MERCURY PHYTOTOXICITY IN COMMON BEANS IN SOILS OF MINAS GERAIS, BRAZIL

INTRODUCTION: Problems caused by contaminated areas have been increasingly found around the world. Mercury (Hg) is a trace element potentially toxic to humans and the environment. Thus, monitoring Hg levels in the environment has great importance. The transfer of trace elements from soils to plants is a complex process, that depends on soil attributes such as pH, cation exchange capacity, texture, and mineralogy and also plant attributes, such as root system lenght and others. In Brazil, agricultural areas with levels of Hg higher than 12 mg kg-1 are considered contaminated, since such levels of Hg are likely to come from non-natural sources. The State of Minas Gerais is the third largest producer of common beans in Brazil (2015/2016 farming year). The area planted with common beans is estimated in 339,000 hectares. The soil most used for such crop in the State of Minas Gerais is classified as Latosol (Oxisol). The plants of Phaseolus vulgaris L. cv. BRSMG Madrepérola have indeterminate growth habit, low tolerance to lodging, and can be classified as an early-season variety. This variety has high yield potential and resistance to diseases, such as the common mosaic virose and anthracnose. P. vulgaris is one of the preferred plant species for using in ecotoxicological tests to evaluate the potential effect of toxic substances. The objective of this work was to evaluate the emergence and early development of common bean variety BRSMG Madrepérola under doses of mercury chloride (HgCl2), in two Latosols (Oxisols)

MERCURY PHYTOTOXICITY IN COMMON BEANS IN SOILS OF MINAS GERAIS, BRAZIL

INTRODUCTION: Problems caused by contaminated areas have been increasingly found around the world. Mercury (Hg) is a trace element potentially toxic to humans and the environment. Thus, monitoring Hg levels in the environment has great importance. The transfer of trace elements from soils to plants is a complex process, that depends on soil attributes such as pH, cation exchange capacity, texture, and mineralogy and also plant attributes, such as root system lenght and others. In Brazil, agricultural areas with levels of Hg higher than 12 mg kg-1 are considered contaminated, since such levels of Hg are likely to come from non-natural sources. The State of Minas Gerais is the third largest producer of common beans in Brazil (2015/2016 farming year). The area planted with common beans is estimated in 339,000 hectares. The soil most used for such crop in the State of Minas Gerais is classified as Latosol (Oxisol). The plants of Phaseolus vulgaris L. cv. BRSMG Madrepérola have indeterminate growth habit, low tolerance to lodging, and can be classified as an early-season variety. This variety has high yield potential and resistance to diseases, such as the common mosaic virose and anthracnose. P. vulgaris is one of the preferred plant species for using in ecotoxicological tests to evaluate the potential effect of toxic substances. The objective of this work was to evaluate the emergence and early development of common bean variety BRSMG Madrepérola under doses of mercury chloride (HgCl2), in two Latosols (Oxisols)

Network analysis of SRC-1 reveals a novel transcription factor hub which regulates endocrine resistant breast cancer.

Steroid receptor coactivator 1 (SRC-1) interacts with nuclear receptors and other transcription factors (TFs) to initiate transcriptional networks and regulate downstream genes which enable the cancer cell to evade therapy and metastasise. Here we took a top-down discovery approach to map out the SRC-1 transcriptional network in endocrine resistant breast cancer. First, rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) was employed to uncover new SRC-1 TF partners. Next, RNA sequencing (RNAseq) was undertaken to investigate SRC-1 TF target genes. Molecular and patient-derived xenograft studies confirmed STAT1 as a new SRC-1 TF partner, important in the regulation of a cadre of four SRC-1 transcription targets, NFIA, SMAD2, E2F7 and ASCL1. Extended network analysis identified a downstream 79 gene network, the clinical relevance of which was investigated in RNAseq studies from matched primary and local-recurrence tumours from endocrine resistant patients. We propose that SRC-1 can partner with STAT1 independently of the estrogen receptor to initiate a transcriptional cascade and control regulation of key endocrine resistant genes

Enfrentamento da violência contra a mulher: articulação intersetorial e atenção integral

Trata-se de um estudo descritivo-exploratório, de abordagem qualitativa e com objetivo de identificar elementos que interferem no processo de enfrentamento da violência contra a mulher. Realizou-se entrevista com representantes de 15 serviços que integram a Rede de Atenção a Pessoas em Situação de Violência na cidade de Salvador, Bahia, Brasil. Os dados foram organizados com base na análise temática de Bardin. O estudo mostrou que a articulação intersetorial e a atenção disponibilizada pelos serviços são elementos que interferem no enfrentamento da violência contra a mulher. Ambos os elementos guardam relação com o fortalecimento da rede de atenção a mulheres vítimas de violência. O estudo sinaliza para a necessidade de se conhecer as atribuições de outras instituições e reafirma a importância da articulação entre elas. Oferece ainda elementos que orientam o desenvolvimento de políticas e ações articuladas no sentido de favorecer o processo de enfrentamento da violência contra as mulheres

Multiple Relationships of Nursing Care: the Emergence of Care "of the us"

