential statistics (p = \u3c.001). Practice confidence levels in assessing and managing vitamin D deficiency increased from 10.7% to 77.8% following the educational session. Further data revealed that 88.9% of the participants were very likely to implement changes in their current clinical practice because of the educational session. As a result, continued education for healthcare providers regarding screening, diagnosing, and treating vitamin D deficiency is recommended to improve healthcare outcomes of adult patient populations

PENGARUH KUALITAS PELAYANAN TERHADAP KEPUASAN PELANGGAN PADA TOKO BUKU GRAMEDIA BASUKI RAHMAT SURABAYA

Keberhasilan perusahaan sangat dipengaruhi oleh bagaimana perusahaan memuaskan pelanggan. Kepuasan pelanggan dipengaruhi oleh kualitas pelayanan yang diberikan perusahaan. Pelayanan yang berkualitas merupakan aspek yang dapat membedakan dan membuat unggul suatu perusahaan dengan para pesaingnya. Pelanggan yang puas akan melakukan pembelian ulang sehingga akan berpengaruh pada pendapatan perusahaan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh kualitas pelayanan yang terdiri dari tangible, reliability, responsiveness, assurance, dan empaty terhadap kepuasan pelanggan. Populasi dalam penelitian ini adalah semua pembeli di Toko Buku Gramedia Basuki Rahmat Surabaya yang jumlahnya tidak diketahui dengan pasti (infinite) sedangkan sampel yang diambil sebanyak 60 pembeli. Metode pengumpulan dilakukan dengan cara penyebaran kuesioner. Pengujian data menggunakan analisis regresi linier berganda dengan pengujian hipotesis menggunakan uji t. Berdasarkan hasil penelitian dapat diketahui bahwa tangible berpengaruh signifikan terhadap kepuasan pelanggan. Reliability berpengaruh signifikan terhadap kepuasan pelanggan. Responsiveness berpengaruh signifikan terhadap kepuasan pelanggan. Assurance berpengaruh signifikan terhadap kepuasan pelanggan. Empaty berpengaruh signifikan terhadap kepuasan pelanggan. Kata kunci : tangible, reliability, responsiveness, assurance, empaty, kepuasan pelangga

Evaluation of Light-curing Intensity Output and Students’ Knowledge among Dental Schools in Riyadh City

AIM: The aim of this study is interpreting the dental student’s knowledge of light-curing units from different universities and colleges in Riyadh city and to evaluate the intensity of the light output of light-emitting diode (LED)-curing devices in dental school clinics in Riyadh, Saudi Arabia. METHODS: One hundred eighty-two visible light-curing (VLC) units were randomly selected to have their light intensity output evaluated using Demetron® L.E.D. Radiometer – Kerr among dental school clinics in Riyadh city. The university hospitals we visited are King Khalid University Hospital, King Saud bin Abdulaziz University Hospital, Dar Al Uloom University Hospital, Alfarabi colleges’ dental clinics, and Princess Nourah Bint Abdulrahman University Hospital. To evaluate the knowledge of dental students about light intensity output, a questionnaire was given to the students. RESULTS: Out of the VLC units, 22 units (12%) measured inadequate intensity, 91 units (50%) measured marginal intensity, and 69 (37%) measured adequate intensity. The statistical significance was up to p < 0.05, p = 0. Most students and interns did not think that light-curing unit intensity influenced the tooth pulp (55.8%) and also reported not know the minimum wavelength of light cure intensity (62%). CONCLUSION: A significant difference was found between the light cure intensities in universities. As for the students’ knowledge, the research revealed poor insight toward basic concepts of VLC units and its maintenance

Effect of Grafting Nano-TiO2 on Sansevieria cylindrica Fiber Properties

Natural fibers, which are abundant, environmentally friendly, and biodegradable, are used as a replacement for synthetic fibers. The composite strength can be increased by treating the surfaces of natural fibers with suitable chemicals, which can also improve the interface interaction between fiber and matrix. Application of a coupling agent in chemical treatment is utilized to reinforce the bonding between fiber and matrix. The objective of the study is to determine the influence of silane concentration on the Sansevieria cylindrica fiber properties. The methods included fibers treatment using ethanol and coupling agent as dissolving and TiO2 with concentrations of 0%, 0.25%, 0.5%, 0.75%, and 1%. The mechanical strength testing was conducted through a single fiber test. Fiber morphology was observed using an electron microscope. FTIR analyzes the change in fiber chemical composition caused by TiO2 addition. As a result, the morphology of S. cylindrica fibers became rougher and showed a rougher surface after a silane concentration of 1%, but with the proper concentration, some fiber surfaces provided a good interface. Ti-O bonds are formed at a wavelength of 475 cm-1. The shift in a peak at 400–500 cm-1 indicates Ti-O-Ti group stretching vibrations believed to have originated from TiO2 particles. The mechanical strength increases as the concentration of TiO2 increases, with the highest fiber strength of 284.66 MPa observed at a TiO2 concentration of 1%. This represents a 26% higher tensile strength compared to the control specimen

