Mutation of BRAF V600E in Iraqi Female Patients Diagnosed With Breast Cancer

هدفت هذه الدراسة الى التحري عن وجود طفرة BRAF V600E من عدمها في الاناث التي تم تشخيصهن بالاصابة بسرطان الثدي وفي اعمار مختلفة، حيث تم جمع عينات الدم والانسجة من 46 مريضة وباعمار(46± 3.54)، حيث تم تقسيمها اللى مجموعتين، المجموعة التي  تحت العلاج الكيمياوي وعددها 31 مصابة والمجموعة الاخرى بدون علاج كيمياوي وعددها 15 مصابة كما تم مقارنة النتائج مع مجموعة الاصحاء كمجموعة سيطرة وعددهم 23 شخصا وباعمار(47.93±3.05) سنة.تم اجراء  تفاعل البلمرة المتسلسل ( (PCR وباستخدام بوادئ تم تصميمها ضمن الدراسة الحالية على جميع العينات. اظهرت النتائج عدم وجود علاقة مابين الاصابة بسرطان الثدي وطفرة BRAF V600E في المرضى العراقين المشمولين بالدراسة الحالية.This study aimed to investigate the possible presence of BRAF V600E mutation in the Iraqi female patients who diagnosed with breast cancer in different ages, in which Blood and tissue samples were collected from 46 female patients with  age (46.73±3.54);Those were divided into two groups; who took chemotherapy (31 persons) as treated group and without chemotherapy as an untreated group (15 persons) and from (23) healthy person with age(47.93±3.05).  Polymerase chain reaction (PCR) were done with newly designed primers. The results revealed no correlation between breast cancer occurrence and BRAF V600E mutation in the Iraqi patients enrolled  in the current study. &nbsp

INTERLEUKIN-18 GENE POLYMORPHISM AND SOME RISK FACTORS IN IRAQI PATIENTS WITH BREAST CANCER

Objective: Breast cancer is the most diagnosed cancer in women, which leads to death in a lot of women with breast cancer. The major risk factors associated with breast cancer risk related to family history, age, clinical history, lifestyle factors, long-period hormonal exposure, and single nucleotide polymorphisms in many genes showed possible links with breast cancer incidence risk in different people populations. Our study aimed to figure out the correlation between smoking, lodging and family history, and other factors with the risk of breast cancer.Methods: Blood sample from female patients with breast cancer and healthy individuals were collected and subjected to tetra-amplification refractory mutation system–polymerase chain reaction (T-ARMS-PCR) technique for −607 C/A mutation of an interleukin (IL-18) gene and SPSS 18 software analyzed the results statically.Results: Results showed no association between lodging and smoking with risk of breast cancer, (p>0.05), while the association between the risk and family history were obvious (p<0.05).Conclusion: The results obtained by T-ARMS-PCR technique did not show the association between −607 C/An alternation of IL-18 gene and breast cancer (p>0.05) in the individuals examined in our study.Keywords: Interleukin-18, Gene, Polymorphism, Tetra-amplification refractory mutation system–polymerase chain reaction, Mutations

Efficiency of Plants Extracts Synergism as Antibacterial Activity on Pathogenic Bacteria

Synergism between plant extract was carried out in present study using six common plants which extracted by mixture of methanol: water (20:80)%, then it characterized by thin layer chromatography, antimicrobial activity of these extract performed using OD of bacterial growth at 600 nm  against E.coli , S.aureus , Serratia Spp. , Klebsilla pnemoniae, Aeromonas hydrophillia isolation from different source in  hospital tests were  performed as only one extract on every bacterial species then tow extract was mixed together for evaluated synergist efficiency on every bacterial species, results show that used one extract only causes decreased in bacterial growth in different level, barley was the more effect on pathogenic bacteria but sage was lower effect, it caused activated bacterial growth.Synergism between plant extract also show different effects level  according to mixture forming and types of bacterial species. Present study concluded that mixture of plants used in herbal medicine have disadvantages in addition of its advantage to treatment bacterial infection, in other hand synergism between plants may be more effects. Keywords: synergism , OD, pathogenic bacteria