The aim of this qualitative study was to comprehend the relationships of the care of the self, of care of the other, and of care "of the us" in the different dimensions of care, through an educational/reflexive/interpretative process with nursing professionals in a University Hospital, using the complexity perspective. The data were collected through workshops and submitted to content analysis. The following categories emerged: reflecting upon the meaning of care of the self, care of the other, and "of the us" for the "I - human being", and for the "I - nursing professional"; and reflecting and (re)constructing the meanings of the relationships of care for the self, care for the other, and care "for the us". The care "for the us" is an emerging theme, in construction, and impels a concern for the collective, as well as remits to the comprehension of the multiple and unending phenomenon of constant movement among the beings and between them and their environment, modifying, altering, and causing to be altered the networks of existent relationships.Se trata de un estudio de abordaje cualitativo con el objetivo de comprender las relaciones de cuidado de sí, del otro y "de nosotros" en las diferentes dimensiones del cuidado, a través de un proceso educativo/reflexivo/interpretativo con profesionales de enfermería de un Hospital Escuela, bajo la perspectiva de la complejidad. Los datos fueron recolectados mediante talleres y sometidos al análisis de contenido. Surgieron las categorías: reflexionado sobre el significado del cuidado de sí, del otro, y "de nosotros" para el "yo - ser humano" y para el "yo - profesional de enfermería"; y, reflexionando y (re)construyendo los significados de las relaciones del cuidado de sí, del otro y "de nosotros". El cuidado "de nosotros" es un tema nuevo, en construcción, incentiva la preocupación con el colectivo y se relaciona con la comprensión de los fenómenos múltiples e inagotables del constante movimiento entre los seres y de estos con su ambiente, modificando, alterando y haciendo alterar las redes de relaciones existentes.Este é estudo de abordagem qualitativa, com o objetivo de compreender as relações de cuidado de si, do outro e "do nós", nas diferentes dimensões do cuidado, através de um processo educativo/reflexivo/interpretativo com profissionais de enfermagem de um hospital escola, sob a perspectiva da complexidade. Os dados foram coletados mediante oficinas e submetidos à análise de conteúdo. Emergiram as categorias: refletindo o significado do cuidado de si, do outro e "do nós" para o "eu - ser humano" e para o "eu - profissional de enfermagem" e, refletindo e (re)construindo os significados das relações do cuidado de si, do outro e "do nós". O cuidado "do nós" é tema emergente, em construção, impele a preocupação com o coletivo e remete à compreensão dos fenômenos múltiplos e inesgotáveis do constante movimento entre os seres e, desses, com seu ambiente, modificando, alterando e fazendo alterar as redes de relações existentes

Analog-Digital Level Locked Loop

This work deals with the conception of an analog to digital loop locked on the input signal's value. This loop allows the quantification of a signal with a variable number of elementary cycles. Its application to analog to digital conversion constitutes a first step towards the creation of new analog to digital conversion architectures. The loop uses a local quantification scheme to take advantage of real signals inter-sample redundancy. Five quantification algorithms are presented. This allows the average number of conversion cycles per sample to be reduced with respect to the Successive Approximation Register (SAR) converter, for an equivalent electronic complexity. This gain in terms of average number of cycles per sample is converted into real benefits by the mean of three proposed conversion architectures. The first architecture is designed for low power and leads to consumption gains from 47% to 87% with respect to SAR converters. The second architecture is designed for speed and leads to an average number of cycles per sample as small as 1.06, which is comparable to a Flash converter. This speed is obtained thanks to 2 comparators only with respect to 255 comparators in the case of the Flash converter. The third architecture allows the creation of a continuous time bound of the signal value thanks to asynchronous logic. This representation allows the signal reconstruction at a better precision both in time and in dynamic.Ce travail présente la conception d'une boucle analogique numérique verrouillée sur l'amplitude du signal d'entrée. Cette boucle permet la quantification d'un échantillon en un nombre variable de cycles élémentaires. Son application à la conversion analogique numérique constitue un premier pas vers la création de nouvelles architectures de convertisseurs. La boucle repose sur une recherche locale du signal pour exploiter la redondance inter-échantillons des signaux réels. Cinq algorithmes de quantification sont présentés. Ce principe permet une réduction du nombre moyen de cycles de quantification par échantillon par rapport aux Convertisseurs à Approximations Successives (CAS), pour une complexité électronique équivalente. Ce gain en termes de nombre moyen de cycles par échantillon est converti en économie d'énergie ou en gain de vitesse par le biais de trois architectures de conversion proposées. La première architecture est conçue pour la basse consommation et conduit à des gains de consommation de 47% à 87% par rapport au CAS. La deuxième est conçue pour la vitesse. Les résultats montrent un nombre moyen de cycles de conversion par échantillon proche de 1 pour deux des trois signaux de test. Ces résultats sont comparables aux performances d'un convertisseur Flash et sont obtenus avec 2 comparateurs seulement par rapport aux 255 comparateurs d'un Flash équivalent. La troisième architecture est conçue pour la précision temporelle et dynamique. Elle repose sur la logique asynchrone et permet la construction d'une borne à temps continu du signal