Study Effect of Infection With Treponema Palladum at the Level of Antibodies and Some Blood Parameters and its Relationship With Blood Factions

صممت هذه الدراسة لتحديد تأثير الإصابة باللولبية الشاحبة Treponema pallidum  على مستوى الأضداد (Ig M, Ig A, Ig G) والمتممة (C3,C4) وبعض معايير الدم لدى الأشخاص المصابين وعلاقة المرض بفصائل الدم في مدينة النجف الاشرف. حيث  اشتملت الدراسة الحالية على30)) حالة إصابة ببكتيريا اللولبية  الشاحبة و20)) حالة من أشخاص غير مصابين ارتادوا مستشفى الزهراء ومدينة الصدر الطبية ومستشفى الحكيم في محافظة النجف الاشرف للمدة من شهر آب2014 ولغاية شهر شباط  2015 في مختبرات مدينة الصدر الطبية. أظهرت الدراسة الحالية حصول زيادة معنوية في مستوى الأضداد Ig M, Ig G)) وكذلك المتممة4 , حسب المعيار المعنوي( P< 0.001 ) في حين أظهرت الدراسة نقص معنوي في مستوى المتممة 3 (P<0.05 ) وكذلك سجلت الدراسة زيادة معنوية في مستوى بعض معايير الدم وهي كريات الدم البيضاء وكريات الدم الحمراء وMPV, HCT في حين سجلت نقص معنوي في نسبة  MCVلدى المصابين مقارنة بمجموعة السيطرة. كما أوضحت الدراسة ان الأشخاص من  فصيلة الدم A  أكثر  عرضة للإصابة تليها فصيلة الدم B وO في حين لم تسجل أي إصابة في فصيلة الدم AB.The study was conducted on 30   patients and 20 healthy people to determine the influences of infected with Treponema pallidum on levels of IgM , IgA, IgG  ,Complement4 ,complement 3 and some Blood parameters in infected with T. Pallidum also relation disease with blood factions  in compared with healthy group who have visited Al-Sadder Medical City and Al-Hakeem Hospital in Al- Najaf governorate during the period from August 2014 till February 2015 in Al-Sadder Medical City Laboratories .The results showed significant increase (P<0.001) in IgM , IgG  ,Complement4 in T. pallidum infected patients in compared to control group. Furthermore the results showed C3 was significant decreased (P< 0.05) in Treponema Pallidum   infected patients in compared to control group also the results showed significant increase in  WBCs, RBCs ,MPV and HCT level and  significant decrease in MCV in patients compared with control group . The study also showed that the people of blood type A are more prone to infection, followed by blood type B and O, while there  are no injuries to the blood type AB

Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System

AIM: To determine toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system. METHODS: Twenty albino female mice were divided equally into two groups: the first group (control) fed with diet pellet. The second group (treated) were inoculated intraperitoneally with a single dose of ATA (100 mg/kg Bw) for 15 days. All mice were sacrificed at the end of the experiment and blood was collected for evaluation of (FSH and LH), serum peroxy nitrate radical concentration. Cytogenetic analysis (chromosomal aberration, micronuclei, mitotic index and blast index) and the histopathological examination on ovary and uterus were done. RESULTS: ATA causes significant reduction (p < 0.0001) for FSH, LH and serum peroxy nitrate radical concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were reported among treated females mainly in uterus tissue and ovary. CONCLUSION: ATA causes significant reduction for FSH, LH and serum peroxy nitrate concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were the main pathological changes in uterus tissue and ovary among treated females

Synthesis and Characterization of Cu(I)-Folic Acid Complex A Theoretical and Experimental Study

Copper (I) complex containing folic acid ligand was prepared and characterized on the basis of metal analyses, UV-VIS, FTIR spectroscopies and magnetic susceptibility. The density functional theory (DFT) as molecular modeling calculations was used to determine the donor atoms of folic acid ligand which appear clearly at oxygen atoms binding to hydrogen. Detection of donation sights is supported by theoretical parameters such as geometry, mulliken population, mulliken charge and HOMO-LUMO gap obtained by DFT calculations

Hopf Bifurcations in a Watt Governor With a Spring

This paper pursues the study carried out by the authors in "Stability and Hopf bifurcation in a hexagonal governor system", focusing on the codimension one Hopf bifurcations in the hexagonal Watt governor differential system. Here are studied the codimension two, three and four Hopf bifurcations and the pertinent Lyapunov stability coefficients and bifurcation diagrams, ilustrating the number, types and positions of bifurcating small amplitude periodic orbits, are determined. As a consequence it is found an open region in the parameter space where two attracting periodic orbits coexist with an attracting equilibrium point.Comment: 30 pages and 7 figure

SUBDUCTED BASALTS AND SEDIMENTS AS SOURCES FOR FELSIC DYKES IN THE MAWAT OPHIOLITE, NE IRAQ

The Mawat ophiolite is a fragment from the Neo-Tethyan oceanic lithosphere and is considered as the largest and best-exposed ophiolite within the Zagros Suture Zone, northeast Iraq. Felsic dykes are encountered in three locations in the Mawat ophiolite. Here, we use field work, petrology and geochemical data to investigate the magma sources of the felsic dykes. The felsic dykes are classified into two types: oceanic plagiogranites in the western (Mirza and Ismail, 2007) and leucogranites in the central (Mohammad et al., 2014) Mawat ophiolite, here called tonalites and granites, respectively. Both types, mixed in various proportions occur in the eastern felsic dykes. The eastern tonalites are weakly peraluminous to metaluminous and are low in K2O (0.13-0.24 wt%) and TiO2 (0.01-0.07 wt%) and high in Na2O (6.63-11.02 wt%). The eastern granites are strongly peraluminous, moderate to high in K2O (1.16-6.57 wt%) and Na2O (2.83-6.47 wt%), and very low in TiO2 (0.03-0.07 wt%). The western tonalites are similar to adakites and are interpreted to have crystallized from melts of subducted oceanic crust interacting with the mantle. The eastern tonalites underwent hornblende and plagioclase fractionation in shallow-level magma chambers modifying their original compositions. The granites are interpreted to derive from melting of psammitic sediments on top of the subducted slab. Parental melts of the tonalities and granites were partially mixed in shallow magma chambers in eastern Mawat.</p

