Effect of Season on some Biochemical Components of The Ovarian Follicular Fluid in Local Iraqi Ewes

أجريت التجربة في الكلية االتقنية المسيب / مختبر الفسلجة التابع لقسم تقنيات الانتاج الحيواني لقياس الصفات الكيموحيوية في مبايض الاغنام المحلية العراقية وجمعت لهذه الدراسة (50) جهاز تناسلي أنثوي للأغنام المحلية من مجزرة كربلاء والنجف  لموسمين: الموسم الاول (أب) للعام2021 والموسم الثاني (كانون الثاني) للعام2022. أظهرت النتائج وجود زيادة معنوية  P≤0.01)) للبروتين والكولوكوز والكولسترول والصوديوم ومعنوية P≤0.05))  للكالسيوم في الموسم الاول مقارنة بالموسم الثاني, وكانت الزيادة عالية المعنوية P≤0.05))  للبوتاسيوم في الموسم الثاني مقارنة بالموسم الاول .اما بالنسبة للمقارنة بين للفئات الحجمية وجود فروقات معنوية عالية في حجم الجريبات للفئة الاولى عند مستوى معنوية(P≤0.01) اذ بلغ في الموسم الاول 8 (32.00%) وفي الموسم الثاني 13 (52.00%) وأيضا وجد فروقات عالية المعنوية في حجم الجريبات للفئة الثانية اذ بلغ في الموسم الاول  11 (44.00%) وفي الموسم الثاني 6 (24.00%)  عند نفس مستوى المعنوية اما في الجريبات الفئة الثالثة  فلم تكن هناك فروقات معنوية اذ بلغ في الموسم الاول 6 (24.00%) وفي الموسم الثاني 6 (24.00%).كما أختلفت الفئات معنويا ضمن الموسم الواحد، فقد كانت الزيادة عالية المعنوية للفئة الثانية P≤0.01)) مقارنة ببقية الفئات ضمن الموسم الاول,اما في الموسم الثاني فقد كانت الزيادة عالية المعنوية P≤0.01)) للفئة الاولى مقارنة ببقية الفئات.This study was conducted in the technical college of Mussaib / department of Animal Production Techniques to investigate the effect of season on some follicular fluid biochemical parameters in the ovaries of the- . Fifty ovaries were collected from Karbala and Najaf butcheries for two seasons: the first season (August 2021) and the second season (January 2022). Moreover, collected follicles were divided according to their sizes into three groups (first group < 3 mm), (second group 3-5 mm), and (third group > 5mm) The results showed a significant increase (P ≤ 0.01) for protein, glucose, cholesterol, and sodium, and a significant increase (P ≤ 0.05) for calcium in the first season compared to the second season, and the significant increase was (P ≤ 0.05) for potassium in the second season compared to the first season. As for the follicles sizes the result exhibited, there were highly significant differences in the size of the follicles for the first group (< 3 mm ) P≤0.01, as it reached in the first season 8 (32.00%) and in the second season 13 (52.00%) and also found highly significant differences in the size of the follicles for the second group (3-5 mm) 11 (44.00%) in the first season and 6 (24.00%) in the second season (P≤0.01). for the follicles of the third group (> 5mm), there were no significant differences, as it reached 6 (24.00%) in the first season and 6 (24.00%) in the second season. The groups also differed significantly within the same season. The highly significant increase for the second group was (P≤0.01) compared to the rest of the groups within the first season, while in the second season, the high significant increase was for the first group (P≤0.01) compared to the rest of the groups

New Techniques of Weighted Sum Method for Solving Multi-Objective Geometric Programming Problems