Perception of Healthcare Facility Garden and Impressions of Interactive Elements

Perception of Healthcare Facility Garden and Impressions of Interactive Elements AbstractThis research presents the effects of indoor healing gardens and interactive elements (technology) on people in medical facilities: patients, visitors, and staff. Previous studies showed that medical facilities generally received a negative perception by people who visited or worked there; yet the effect of indoor greenery seemed to improve the perceptions of those surveyed. In this study, the potential of added technology to a garden brought a new aspect to be explored. The participants were shown a video of four simulations of indoor gardens with added interactive elements (e.g., lights, sounds, music, images, interactive floor tiles); afterwards, the participants completed a short survey. The results of the research corroborated the literature regarding the benefits of indoor gardens on the study participants’ enhanced mood when in a medical setting with the added appreciation for interactive elements. Of the interactive elements provided, the wall projections of flying birds had a positive feedback of 91.3 %. Interactive floor tiles had a high likability rate as well (85.1%). While many appreciated the garden-only option, participants favored the playful components as entertaining for the brief time they would be in the space awaiting an appointment or on a staff break. Enhancing all medical spaces with natural and interactive elements should be a design goal for future new builds and renovation projects in healthcare or other commercial buildings.Key-words: indoor garden healthcare, interactive elements (lights, audio-visual images, of birds and animals, sound, color), healthcare facility

4-[5-(Furan-2-yl)-3-trifluoro­methyl-1H-pyrazol-1-yl]benzene­sulfonamide

In the title compound, C14H10F3N3O3S, there are significant twists in the mol­ecule, as seen in the values of the dihedral angles between the pyrazole ring and each of the furan [31.1 (2)°] and benzene rings [55.58 (10)°]. The amino N atom occupies a position almost normal to the benzene ring [N—S—Car—Car (ar = aromatic) torsion angle = 83.70 (19)°]. One amino H atom forms a hydrogen bond to the tricoordinate pyrazole N atom and the other inter­acts with a sulfonamide O atom, forming a supra­molecular chain along [010]. The chains are consolidated into a supra­molecular layers via C—H⋯O inter­actions involving the second sulfonamide O atom; layers stack along [10-1]. The furan ring was found to be disordered over two diagonally opposite orientations of equal occupancy

Incidence, Bacterial causes and Antibiotic Resistance Patterns of Urinary Tract Infection in Pet Animals

T The primary goal of the study was to determine the prevalence and various bacterial risks of lower urinary tract infections (UTI) in diseased and seemingly healthy pet animals with and without urine retention whether they were catheterized or not. The bacterial isolates were in vitro tested for their antibiotic resistance and antibiotic resistance genes were investigated. Between October 2020 and January 2022, 128 urine samples were randomly collected from pets recruited to veterinary hospitals and clinics in Cairo and Giza. Samples were cultivated for bacteriological isolation. Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Klebsiella spp. were found to be the most common bacterial causes of urinary tract infections in pets, with prevalence rates of 32.9%, 28%, and 19.5%, respectively followed by Proteus mirabilis (P. mirabilis) and Pseudomonas aeruginosa (P. aeruginosa) with incidences of 18.2% and 1.2%, respectively. Based on bacterial types and their virulence genes, antibiotic resistance and multi-drug resistance (MDR) behaviour varied. Epidemiology, diagnosis, and control of the urinary tract infection would benefit from the identification and characterization of isolated bacterial species

4-(4-Bromo­phen­yl)-2-oxo-1,2,5,6-tetra­hydro­benzo[h]quinoline-3-carbonitrile

In the mol­ecule of the title compound, C20H13BrN2O, the tetra­hydro­benzo[h]quinoline fused-ring system is buckled owing to the ethyl­ene –CH2CH2– fragment, the benzene ring and the pyridine ring being twisted by 17.7 (1)°. The 4-substituted aromatic ring is bent away from the pyridine ring by 82.3 (1)° in order to avoid crowding the cyanide substituent. Two mol­ecules are linked by a pair of N—H⋯O hydrogen bonds to form a centrosymmetric dimer