Some Herbal Medicinal Plants Activity against Candida spp which Resistance to Antifungal Drugs

Some medicinal plant that used as antibacterial in Iraq has been experimented as antifungal, five of these plants include Zingiber officinale, Salvia officinalis, Origanum vulgare, Glycyrrihza glabra, Punica granutum were used against four species of candida spp. C. paracitucus ,C. albicans, C.tropicus ,C.kruzi, which are resistance to antifungal drugs, 80% methanol was used to extract these plants, 5 mg/ml of each extracts used in nutrient broth for 4 days to evaluated anti-candida activity. The Results show that plants extracts causes complete inhibition ,decreased in candida growth ,don’t effect on activated candida growth, Zingiber officinale causes complete inhibition to candida spp except C. albicans, Salvia officinalis was decreased in all candida spp, Origanum vulgare, effect on C. albicans and C, kruzi but it don’t effect on other species. Glycyrrihza glabra decreased all candida spp. Punica granutum decreased growth of C. albicans and C. kruzi only. Keywords: Candida spp , plant extract, 80% methanol

Construction, expression and characterization of TEV protease mutants engineered for improved solubility

In recent years, the highly sequence specific tobacco etch virus protease (TEVp) has emerged as one of the most popular and widely used reagents for removal of fusion tags from target proteins. Its use, however, has been hampered due to relatively poor solubility and inefficient expression in E. coli. Although a lot of progress has been made, there is still need for new and improved TEVp variants. Recently, two different gain-of-function TEVp mutants were described; one containing the substitutions L56V/S135G, which conferred improved solubility and activity in vitro, while the other mutant, containing the substitutions T17S/N68D/I77V, was claimed to yield more soluble protease than the wild-type (wt) protease upon overexpression in E. coli. Here, we analyzed if the L56V/S135G substitutions could promote increased solubility also in vivo, as that would be beneficial to TEVp production and had never been investigated before. We also intended to create a novel, and hopefully superior, TEVp variant with all five mutations combined (T17S/L56V/N68D/S135G/I77V) in a single protease molecule. This variant and the two parental TEVp variants as well as the wt protease, were all expressed in E. coli and characterized with respect to the expression levels, solubility and activity using several different techniques; among them, a newly developed fluorescence-assisted whole-cell assay that directly reports on the apparent protease activity in vivo. Our results show that the L56V/S135G substitutions improve the solubility not only in vitro but also in vivo, which did hold true for the activity as well. Disappointingly, the protease variant containing all five substitutions (T17S/L56V/N68D/S135G/I77V) did not show the best performance, which instead the L56V/S135G variant did. In contrast to an earlier report, we show that the substitutions T17S/N68D/I77V, did not improve the TEVp solubility. In fact, they reduced the activity, and even appeared to have a slightly negative effect on solubility, of all protease constructs in which they were present. Thus, the best current and most promising TEVp variant for future protease engineering efforts, towards improved expression properties and enhanced catalytic efficiency, are those containing the L56V/S135G substitutions.QC 2011051

Construction, expression and characterization of TEV protease mutants engineered for improved solubility