أجريت هذه الدراسة في الكلية التقنية بالمسيب / قسم تقنيات الإنتاج الحيواني لبحث تأثير الموسم على بعض المتغيرات البيوكيميائية للسوائل الجريبية في مبايض النعاج العراقية المحلية. جمعت خمسون مبيض من جزار كربلاء والنجف لموسمين: الموسم الأول (آب 2021) والموسم الثاني (كانون الثاني 2022). علاوة على ذلك ، تم تقسيم البصيلات المجمعة حسب أحجامها إلى ثلاث مجموعات (المجموعة الأولى <3 مم)، (المجموعة الثانية 3-5 مم)، (المجموعة الثالثة> 5 مم) وأظهرت النتائج زيادة معنوية (P 0.01) للبروتين. الجلوكوز والكوليسترول والصوديوم، وزيادة معنوية (P 0.05) للكالسيوم في الموسم الأول مقارنة بالموسم الثاني ، وكانت الزيادة المعنوية (P ≤ 0.05) للبوتاسيوم في الموسم الثاني مقارنة بالموسم الأول . أما بالنسبة لأحجام البصيلات التي أظهرتها النتيجة فقد كانت هناك فروق ذات دلالة إحصائية كبيرة في حجم البصيلات للمجموعة الأولى (أقل من 3 مم) P≤0.01 حيث وصلت في الموسم الأول 8 (32.00٪) وفي الموسم الثاني. 13 (52.00٪) ووجدوا أيضا فروق ذات دلالة إحصائية عالية في حجم البصيلات للمجموعة الثانية (3-5 ملم) 11 (44.00٪) في الموسم الأول و 6 (24.00٪) في الموسم الثاني ( (P≤0.01 أما بالنسبة لبصيلات المجموعة الثالثة (> 5 مم) فلم توجد فروق ذات دلالة إحصائية حيث بلغت 6 (24.00٪) في الموسم الأول و 6 (24.00٪) في الموسم الثاني. كما اختلفت المجموعات بشكل كبير خلال نفس الموسم. كانت الزيادة ذات المعنوية العالية للمجموعة الثانية (P≤0.01) مقارنة بباقي المجموعات خلال الموسم الأول، بينما في الموسم الثاني كانت الزيادة العالية ذات المعنوية العالية للمجموعة الأولى (P≤0.01) مقارنة بباقي المجموعات. من المجموعات.This study was conducted in the technical college of Mussaib / department of Animal Production Techniques to investigate the effect of season on some follicular fluid biochemical parameters in the ovaries of the-. Fifty ovaries were collected from Karbala and Najaf butcheries for two seasons: the first season (August 2021) and the second season (January 2022). Moreover, collected follicles were divided according to their sizes into three groups (first group < 3 mm), (second group 3-5 mm), and (third group > 5mm) The results showed a significant increase (P ≤ 0.01) for protein, glucose, cholesterol, and sodium, and a significant increase (P ≤ 0.05) for calcium in the first season compared to the second season, and the significant increase was (P ≤ 0.05) for potassium in the second season compared to the first season. As for the follicles sizes the result exhibited, there were highly significant differences in the size of the follicles for the first group (< 3 mm) P≤0.01, as it reached in the first season 8 (32.00%) and in the second season 13 (52.00%) and also found highly significant differences in the size of the follicles for the second group (3-5 mm) 11 (44.00%) in the first season and 6 (24.00%) in the second season (P≤0.01). for the follicles of the third group (> 5mm), there were no significant differences, as it reached 6 (24.00%) in the first season and 6 (24.00%) in the second season. The groups also differed significantly within the same season. The highly significant increase for the second group was (P≤0.01) compared to the rest of the groups within the first season, while in the second season, the high significant increase was for the first group (P≤0.01) compared to the rest of the group

Detection of some virulence factors with Biochemical investigation of Listeria monocytogenes associated with human infections.