Effects of nano particles on antigen-related airway inflammation in mice

BACKGROUND: Particulate matter (PM) can exacerbate allergic airway diseases. Although health effects of PM with a diameter of less than 100 nm have been focused, few studies have elucidated the correlation between the sizes of particles and aggravation of allergic diseases. We investigated the effects of nano particles with a diameter of 14 nm or 56 nm on antigen-related airway inflammation. METHODS: ICR mice were divided into six experimental groups. Vehicle, two sizes of carbon nano particles, ovalbumin (OVA), and OVA + nano particles were administered intratracheally. Cellular profile of bronchoalveolar lavage (BAL) fluid, lung histology, expression of cytokines, chemokines, and 8-hydroxy-2'-deoxyguanosine (8-OHdG), and immunoglobulin production were studied. RESULTS: Nano particles with a diameter of 14 nm or 56 nm aggravated antigen-related airway inflammation characterized by infiltration of eosinophils, neutrophils, and mononuclear cells, and by an increase in the number of goblet cells in the bronchial epithelium. Nano particles with antigen increased protein levels of interleukin (IL)-5, IL-6, and IL-13, eotaxin, macrophage chemoattractant protein (MCP)-1, and regulated on activation and normal T cells expressed and secreted (RANTES) in the lung as compared with antigen alone. The formation of 8-OHdG, a proper marker of oxidative stress, was moderately induced by nano particles or antigen alone, and was markedly enhanced by antigen plus nano particles as compared with nano particles or antigen alone. The aggravation was more prominent with 14 nm of nano particles than with 56 nm of particles in overall trend. Particles with a diameter of 14 nm exhibited adjuvant activity for total IgE and antigen-specific IgG(1 )and IgE. CONCLUSION: Nano particles can aggravate antigen-related airway inflammation and immunoglobulin production, which is more prominent with smaller particles. The enhancement may be mediated, at least partly, by the increased local expression of IL-5 and eotaxin, and also by the modulated expression of IL-13, RANTES, MCP-1, and IL-6

Mouse models to unravel the role of inhaled pollutants on allergic sensitization and airway inflammation

Air pollutant exposure has been linked to a rise in wheezing illnesses. Clinical data highlight that exposure to mainstream tobacco smoke (MS) and environmental tobacco smoke (ETS) as well as exposure to diesel exhaust particles (DEP) could promote allergic sensitization or aggravate symptoms of asthma, suggesting a role for these inhaled pollutants in the pathogenesis of asthma. Mouse models are a valuable tool to study the potential effects of these pollutants in the pathogenesis of asthma, with the opportunity to investigate their impact during processes leading to sensitization, acute inflammation and chronic disease. Mice allow us to perform mechanistic studies and to evaluate the importance of specific cell types in asthma pathogenesis. In this review, the major clinical effects of tobacco smoke and diesel exhaust exposure regarding to asthma development and progression are described. Clinical data are compared with findings from murine models of asthma and inhalable pollutant exposure. Moreover, the potential mechanisms by which both pollutants could aggravate asthma are discussed

Models of chronic obstructive pulmonary disease

Chronic obstructive pulmonary disease (COPD) is a major global health problem and is predicted to become the third most common cause of death by 2020. Apart from the important preventive steps of smoking cessation, there are no other specific treatments for COPD that are as effective in reversing the condition, and therefore there is a need to understand the pathophysiological mechanisms that could lead to new therapeutic strategies. The development of experimental models will help to dissect these mechanisms at the cellular and molecular level. COPD is a disease characterized by progressive airflow obstruction of the peripheral airways, associated with lung inflammation, emphysema and mucus hypersecretion. Different approaches to mimic COPD have been developed but are limited in comparison to models of allergic asthma. COPD models usually do not mimic the major features of human COPD and are commonly based on the induction of COPD-like lesions in the lungs and airways using noxious inhalants such as tobacco smoke, nitrogen dioxide, or sulfur dioxide. Depending on the duration and intensity of exposure, these noxious stimuli induce signs of chronic inflammation and airway remodelling. Emphysema can be achieved by combining such exposure with instillation of tissue-degrading enzymes. Other approaches are based on genetically-targeted mice which develop COPD-like lesions with emphysema, and such mice provide deep insights into pathophysiological mechanisms. Future approaches should aim to mimic irreversible airflow obstruction, associated with cough and sputum production, with the possibility of inducing exacerbations

Choline deprivation: An overview of the major hepatic metabolic response pathways

Choline (Ch) is an important nutrient that is involved in many physiological functions. Deprivation of Ch (CD) may lead to hepatocellular modifications and/or even hepatic tumorigenesis and it can be a frequent problem in clinical settings; it can accompany various common pathological (alcoholism and malnutrition) or physiological states (pregnancy and lactation). The aim of this review is to provide an up-to-date overview of the major metabolic pathways involved in the hepatic response toward the experimentally or clinically induced CD, and to shed more light on the implicated (and probably interrelated) mechanisms responsible for the observed hepatocellular modifications and/or carcinogenesis. © 2012 Informa Healthcare