In recent years, the highly sequence specific tobacco etch virus protease (TEVp) has emerged as one of the most popular and widely used reagents for removal of fusion tags from target proteins. Its use, however, has been hampered due to relatively poor solubility and inefficient expression in E. coli. Although a lot of progress has been made, there is still need for new and improved TEVp variants. Recently, two different gain-of-function TEVp mutants were described; one containing the substitutions L56V/S135G, which conferred improved solubility and activity in vitro, while the other mutant, containing the substitutions T17S/N68D/I77V, was claimed to yield more soluble protease than the wild-type (wt) protease upon overexpression in E. coli. Here, we analyzed if the L56V/S135G substitutions could promote increased solubility also in vivo, as that would be beneficial to TEVp production and had never been investigated before. We also intended to create a novel, and hopefully superior, TEVp variant with all five mutations combined (T17S/L56V/N68D/S135G/I77V) in a single protease molecule. This variant and the two parental TEVp variants as well as the wt protease, were all expressed in E. coli and characterized with respect to the expression levels, solubility and activity using several different techniques; among them, a newly developed fluorescence-assisted whole-cell assay that directly reports on the apparent protease activity in vivo. Our results show that the L56V/S135G substitutions improve the solubility not only in vitro but also in vivo, which did hold true for the activity as well. Disappointingly, the protease variant containing all five substitutions (T17S/L56V/N68D/S135G/I77V) did not show the best performance, which instead the L56V/S135G variant did. In contrast to an earlier report, we show that the substitutions T17S/N68D/I77V, did not improve the TEVp solubility. In fact, they reduced the activity, and even appeared to have a slightly negative effect on solubility, of all protease constructs in which they were present. Thus, the best current and most promising TEVp variant for future protease engineering efforts, towards improved expression properties and enhanced catalytic efficiency, are those containing the L56V/S135G substitutions.QC 2011051

Construction, expression and characterization of TEV protease mutants engineered for improved solubility

In recent years, the highly sequence specific tobacco etch virus protease (TEVp) has emerged as one of the most popular and widely used reagents for removal of fusion tags from target proteins. Its use, however, has been hampered due to relatively poor solubility and inefficient expression in E. coli. Although a lot of progress has been made, there is still need for new and improved TEVp variants. Recently, two different gain-of-function TEVp mutants were described; one containing the substitutions L56V/S135G, which conferred improved solubility and activity in vitro, while the other mutant, containing the substitutions T17S/N68D/I77V, was claimed to yield more soluble protease than the wild-type (wt) protease upon overexpression in E. coli. Here, we analyzed if the L56V/S135G substitutions could promote increased solubility also in vivo, as that would be beneficial to TEVp production and had never been investigated before. We also intended to create a novel, and hopefully superior, TEVp variant with all five mutations combined (T17S/L56V/N68D/S135G/I77V) in a single protease molecule. This variant and the two parental TEVp variants as well as the wt protease, were all expressed in E. coli and characterized with respect to the expression levels, solubility and activity using several different techniques; among them, a newly developed fluorescence-assisted whole-cell assay that directly reports on the apparent protease activity in vivo. Our results show that the L56V/S135G substitutions improve the solubility not only in vitro but also in vivo, which did hold true for the activity as well. Disappointingly, the protease variant containing all five substitutions (T17S/L56V/N68D/S135G/I77V) did not show the best performance, which instead the L56V/S135G variant did. In contrast to an earlier report, we show that the substitutions T17S/N68D/I77V, did not improve the TEVp solubility. In fact, they reduced the activity, and even appeared to have a slightly negative effect on solubility, of all protease constructs in which they were present. Thus, the best current and most promising TEVp variant for future protease engineering efforts, towards improved expression properties and enhanced catalytic efficiency, are those containing the L56V/S135G substitutions.QC 2011051

Association of adiponectin gene polymorphism and cytokines levels in obese patients with COVID-19

Covid-19 is a genus of enveloped, positive-sense, single-stranded RNA viruses with a high degree of genetic diversity. They induce a variety of disorders affecting the respiratory, gastrointestinal, hepatic, and nervous systems in humans and animals, with different manifestation and severity. Several risk variables, including older age, male gender, type 2 diabetes mellitus, hypertension, coronary artery disease, and obesity (a higher body mass index (BMI)), are known to predict a worse course of covd-19 infection. Obesity is the result of the accumulation of an excessive amount of fat in the body and the condition arises from an imbalance between the amount of energy stored by increased food intake and the amount of energy expended as physical activity. The aims of study evaluate the effect of covid-19 on cytokines levels in obese patients. As well as, study the association of adiponectin gene polymorphism to the risk of infection with covid-19 in obese patients.&nbsp