This study was aimed to detect different virulence genes that might be present in the Listeria monocytogenes. 50 clinical samples were gathered from patients with different sites like (stool, urine and blood). Though, only (22) isolates were contained L.monocytogenes using conventional and traditional techniques. Strains were isolated with the following approach 8 (36%), 6 (27%), and 8 (36%) from human intestine (stool), urine, and blood samples respectively

Correlation of Metabolic and Ionic Components of Follicular Fluid with Estrogen Concentration

This study was aimed to assess the correlation between the metabolic and ionic composition of ovarian follicular fluid and its relationship to the concentration of estrogen and prolactin hormones as the follicle size changes in the local female cows. Ovaries were collected (80 ovaries) from non- pregnant 40 adult females cow at Hilla slaughterhouse at Babil province. The gathered ovaries were transported to the laboratory within two hours. Follicular fluid was pulled from small (2-5 mm) follicles, medium (6-10 mm), and large (11-20 mm) follicles, then it stored at 5 ° C until assayed. Follicular fluid samples were analyzed for metabolites composition of (cholesterol, total protein, and glucose), and ionic composition (calcium, phosphorus, copper, magnesium, zinc) ,and hormone concentration ( estrogen and prolactin). The results of the study showed that the total protein and glucose concentrations were increased significantly(p> 05.0) with increased of follicle size. The study also showed the calcium, copper, and phosphorus concentration were increased significantly(p> 05.0) with changes of follicular size, as well as the estrogen concentration was significantly increased (p > 05.0) With increased follicular size. A significantly (p> 05.0) correlation was found between both magnesium, zinc ions, and estrogen hormone

Correlation of Metabolic and Ionic Components of Follicular Fluid with Estrogen Concentration

This study was aimed to assess the correlation between the metabolic and ionic composition of ovarian follicular fluid and its relationship to the concentration of estrogen and prolactin hormones as the follicle size changes in the local female cows. Ovaries were collected (80 ovaries) from non- pregnant 40 adult females cow at Hilla slaughterhouse at Babil province. The gathered ovaries were transported to the laboratory within two hours. Follicular fluid was pulled from small (2-5 mm) follicles, medium (6-10 mm), and large (11-20 mm) follicles, then it stored at 5 ° C until assayed. Follicular fluid samples were analyzed for metabolites composition of (cholesterol, total protein, and glucose), and ionic composition (calcium, phosphorus, copper, magnesium, zinc) ,and hormone concentration ( estrogen and prolactin). The results of the study showed that the total protein and glucose concentrations were increased significantly(p> 05.0) with increased of follicle size. The study also showed the calcium, copper, and phosphorus concentration were increased significantly(p> 05.0) with changes of follicular size, as well as the estrogen concentration was significantly increased (p > 05.0) With increased follicular size. A significantly (p> 05.0) correlation was found between both magnesium, zinc ions, and estrogen hormone

Effects of early spontaneous abortions (ESA) and latent toxoplasmosis on Interleukine-23 in women

Cytokines play an important role in intercellular communications, cell growth, differentiation, and immune system modulations. Some of these functions have crucial roles in pregnancy gaining or losing, especially those correlated with T helper 1, T helper 2, and T helper 17 cells. Several studies showed significant variations in Interleukine-23 (IL-23) with Toxoplasma infection. However, little is known about the regulations of this interleukin in the case of early spontaneous abortion (ESA) patients. The present study aimed to evaluate IL-23 to explain the effect of this cytokine on pregnancy gaining or losing with and without toxoplasma infections. Eighty-nine subjects were registered in the current study,16 Toxoplasma infected ESA women, 41 unknown ESA aborted women, 16 healthy pregnant women, and 16 healthy non-pregnant women served as control negative. Detections of anti-Toxoplasma IgG and IL-23 by immunoenzymatic assay (IFA and ELISA, respectively). A significant difference (P = > 0.05) was found in all subjected groups except between Toxoplasmosis ESA and unknown EAS women. The data showed a substantial increase in sera IL-23 in unknown ESA with mean and standard deviation (SD) (13.679±2.461) and Toxoplasma infected women with mean and SD (14.279±4.757) as compared with non-Pregnant women having mean and SD (5.824±1.040) and healthy pregnant women with mean and SD (17.273±6.418). Therefore, this considerable evidence may indicate a role of IL-23 in the development of pregnancy gaining or losing, especially those ESA women.